Potencia y actividad electromiográfica en voleibolistas universitarios / Power and electromyographic activity in college volleyball players

Resumen El propósito del estudio fue determinar la potencia relativa del miembro inferior y actividad electromiográfica de superficie (EMGs) del glúteo mayor (GM), vasto medial (VM), vasto lateral (VL) y gastrocnemio lateral (GASLAT), durante un salto contramovimiento (CMJ) y un salto Abalakov (ABK). Un total de 24 voleibolistas universitarios se dividieron en dos grupos iguales asignados por el nivel de competencia: el grupo 1 (G1), compuesto por voleibolistas de 1ª división universitaria y el grupo 2 (G2), compuesto por voleibolistas de 2ª división universitaria. Cada uno fue sometido a dos evaluaciones simultáneas de potencia y EMGs. Para la potencia se utilizó un sistema de grabación en 2D, por medio de un seguimiento del trocánter mayor con una cámara de 250 fps. Esta grabación se sometió a una medición por medio de software (Tracker), para obtener los valores de potencia absoluta. La EMGs se realizó por medio de un electromiógrafo Delsys Trigno en los músculos GM, VM, VL y GASLAT. Se presentaron diferencias significativas en %PeakRMS del VL (G1=65.210.2; G2=54.0±11.7 %PeakRMS; p0.05) y ABK (p>0.05). G.=50.6±7.2 %PeakRMS; p0.05) y ABK (p>0.05).

Abstract The purpose of this study was to determine the relative power of lower limbs and the surface electromyographic activity (EMG) of the gluteus maximus (GM), vastus medialis (VM), vastus lateralis (VL), and lateral gastrocnemius (GASLAT) during a countermovement jump (CMJ) and an Abalakov jump (ABK). Twenty-four college volleyball players were divided into two equal groups assigned by division: Group 1 (G1), comprised of volleyball players from the 1st university division, and Group 2 (G2), comprised of volleyball players from the 2nd university division. Both groups took two simultaneous EMG and power assessments. For the power assessment, a 2D recording system was used to track the greater trochanter with a 250fps camera. The video was analyzed with the Tracker 4.96 software to obtain absolute power values. The EMG activity was measured using a Delsys Trigno® electromyograph in the GM, VM, VL, and GASLAT muscles. Significant differences were found in %PeakRMS of VL (G1=65.210.2; G2=54.0±11.7 %PeakRMS; p 0.05) and ABK (p>0.05).

Resumo Este estudo teve como objetivo determinar a potência relativa do membro inferior e atividade eletromiográfica (EMG) de superfície do glúteo máximo (GM), vasto medial (VM), vasto lateral (VL) e gastrocnêmio lateral (GASLAT) durante um salto contramovimento (CMJ) e um salto Abalakov (ABK). No total foram 24 jogadores de vôlei universitários divididos em dois grupos iguais, determinados pelo nível de competição: o grupo 1 (G1), composto por jogadores de 1ª divisão universitária e o grupo 2 (G2), composto por jogadores de vôlei de 2ª divisão universitária. Cada um foi submetido a duas avaliações simultâneas de potência e EMG de superfície. Para a potência foi utilizada um sistema de gravação 2D, por meio de um segmento do trocânter maior com uma câmara de 250 Fps. Essa gravação foi submetida a uma medição por meio de software (Tracker), para obter os valores de potência absoluta. A EMG de superfície foi realizada por meio do aparelho de eletromiografia Delsys Trigno nos músculos GM, VM, VL e GASLAT. Houve diferenças significativas em %PeakRMS do VL (G1=65,210,2; G2=54,0±11,7 %PeakRMS; p0,05) e ABK (p>0,05).

Selected Probiotic Lactobacilli Have the Capacity To Hydrolyze Gluten Peptides during Simulated Gastrointestinal Digestion.

The aim of this study was to demonstrate the capacity of probiotic lactobacilli to hydrolyze immunogenic gluten peptides. Eighteen commercial strains of probiotic lactobacilli with highly variable peptidase activity (i.e., aminopeptidase N, iminopeptidase, prolyl endopeptidyl peptidase, tripeptidase, prolidase, prolinase, and dipeptidase), including toward Pro-rich peptides, were tested in this study. Ten probiotic strains were selected on the basis of their specific enzyme activity. When pooled, these 10 strains provided the peptidase portfolio that is required to completely degrade the immunogenic gluten peptides involved in celiac disease (CD). The selected probiotic mixture was able to completely hydrolyze well-known immunogenic epitopes, including the gliadin 33-mer peptide, the peptide spanning residues 57 to 68 of the α9-gliadin (α9-gliadin peptide 57-68), A-gliadin peptide 62-75, and γ-gliadin peptide 62-75. During digestion under simulated gastrointestinal conditions, the pool of 10 selected probiotic lactobacilli strongly hydrolyzed the wheat bread gluten (ca. 18,000 ppm) to less than 10 ppm after 360 min of treatment. As determined by multidimensional chromatography (MDLC) coupled to nanoelectrospray ionization (nano-ESI)-tandem mass spectrometry (MS/MS), no known immunogenic peptides were detected in wheat bread that was digested in the presence of the probiotics. Accordingly, the level of cytokines (interleukin 2 [IL-2], IL-10, and interferon gamma [IFN-γ]) produced by duodenal biopsy specimens from CD patients who consumed wheat bread digested by probiotics was similar to the baseline value (negative control). Probiotics that specifically hydrolyze gluten polypeptides could also be used to hydrolyze immunogenic peptides that contaminate gluten-free products. This could provide a new and safe adjunctive therapy alternative to the gluten-free diet (GFD).IMPORTANCE This study confirmed that probiotic Lactobacillus strains have different enzymatic abilities for hydrolyzing polypeptides, including the Pro-rich epitopes involved in the pathology of CD. Ten lactobacilli with complementary peptidase activities that hydrolyze gluten peptides during simulated gastrointestinal digestion were selected and tested. The results collected showed the potential of probiotic formulas as novel dietary treatments for CD patients.

Exploration of the genomic diversity and core genome of the Bifidobacterium adolescentis phylogenetic group by means of a polyphasic approach.

In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S internal transcribed spacer (ITS) sequences, pulsed-field gel electrophoresis (PFGE), and comparative genomic hybridization (CGH) assays.

Quantification of phenyllactic acid in wheat sourdough using high resolution gas chromatography-mass spectrometry.

In this study, high-resolution gas chromatography-mass spectrometry (HRGC-MS) was successfully used to quantify the level of phenyllactic acid produced by Lactobacillus plantarum strains during sourdough fermentation. Investigation of samples collected during fermentation revealed that the production of phenyllactic acid occurs throughout the growth of L. plantarum in sourdough, but the highest production rate was observed during the logarithmic growth phase. The highest amount, that is, 33.47 mg of phenyllactic acid/kg of dough, was measured in sourdough fermented by the antifungal strain L. plantarum FST 1.7. Sourdoughs fermented by different L. plantarum strains contained different amounts of phenyllactic acid, thus indicating that the production is strain-dependent. Phenylacetic acid was also detected during sourdough analysis, thus showing that the HRGC-MS protocol developed is suitable for the detection not only of phenyllactic acid, but also of a broader range of phenolic acids that are highly relevant, but present in very low amounts in sourdough.