AI-Enhanced evaluation of YouTube content on post-surgical incontinence following pelvic cancer treatment.

Background: Several pelvic area cancers exhibit high incidence rates, and their surgical treatment can result in adverse effects such as urinary and fecal incontinence, significantly impacting patients' quality of life. Post-surgery incontinence is a significant concern, with prevalence rates ranging from 25 to 45% for urinary incontinence and 9-68% for fecal incontinence. Cancer survivors are increasingly turning to YouTube as a platform to connect with others, yet caution is warranted as misinformation is prevalent. Objective: This study aims to evaluate the information quality in YouTube videos about post-surgical incontinence after pelvic area cancer surgery. Methods: A YouTube search for "Incontinence after cancer surgery" yielded 108 videos, which were subsequently analyzed. To evaluate these videos, several quality assessment tools were utilized, including DISCERN, GQS, JAMA, PEMAT, and MQ-VET. Statistical analyses, such as descriptive statistics and intercorrelation tests, were employed to assess various video attributes, including characteristics, popularity, educational value, quality, and reliability. Also, artificial intelligence techniques like PCA, t-SNE, and UMAP were used for data analysis. HeatMap and Hierarchical Clustering Dendrogram techniques validated the Machine Learning results. Results: The quality scales presented a high level of correlation one with each other (p < 0.01) and the Artificial Intelligence-based techniques presented clear clustering representations of the dataset samples, which were reinforced by the Heat Map and Hierarchical Clustering Dendrogram. Conclusions: YouTube videos on "Incontinence after Cancer Surgery" present a "High" quality across multiple scales. The use of AI tools, like PCA, t-SNE, and UMAP, is highlighted for clustering large health datasets, improving data visualization, pattern recognition, and complex healthcare analysis.

May-Thurner Syndrome: The Worst-Case Scenario.

May-Thurner syndrome (MTS) is caused by compression of the left common iliac vein by the right common iliac artery against the spinal column. It can range from asymptomatic or present with subtle and unspecific signs and symptoms and rarely exhibit severe complications such as pulmonary embolism (PE). The diagnosis is confirmed by typical imaging findings. Treatment may include conservative measures, anticoagulation, endovascular or even surgical options. We report the case of a 20-year-old female who presented with cardiac arrest caused by an acute massive PE. Further study showed partial thrombosis of the internal iliac veins resulting from MTS. She continued anticoagulation therapy with low-molecular-weight heparin and then switched to edoxaban with a good clinical outcome. She was also referred to Vascular Surgery to discuss the possibility of iliac vein stenting. Abdominopelvic vascular compression syndromes include a large spectrum of conditions, and they are rarely considered as an etiology for venous thromboembolism. The clinical presentation of PE varies with several triggering factors and atypical presentation is more common in nonmalignant causes. The combination of noninvasive and invasive imaging modalities might be beneficial to establish a definitive diagnosis. Nevertheless, invasive procedures are often restricted to doubtful cases or to guide endovascular procedures which is the current treatment of choice. There is little evidence using nonvitamin K oral anticoagulants, but there are some case reports detailing their successful use. This case aims to point out the need for a profound understanding of different causes of deep vein and pulmonary thromboembolism; common entities in our practice but with a variety of clinical presentations and potentially caused by rare underlying conditions. MTS can be the origin of serious and deadly complications, hence the importance of early recognition and treatment.

In vitro and in vivo evaluation of novel chromeno[2,3-d]pyrimidinones as therapeutic agents for triple negative breast cancer.

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, and the limited therapeutic options show poor efficacy in patients, associated to severe side effects and development of resistance. Considering that chromene-based scaffolds proved to be attractive candidates for cancer therapy, herein we prepared new chromeno[2,3-d]pyrimidinone derivatives by a simple two step procedure, starting from the reaction of cyanoacetamide and a salicylaldehyde. A cell viability screening in several breast cancer cell lines allowed to identify two promising compounds with IC50 values in the low micromolar range for TNBC cells. These chromenes inhibited cell proliferation, induced cell cycle arrest and triggered cell death through apoptosis. In vivo studies revealed a safe profile in invertebrate and vertebrate animal models and confirmed their capacity to inhibit tumor growth in the CAM model, inducing significant tumor regression after 4 days of treatment. The two compounds identified in this study are promising drug candidates for TNBC treatment and valuable hits for future optimization, using the versatile synthetic platform that was developed.

Assessing the Quality of YouTube's Incontinence Information after Cancer Surgery: An Innovative Graphical Analysis.

BACKGROUND: Prostate and colorectum cancers rank among the most common cancers, and incontinence is a significant postsurgical issue affecting the physical and psychological well-being of cancer survivors. Social media, particularly YouTube, has emerged as a vital source of health information. While YouTube offers valuable content, users must exercise caution due to potential misinformation. OBJECTIVE: This study aims to assess the quality of publicly available YouTube videos related to incontinence after pelvic cancer surgery. METHODS: A search on YouTube related to "Incontinence after cancer surgery" was performed, and 108 videos were analyzed. Multiple quality assessment tools (DISCERN, GQS, JAMA, PEMAT, and MQ-VET) and statistical analyses (descriptive statistics and intercorrelation tests) were used to evaluate the characteristics and popularity, educational value, quality, and reliability of these videos, relying on novel graphical representation techniques such as Sankey and Chord diagrams. RESULTS: Strong positive correlations were found among quality rating scales, emphasizing agreement. The performed graphical analysis reinforced the reliability and validity of quality assessments. CONCLUSIONS: This study found strong correlations among five quality scales, suggesting their effectiveness in assessing health information quality. The evaluation of YouTube videos consistently revealed "high" quality content. Considering the source is mandatory when assessing quality, healthcare and academic institutions are reliable sources. Caution is advised with ad-containing videos. Future research should focus on policy improvements and tools to aid patients in finding high-quality health content.

