Chemical characterisation, thermal analysis, and antibacterial activity of honeys from Caatinga stingless bees of Melipona spp.

In the present study, chemical characterisation, thermal analysis and antibacterial activity of honeys from Melipona spp. with occurrence in Caatinga biome of Brazil. The honeys presented pH from 4.07 to 4.14, density of 1.41 g/cm3 and °Brix value of 79.90. The thermogravimetry (TG) analysis presented six-seven events and differential thermal analysis (DTA) presented three-four endothermic peaks. HPLC fingerprint revealed a predominant presence of gallic acid and vanillin. Antioxidant activity evaluated using in vitro 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) radical scavenging with IC50 values in the range of 14.5404 to 15.2454 mg/mL. The honeys also showed antimicrobial activities against Staphylococcus aureus and Pseudomonas aeruginosa using a modified agar diffusion and microdilution method. The results of the present study demonstrate that the honey from stingless bees by Caatinga biome indicate polyphenol compounds, antioxidant activity and in vitro antimicrobial potential. The analytical methos permitted of fingerprint of samples.

Unveiling the RKIP and EGFR Inverse Relationship in Solid Tumors: A Case Study in Cervical Cancer.

Raf Kinase Inhibitor Protein (RKIP) is recognized as a bona fide tumor suppressor gene, and its diminished expression or loss is associated with the progression and poor prognosis of various solid tumors. It exerts multifaceted roles in carcinogenesis by modulating diverse intracellular signaling pathways, including those governed by HER receptors such as MAPK. Given the significance of HER receptor overexpression in numerous tumor types, we investigated the potential oncogenic relationship between RKIP and HER receptors in solid tumors. Through a comprehensive in silico analysis of 30 TCGA PanCancer Atlas studies encompassing solid tumors (10,719 samples), we uncovered compelling evidence of an inverse correlation between RKIP and EGFR expression in solid tumors observed in 25 out of 30 studies. Conversely, a predominantly positive association was noted for the other HER receptors (ERBB2, ERBB3, and ERBB4). In particular, cervical cancer (CC) emerged as a tumor type exhibiting a robust inverse association between RKIP and EGFR expression, a finding that was further validated in a cohort of 202 patient samples. Subsequent in vitro experiments involving pharmacological and genetic modulation of EGFR and RKIP showed that RKIP depletion led to significant upregulation of EGFR mRNA levels and induction of EGFR phosphorylation. Conversely, EGFR overactivation decreased RKIP expression in CC cell lines. Additionally, we identified a common molecular signature among patients depicting low RKIP and high EGFR expression and demonstrated the prognostic value of this inverse correlation in CC patients. In conclusion, our findings reveal an inverse association between RKIP and EGFR expression across various solid tumors, shedding new light on the underlying molecular mechanisms contributing to the aggressive phenotype associated with RKIP and EGFR in cervical cancer.

Shape-Versatile Fixed Cellular Materials for Multiple Target Immunomodulation.

Therapeutic cells are usually administered as living agents, despite the risks of undesired cell migration and acquisition of unpredictable phenotypes. Additionally, most cell-based therapies rely on the administration of single cells, often associated with rapid in vivo clearance. 3D cellular materials may be useful to prolong the effect of cellular therapies and offer the possibility of creating structural volumetric constructs. Here, the manufacturing of shape-versatile fixed cell-based materials with immunomodulatory properties is reported. Living cell aggregates with different shapes (spheres and centimeter-long fibers) are fixed using a method compatible with maintenance of structural integrity, robustness, and flexibility of 3D constructs. The biological properties of living cells can be modulated before fixation, rendering an in vitro anti-inflammatory effect toward human macrophages, in line with a decreased activation of the nuclear factor kappa B (NF-κB) pathway that preponderantly correlated with the surface area of the materials. These findings are further corroborated in vivo in mouse skin wounds. Contact with fixed materials also reduces the proliferation of activated primary T lymphocytes, while promoting regulatory populations. The fixation of cellular constructs is proposed as a versatile phenotypic stabilization method that can be easily implemented to prepare immunomodulatory materials with therapeutic potential.

Transepithelial Accelerated Crosslinking for Progressive Keratoconus: A Critical Analysis of Medium-Term Treatment Outcomes.

