RESUMO
The cytogenetic damage in floriculturists of Morelos State, Mexico, exposed to pesticides, was evaluated by mean of biological tests based on sister chromatid exchanges (SCE) in lymphocytes of peripheral blood and micronuclei (MN) in exfoliated cells of the buccal mucosa. Besides the cytogenetic analysis, the effects of pesticides exposure on the cell proliferation kinetics (CPK) by the replication index (RI) were also studied. The mitotic index (MI) to detect cytotoxic effects was also determined. Greenhouses of the towns of Santa Catarina, Jiutepec and Yecapixtla were selected for the study, because the application of chemicals to the flowers is uncontrolled. As non-exposed group, people of the town of Temisco were chosen; their activity was not related to pesticides. The SCE were analyzed in the peripheral blood of 30 persons, 22 women and 8 men, with 10 and 1.5 years of exposure to pesticides, respectively, and of 30 persons, 28 women and 2 men, that were considered as the non-exposed group. Samples of buccal mucosa were also taken from each person. Significant differences between exposed and non-exposed groups were found in SCE, CKP and MI. Besides, the MN frequencies in the exposed group were three times higher than in the non-exposed group.