Smooth muscle tumours of the uterus: MR imaging malignant predictive features-a 12-year analysis in a referral hospital in Portugal.

PURPOSE: To evaluate the magnetic resonance imaging (MRI) features that may help distinguish leiomyosarcomas from atypical leiomyomas (those presenting hyperintensity on T2-W images equal or superior to 50% compared to the myometrium). MATERIALS AND METHODS: The authors conducted a retrospective single-centre study that included a total of 57 women diagnosed with smooth muscle tumour of the uterus, who were evaluated with pelvic MRI, between January 2009 and March 2020. All cases had a histologically proven diagnosis (31 Atypical Leiomyomas-ALM; 26 Leiomyosarcomas-LMS). The MRI features evaluated in this study included: age at presentation, dimension, contours, intra-tumoral haemorrhagic areas, T2-WI heterogeneity, T2-WI dark areas, flow voids, cyst areas, necrosis, restriction on diffusion-weighted imaging (DWI), apparent diffusion coefficient (ADC) values, signal intensity and heterogeneity after contrast administration in T1-WI, presence and location of unenhanced areas. The association between the MRI characteristics and the histological subtype was evaluated using Chi-Square and ANOVA tests. RESULTS: The MRI parameters that showed a statistically significance correlation with malignant histology and thus most strongly associated with LMS were found to be: irregular contours (p < 0.001), intra-tumoral haemorrhagic areas (p = 0.028), T2-WI dark areas (p = 0.016), high signal intensity after contrast administration (p = 0.005), necrosis (p = 0.001), central location for unenhanced areas (p = 0.026), and ADC value lower than 0.88 × 10-3 mm2/s (p = 0.002). CONCLUSION: With our work, we demonstrate the presence of seven MRI features that are statistically significant in differentiating between LMS and ALM.

Common mental disorders and risk perception in nursing work at covid-19 hospital units / Trastornos mentales comunes y percepción del riesgo en el trabajo de enfermería en unidades hospitalarias exclusivas para covid-19 / Transtornos mentais comuns e percepção de risco no trabalho de enfermagem em unidades hospitalares covid-19

ABSTRACT Objective: to analyze the associations between suspected Common Mental Disorders and risk perception in Nursing work at Covid-19 hospital units and to understand the elements intertwined in these relationships, from the workers' perspective. Method: a parallel-convergent mixed-methods study, carried out with 327 Nursing workers from Covid-19 units in seven hospitals from the state of Rio Grande do Sul, Brazil. The quantitative data were collected through a sociodemographic, work-related and risk perception questionnaire, added to the Self-Reporting Questionnaire and subjected to inferential statistical analysis. The qualitative data were produced through semi-structured interviews and treated in the light of thematic content analysis. Results: in all, 76.4% of the participants reported that their performance during the Covid-19 pandemic considerably or greatly increased the risks in their job. Regarding the risk exposure intensity, the workers consider themselves very exposed to the following risks: Covid-19 infection (51.4%); contaminating their family (45.9%); a family member developing a severe form of Covid-19 (46.5%); suffering psychological harms (47.7%); experiencing sleep (45.9%) or dietary pattern (40.7%) disorders; and isolating themselves from friends and family (48%). All of these variables were associated with suspected Common Mental Disorders. Qualitatively, it was evident that fear was the feeling signaling the intersection between risk perception and mental illness. Conclusion: suspected Common Mental Disorders were associated with the perception of multiple risks, with fear as the main element intertwined in this relationship.

RESUMEN Objetivo: analizar las asociaciones entre sospecha de Trastornos Mentales Comunes y percepción del riesgo en el trabajo de Enfermería en unidades hospitalarias exclusivas para COVID-19 y conocer los elementos entrelazados en estas relaciones, desde la perspectiva de los trabajadores. Método: estudio de métodos mixtos paralelo-convergente, realizado con 327 trabajadores da Enfermería de unidades exclusivas para COVID-19 de siete hospitales del estado de Rio Grande do Sul, Brasil. Los datos cuantitativos se recolectaron por medio de un cuestionario sociodemográfico, laboral y de percepción de riesgos, además de utilizarse el Self-Reporting Questionnaire, y se los sometió a análisis estadístico inferencial. Los datos cualitativos se produjeron por medio de entrevistas semiestructuradas y se los procesó sobre la base de análisis temático de contenido. Resultados: el 76,4% indicó que desempeñarse en la atención a pacientes con Covid-19 aumentó considerablemente o en demasía los riesgos en el trabajo. Con respecto a la intensidad de exposición a los riesgos, los trabajadores consideran estar muy expuestos a los siguientes riesgos: infección por Covid-19 (51,4%); contaminar a su familia (45,9%); que algún familiar desarrolle la forma grave da Covid-19 (46,5%); padecer perjuicios psicológicos (47,7%); sufrir alteraciones en el sueño (45,9) o en los hábitos alimenticios (40,7); u aislarse de familiares y amigos (48%). Todas estas variables demostraron estar asociadas a la sospecha de Trastornos Mentales Comunes. Cualitativamente, se evidenció que el sentimiento que señalizó la intersección entre percepción de riesgo y padecimiento mental fue el miedo. Conclusión: la sospecha de Trastornos Mentales Comunes se asoció a la percepción de múltiples riesgos, donde el miedo fue el principal elemento entrelazado en esa relación.