Purpose: To report the 4-year outcomes of transepithelial accelerated corneal collagen crosslinking (TE-ACXL) in the treatment of eyes with progressive keratoconus (KC). Methods: Eyes of patients who underwent TE-ACXL (6mW/cm2 for 15 minutes) for progressive KC and presented 48 months of follow-up were included. Corrected distance visual acuity (CDVA), keratometry measurements (Kmax, maximum keratometry, Kmean, mean keratometry and Astg, corneal astigmatism), thinnest corneal thickness (PachyMin), and topographic, and tomographic indices (specifically the posterior radius of curvature from the 3.0 mm centered on the thinnest point of the cornea (PRC), and the D-index) were analysed preoperatively and every 12 months after TE-ACXL, up to 48 months. Progression after TE-ACXL was considered when eyes presented ≥1 criteria: (1) increase of ≥1D in Kmax or increase of ≥0.75D in Kmean or increase of ≥1D in Astg; (2) reduction of ≥0.085 mm in PRC; (3) decrease ≥5% in PachyMin. Results: 41 eyes from 30 patients were included, with a mean age at crosslinking of 20.90±4.69 years. There was a significant increase in Kmean (+0.64±1.04 D, p<0.001; +0.98 ± 1.49 D, p<0.001; +1.27±2.01 D, p<0.001; +1.13±2.00 D, p=0.006) and a significant decrease in PRC throughout follow-up (-0.12±0.22, p=0.002; -0.15±0.24, p<0.001; -0.17±0.43, p=0.021; -0.16±0.43, p=0.027). PachyMin decreased significantly at 36 and 48 months (-8.50±15.93 µm, p=0.004; -7.82±18.37, p=0.033). According to our progression criteria, there was a major progression rate throughout follow-up (57.1%, 61.1%, 58.8%, and 67.9%, respectively). Surgery and follow-up were uneventful in all subjects. Eleven eyes (26.8%) required further procedures, ≥36 months after the initial TE-ACXL, due to persistent progressive disease. Conclusion: TE-ACXL proved to be a safe therapeutic option for progressive KC. However, its efficacy is deemed unsatisfactory, as a notable proportion of affected eyes may continue to advance within a 4-year timeframe, necessitating additional procedures to halt the disease's course.

Early observations with an ERAS pathway for thyroid and parathyroid surgery: Moving the goalposts forward.

BACKGROUND: Enhanced recovery after surgery pathways have become the standard of care in various surgical specialties. In this study, we discuss our initial experience with a staged enhanced recovery after surgery pathway in endocrine surgery and assess the impact of this pathway on select perioperative outcomes and unanticipated admissions. METHODS: We collected information regarding all thyroid/parathyroid surgeries performed by endocrine surgeons at our institution before and after the implementation of the multi-intervention enhanced recovery after surgery pathway. We compared relevant outcomes for all cases 1 year before (n = 479) and 1 year after (n = 166) implementation of the pathway. We also compared outcomes between enhanced recovery after surgery patient groups with varying levels of enhanced recovery after surgery compliance. RESULTS: Enhanced recovery after surgery was associated with a significant decrease in total length of stay (9.2 vs 7.5 hours, P < .0001). Whereas there was no significant decrease in all-cause unanticipated postoperative admissions, there was a decrease in patient-initiated admissions in the Enhanced recovery after surgery group. There was also a significant decrease in mean postoperative morphine milligram equivalents (14.4 vs 16.2 vs 24.8, P = .0015), average daily morphine milligram equivalents (25.6 vs 45.6 vs 53, P < .0001), and average daily pain scores (1.89 vs 2.38 vs 2.74, P = .0045) in the Enhanced recovery after surgery group (particularly with increasing Enhanced recovery after surgery compliance). There were no significant differences in the requirement for postoperative antiemetics or in the post-anesthesia care unit length of stay. CONCLUSION: This study demonstrates a significant benefit from Enhanced recovery after surgery pathways for thyroidectomies and parathyroidectomies, even with initial data and a staggered roll-out plan. Further directions include a follow-up study once we reach a higher level of institutional compliance with all components of the Enhanced Recovery After Surgery pathway and a prospective trial to identify the relative significance of different portions of the Enhanced Recovery after Surgery pathway, particularly the superficial cervical plexus block.

Autoinflammatory diseases: a Latin American multicenter study according to age and sex / Doenças autoinflamatórias: um estudo multicêntrico latino-americano de acordo com o sexo e a idade

ABSTRACT Objective: To evaluate autoinflammatory diseases (AID) according to age at diagnosis and sex, and response to therapy in a large population. Methods: This is a cross-sectional observational study of a Latin American registry using a designed web system for data storage, collected between 2015 and 2018. Any altered findings during follow-up were recorded. The forms were translated into Portuguese and Spanish, including demographic, clinical, laboratory, genetic and treatment characteristics. Results: We included 152 patients, 51.3% male and 75% Caucasian. The median age at disease onset was 2.1 years (0-15.6 years) and median age at diagnosis 6.9 years (0-21.9 years); 111 (73%) were children (0-9 years old), and 41 (27%) were adolescents and young adults (AYA) (10-21 years old). Periodic fever, aphthous stomatitis, pharyngitis, and adenitis syndrome (PFAPA) occurred in 46/152 (30%), chronic non-bacterial osteomyelitis (CNO) in 32/152 (21%), and familial Mediterranean fever (FMF) in 24/152 (15.7%). PFAPA was significantly higher in young children than in AYA (38.7% vs. 7.3%, p<0.001), while CNO were lower (13.5% vs. 41.5%, p<0.001). The frequency of females was significantly higher in CNO (28.4% vs. 14.1%, p=0.031) and lower in FMF (8.1% vs. 23.1%, p=0.011). The most used drugs were glucocorticoids, non-steroidal anti-inflammatory drugs (NSAID), and colchicine. Glucocorticoids and colchicine treatment were used in all AID with good to moderate response. However, cryopyrin-associated periodic syndromes (CAPS) seemed unresponsive to glucocorticoids. NSAIDs and methotrexate were the main medications used to treat CNO. Conclusions: Differences among AID patients were observed in the LA population regarding sex and age at disease diagnosis.

