Do the Choice of Fusion Construct With and Without Autograft Influence the Fusion and Complication Rates in Patients Undergoing 1 or 2-Level Anterior Cervical Discectomy and Fusion Surgery? A PRISMA-Compliant Network Meta-Analysis.

STUDY DESIGN: Network meta-analysis. OBJECTIVES: To compare the fusion outcome and complications of different 1 or 2-level anterior cervical decompression and fusion (ACDF) constructs performed with and without the application of autografts. METHODS: We performed an independent and duplicate search in electronic databases including PubMed, Embase, Web of Science, Cochrane, and Scopus for relevant articles published between 2000 and 2020. We included comparative studies reporting fusion rate and complications with and without the use of autografts in ACDF across 5 different fusion constructs. A network meta-analysis was performed in Stata, categorized based on the type of fusion constructs utilized. Fusion constructs were ranked based on p-score approach and surface under cumulative ranking curve (SUCRA) scores. The confidence of results from the analysis was appraised with Cochrane's CINeMA approach. RESULTS: A total of 2216 patients from 22-studies including 6 Randomized Controlled Trials (RCTs) and 16 non-RCTs were included in network analysis. The mean age of included patients was 49.3 (±3.62) years. Based on our meta-analysis, we could conclude that use of autograft in 1- or 2-level ACDF did not affect the fusion and mechanical implant-related complications. The final fusion and mechanical complication rates were also not significantly different across the different fusion constructs. The use of plated constructs was associated with a significant increase in post-ACDF dysphagia rates [OR 3.42; 95%CI (.01,2.45)], as compared to stand-alone constructs analysed. CONCLUSION: The choice of fusion constructs and use of autografts does not significantly affect the fusion and overall complication rates following 1 or 2-level ACDF surgery.

Risk factors for recurrence in blunt traumatic abdominal wall hernias: A secondary analysis of a Western Trauma association multicenter study.

BACKGROUND: Few studies have investigated risk factors for recurrence of blunt traumatic abdominal wall hernias (TAWH). METHODS: Twenty trauma centers identified repaired TAWH from January 2012 to December 2018. Logistic regression was used to investigate risk factors for recurrence. RESULTS: TAWH were repaired in 175 patients with 21 (12.0%) known recurrences. No difference was found in location, defect size, or median time to repair between the recurrence and non-recurrence groups. Mesh use was not protective of recurrence. Female sex, injury severity score (ISS), emergency laparotomy (EL), and bowel resection were associated with hernia recurrence. Bowel resection remained significant in a multivariable model. CONCLUSION: Female sex, ISS, EL, and bowel resection were identified as risk factors for hernia recurrence. Mesh use and time to repair were not associated with recurrence. Surgeons should be mindful of these risk factors but could attempt acute repair in the setting of appropriate physiologic parameters.

Management of blunt traumatic abdominal wall hernias: A Western Trauma Association multicenter study.

BACKGROUND: Blunt traumatic abdominal wall hernias (TAWH) occur in approximately 15,000 patients per year. Limited data are available to guide the timing of surgical intervention or the feasibility of nonoperative management. METHODS: A retrospective study of patients presenting with blunt TAWH from January 2012 through December 2018 was conducted. Patient demographic, surgical, and outcomes data were collected from 20 institutions through the Western Trauma Association Multicenter Trials Committee. RESULTS: Two hundred and eighty-one patients with TAWH were identified. One hundred and seventy-six (62.6%) patients underwent operative hernia repair, and 105 (37.4%) patients underwent nonoperative management. Of those undergoing surgical intervention, 157 (89.3%) were repaired during the index hospitalization, and 19 (10.7%) underwent delayed repair. Bowel injury was identified in 95 (33.8%) patients with the majority occurring with rectus and flank hernias (82.1%) as compared with lumbar hernias (15.8%). Overall hernia recurrence rate was 12.0% (n = 21). Nonoperative patients had a higher Injury Severity Score (24.4 vs. 19.4, p = 0.010), head Abbreviated Injury Scale score (1.1 vs. 0.6, p = 0.006), and mortality rate (11.4% vs. 4.0%, p = 0.031). Patients who underwent late repair had lower rates of primary fascial repair (46.4% vs. 77.1%, p = 0.012) and higher rates of mesh use (78.9% vs. 32.5%, p < 0.001). Recurrence rate was not statistically different between the late and early repair groups (15.8% vs. 11.5%, p = 0.869). CONCLUSION: This report is the largest series and first multicenter study to investigate TAWHs. Bowel injury was identified in over 30% of TAWH cases indicating a significant need for immediate laparotomy. In other cases, operative management may be deferred in specific patients with other life-threatening injuries, or in stable patients with concern for bowel injury. Hernia recurrence was not different between the late and early repair groups. LEVEL OF EVIDENCE: Therapeutic/care management, Level IV.

