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J Cyst Fibros ; 13(6): 623-31, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24713593

RESUMO

BACKGROUND: How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection. METHODS: We determined the temperature in the exhaled air of cystic fibrosis (CF) patients. To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq. RESULTS: Here we show a temperature of ~38°C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise. Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38°C vs 30°C revealed increased virulence traits and characteristic cell wall changes. CONCLUSION: Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF.


Assuntos
Temperatura Corporal , Fibrose Cística/enzimologia , Elastase de Leucócito/fisiologia , Infecções Respiratórias/enzimologia , Adolescente , Animais , Estudos de Casos e Controles , Criança , Fibrose Cística/complicações , Modelos Animais de Doenças , Feminino , Temperatura Alta , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Infecções por Pseudomonas/enzimologia , Infecções por Pseudomonas/microbiologia , Infecções por Pseudomonas/patologia , Pseudomonas aeruginosa , Infecções Respiratórias/microbiologia , Infecções Respiratórias/patologia
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