RESUMO Objetivo: Avaliar as doenças autoinflamatórias (DAI) de acordo com sexo e idade no momento do diagnóstico e a resposta terapêutica em uma grande população. Métodos: Este é um estudo observacional transversal de um registro latino-americano que usou um sistema de dados coletados entre 2015 e 2018. Quaisquer achados alterados ao longo do acompanhamento foram registrados. Os formulários foram traduzidos para os idiomas português e espanhol, incluindo características demográficas, clínicas, laboratoriais, genéticas e de tratamento. Resultados: Incluímos 152 pacientes, sendo 51,3% do sexo masculino e 75% da raça branca. A média de idade de início da doença foi de 2,1 anos (0-15,6 anos) e a média de idade de diagnóstico 6,9 anos (0-21,9 anos); 111 (73%) eram crianças (0-9 anos) e 41 (27%) adolescentes/adultos jovens (10-21 anos). A síndrome de febre periódica, estomatite aftosa, faringite e adenite (PFAPA) ocorreu em 46/152 (30%), osteomielite não bacteriana crônica (CNO) em 32/152 (21%) e febre familiar do Mediterrâneo (FMF) em 24/152 (15,7%). A PFAPA foi significativamente maior em crianças pequenas (38,7 vs. 7,3%, p<0,001), e a CNO, em adolescentes/adultos jovens (13,5 vs. 41,5%, p<0,001). A frequência do sexo feminino foi significativamente maior na CNO (28,4 vs. 14,1%, p=0,031) e menor na FMF (8,1 vs. 23,1%, p=0,011). Os medicamentos mais utilizados foram glicocorticoides, anti-inflamatórios não esteroidais (AINE) e colchicina. O tratamento com glicocorticoides e colchicina foi usado em todas as DAI com resposta boa a moderada. No entanto, as síndromes periódicas associadas à criopirina (CAPS) pareciam não responder aos glicocorticoides. AINE e metotrexato foram os principais medicamentos utilizados no tratamento da CNO. Conclusões: Diferenças de pacientes com DAI foram observadas na população latino-americana em pacientes agrupados por sexo e idade ao diagnóstico da doença.

Terapia Ocupacional Social e Paulo Freire: Uma Revisão de escopo / Terapia Ocupacional Social y Paulo Freire: una revisión de alcance / Social Occupational Therapy and Paulo Freire: a scoping review

Paulo Freire é um importante autor que sustenta a práxis da Terapia Ocupacional Social. O intuito do presente trabalho é ampliar o escopo e adensar o caráter da presença acadêmica deste autor no campo da Terapia Ocupacional Social, tomando como referência produções científicas brasileiras. Como percurso metodológico, foi realizada uma revisão de escopo, com levantamento de estudos publicados em quatro periódicos brasileiros e bases de dados internacionais. As buscas foram realizadas utilizando a língua portuguesa. A revisão não definiu período inicial e considerou os artigos disponíveis online até fevereiro de 2023. Foram incluídos 18 estudos que possuem as obras de Freire em suas referências. Pedagogia do oprimido e Educação como prática de liberdade são as obras que mais aparecem nos artigos encontrados. Foi rea-lizado o refinamento das categorias freireanas, e constatou-se a presença de 42 delas, com destaque para conscientização, com onze ocorrências, transformação/ação transformadora e práxis/ação-reflexão, ambas com dez. Com os dados obtidos pelo estudo foi possível constatar o aumento do número de publicações na área da Terapia Ocupacional Social que utilizam o referencial teórico de Freire. A obra do autor tem colaborado de forma significativa na construção de uma Terapia Ocupacional Social problematizadora e emancipatória

Paulo Freire es un autor importante para apoyar la praxis de la Terapia Ocupacional Social. El propósito de este estudio es ampliar el alcance y profundizar en el carácter de la presencia académica de este autor en el campo de la Terapia Ocupacional Social. Como abordaje metodológico, se realizó una revisión de alcance con relevamiento de estudios publicados en cuatro revistas brasileñas y en bases de datos internacionales. Las búsquedas se realizaron solamente en portugués. No se estableció una fecha de inicio y se consideraron artículos disponibles en línea y publicados hasta febrero de 2023. El corpus estuvo constituido por 18 artículos que incluyen obras de Paulo Freire en sus referencias. Pedagogía del oprimido y Educación como práctica de libertadson las obras que más se referencian. Se depuraron las categorías freireanas y se constató la presencia de 42 de ellas, especialmente: concientización, once veces; transformación / acción transformadora y praxis / acción-reflexión, ambas, diez veces. Los resultados permiten constatar el aumento del número de publicaciones en el área de Terapia Ocupacional Social que utilizan el marco teórico de Freire. La obra del autor ha aportado significativamente a la construcción de una Terapia Ocupacional Social problematizadora y emancipadora.

