Assuntos
Queimaduras por Corrente Elétrica/cirurgia , Traumatismos do Antebraço/cirurgia , Ilustração Médica , Modelos Anatômicos , Procedimentos de Cirurgia Plástica/métodos , Interface Usuário-Computador , Adulto , Simulação por Computador , Traumatismos do Antebraço/etiologia , Humanos , Imageamento Tridimensional/métodos , Masculino , Retalhos CirúrgicosRESUMO
Chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and high frequency cells (HFCs) have been assessed in peripheral blood lymphocytes of 10 neurofibromatosis (NF1) patients and 10 healthy controls. In both groups, the spontaneous rates and the induced (bleomycin for CA and MMC for SCE) frequencies were analyzed. No differences between cells from NF1 patients and controls were observed with respect to spontaneous or bleomycin induced CA. Spontaneous or MMC induced SCE frequencies were also similar in NF1 patients and controls. HFCs, on the contrary, were statistically lower in NF1 patients.
Assuntos
Aberrações Cromossômicas , Neurofibromatose 1/genética , Troca de Cromátide Irmã , Adolescente , Antimetabólitos Antineoplásicos/toxicidade , Bleomicina/toxicidade , Criança , Pré-Escolar , Fragilidade Cromossômica , Feminino , Humanos , Lactente , Masculino , Mitomicina/toxicidade , Neurofibromatose 1/sangue , Neurofibromatose 1/patologia , Inibidores da Síntese de Ácido Nucleico/toxicidade , Troca de Cromátide Irmã/efeitos dos fármacosRESUMO
Deposition of free iron is a characteristic feature of beta-thalassemia (beta-thal) red blood cells believed to play an important role in the generation of oxidative injury to the cell membrane. Increased red blood cell KCI cotransport, reduced K content, and cell dehydration are also found in beta-thal red blood cells. It is not known, however, whether deposition of free iron plays a role in these membrane transport changes. To explore this issue, we studied-both in vitro and in vivo-the effect on KCI cotransport of removing red blood cell membrane free iron from beta-thal erythrocytes. Eleven patients with beta-thal major who underwent long-term transfusion and were treated with deferiprone (75 mg/kg/day) for 9 months participated in the study. Deferiprone therapy removed membrane free iron from beta-thal erythrocytes, which was followed by reduced KCI cotransport activity. The reduced KCI cotransport activity was accompanied by an increase in the red blood cell K content. These data suggest that the increased activity of KCI cotransport in beta-thal red blood cells is mediated by the deposition of membrane free iron, a mechanism that may be attenuated by deferiprone therapy.