Awake Mapping of the Auditory Cortex during Tumor Resection in an Aspiring Musical Performer: A Case Report.

INTRODUCTION: Preoperative functional MRI (fMRI) and intraoperative awake cortical mapping are established strategies to identify and preserve critical language structures during neurosurgery. There is growing appreciation for the need to similarly identify and preserve eloquent tissue critical for music production. CASE REPORT: A 19-year-old female musician, with a 3- to 4-year history of events concerning for musicogenic seizures, was found to have a right posterior temporal tumor, concerning for a low-grade glial neoplasm. Preoperative fMRI assessing passive and active musical tasks localized areas of activation directly adjacent to the tumor margin. Cortical stimulation during various musical tasks did not identify eloquent tissue near the surgical site. A gross total tumor resection was achieved without disruption of singing ability. At 9-month follow-up, the patient continued to have preserved musical ability with full resolution of seizures and without evidence of residual lesion or recurrence. CONCLUSION: A novel strategy for performing an awake craniotomy, incorporating preoperative fMRI data for music processing with intraoperative cortical stimulation, interpreted with the assistance of a musician expert and facilitated gross total resection of the patient's tumor without comprising her musical abilities.

Bilateral paraspinal muscle flap closure technique for reduction of wound complications from posterior thoracolumbar spinal fusion: results of a series of 716 patients.

OBJECTIVE: Wound complications such as surgical site infection (SSI) and dehiscence are among the most common complications of thoracolumbar spinal fusion surgery and are particularly prevalent in patients with risk factors such as obesity, diabetes, smoking, malignancy, and multilevel and/or revision procedures. A specialized wound closure technique with muscle flap mobilization, which reduces tension at the wound edges and increases the bulk of vascularized tissue in the midline, can be employed as a salvage procedure to manage wound complications. The authors evaluated the effectiveness of prophylactic muscle flap closure for reducing SSI in patients with risk factors for wound complications who undergo thoracolumbar fusion surgery. METHODS: A retrospective review of thoracolumbar fusion surgeries over a 15-year period was conducted in a group of patients at risk for wound complications to compare outcomes of patients who underwent prophylactic muscle flap closure with outcomes of patients who had conventional wound closure. Patients were selected for specialized closure based upon a protocol adopted during the study period. Patients were excluded if they had active infections or underwent tubular retractor-mediated decompression and did not have open surgery with a midline incision. RESULTS: Of 716 patients, wound closure was performed in 455 patients using conventional closure and in 261 using muscle flap closure. There were no significant differences in the ratios of male to female patients, with 251 men and 204 women with conventional closure and 133 men and 128 women with muscle flap closure, but the muscle flap patients were older than the conventional closure patients, with mean ages of 65.2 versus 62.9 years (p < 0.005). Indications for surgery in the muscle flap group and the conventional group, respectively, were metastatic disease in 44 (17%) and 32 (7%) patients; trauma in 10 (4%) and 14 (3%) patients; and degenerative disease, including spondylolisthesis, spondylolysis, and stenosis, in 207 (79%) and 409 (90%) patients, with more muscle flap patients having metastasis (p < 0.00001). Patients having muscle flaps had significantly higher rates of diabetes, smoking, and revision surgery, and a higher mean BMI and number of operative levels. The serum albumin level was slightly lower in the muscle flap group (p < 0.047). The wound infection rate was significantly lower in the muscle flap group (0.4%) compared with the conventional closure group (2.4%) (p < 0.033). CONCLUSIONS: Prophylactic muscle flap closure significantly lowers the rate of SSI in patients undergoing thoracolumbar spinal fusion who harbor risk factors for wound complications, with even fewer infections seen than in a group of patients without similar risk factors. Given the success of the technique, consideration of wider use for thoracolumbar fusion cases, even those without a high level of complexity, may be warranted.

Patterns of neurological deficits and recovery of postoperative C5 nerve palsy.