Paulo Freire's work is important to support the praxis of Social Occupational Therapy. The purpose of this study is to expand the scope and deepen the character of the academic presence of Paulo Freire in Social Occupational Therapy. A scoping review was carried out as a methodological approach, including studies in four Brazilian journals and three international databases. The searches were conducted only in Portuguese; no year parameters were stipulated. The review considered articles published at any time up to February 2023. A total of 18 studies with Freire's works in their references were included; "Pedagogy of the Oppressed" and "Education, the Practice of Freedom" are the works that mostly appeared in the articles included. The refinement of the Freirean categories was carried out, and 42 categories were verified: conscientization, eleven times, transformation/transformative action, and praxis/action-reflection, both ten times. With the data obtained in the study, it was possible to verify the increase in the number of publications in Social Occupational Therapy that use Freire's theoretical framework. The author's work has contributed significantly to the construction of a problematizing and emancipating Social Occupational Therapy.

Mosaic trisomy 5 in amniotic fluid in a live fetus with Ebstein anomaly and complete trisomy 5 in chorionic villus sampling.

Complete trisomy 5 is a rare and lethal abnormality. Mosaic trisomy 5 presents in various phenotypes, ranging from a clinically normal fetus to fetuses presenting uterine growth restriction, congenital heart anomalies, multiple dysmorphic features and psychomotor development abnormalities. Although rare, there are cases of a normal psychomotor development regardless of the associated low fetal growth frequently associated with mosaic trisomy 5. This is the first case report to date of a live fetus with complete trisomy 5 reported in chorionic villus sampling and mosaic trisomy 5 in amniotic fluid with a concomitant Ebstein anomaly. Diagnosis of mosaic trisomy 5 represents a challenge for the clinical team and patients, as the information regarding this syndrome is scarce and based mostly on case reports of liveborns, which may introduce a selection bias when counselling the parents.

Efficacy of ultrasound-guided infiltration with levobupivacaine and triamcinolone for myofascial pain syndrome of the quadratus lumborum: a retrospective observational study

Abstract Introduction and objectives: Myofascial Pain Syndrome (MPS) of the Quadratus Lumborum muscle (QL) is a frequent cause of chronic low back pain. With this study, we aimed to assess the efficacy of ultrasound-guided infiltration with 0.25% levobupivacaine and 40 mg triamcinolone for MPS of the QL. Methods: Observational and retrospective study of participants submitted to ultrasound-guided infiltration of the QL muscle from January 1, 2015 to June 31, 2019. Pain intensity was assessed using the five-point pain Numeric Rating Scale (NRS): pre-intervention, at 72 hours, 1 month, 3 months and 6 months post-intervention. Additional data collected were demographic characteristics, opioid consumption, and adverse effects. Results: We assessed 90 participants with mean age of 55.2 years. Sixty-eight percent of participants were female. Compared to the pre-intervention assessment, there was an improvement in pain at 72 hours (Mean Difference [MD = 3.085]; 95% CI: 2.200-3.970, p < 0.05), at the 1st month (MD = 2.644; 95% CI: 1.667-3.621, p < 0.05), at the 3rdmonth (MD = 2.017; 95% CI: 0.202-2.729, p < 0.05) and at the 6th month (MD = 1.339; 95% CI 0.378-2.300, p < 0.05), post-intervention. No statistically significant differences in opioid consumption were observed. No adverse effects associated with the technique were reported. Conclusions: Ultrasound-guided infiltration of the QL muscle is a safe and effective procedure for the treatment of pain in the QL MPS within 6 months post-intervention.

Abordagens em saúde mental para crianças no ambiente escolar: um relato de experiência / Mental health approaches for children in the school environment: an experience report

A escola é um ambiente que pode interferir diretamente na produção social da saúde, integrando bem-estar e educação, assim, o Programa de Saúde na Escola (PSE) visa a integração entre o ambiente escolar e saúde. Contudo, faltam ações que promovam, de forma específica, atividades em saúde mental. Objetivo: relatar a experiência de um grupo de estudantes de Enfermagem da Universidade Federal de Rondônia (UNIR) na abordagem das emoções primitivas com crianças do primeiro ao quarto ano do Ensino Fundamental no ambiente escolar. Resultados: essa experiência demonstrou a importância de ações preventivas e as promoções de saúde mental, por meio do PSE. A abordagem interdisciplinar ressalta a necessidade de cuidar da saúde mental desde a infância, destacando a importância da educação e prevenção nesse contexto. Conclusão: realizar atividades de promoção da saúde mental com crianças em idade escolar exige uma integração multiprofissional e permitiu que houvesse a reflexão sobre a saúde mental infantil e suas complexidades.