RESUMO Objetivo: analisar as associações entre a suspeita de Transtornos Mentais Comuns e a percepção de risco no trabalho da enfermagem em unidades hospitalares Covid-19 e conhecer os elementos imbricados nestas relações, na perspectiva dos trabalhadores. Método: estudo de métodos mistos paralelo-convergente, realizado com 327 trabalhadores da enfermagem de unidades Covid-19 de sete hospitais do estado do Rio Grande do Sul, Brasil. Os dados quantitativos foram coletados por meio de um questionário sociodemográfico, laboral e de percepção de riscos, somado ao Self-Reporting Questionnaire e submetidos a analise estatística inferencial. Os dados qualitativos foram produzidos por meio de entrevistas semiestruturadas e tratados à luz da análise temática de conteúdo. Resultados: 76,4% referiram que a atuação na Covid-19 aumentou consideravelmente ou muito os riscos no seu trabalho. No que tange à intensidade de exposição dos riscos, trabalhadores se consideram muito expostos ao risco de infecção por Covid-19 (51,4%); risco de levar a contaminação para sua família (45,9%); risco de que alguém da família desenvolva a forma grave da Covid-19 (46,5%); risco de sofrer danos psicológicos (47,7%); risco de sofrer alterações do sono (45,9); do padrão alimentar (40,7); e de se isolar de amigos e familiares (48%). Todas estas variáveis se mostraram associadas a suspeita de Transtornos Mentais Comuns. Qualitativamente, evidenciou-se que o medo era o sentimento balizador da intersecção entre a percepção de risco e o adoecimento mental. Conclusão: a suspeita de Transtornos Mentais Comuns associou-se à percepção de múltiplos riscos, sendo que o medo foi o principal elemento imbricado nessa relação.

Generalized Resistance Deficits in inmates with hypertension: missing resources that limit health / Déficits de Resistencia Generalizados en reclusos con hipertensión: recursos faltantes que limitan la salud / Déficits Generalizados de Resistência em pessoas presas com hipertensão: recursos ausentes que limitam a saúde

ABSTRACT Objective: to understand the Generalized Resistance Deficits of people deprived of liberty with hypertension in a Brazilian prison unit. Method: qualitative research, anchored in Salutogenesis, carried out with 38 people with hypertension from a Brazilian prison unit, from February to July 2022, with a semi-structured interview with open-ended questions, whose analysis was thematic, explaining the limitations to health in prison. Results: 13 Generalized Resistance Deficits were reported, mostly related to the prison environment and, to a lesser extent, to the social group and the individual, respectively. Living in prison for people with hypertension implies living with a high number of Generalized Resistance Deficits, accentuating the movement towards the disease pole. Final considerations: knowing Generalized Resistance Deficits allows directing health promotion to support the use of available Generalized Resistance Resources and contributes to the expansion of intersectoral policies.

RESUMEN Objetivo: comprender los Déficits de Resistencia Generalizados de personas privadas de libertad con hipertensión arterial sistémica en una unidad penitenciaria brasileña. Método: investigación cualitativa, anclada en la Salutogénesis, realizada con 38 personas con hipertensión arterial sistémica de una unidad penitenciaria brasileña, de febrero a julio de 2022, con entrevista semiestructurada con preguntas abiertas, cuyo análisis fue temático, explicando las limitaciones a la salud en prisión. Resultados: se reportaron 13 Déficits de Resistencia Generalizados, en su mayoría relacionados con el ambiente penitenciario y, en menor medida, con el grupo social y el individuo, respectivamente. Vivir en prisión para personas con hipertensión arterial sistémica implica vivir con un elevado número de Déficits de Resistencia Generalizados, acentuando el movimiento hacia el polo de la enfermedad. Consideraciones finales: conocer los Déficits de Resistencia Generalizada permite orientar la promoción de la salud para apoyar el uso de los Recursos de Resistencia Generalizada disponibles y contribuye a la ampliación de políticas intersectoriales.

RESUMO Objetivo: apreender os Déficits Generalizados de Resistência de pessoas privadas de liberdade com hipertensão arterial sistêmica de uma unidade prisional brasileira. Método: pesquisa qualitativa, ancorada na Salutogênese, realizada com 38 pessoas com hipertensão arterial sistêmica de uma unidade prisional brasileira, de fevereiro a julho de 2022, com entrevista semiestruturada de questões abertas, cuja análise foi temática, explicitando os limitantes à saúde na prisão. Resultados: foram relatados 13 Déficits Generalizados de Resistência, relacionando-se em maioria ao ambiente prisional e, em menor proporção, ao grupo social e ao indivíduo, respectivamente. O viver na prisão para pessoas com hipertensão arterial sistêmica implica conviver com elevado número de Déficits Generalizados de Resistência, acentuando o movimento em direção ao polo doença. Considerações finais: conhecer os Déficits Generalizados de Resistência permite direcionar a promoção da saúde para apoiar o uso dos Recursos Generalizados de Resistência disponíveis e contribui com a ampliação de políticas intersetoriais.

Sociodemographic, clinical and survival profile of adult metastatic patients.