OBJECTIVE: Paresis of the C5 nerve is a well-recognized complication of cervical spine surgery. Numerous studies have investigated its incidence and possible causes, but the specific pattern and character of neurological deficits, time course, and relationship to preoperative cord signal changes remain incompletely understood. METHODS: Records of patients undergoing cervical decompressive surgery for spondylosis, disc herniation, or ossification of the longitudinal ligament, including the C4-5 level, were reviewed from a 15-year period, identifying C5 palsy cases. Data collected included age, sex, diabetes and smoking statuses, body mass index, surgical levels, approach, presence of increased cord signal intensity, and modified Japanese Orthopaedic Association (mJOA) scores. Narrative descriptions of the patterns and findings on neurological examination were reviewed, and complications were noted. The minimum follow-up requirement for the study was 12 months. RESULTS: Of 642 patients who underwent cervical decompressive surgery, 18 developed C5 palsy (2.8%). The incidence was significantly lower following anterior surgery (6 of 441 [1.4%]) compared with that following cervical laminectomy and fusion (12 of 201 [6.0%]) (p < 0.001). There were 10 men and 8 women whose mean age was 66.7 years (range 54-76 years). The mean preoperative mJOA score of 11.4 improved to 15.6 at the latest follow-up examination. There were no differences between those with and without C5 palsy with regard to sex, age, number of levels treated, or pre- or postoperative mJOA score. Fifteen patients with palsy (83%) had signal changes/myelomalacia on preoperative T2-weighted imaging, compared with 436 of 624 (70%) patients without palsy; however, looking specifically at the C4-5 level, signal change/myelomalacia was present in 12 of 18 (67%) patients with C5 palsy, significantly higher than in the 149 of 624 (24%) patients without palsy (p < 0.00003). Paresis was unilateral in 16 (89%) and bilateral in 2 (11%) patients. All had deltoid weakness, but 15 (83%) exhibited new biceps weakness, 8 (44%) had triceps weakness, and 2 (11%) had hand intrinsic muscle weakness. The mean time until onset of palsy was 4.6 days (range 2-14 days). Two patients (11%) complained of shoulder pain preceding weakness; 3 patients (17%) had sensory loss. Recovery to grade 4/5 deltoid strength occurred in 89% of the patients. No patient had intraoperative loss of somatosensory or motor evoked potentials or abnormal intraoperative C5 electromyography activity. CONCLUSIONS: Postoperative C5 nerve root dysfunction appears in a delayed fashion, is predominantly a motor deficit, and weakness is frequently appreciated in the biceps and triceps muscles in addition to the deltoid muscle. Preoperative cord signal change/myelomalacia at C4-5 was a significant risk factor. No patient had a detectable deficit in the immediate postoperative period or changes in intraoperative neuromonitoring status. Neurological recovery to at least that of grade 4/5 occurred in nearly 90% of the patients.

Unilateral Snapping Biceps Femoris Tendon with an Anomalous Insertion Treated with Anatomic Repositioning and Lengthening with a Single Suture Anchor: A Report of Two Cases.

CASE: A snapping biceps femoris tendon, a rare etiology for symptomatic pain in the lateral aspect of the knee, has been reported infrequently. We report 2 cases of an acutely symptomatic unilateral snapping biceps femoris tendon with an intraoperatively intact anomalous insertion onto the tibia, which were treated with anatomic repositioning and "pie-crust" lengthening via a single suture anchor. CONCLUSION: Anatomic repositioning and lengthening is a novel technique that successfully resolved the painful snapping and allowed full return to competitive sports by 6 months postoperatively, with maintenance of improvement at ≥14 months postoperatively.

Comparison of Surgical Outcomes, Narcotics Utilization, and Costs After an Anterior Cervical Discectomy and Fusion: Stand-alone Cage Versus Anterior Plating.

STUDY DESIGN: Retrospective cohort analysis of a prospectively maintained registry. OBJECTIVE: To compare the surgical outcomes, narcotic utilization, and costs between a stand-alone (SA) cage and anterior plating (AP) with an interbody device for 1-level anterior cervical discectomy and fusion (ACDF). BACKGROUND DATA: ACDF with a SA cage has gained popularity as a potential alternative to anterior cervical plating. Few studies have compared the surgical outcomes, narcotic utilization, and costs of ACDF utilizing a SA cage versus AP with an interbody device. METHODS: Patients who underwent a primary 1-level ACDF for degenerative spinal pathology between 2010 and 2013 were analyzed. Patients were stratified on the basis of the type of implant system (SA cage vs. AP) and assessed with regard to demographics, comorbidities, smoking, visual analogue scale (VAS) scores (preoperative/postoperative), procedural time, estimated blood loss (EBL), length of hospitalization, complications, reoperations, narcotic consumption, and total costs. Statistical analysis was performed with independent sample T tests for continuous variables and χ analysis for categorical data. An α level of <0.05 denoted statistical significance. RESULTS: Of the 93 patients included, 52 (55.9%) underwent an ACDF with a SA cage system. Patient demographics, comorbidity burden, body mass index, smoking status, and preoperative VAS score were similar between cohorts. The SA cohort incurred a significantly lower EBL (P<0.001) than the AP cohort. However, none required a transfusion and the procedural time, length of hospitalization, postoperative VAS score, complication rates, 1-year arthrodesis rate, and reoperation rates were similar between cohorts. Postoperative narcotics consumption and total costs were also similar between groups. CONCLUSIONS: Our findings suggest that the SA cage may be associated with a significantly lower EBL, which may not be clinically relevant. Perioperative outcomes, complications, reoperation rates, narcotics consumption in the immediate postoperative period, and total costs may be similar regardless of the instrumentation utilized in a 1-level ACDF.