The school is an environment that can directly interfere in the social production of health, integrating well-being and education, therefore, the Health at School Program (HSP) aims to integrate the school environment and health. However, there is a lack of actions that specifically promote mental health activities. Objective: to report the experience of a group of nurse school students from Federal University of Rondonia (UNIR) in addressing primitive emotions with children from first to fourth year, in the school environment. Results: This experience demonstrated the importance of preventive and mental health-promoting actions through the Health at School Program (HSP). The interdisciplinary approach highlights the need to take care of mental health from childhood, highlighting the importance of education and prevention in this context. Conclusion: Carrying out activities to promote mental health with school-age children requires multidisciplinary integration and allowed reflection on children's mental health and its complexities.

Instagram e COVID-19: recursos para promoção de saúde de gestantes e puérperas / Instagram and COVID-19: resources for health promotion of pregnant women and mothers / Instagram e COVID-19: recursos para la promoción de la salud de las mujeres embarazadas y las que han dado a luz recientemente

A pandemia da COVID-19 resultou em grande estresse, especialmente para populações mais vulneráveis, como mulheres grávidas e no pós-parto. Devido ao isolamento social, durante o início do período pandêmico, as redes sociais tornaram-se recursos importantes para informar e promover saúde e bem--estar. O objetivo deste estudo é apresentar uma proposta psicoeducativa de intervenção positiva na forma de posts para o Instagram e sua validade de conteúdo para o enfrentamento do estresse provocado pela pandemia por gestantes e puérperas no ano de 2020. A validade de conteúdo foi avaliada por 64 juízas, que analisaram 29 posts adotando uma escala Likert de 5 pontos para validar linguagem, relevância, pertinência e apresentação da proposta. Observou-se coeficientes de validade de conteúdo acima do ponto de corte (≥ 0.80) para todos os critérios, o que indica a adequação da proposta para enfrentar os estressores da pandemia pela população alvo. Dessa forma, confirmou-se a possibilidade de utilização das mídias sociais visando o aumento no acesso a materiais de qualidade que promovem a democratização do cuidado à saúde mental. (AU)

The COVID-19 pandemic has induced significant stress, particularly among vulnerable populations, such as pregnant and postpartum women. Given the social isolation measures implemented at the onset of the pandemic, social media has emerged as a vital tool for disseminating health information and promoting well-being. This study aims to present a psychoeducational inter-vention proposal in the form of Instagram posts and assess its content validity in addressing pandemic-induced stress among pregnant and postpartum women in the year 2020. Content validity was evaluated by 64 female judges who assessed 29 posts using a 5-point Likert scale to validate language, relevance, pertinence, and presentation of the proposal. Results indicated content validity coefficients exceeding the established threshold (≥ 0.80) for all criteria, affirming the suitability of the proposal for mitigating pandemic--related stressors among the target population. Thus, the utilization of social media to enhance access to high-quality resources promoting mental health care democratization is confirmed. (AU)

La pandemia de COVID-19 resultó en un gran estrés, especialmente para pobla-ciones más vulnerables, como mujeres embarazadas y postparto. Debido al aislamiento social durante el periodo pandémico, las redes sociales se volvieron en recursos importantes para informar y promover la salud y el bienestar. El objetivo del estudio es presentar una propuesta psicoeducativa de inter-vención positiva en forma de posts para Instagram y su validez de contenido para el enfrentamiento del estrés provocado por la pandemia por gestantes y puérperas en el año de 2020. La validez del contenido fue evaluada por 64 jueces, que analizaron 29 posts adoptando una escala Likert de 5 puntos para validar lenguaje, relevancia, pertinencia y presentación de la propuesta. Se observaron coeficientes de validez de contenido por encima del punto de corte (≥ 0.80) para todos los criterios, lo que indica la adecuación de la propuesta para enfrentar los estresores de la pandemia por parte de la población obje-tivo. Así, sabemos que es posible utilizar las redes sociales para incrementar el acceso a materiales de calidad que promuevan la democratización de la atención de la salud. (AU)

Frequency of CDH1, CTNNA1 and CTNND1 Germline Variants in Families with Diffuse and Mixed Gastric Cancer.

The most well-characterized hereditary form of gastric cancer is hereditary diffuse gastric cancer (HDGC), an autosomal dominant syndrome characterized by an increased risk of diffuse gastric and lobular breast cancer. HDGC is predominantly caused by germline pathogenic variants in the CDH1 gene, and more rarely in the CTNNA1 gene. Furthermore, the International Gastric Cancer Linkage Consortium (IGCLC) guidelines do not clarify whether or not mixed gastric cancer (with a diffuse component) should be considered in the HDGC genetic testing criteria. We aimed to evaluate the contribution of CTNNA1 and CTNND1 germline variants to HDGC. Additionally, we also intended to compare the frequencies of CDH1 and CTNNA1 (and eventually CTNND1) germline variants between patients with diffuse and mixed gastric carcinomas to evaluate if genetic testing for these genes should or should not be considered in patients with the latter. We analyzed the CDH1 gene in 67 cases affected with early-onset/familial mixed gastric carcinomas and the CTNNA1 and CTNND1 genes in 208 cases with diffuse or mixed gastric cancer who had tested negative for CDH1 pathogenic germline variants. A deleterious CTNNA1 germline variant was found in 0.7% (1/141) of diffuse gastric cancer patients meeting the 2020 IGCLC criteria, as compared to the rate of 2.8% of CDH1 deleterious variants found by us in this setting. No deleterious variants were found in CTNND1, but six variants of uncertain significance were identified in this gene. We did not find any pathogenic CDH1, CTNNA1 or CTNND1 variant in index patients with early-onset/familial mixed gastric cancer, so there is no evidence that supports including this tumor type in the testing criteria for germline variants in these genes. The role of the CTNND1 gene in inherited gastric cancer predisposition is still unclear.