OBJECTIVE: To characterize the sociodemographic, clinical and survival profile of adult metastatic patients. METHOD: Retrospective cross-sectional study, with secondary data from an oncology care unit, analyzed using logistic regression, Chi-Square test and Fisher's exact test, Kaplan-Meier and Log-Rank tests. RESULTS: From the 678 patients, male gender, mean age 59.54 years old and low education level prevailed. The mean time between diagnosis and initiation of treatment was 89.50 days (± 58.87). Increased risk of primary cancer in the digestive tract (OR 1.42). Prevalence of adenocarcinoma (OR 1.53) and metastasis to bone (OR 2.59), lymph nodes (OR 1.75), liver and peritoneum (OR 1.42). The mean overall survival was 4.16 months and a median of 3.0 months. CONCLUSION: The main primary site was the digestive system, and the identification of metastases was predominantly unifocal liver in both genders. Overall patient survival was reduced by cancer progression.

Efectividad de las intervenciones contra la violencia laboral que sufren los profesionales de la salud y de apoyo: metanálisis / Physical and psychological repercussions on Nursing workers' health in COVID-19 units: A mixed-methods research study / Repercussões físicas e psicológicas na saúde de trabalhadores de enfermagem em unidades COVID-19: pesquisa de métodos mistos

Objetivo: analizar cómo los trabajadores de enfermería de unidades hospitalarias de COVID-19 perciben las repercusiones físicas y psicológicas del trabajo sobre su salud, e identificar los factores asociados a la percepción. Método: estudio de métodos mixtos de convergencia paralela realizado con 359 trabajadores de enfermería de unidades de COVID-19 en siete hospitales. Para la recolección de datos cuantitativos se utilizó un cuestionario que contiene variables sociodemográficas, laborales y relacionadas con la percepción de repercusiones físicas y psicológicas, y para los datos cualitativos se utilizaron entrevistas semiestructuradas. Para el análisis se utilizó estadística inferencial y análisis de contenido temático Resultados: os trabajadores diurnos, que tenían más de un trabajo y trabajaban más de 41 horas/semana, percibieron las repercusiones físicas como más moderadas/intensas, debido a la sobrecarga y falta de días libres. Los enfermeros CLT percibieron repercusiones psicológicas más moderadas/intensas, y las atribuyeron a la sobrecarga gerencial y la insatisfacción laboral. Las mujeres tenían 97% más probabilidades de percibir repercusiones físicas y tres veces más probabilidades de percibir repercusiones psicológicas, en comparación con los hombres, en referencia a la sobrecarga doméstica y familiar. Conclusión: las sobrecargas laborales y familiares, intensificadas por la pandemia, se asociaron con la intensidad con la que los trabajadores de enfermería percibieron las repercusiones físicas y psíquicas.

Objective: to analyze how Nursing workers in COVID-19 hospital units perceive the physical and psychological repercussions of work on their health, as well as to identify the factors associated with their perceptions. Method: a parallel-convergent mixedmethods study conducted with 359 Nursing workers from COVID-19 units in seven hospitals. For the collection of quantitative data, a questionnaire containing sociodemographic and labor variables and related to perceptions of physical and psychological repercussions were used, and for qualitative data, semi-structured interviews were used. For the analysis, inferential statistics and thematic content analysis were used. Results: daytime workers, who had more than one employment contract and worked more than 41 hours/week perceived more moderate/intense physical repercussions, reporting overload and time off deficits. Nurses and CLT workers perceived psychological repercussions more moderately/intensely, mentioning managerial overload and job dissatisfaction. Women were 97% more likely to perceive physical repercussions and three times more likely to perceive psychological repercussions when compared to men, reporting household and family overloads. Conclusion: work and family overloads, intensified by the pandemic context, were associated with the intensity with which Nursing workers perceived physical and psychological repercussions.

Objetivo: analisar como os trabalhadores de enfermagem de unidades hospitalares COVID-19 percebem as repercussões físicas e psicológicas do trabalho em sua saúde e os fatores associados à sua percepção. Método: estudo de métodos mistos paralelo-convergente realizado com 359 trabalhadores lotados em unidades COVID-19 de sete hospitais. Para a coleta de dados quantitativos foram utilizados um questionário contendo variáveis sociodemográficas, laborais e relacionadas às percepções de repercussões físicas e psicológicas e, para a qualitativa, entrevistas semiestruturadas. Para a análise utilizou-se estatística inferencial e análise temática de conteúdo. Resultados: trabalhadores diurnos, que tinham mais de um vínculo empregatício e trabalhavam mais de 41 horas/semana perceberam as repercussões físicas como mais moderadas/intensas, devido à sobrecarga e déficit de folgas. Enfermeiros e celetistas perceberam as repercussões psicológicas mais moderadas/intensas, atribuindo-as à sobrecarga gerencial e insatisfação trabalhista. Mulheres apresentaram 97% mais chance de perceberem repercussões físicas e três vezes mais chance de perceberem repercussões psicológicas, quando comparadas aos homens, referindo sobrecarga doméstica e familiar. Conclusão: sobrecargas laborais e familiares, intensificadas pelo contexto pandêmico, foram associadas à intensidade com a qual os trabalhadores da enfermagem perceberam repercussões físicas e psicológicas.