Dial Test: Unrecognized Predictor of Anterior Cruciate Ligament Deficiency.

PURPOSE: To evaluate the effect of isolated anterior cruciate ligament (ACL) injury on tibial external rotation as measured by the dial test. METHODS: Twenty-seven consecutive patients scheduled to undergo a primary ACL reconstruction were prospectively evaluated. Physical examination and magnetic resonance imaging findings were analyzed to exclude multiligamentous injury. The dial test was performed with the patient under anesthesia with a goniometer on both the affected and unaffected knees at 30° and 90°. Intraoperatively, the arthroscopic posterolateral corner gaps before reconstruction and after reconstruction were documented. Postoperatively, the dial test was again performed on both knees at 30° and 90°. RESULTS: At 30°, there was a significantly larger dial test result in the affected knee before ACL reconstruction compared with after ACL reconstruction (27.6° vs 21.0°, P < .0001) and compared with the unaffected knee (27.6° vs 20.5°, P < .0001), but this difference was eliminated after reconstruction (21.0° vs 20.5°, P = .5089). At 90°, there was a significantly larger dial test result in the affected knee before ACL reconstruction compared with after ACL reconstruction (27.6° vs 21.1°, P < .0001) and compared with the unaffected knee (27.6° vs 20.9°, P < .0001), with this difference was eliminated after reconstruction (21.1° vs 20.9°, P = .7831). CONCLUSIONS: Incompetence of the ACL accounts for nearly 7° of tibial external rotation found by the dial test. During examination of an injured knee, if the dial test is positive, an isolated ACL injury should not be excluded. Findings of the dial test should thus be interpreted with caution in the setting of ACL injury. LEVEL OF EVIDENCE: Level II, prospective comparative study.

Arthroscopic Repair of Isolated Subscapularis Tears: A Systematic Review of Technique-Specific Outcomes.

PURPOSE: To systematically review the literature to identify all studies reporting outcomes of arthroscopically repaired isolated subscapularis tears, to (1) report outcomes across all repair techniques, (2) compare outcomes by arthroscopic technique, and (3) highlight the frequency and management of associated long head of biceps pathology, and the influence of these concomitant procedures on outcomes following arthroscopic subscapularis repair. METHODS: A systematic literature review was conducted using the MEDLINE, Embase, and Scopus databases with the following term: ("isolated repair" AND "arthroscopic subscapularis tear"). Only studies evaluating the techniques and outcomes of isolated subscapularis repair were included. Data were extracted, including patient characteristics, surgical technique, and outcomes. Descriptive analysis was provided for the available literature. RESULTS: Eight studies were included in this review. Uniformly, improvements in patient-reported outcome scores were substantial after arthroscopic subscapularis repair. Constant Total scores improved in each individual study from preoperative to postoperative (range, Δ18.8-Δ49.8 points), as did Strength (range, Δ1.3-Δ13.7 points), Pain (range, Δ7.6-Δ8.9 points), Range of Motion (range, Δ7.3-Δ13.3 points), and Activities of Daily Living (range, Δ8.7-Δ10.2 points) subscores. Significant improvements were seen in most individual studies for belly-press (Δ21.6 N or Δ1.9 out of 5) and lift-off strength (Δ24.3 N or Δ1.7-Δ1.9 out of 5), range of motion in forward flexion (29.1°-37.0°), external rotation (10.3°-16.0°), and internal rotation. Complications were relatively infrequent overall, with 5 studies reporting no complications, and the remaining 3 studies with rerupture rates between 4.8% and 11.8%. Studies that used only double-row repair reported fewer complications (0% vs 5%-10%) and better outcome scores than single-row repair, similar to those studies that uniformly performed biceps tenodesis compared with no biceps intervention. CONCLUSIONS: This descriptive study highlights that arthroscopic subscapularis repair appears to be a reasonable option for the treatment of isolated tears of the subscapularis to obtain successful functional and patient-reported clinical outcomes. Its findings also pose the question of whether future prospective, comparative studies will find double-row surgical fixation and concomitant biceps tenodesis surgery to be superior to single-row fixation and leaving the biceps alone. LEVEL OF EVIDENCE: Level IV, systematic review of Level IV studies.