Abordagem da espiritualidade na formação em saúde à luz da teoria de Viktor Frankl / El enfoque de la espiritualidad en la formación en salud a la luz de la teoría de Viktor Frankl / The approach to spirituality in health training in the light of Viktor Frankl's theory

Objetivo: refletir sobre a importância de agregar a espiritualidade na formação dos profissionais de saúde por meio dos aspectos basilares da teoria de Viktor Frankl. Método: trata-se de uma reflexão teórica a partir de Viktor Frankl, discutindo sobre a importância de agregar a espiritualidade na formação dos profissionais de saúde. Resultados: Frankl propõe que o homem é um ser dotado de valores criativos, vivenciais e atitudinais, os quais implicam em processos de devoção dos indivíduos a algo maior que si, especialmente, pela superação das adversidades da vida por meio da conquista de um sentido maior. A espiritualidade é característica central dessa visão, conferindo a autotranscedência, ficando claro a importância de inserir essa dimensão na formação em saúde. Conclusão: A importância de organizar a formação dos profissionais de saúde a partir da inclusão da espiritualidade, segundo os postulados teóricos de Frankl, decorre da necessidade de ir além dos estudos disciplinares e fragmentados das graduações, promovendo uma formação humana e integrativa, que inclua reflexões acerca da própria espiritualidade do profissional, bem como daqueles aos quais este assiste.

Objective: to reflect on the importance of adding spirituality in the training of health professionals through the basic aspects of Viktor Frankl's theory. Methodology: This is a theoretical reflection based on Viktor Frankl, discussing the presence of adding spirituality in the training of health professionals. Results: Frankl defines that man is a being of creative, experiential and attitudinal values, which imply, through the individual, to devote himself to something greater than himself, especially, by overcoming life in the conquest of a meaning. Spirituality is a central feature of this vision, conferring a self-transcendence, making it clear the importance of including this dimension in health education. Conclusion: The importance of organizing the training of health professionals based on the inclusion of spirituality, according to Frankl's theoretical postulates, according to the theoretical postulates of the need to go disciplinary and fragmented of the graduations, promoting a human and integrative formation, which include about of the professional's own spirituality, as well as those who assist.

Objetivo: reflexionar sobre la importancia de sumar la espiritualidad en la formación de los profesionales de la salud a través de los aspectos básicos de la teoría de Viktor Frankl. Metodología: Se trata de una reflexión teórica basada en Viktor Frankl, discutiendo la presencia de la incorporación de la espiritualidad en la formación de profesionales de la salud. Resultados: Frankl define que el hombre es un ser de valores creativos, experienciales y actitudinales, que implican, a través del individuo, entregarse a algo más grande que él mismo, especialmente, por la superación de la vida en la conquista de un sentido. La espiritualidad es un rasgo central de esta visión, confiriendo una autotrascendencia, dejando clara la importancia de incluir esta dimensión en la educación para la salud. Conclusión: La importancia de organizar la formación de los profesionales de la salud a partir de la inclusión de la espiritualidad, según los postulados teóricos de Frankl, según los postulados teóricos de la necesidad de ir disciplinado y fragmentado de las graduaciones, promoviendo una formación humana e integradora, que incluyen de la propia espiritualidad del profesional, así como de los que lo asisten.

Composite pheochromocytoma of the adrenal gland-a review of published cases.

Composite pheochromocytoma (CP) is a rare adrenal tumor, composed of ordinary pheochromocytoma and neuroblastic components. There is a paucity of information in the literature regarding this entity. We report the case of a 56-year-old woman with a CP of the left adrenal gland with a ganglioneuroma component. A review of the published literature found 110 cases of CP. The median age was 51.5 (5.86) years, and 59/110 (53.6%) were female. Association with genetic predisposition syndromes was found in 22/110 (20%), the most common of which was neurofibromatosis type 1, in 15/110 (13.6%). The most common histologic type of the neuroblastic component was ganglioneuroma in 83/110 (75.5%). Twenty-seven cases reported SDHB immunohistochemistry results; none of which was positive. Nine patients (8.2%) presented/developed metastatic disease, and 9 patients (8.2%) died from disease. To our knowledge, this is the largest review describing clinical, histopathological, molecular, and prognostic features of CP.

HPV 18 variants in women with cervical cancer in Northeast Brazil.