Envelhecimento cutâneo: interlocuções entre o cuidado de enfermagem e a corporeidade em Merleau-Ponty / Skin aging: interlocutions between nursing care and corporeity in Merleau-Ponty / Envejecimiento de la piel: interlocuciones entre el cuidado de enfermería y la corporeidad en Merleau-Ponty

Objetivo: refletir acerca das possíveis interlocuções entre o cuidado de enfermagem e a corporeidade no tocante ao envelhecimento cutâneo à luz de Merleau-Ponty. Conteúdo: estudo teórico-reflexivo fundamentado na fenomenologia de Merleau-Ponty, com ênfase na obra Fenomenologia da Percepção e ao cuidado de enfermagem acerca de pessoas idosas que vivenciam o envelhecimento cutâneo. Organiza-se o exposto pelas categorias "O fenômeno da corporeidade na pessoa idosa à luz de Merleau-Ponty" e "Interlocuções entre Merleau-Ponty e o cuidado de enfermagem sobre o processo de envelhecimento cutâneo". Considerações finais: ao conceber as interlocuções, compreende-se que a objetivação do corpo vislumbra a intencionalidade de retorno ao corpo físico jovial e ao considerar o autoconhecimento, valorização do seu corpo e a vivência da corporeidade é possível uma melhor compreensão de si e do outro por meio da intersubjetividade que contribui com o desvelar de modificações no próprio ser(AU)

Objective: to reflect on the possible interlocutions between nursing care and corporeality regarding skin aging in the light of Merleau-Ponty. Content: theoretical-reflective study based on Merleau-Ponty's phenomenology, with emphasis on the work Phenomenology of Perception and nursing care about elderly people who experience skin aging. The above is organized into the categories "The phenomenon of embodiment in the elderly in the light of Merleau-Ponty" and "Interlocutions between Merleau-Ponty and nursing care on the skin aging process". Final considerations: when conceiving the interlocutions, it is understood that the objectification of the body envisions the intentionality of returning to the jovial physical body and when considering self-knowledge, appreciation of your body and the experience of corporeity, a better understanding of yourself and the other is possible through intersubjectivity that contributes to the unveiling of changes in the being itself(AU)

Objetivo: reflexionar sobre las posibles interlocuciones entre el cuidado de enfermería y la corporeidad en relación con el envejecimiento de la piel a la luz de Merleau-Ponty. Contenido: estudio teórico-reflexivo fundamentado en la fenomenología de Merleau-Ponty, con énfasis en la obra Fenomenología de la percepción y del cuidado de enfermería acerca de las personas mayores que experimentan el envejecimiento de la piel. Se organiza el estudio en las categorías "El fenómeno de la corporeidad en el anciano a la luz de Merleau-Ponty" e "Interlocuciones entre Merleau-Ponty y el cuidado de enfermería en el proceso de envejecimiento de la piel". Consideraciones finales: al concebir las interlocuciones se entiende que la objetivación del cuerpo vislumbra la intencionalidad de volver al cuerpo físico jovial y al considerar el autoconocimiento, la apreciación de su cuerpo y la experiencia de la corporeidad, una mejor comprensión de ti mismo y el otro a través de la intersubjetividad que contribuye al desvelamiento de cambios en el propio ser es posible(AU)

Fatores associados à punção venosa periférica difícil em adultos submetidos à quimioterapia antineoplásica / Factors associated with difficult peripheral venipuncture in adults undergoing antineoplastic chemotherapy / Factores asociados a la venopunción periférica difícil en adultos sometidos a quimioterapia antineoplásica

Objetivo: identificar os fatores associados à punção venosa periférica difícil em adultos submetidos à quimioterapia antineoplásica. Método: estudo transversal, observacional, analítico e quantitativo realizado em uma Unidade de Alta Complexidade em Oncologia (UNACON) da região amazônica brasileira. Os dados foram analisados por meio de estatística descritiva e inferencial, onde a razão de possibilidades foi calculada. Resultados: a maioria dos participantes foi do sexo feminino (64,6%), autodeclarados como pardos (51,2%). Em relação à localização do câncer, a maioria possuía a doença no aparelho digestório (46,4%) ou reprodutor (45,2%). Pacientes que tinham histórico de punção venosa difícil, veias não visíveis ou não palpáveis apresentaram mais chance de apresentar a punção venosa difícil (OR 1,6, 1,5 e 1,3, respetivamente). Conclusão: os preditores encontrados relacionados à punção venosa periférica difícil em pacientes adultos submetidos à quimioterapia antineoplásica foram: histórico de punção difícil e veias não visíveis ou não palpáveis(AU)

Objective: to identify factors associated with difficult peripheral venipuncture in adults undergoing antineoplastic chemotherapy. Method: cross-sectional, observational, analytical, and quantitative study carried out in a High Complexity Oncology Unit (UNACON) in the Brazilian Amazon region. Data were analyzed using descriptive and inferential statistics, where the odds ratio was calculated. Results: majority of participants were female (64.6%), self-declared as brown (51.2%). Regarding the location of the cancer, the majority had the disease in the digestive (46.4%) or reproductive (45.2%) systems. Patients who had a history of difficult venipuncture, non-visible or non-palpable veins were more likely to have difficult venipuncture (OR 1.6, 1.5 and 1.3, respectively). Conclusion: found predictors related to difficult peripheral venipuncture in adult patients undergoing antineoplastic chemotherapy were: history of difficult puncture and non-visible or non-palpable veins(AU)