Optimization of Anteromedial Portal Femoral Tunnel Drilling With Flexible and Straight Reamers in Anterior Cruciate Ligament Reconstruction: A Cadaveric 3-Dimensional Computed Tomography Analysis.

PURPOSE: To use 3-dimensional custom CAD technology to evaluate how knee flexion angle affects femoral tunnel length and distance to the posterior wall when using curved and straight guides for drilling through the anteromedial portal (AMP). METHODS: Six cadaveric knees were placed in an external fixator at various degrees of flexion (90°, 110°, 125°, and maximum 135° to 140°). Computed tomography scans were obtained at all flexion points for 3-dimensional point-cloud models. Using custom CAD software, surgical guides through the AMP were replicated along with virtual tunnels at each flexion angle. Distance from the posterior cortex and tunnel dimensions were collected after 8-mm and 10-mm tunnel creation. RESULTS: At 90° of flexion, the average tunnel length down the posterior aspect of 8-mm tunnel was 25.0 mm (95% confidence interval [CI] 16.2-33.8) and 12.0 mm (95% CI 7.3-16.7) for curved and straight guides, respectively; 31.0 mm (95% CI 26.8-35.2) and 28.6 mm (95% CI 24.8-32.4) at 110°; 33.8 mm (95% CI 30.1-37.5) and 31.1 mm (95% CI 26.8-35.4) at 125°; and 35.0 mm (95% CI 34.1-35.9) and 35.5 mm (95% CI 34.2-36.8) with maximal flexion. Values between curved and straight guides are significantly different (P < .001), with straight guides breaching the posterior wall at 90° and 110° of flexion in some specimens. The average distance to the posterior wall cortex was 0.9 mm (95% CI -1.5 to 3.3) and -0.6 mm (95% CI -2.3 to 1.1) for curved and straight guides, respectively, at 90° of flexion (P = .014); 2.3 mm (95% CI -0.2 to 4.8) and -0.1 mm (95% CI -2.4 to 2.2) at 110° (P = .001); 4.4 mm (95% CI 2.8-6.0) and 3.9 mm (95% CI 1.9-5.9) at 125° (P = .299); and 6.7 mm (95% CI 6.2-7.2) and 8.3 mm (95% CI 6.1-10.5) at maximal flexion (P = .184). Posterior wall blowout was noted when using 10-mm straight guides at both 90° (2 specimens) and 110° (3 specimens). Using 10-mm curved guides posterior blowout was noted in 1 specimen at 90°. Maximum footprint coverage occurred at 110° for straight guides and 90° for curved guides. CONCLUSIONS: When using the AMP, flexible guides and reamers result in a greater distance of the tunnel to the femoral cortex while preserving adequate tunnel length at lower knee flexion angles. To create long femoral tunnels without breaching the posterior cortex, the knee should be flexed to at least 110° for curved reamers and 125° for straight. CLINICAL RELEVANCE: Femoral tunnel drilling through the AMP using curved and straight reamers requires different degrees of knee flexion to achieve optimal tunnel dimensions.

Publication Rates of Podium Versus Poster Presentations at the Arthroscopy Association of North America Meetings 2008-2012.

PURPOSE: To analyze the publication rate of poster and podium presentations at Arthroscopy Association of North America (AANA) annual meetings from 2008 to 2012. METHODS: An online search using PubMed and Google Scholar for all published manuscripts associated with abstracts presented from 2008 to 2012 AANA annual meetings was performed. Abstracts were classified by presentation type (poster vs podium), and the journal and publication date were recorded for all published abstracts. Descriptive statistics, logistic regression, and Fisher's exact tests were performed, with P < .05 considered significant. RESULTS: A total of 1,508 abstracts were submitted to AANA annual meetings from 2008 to 2012, with 976 abstracts accepted for presentation (65% overall acceptance rate). There were 328 podium (22% acceptance rate) and 648 poster (43% acceptance rate) presentations. Of the 976 accepted abstracts, 479 (49%) were published within 3 years in peer-reviewed journals. The overall publication rates for podium and poster presentations were 59% (n = 193) and 44% (n = 286), respectively. Podium presentations were significantly more likely to be published within 3 years compared with poster presentations (P < .0001; odds ratio 1.8095, confidence interval 1.3826-2.3682). There were no differences in time to publication between podium and poster presentations (1.3 ± 1.2 vs 1.1 ± 1.3 years, P = .0633). Over the 5-year study period, the overall abstract acceptance rate (P < .0001) and the rate of abstracts accepted for poster presentation (P < .0001) increased significantly over time, whereas there was no increase in the rate of abstracts accepted for podium presentation (P = .5638). The most common journals of publication were Arthroscopy (n = 157, 32.7%) followed by American Journal of Sports Medicine (n = 93, 19.4%). CONCLUSIONS: The overall publication rate of abstracts presented at AANA annual meetings is 49%, with podium presentations 1.8 times more likely to be published than poster presentations. The overall abstract acceptance rate and the rate of abstracts accepted for poster presentation increased significantly over time, whereas there was no significant increase in the rate of abstracts accepted for podium presentation. CLINICAL RELEVANCE: The publication rates of abstracts presented at the AANA annual meetings demonstrate the meetings' impact and importance to the advancement of the scientific literature.

Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase.

OBJECTIVE: Vein graft adaptation is characterized by loss of expression of the tyrosine kinase receptor Eph-B4, the embryonic determinant of venous identity, without increased expression of its ligand ephrin-B2, the embryonic determinant of arterial identity. Endothelial nitric oxide synthase (eNOS) is an important mediator of vessel remodeling. We hypothesized that the mechanism of action of Eph-B4 during vein graft adaptation might be through regulation of downstream eNOS activity. METHODS: Mouse lung endothelial cells were stimulated with ephrin-B2/Fc, without and with preclustering, without and with the eNOS inhibitor Nω-nitro-l-arginine methyl ester hydrochloride or the Eph-B4 inhibitor NVP-BHG712, and assessed by Western blot and immunofluorescence for eNOS and Eph-B4 phosphorylation. Nitric oxide (NO) production was assessed using an NO-specific chemiluminescence analyzer. Cell migration was assessed using a Transwell assay. Human and mouse vein graft specimens were examined for eNOS activity by Western blot, and vessel remodeling was assessed in vein grafts in wild-type or eNOS knockout mice. RESULTS: Ephrin-B2/Fc stimulated both Eph-B4 and eNOS phosphorylation in a bimodal temporal distribution (n = 4; P < .05), with preclustered ephrin-B2/Fc causing prolonged peak Eph-B4 and eNOS phosphorylation as well as altered subcellular localization (n = 4; P < .05). Ephrin-B2/Fc increased NO release (n = 3; P < .01) as well as increased endothelial cell migration (n = 6; P < .05) in an eNOS-dependent fashion. Both human and mouse vein grafts showed increased eNOS phosphorylation compared with normal veins (n = 3; P < .05). Vein grafts from eNOS knockout mice showed less dilation and less wall thickening compared with wild-type vein grafts (n = 7; P < .05). CONCLUSIONS: eNOS is a mediator of vein graft adaptation to the arterial environment. Eph-B4 stimulates eNOS phosphorylation in vitro and may mediate vein graft adaptation by regulation of eNOS activity in vivo.

Publication rates of podium presentations at the American Shoulder and Elbow Surgeons annual open versus closed meetings 2008 to 2012.

AIM: The purpose of this study was to analyze the publication rate for abstracts presented at podium presentations from the American Shoulder and Elbow Surgeons (ASES) annual open and closed meetings from 2008 to 2012. MATERIALS AND METHODS: Abstracts accepted as podium presentations for the open and closed meetings from 2008 through 2012 were followed. A search was performed using Google Scholar and PubMed for all published manuscripts. This analysis looks at abstracts categorized based on annual meeting (open versus closed) and by meeting year (2008-2012). Data including publication journal, publication date, and level of evidence were recorded. Descriptive statistics, t-tests, and odds ratios were performed with p < 0.05 significance. RESULTS: A total of 365 abstracts were accepted to the open and closed annual meetings from 2008 to 2012, with 49% and 51% presented in open and closed forums. A total of 222 (61%) were published within 3-years in peer-reviewed journals. No difference existed in 3-year publication rate between open and closed podium presentation meetings (112/178, 63% open; 110/187, 59% closed; p = 0.4229); however, presentations at closed meetings were more likely to be published after 3-years compared to open meetings (2/178, 2% open; 15/187, 12% closed; p = 0.002). Most common journal of publication was the Journal of Shoulder and Elbow Surgery (JSES) (50%). CONCLUSIONS: Podium abstracts presented at the open and closed annual meetings have publication rates of 63% and 59% with overall combined publication rates of 61% from 2008 to 2012. The high publication rate and high impact of publications speak to the exemplary educational value of ASES annual meetings.