BACKGROUND: Human Papillomavirus (HPV) is the main etiological factor for the development of cervical cancer. HPV 18 is the second most frequent type, accounting for up to 65% of all cases. HPV intratypic variation may influence the potential for progression to invasive cancer. The aim of this study was to evaluate the prevalence of human papillomavirus 18 intratypic variants in cervical cancer samples from women in the state of Maranhão, Brazil. METHODS: The study included 118 women over 18 years of age with a diagnosis of cervical cancer. Tumor fragments were collected and subjected to DNA extraction and Polymerase Chain Reaction (PCR) for HPV detection using the PGMY09/11 and GP+5/6 primers. Positive samples were submitted to automated sequencing for viral genotyping. To determine the HPV 18 lineages, positive samples were submitted to PCR, using specific primers to amplify the LCR and E6 regions of HPV 18 virus. RESULTS: HPV was present in 88 women (73.3%). Of those, 48 (54%) were HPV 16, the most prevalent, followed by 12 (13.6%) HPV 18. Histologically, squamous cell carcinoma was predominant (79.1%). Among the HPV 18 variants identified, 10 (80%) belonged to lineage A, and sublineages A1, A2, A3, and A4. Two (29%) HPV 18 B variant was also detected, with the sublineages B1 and B2. In this study, the C variant was not found. There was no statistically significant association between the HPV 18 lineages found and sociodemographic and lifestyle variables (p > 0.05). CONCLUSIONS: A higher frequency of HPV 16 and 18 were found in women with cervical cancer in the state of Maranhão, Brazil, with a high prevalence of the lineage A among women with HPV 18.

Twin Anemia-Polycythemia Sequence (TAPS): From Basic Research to Clinical Practice.

Twin pregnancy is associated with an increased risk of perinatal and maternal complications, and early establishment of the chorionicity type defines this risk. In monochorionic (MC) pregnancies, the fetuses share the same placental mass and exhibit vascular anastomoses crossing the intertwin membrane, and the combination and pattern of anastomoses determine the primary clinical picture and occurrence of future complications. Twin Anemia-Polycythemia Sequence (TAPS) was first described in 2006 after fetoscopic laser surgery in twin-to-twin transfusion syndrome (TTTS) twins, and in 2007, the first spontaneous cases were reported, recognizing TAPS as an individualized vascular identity in fetofetal transfusion syndromes. There are two types of TAPS: spontaneous (3-5%) and iatrogenic or postlaser (2-16%). TAPS consists of small diameter arteriovenous anastomoses (<1 mm) and low-rate, small-caliber AA anastomoses in the absence of amniotic fluid discordances. There are certain antenatal and postnatal diagnostic criteria, which have progressively evolved over time. New, additional secondary markers have been proposed, and their reliability is being studied. The best screening protocol for TAPS in MC twins is still a matter of debate. This review provides a survey of the relevant literature on the epidemiology, vascular pathophysiology, underlying hemodynamic factors that regulate mismatched vascular connections, and diagnostic criteria of this condition. The aim is to increase awareness and knowledge about this recently identified and frequently unrecognized and misdiagnosed pathology.

Nickel Allergy After a Negative Test Result Following Nuss Procedure for Pectus Excavatum: a Case Report.

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Percepção de risco e comportamentos de proteção face à covid-19 em estudantes do ensino superior: o papel mediador do medo / Percepción del riesgo y conductas protectoras frente al covid-19 en estudiantes de enseñanza superior: el papel mediador del miedo / Risk Perception and Protective Behaviors Against covid-19 in Higher Education Students: The Mediating Role of Fea

O estudo tem como objetivo descrever os comportamentos de proteção adotados por estudantes portugue-ses do ensino superior durante a pandemia e analisar a sua relação com a percepção de risco e o papel mediador do medo face à covid-19. Participaram 335 estu-dantes com idades entre os 18 e os 29 anos (m= 21.42; dp= 2.43). Os participantes preencheram um inquérito sobre os comportamentos de proteção (i.e., preventi-vos e de evitamento), a percepção de risco e o medo face à covid-19. Os resultados demonstraram que os comportamentos preventivos mais utilizados foram a lavagem/desinfecção das mãos ao longo do dia e o uso de máscara na via pública ou espaços exteriores. Os comportamentos de evitamento mais adotados foram o de evitar locais com aglomeração e os convívios presenciais com colegas/amigos. Verificouse uma as-sociação positiva entre os comportamentos de proteção, a percepção de gravidade e o medo face à covid-19. O medo face à covid-19 foi mediador da relação entre a percepção de gravidade e os comportamentos preventivos (c' = .26, ic 95% [.11; .44]) e de evitamento (c' = .28, ic 95% [.12; .50]). Os resultados são discutidos aten-dendo ao papel da percepção de risco e do medo nas respostas comportamentais dos jovens, num contexto de pandemia, sendo apresentadas implicações práticas e sugestões para estudos futuros.