Objetivo: identificar los factores asociados a la dificultad de la venopunción periférica en adultos sometidos a la quimioterapia antineoplásica. Método: estudio transversal, observacional, analítico y cuantitativo realizado en una Unidad de Oncología de Alta Complejidad (UNACON) en la Amazonía brasileña. Se analizaron los datos mediante estadística descriptiva e inferencial, donde se calculó el odds ratio. Resultados: la mayoría de los participantes era del sexo femenino (64,6%), se auto declaró morena (51,2%). En cuanto a la ubicación del cáncer, la mayoría tenía la enfermedad en el sistema digestivo (46,4%) o reproductivo (45,2%). Los pacientes que tenían antecedentes de venopunción difícil, venas no visibles o no palpables tenían más probabilidades de tener venopunción difícil (OR 1,6, 1,5 y 1,3, respectivamente). Conclusión: Los predictores encontrados relacionados con la punción venosa periférica difícil en pacientes adultos sometidos a quimioterapia antineoplásica fueron antecedente de punción difícil y venas no visibles o no palpables(AU)

Characterization of a cohort of metastatic lung cancer patients harboring KRAS mutations treated with immunotherapy: differences according to KRAS G12C vs. non-G12C.

Approximately 20% of lung adenocarcinomas harbor activating mutations at KRAS, an oncogene with the ability to alter the tumor immune microenvironment. In this retrospective study, we examined 103 patients with KRAS-mutant lung adenocarcinoma who were treated with immunotherapy-based regimens and we evaluated the clinical outcomes according to PD-L1 expression and the type of KRAS mutation. Among all patients included, 47% carried KRAS G12C mutation whereas 53% harbored KRAS non-G12C mutations. PD-L1 status was available for 77% of cases, with higher expression among KRAS G12C tumors (p = 0.01). Better overall survival and progression-free survival were observed in high PD-L1 expression tumors, regardless of KRAS mutation type. The heterogeneous nature of KRAS-mutant tumors and the presence of other co-mutations may contribute to different outcomes to immunotherapy-based strategies.

Breast Cancer: Clinical-Epidemiological Profile and Toxicities of Women Receiving Treatment with Taxanes in the Amazon Region.

Breast cancer is the most common malignant disease and the leading cause of mortality among women worldwide. Antineoplastic chemotherapy is one of its primary treatments, typically based on the class of drugs known as taxanes. Despite their proven therapeutic efficacy, these drugs can induce severe toxicities, leading to dose limitations or even treatment discontinuation. The objective of this study was to describe the clinical-epidemiological profile, risk factors, and toxicities of taxane-based chemotherapy treatment in women with breast cancer in the Amazon region. This is a cross-sectional, quantitative, and descriptive study conducted with 300 women diagnosed with breast cancer undergoing taxane treatment. Most patients were in the 40-49 age range, of brown ethnicity, and had completed elementary school. The majority of patients had risk factors such as alcoholism and a sedentary lifestyles. Most women had their first pregnancy between the ages of 18 and 21, breastfed their children, had menarche between the ages of 12 and 13, and were pre-menopausal and with a family history of cancer. The most frequent histological type was non-special invasive carcinoma and the Luminal B subtype. Most participants in this study showed taxane toxicity, with neurotoxicity being the most frequent. These findings reveal the importance of early detection, comprehensive risk factors, and effective management of treatment toxicities to improve patient outcomes in breast cancer care in the Amazon region.

Exosomes modified with anti-MEK1 siRNA lead to an effective silencing of triple negative breast cancer cells.

Triple negative breast cancer (TNBC) is a highly heterogenous disease not sensitive to endocrine or HER2 therapy and standardized treatment regimens are still missing. Therefore, development of novel TNBC treatment approaches is of utmost relevance. Herein, the potential of MAPK/ERK downregulation by RNAi-based therapeutics in a panel of mesenchymal stem-like TNBC cell lines was uncovered. Our data revealed that suppression of one of the central nodes of this signaling pathway, MEK1, affects proliferation, migration, and invasion of TNBC cells, that may be explained by the reversion of the epithelial-mesenchymal transition phenotype, which is facilitated by the MMP-2/MMP-9 downregulation. Moreover, an exosome-based system was successfully generated for the siRNA loading (iExoMEK1). Our data suggested absence of modification of the physical properties and general integrity of the iExoMEK1 comparatively to the unmodified counterparts. Such exosome-mediated downregulation of MEK1 led to a tumor regression accompanied by a decrease of angiogenesis using the chick chorioallantoic-membrane model. Our results highlight the potential of the targeting of MAPK/ERK cascade as a promising therapeutic approach against TNBC.

Cotidiano e desafios da enfermagem em unidades hospitalares COVID-19: perspectiva dos profissionais / Daily life and challenges of nursing in COVID-19 hospital units: professionals' perspective

Introdução: O trabalho de enfermagem em unidades hospitalares COVID-19 é complexo, desafiador e repleto de elementos cuja compreensão é importante para o campo da Gestão e Saúde no Trabalho. Objetivo: Compreender o cotidiano e os desafios de trabalhadores de enfermagem na linha de frente do enfrentamento à pandemia em unidades hospitalares COVID-19. Materiais e Métodos: Estudo qualitativo desenvolvido com 35 trabalhadores de enfermagem em unidades COVID-19 de sete hospitais do Sul do Brasil, por meio de entrevistas semiestruturadas. O software NVivo auxiliou no tratamento dos dados a partir da análise temática de conteúdo. Resultados: Da análise dos dados emergiram duas categorias analíticas: Desafios do cotidiano de enfermagem nas unidades COVID-19: complexidade e demandas da assistência; e Desafios de ser um trabalhador de enfermagem da linha de frente: desdobramentos no bem-estar profissional e na vida pessoal. Discussão: Pode-se considerar que a complexidade e intensificação do trabalho na linha de frente causou impactos que podem conduzir os trabalhadores de enfermagem ao adoecimento. São importantes ações de promoção à visibilidade profissional junto à sociedade para desmistificar a imagem romantizada sobre a profissão e problematizar a importância da enfermagem no enfrentamento da pandemia e os impactos sofridos por estes trabalhadores. Conclusão: Trabalhadores de enfermagem de unidades COVID-19 vivenciam desafios complexos, com repercussões em sua experiência laboral e em sua vida.