Growth Abnormalities Following Anterior Cruciate Ligament Reconstruction in the Skeletally Immature Patient: A Systematic Review.

PURPOSE: To identify all reported cases of growth disturbances after anterior cruciate ligament (ACL) reconstruction in patients with open growth plates and analyze trends with respect to different surgical techniques, graft choices, and methods of fixation. METHODS: A systematic literature review was conducted using the MEDLINE, EMBASE, and SCOPUS databases with the following term: "((anterior cruciate ligament OR ACL) AND ((((immature) OR growth plates) OR physes) OR pediatric))." Only studies that evaluated ACL reconstruction in patients with open growth plates and reported angular malformations or limb length discrepancy were included. Data were extracted, including patient characteristics, surgical technique, and postoperative growth disturbance. RESULTS: Twenty-one studies containing 39 patients with growth abnormalities were included in the review. Mean chronological age was 13 years, and 89% of patients were male. Overall, there were 16 cases of angular malformations and 29 cases of limb length discrepancy. The most common angular malformation was genu valgum (81%, n = 13; mean of 6.5°). The most common surgical technique on the tibia and femur was transphyseal (54%, and 77% respectively), and the most common graft used was hamstring autograft (58%). Among patients with limb length discrepancy, overgrowth was most common (62%, n = 18; mean of 13 mm). Interestingly, we observed that 50% of patients with overgrowth underwent a physeal-sparing technique, whereas 64% of patients with shortening underwent a transphyseal technique. CONCLUSIONS: At present, there are 21 studies reporting 39 patients with growth abnormalities in the current literature, of which 29 cases were of limb length discrepancy and 16 of angular malformation. Of the 29 cases of limb length discrepancy, limb overgrowth accounted for 62% of cases. Perhaps most interestingly, physeal-sparing techniques were performed in 25% of the cases of angular malformation and 47% cases of limb length discrepancy, despite the commonly held belief that this technique mitigates the risks of ACL reconstruction by not violating the growth plate. According to this study, it is clear that growth abnormalities after ACL reconstruction in the skeletally immature patient are underreported, and our current understanding of the etiology of these abnormalities is limited. LEVEL OF EVIDENCE: Level IV, systematic review of Level II to IV studies.

The visual and functional impacts of astigmatism and its clinical management.

PURPOSE: To provide a comprehensive overview of research examining the impact of astigmatism on clinical and functional measures of vision, the short and longer term adaptations to astigmatism that occur in the visual system, and the currently available clinical options for the management of patients with astigmatism. RECENT FINDINGS: The presence of astigmatism can lead to substantial reductions in visual performance in a variety of clinical vision measures and functional visual tasks. Recent evidence demonstrates that astigmatic blur results in short-term adaptations in the visual system that appear to reduce the perceived impact of astigmatism on vision. In the longer term, uncorrected astigmatism in childhood can also significantly impact on visual development, resulting in amblyopia. Astigmatism is also associated with the development of spherical refractive errors. Although the clinical correction of small magnitudes of astigmatism is relatively straightforward, the precise, reliable correction of astigmatism (particularly high astigmatism) can be challenging. A wide variety of refractive corrections are now available for the patient with astigmatism, including spectacle, contact lens and surgical options. CONCLUSION: Astigmatism is one of the most common refractive errors managed in clinical ophthalmic practice. The significant visual and functional impacts of astigmatism emphasise the importance of its reliable clinical management. With continued improvements in ocular measurement techniques and developments in a range of different refractive correction technologies, the future promises the potential for more precise and comprehensive correction options for astigmatic patients.

The Nogo-B-PirB axis controls macrophage-mediated vascular remodeling.

OBJECTIVE: Nogo-B mediates vascular protection and facilitates monocyte- and macrophage-dependent vascular remodeling. PirB is an alternate receptor for Nogo-B, but a role for the Nogo-PirB axis within the vascular system has not been previously reported. We examined whether Nogo-B or PirB play a role in regulating macrophage-mediated vascular remodeling and hypothesized that endothelial Nogo-B regulates vein graft macrophage infiltration via its alternate receptor PirB. METHODS: Vein grafts were performed using Nogo and PirB wild type and knockout mice. Human vein grafts were similarly analyzed. The hindlimb ischemia model was performed in PirB wild type and knockout mice. Accompanying in vitro work included isolation of macrophages from PirB wild type and knockout mice. RESULTS: Increased Nogo-B and PirB mRNA transcripts and protein expression were observed within mouse and human vein grafts. Both Nogo knockout and PirB knockout vein grafts showed increased wall thickness and increased numbers of F4/80-positive macrophages. Macrophages derived from PirB knockout mice had increased adhesion to fibronectin, increased EC-specific binding, and increased numbers of mRNA transcripts of M2 markers as well as MMP3 and MMP9. PirB knockout vein grafts had increased active MMP9 compared to wild type vein grafts. PirB knockout mice had increased recovery from hindlimb ischemia and increased macrophage infiltration compared to wild type mice. CONCLUSIONS: Vein graft adaptation shows increased expression of both Nogo-B and PirB. Loss of PirB, or its endothelial ligand Nogo-B, results in increased inflammatory cell infiltration and vein graft wall thickening. These findings suggest that PirB regulates macrophage activity in vein grafts and that Nogo-B in the vein graft limits macrophage infiltration and vein graft thickening. PirB may play a more general role in regulating macrophage responses to vascular injury. Macrophage inhibition via Nogo-PirB interactions may be an important mechanism regulating vein graft adaptation to the arterial circulation.