El estudio tiene como objetivo describir las conductas protectoras adoptadas por los estudiantes portugueses de educación superior durante la pandemia, y analizar su relación con la percepción de riesgo y el papel mediador del miedo al covid-19. Participaron en el estudio 335 estudiantes de entre 18 y 29 años (m = 21.42; ds = 2.43). Los participantes completaron una encuesta sobre conductas protectoras (es decir, preventivas y de evitación), percepción de riesgo y miedo al covid-19. Los resultados mostraron que las conductas preventivas más utilizadas fueron el lavado/desinfección de manos a lo largo del día y el uso de mascarilla en la vía pública o al aire libre. Los comportamientos de evitación más adoptados fueron evadir lugares con reuniones e interacciones cara a cara con compañeros/amigos. Hubo una correlación positiva entre las conductas protectoras, la percepción de seriedad y el miedo al covid-19. También se encontró que el miedo al covid-19 medió la relación entre la percepción de severidad y las conductas preventivas (c' = .26, ic 95% [.11; .44]) y de evitación (c' = .28, ic 95% [.12; .50]). Los resultados se discuten considerando el papel de la percepción del riesgo y el miedo en las respuestas conductuales de los jóvenes en un contexto pandémico, así mismo se presentan impli-caciones prácticas y sugerencias para estudios futuros.

The study aims to describe the protective behaviors adopted by Portuguese higher education students in during the and to analyze their relationship with the perception of risk and the mediating role of fear of covid-19. A total of 335 students aged be-tween 18 and 29 participated in the study (m= 21.42; sd = 2.43). Participants completed a survey on protective (i.e., preventive and avoidance) behaviors, risk perception, and fear of covid-19. The results showed that the most used preventive behaviors were hand washing/disinfection throughout the day and using masks in public streets or outdoor spaces. The most adopted avoidance behaviors were avoiding crowded places and face-to-face interactions with colleagues/friends. There was a positive correlation between pro-tective behaviors, the perception of severity, and fear of covid-19. Fear of covid-19 mediated the relation between the perception of severity and both preventive (c' = .26, ic 95% [.11; .44]) and avoidance (c' = .28, ic95% [.12; .50]) behaviors. The results are discussed considering the role of risk perception and fear in the behavioral responses of young people in a pandemic context while presenting practical implications and suggestions for future studies.

Dream-enactment behaviours during the COVID-19 pandemic: an international COVID-19 sleep study.

There has been increasing concern about the long-term impact of coronavirus disease 2019 (COVID-19) as evidenced by anecdotal case reports of acute-onset parkinsonism and the polysomnographic feature of increased rapid eye movement sleep electromyographic activity. This study aimed to determine the prevalence and correlates of dream-enactment behaviours, a hallmark of rapid eye movement sleep behaviour disorder, which is a prodrome of α-synucleinopathy. This online survey was conducted between May and August 2020 in 15 countries/regions targeting adult participants (aged ≥18 years) from the general population with a harmonised structured questionnaire on sleep patterns and disorders, COVID-19 diagnosis and symptoms. We assessed dream-enactment behaviours using the Rapid Eye Movement Sleep Behaviour Disorder Single-Question Screen with an additional question on their frequency. Among 26,539 respondents, 21,870 (82.2%) answered all items that were analysed in this study (mean [SD] age 41.6 [15.8] years; female sex 65.5%). The weighted prevalence of lifetime and weekly dream-enactment behaviours was 19.4% and 3.1% and were found to be 1.8- and 2.9-times higher in COVID-19-positive cases, respectively. Both lifetime and weekly dream-enactment behaviours were associated with young age, male sex, smoking, alcohol consumption, higher physical activity level, nightmares, COVID-19 diagnosis, olfactory impairment, obstructive sleep apnea symptoms, mood, and post-traumatic stress disorder features. Among COVID-19-positive cases, weekly dream-enactment behaviours were positively associated with the severity of COVID-19. Dream-enactment behaviours are common among the general population during the COVID-19 pandemic and further increase among patients with COVID-19. Further studies are needed to investigate the potential neurodegenerative effect of COVID-19.

Preoperative Metal Patch Testing and Titanium Bar Use Criteria in Nuss Procedure: A 56-Patients' Cohort Study.

INTRODUCTION: Nuss procedure is the main reason for metal implants use by pediatric and thoracic surgeons. There is an ongoing debate on how to avoid allergic complications. Herein we describe our 8-year experience with systematic preoperative metal patch testing and our selective titanium bar use in Nuss procedure. MATERIALS AND METHODS: This is a single center retrospective observational cohort study of patients who underwent the Nuss procedure from 2013 to 2020. Preoperative metal patch testing was done in all cases. Criteria for titanium bar utilization were: a positive test for a major component of the stainless-steel bar; or a positive metal patch test and a positive history of atopy, food or metal allergy, or previous allergic reaction to an implant or device. RESULTS: In total, 56 patients were included. Most were male (91.1%) with a median age of 15.0 (13.0-22.0) years old. 19.6% had a positive preoperative metal patch test and 54.5% of these had no personal history of atopy. Stainless-steel bars were used in 27.3% of those patients and titanium bars were used in 72.7%. One patient had a documented minor allergy reaction. None of the 56 patients required early bar removal. CONCLUSION: Our study suggests that routine preoperative allergy testing and a judicious use of titanium bar are safe and avoid metal allergic complications.