Introduction: The nursing work in COVID-19 hospital units is complex, challenging, and full of elements whose understanding is important for the field of Management and Health at Work. Objective: To understand the daily life and challenges of nursing workers in the front line of confronting the pandemic in COVID-19 hospital units. Materials and Methods: Qualitative study developed with 35 nursing workers in COVID-19 units from seven hospitals in southern Brazil, through semi-structured interviews. The NVivo software helped in the data treatment from the thematic content analysis. Results: Two analytical categories emerged from data analysis: Challenges of nursing daily life in COVID-19 units: complexity and demands of assistance; and Challenges of being a frontline nursing worker: unfolding in professional well-being and personal life. Discussion: It can be considered that the complexity and intensification of frontline work caused impacts that can lead nursing workers to illness. Actions to promote professional visibility in society are important, to demystify the romanticized image about the profession, and to problematize the importance of nursing in facing the pandemic and the impacts suffered by these workers. Conclusion: Nursing workers in COVID-19 units experience complex challenges, with repercussions on their work experience and their lives.

Introducción: El trabajo de enfermería en las unidades hospitalarias COVID-19 es complejo, desafiante y lleno de elementos cuya comprensión es importante para el campo de la Gestión y Salud Ocupacional. Objetivo: comprender el cotidiano y los desafíos de los trabajadores de enfermería en la primera línea de enfrentamiento a la pandemia en las unidades hospitalarias de la COVID-19. Materiales y Métodos: estudio cualitativo desarrollado con 35 trabajadores de enfermería en unidades de COVID-19 de siete hospitales del sur de Brasil, a través de entrevistas semiestructuradas. El software NVivo ayudó en el procesamiento de datos basado en el análisis de contenido temático. Resultados: del análisis de los datos surgieron dos categorías analíticas: Desafíos cotidianos de la enfermería en las unidades de COVID-19: complejidad y demandas asistenciales; y Desafíos de ser un trabajador de enfermería de primera línea: desarrollos en el bienestar profesional y personal. Discusión: se puede considerar que la complejidad e intensificación del trabajo en primera línea provocó impactos que podrían llevar a los trabajadores de enfermería a la enfermedad. Las acciones de promoción de la visibilidad profesional en la sociedad son importantes para desmitificar la imagen romantizada de la profesión y discutir la importancia de la enfermería en el enfrentamiento de la pandemia y los impactos sufridos por estos trabajadores. Conclusión: los trabajadores de enfermería de las unidades de COVID-19 viven desafíos complejos, con repercusiones en su experiencia laboral y en su vida.

Prognostic Value of Monocarboxylate Transporter 1 Overexpression in Cancer: A Systematic Review.

Energy production by cancer is driven by accelerated glycolysis, independently of oxygen levels, which results in increased lactate production. Lactate is shuttled to and from cancer cells via monocarboxylate transporters (MCTs). MCT1 works both as an importer and an extruder of lactate, being widely studied in recent years and generally associated with a cancer aggressiveness phenotype. The aim of this systematic review was to assess the prognostic value of MCT1 immunoexpression in different malignancies. Study collection was performed by searching nine different databases (PubMed, EMBASE, ScienceDirect, Scopus, Cochrane Library, Web of Science, OVID, TRIP and PsycINFO), using the keywords "cancer", "Monocarboxylate transporter 1", "SLC16A1" and "prognosis". Results showed that MCT1 is an indicator of poor prognosis and decreased survival for cancer patients in sixteen types of malignancies; associations between the transporter's overexpression and larger tumour sizes, higher disease stage/grade and metastasis occurrence were also frequently observed. Yet, MCT1 overexpression correlated with better outcomes in colorectal cancer, pancreatic ductal adenocarcinoma and non-small cell lung cancer patients. These results support the applicability of MCT1 as a biomarker of prognosis, although larger cohorts would be necessary to validate the overall role of MCT1 as an outcome predictor.

Enhanced bioprocess control to advance the manufacture of mesenchymal stromal cell-derived extracellular vesicles in stirred-tank bioreactors.

Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) act as signaling mediators of cellular responses. However, despite representing a promising alternative to cell-based therapies, clinical translation of EVs is currently limited by their lack of scalability and standardized bioprocessing. Herein, we integrated scalable downstream processing protocols with standardized expansion of large numbers of viable cells in stirred-tank bioreactors to improve EV production. Higher EV yields were linked to EV isolation by tangential flow filtration followed by size exclusion chromatography, rendering 5 times higher number of EVs comparatively to density gradient ultracentrifugation protocols. Additionally, when compared to static culture, EV manufacture in bioreactors resulted in 2.2 higher yields. Highlighting the role of operating under optimal cell culture conditions to maximize the number of EVs secreted per cell, MSCs cultured at lower glucose concentration favored EV secretion. While offline measurements of metabolites concentration can be performed, in this work, Raman spectroscopy was also applied to continuously track glucose levels in stirred-tank bioreactors, contributing to streamline the selection of optimal EV collection timepoints. Importantly, MSC-derived EVs retained their quality attributes and were able to stimulate angiogenesis in vitro, therefore highlighting their promising therapeutic potential.