Vascular endothelial growth factor-A inhibits EphB4 and stimulates delta-like ligand 4 expression in adult endothelial cells.

BACKGROUND: During vein graft adaptation to the arterial circulation, vascular endothelial growth factor (VEGF) A expression transiently increases before becoming downregulated; however, the role of VEGF-A in venous remodeling is not clear. In addition, although VEGF-A stimulates angiogenesis and determines arterial identity in nascent arterial endothelial cells (EC), the role of VEGF-A in regulating identity in adult venous EC is also not clear. MATERIALS AND METHODS: EC, wild type (EphB4+/+) or heterozygous knockout (EphB4+/-), were stimulated with VEGF-A (0-100 ng/mL) and examined with quantitative polymerase chain reaction and western blotting. RESULTS: VEGF-A (100 ng/mL) inhibited expression of EphB4 and stimulated expression of delta-like ligand 4 (dll4) but did not stimulate either notch or EphrinB2 expression in adult venous EC. Pretreatment with VEGF receptor 2-neutralizing antibody abolished VEGF-stimulated downregulation of EphB4 but not the upregulation of dll4. Pretreatment with PD98059 or wortmannin showed that VEGF-A downregulation of EphB4 and upregulation of dll4 are mitogen-activated protein kinase kinase and extracellular signal-regulated kinase dependent but phosphatidylinositol 3 kinase-Akt independent. Compared with VEGF-induced EphB4 downregulation and dll4 upregulation in control EC, reduced EphB4 signaling in EphB4+/- EC showed even further downregulation of EphB4 and upregulation of dll4. CONCLUSIONS: Despite the genetic programming of arterial and venous EC fate, VEGF-A can repress venous identity in adult venous EC without induction of arterial identity. These changes in adult EC in vitro recapitulate the changes in identity described during vein graft adaptation to the arterial environment in vivo.

Reduced adult endothelial cell EphB4 function promotes venous remodeling.

Reduced EphB4 expression is observed during vein graft adaptation and is associated with increased venous wall thickening. These findings suggest that EphB4 may mediate normal adult venous endothelial cell (EC) function and vein graft adaptation. We therefore tested the functional significance of EphB4 using EC with genetically reduced EphB4 signaling. EC were isolated from EphB4(+/+) and EphB4(+/-) mice. In vitro function was assessed through EC proliferation, migration, nitric oxide (NO) synthesis, and chemokine production. A mouse vein graft model was used to correlate in vitro findings with in vivo vein grafts. Smooth muscle cells (SMC) were subjected to proliferation and migration assays using EphB4(+/+) and EphB4(+/-) EC-conditioned medium. EphB4(+/-) EC exhibited diminished proliferation (P < 0.0001, n = 6), migration (P < 0.0001, n = 3), and NO production (P = 0.0012, n = 3). EphB4(+/-) EC had increased VEGF-A mRNA (P = 0.0006, n = 6) and protein (P = 0.0106, n = 3) as well as increased secretion of VEGF-A (P = 0.0010, n = 5), PDGF-BB (P < 0.0001, n = 6), and TGF-ß1 (P < 0.0001, n = 6). EphB4(+/-)-conditioned medium promoted SMC proliferation (P < 0.0001, n = 7) and migration (P = 0.0358, n = 3). Vein grafts and EphB4(+/-) EC showed similarity with regard to VEGF-A and eNOS mRNA and protein expression. In conclusion, reduced venous EC EphB4 function is associated with a proangiogenic and mitogenic phenotype. EphB4(+/-) EC have increased secretion of SMC mitogens and reduced NO production that correlate with the thickened neointima formed during vein graft adaptation. These findings suggest that EphB4 remains active in adult venous EC and that loss of EphB4 plays a role in vein graft adaptation.