Satisfação com os cuidados de enfermagem de pacientes oncológicos em uso de antineoplásicos ambulatorial / Satisfaction with nursing care of oncology patients on outpatient antineoplastic drugs / Satisfacción con los cuidados de enfermería en pacientes oncológicos que utilizan fármacos antineoplásicos en régimen ambulatorio

RESUMO Objetivo: avaliar a satisfação do usuário oncológico ambulatorial em uso de antineoplásicos sobre os cuidados de enfermagem. Métodos: estudo transversal com abordagem quantitativa, realizado no Serviço de Quimioterapia de um hospital universitário localizado em Belém-PA - Brasil, com 200 usuários no período de junho de 2019 a junho de 2022, através de entrevista e uso do Instrumento de Satisfação do Paciente. Aplicou-se análise de qui-quadrado de Pearson e considerou-se p-valor ≤0,05. Resultados: os usuários relataram bom índice de satisfação na média geral dos domínios (>90%), destacando o domínio técnico-profissional, seguido do educacional e confiança. As variáveis de esclarecimento sobre as orientações médicas, dar bons conselhos, tomar iniciativa após as respostas dos pacientes foram associadas à satisfação do usuário conforme a topografia do câncer e a disponibilidade da equipe àqueles com diferentes graus de estadiamento. Conclusão: este estudo auxilia gestores na identificação e planejamento de melhorias nas áreas e serviços.

ABSTRACT Objective: Evaluate the satisfaction of outpatient oncology users taking antineoplastic drugs about nursing care. Methods: Exsectional study with a quantitative approach, carried out at the Chemotherapy Service of a university hospital located in Belém-PA - Brazil, with 200 users, from June 2019 to June 2022, through interviews and use of the Patient Satisfaction Instrument. Chi-square analysis, Pearson p-value, and chi-square analysis were applied, and the p-value ≤ 0.05 was considered. Results: Users reported good satisfaction in the overall average of the domains (>90%), highlighting the technical-professional domain, followed by the educational and confidence domains. The variables of clarification of medical guidelines, giving good advice, and taking the initiative after patient responses were associated with user satisfaction according to cancer topography and staff availability to those with different degrees of staging. Conclusion: This study assists managers in identifying and planning improvements in areas and services.

RESUMEN Objetivo: evaluar la satisfacción de los usuarios de oncología ambulatoria que utilizan fármacos antineoplásicos sobre los cuidados de enfermería. Métodos: estudio seccional con enfoque cuantitativo, realizado en el Servicio de Quimioterapia de un hospital universitario situado en Belém-PA - Brasilcon 200 usuarios en el período de junio de 2019 a junio de 2022, mediante entrevista y utilización del Instrumento de Satisfacción del Paciente. Se aplicó el análisis chi-cuadrado de Pearson y el valor p ≤0,05. Resultados: los usuarios declararon una buena satisfacción en la media global de los dominios (>90%), destacando el dominio técnico-profesional, seguido de los dominios educativo y de confianza. Las variables de aclaración sobre las directrices médicas, dar buenos consejos, tomar la iniciativa tras las respuestas de los pacientes se asociaron con la satisfacción de los usuarios según la topografía del cáncer y la disponibilidad del personal ante los distintos grados de estadificación. Conclusión: este estudio ayuda a los directivos a identificar y planificar mejoras en áreas y servicios.

Glucose Metabolism Reprogramming in Bladder Cancer: Hexokinase 2 (HK2) as Prognostic Biomarker and Target for Bladder Cancer Therapy.

Proliferating cancer cells are able to reprogram their energy metabolism, favouring glycolysis even in the presence of oxygen and fully functioning mitochondria. Research is needed to validate the glycolysis-related proteins as prognostic/predictive biomarkers in urothelial bladder carcinoma (UBC), a malignancy tagged by high recurrence rates and poor response to chemotherapy. Here, we assessed GLUT1, HK2, PFKL, PKM2, phospho-PDH, and LDHA immunoexpression in 76 UBC samples, differentiating among urothelial, fibroblast, and endothelial cells and among normoxic versus hypoxic areas. We additionally studied the functional effects of the HK2 inhibitor 2-deoxy-D-glucose (2DG) in "in vitro" and "in vivo" preclinical UBC models. We showed that the expression of the glycolysis-related proteins is associated with UBC aggressiveness and poor prognosis. HK2 remained as an independent prognostic factor for disease-free and overall survival. 2DG decreased the UBC cell's viability, proliferation, migration, and invasion; the inhibition of cell cycle progression and apoptosis occurrence was also verified. A significant reduction in tumour growth and blood vessel formation upon 2DG treatment was observed in the chick chorioallantoic membrane assay. 2DG potentiated the cisplatin-induced inhibition of cell viability in a cisplatin-resistant subline. This study highlights HK2 as a prognostic biomarker for UBC patients and demonstrates the potential benefits of using 2DG as a glycolysis inhibitor. Future studies should focus on integrating 2DG into chemotherapy design, as an attempt to overcome cisplatin resistance.