Prevalence of hemotropic mycoplasmas and coinfection with feline leukemia virus and feline immunodeficiency virus in cats in the Moscow region, Russia.

Feline haemoplasma infection studies are lacking in Russia. This retrospective study was conducted to estimate the prevalence of feline haemoplasmas in domestic cats in the Moscow region, Russia. A risk of haemoplasma coinfection with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) was also determined. qPCR analysis for feline haemoplasmas was performed on EDTA blood samples from 753 cats from the Moscow region, Russia. Subsets of these samples were tested also for FIV and FeLV by qPCR. Of the 753 blood samples, 104 (13.8 %) were positive for one of the Mycoplasma species. The prevalence of 'Candidatus Mycoplasma haemominutum' (CMhm), Mycoplasma haemofelis (Mhf), and 'Candidatus Mycoplasma turicensis' (CMt) was 7.6 %, 5.5 %, and 0.7 %, respectively. One sample (0.1 %) was simultaneously infected with two haemoplasmas, namely, Mhf and CMt. Haemoplasma positive cats were more likely to be infected with FIV than haemoplasma negative (17.6 % vs 6.7 %), but these differences were not statistically significant. The prevalence of FeLV was comparable among haemoplasma positive and negative cats (23.5 % vs 25.7 %) All three known species of feline haemoplasma were detected, confirming their presence in Russia. The overall and species-specific rates of haemoplasma infections in Russian cats are generally similar to the rates in the countries of central Europe. This report documents for the first time the prevalence of feline hemotropic mycoplasmas in domestic cats not only in Russia but also in eastern Europe.

Alterations in gene expression of proprotein convertases in human lung cancer have a limited number of scenarios.

Proprotein convertases (PCs) is a protein family which includes nine highly specific subtilisin-like serine endopeptidases in mammals. The system of PCs is involved in carcinogenesis and levels of PC mRNAs alter in cancer, which suggests expression status of PCs as a possible marker for cancer typing and prognosis. The goal of this work was to assess the information value of expression profiling of PC genes. Quantitative polymerase chain reaction was used for the first time to analyze mRNA levels of all PC genes as well as matrix metalloproteinase genes MMP2 and MMP14, which are substrates of PCs, in 30 matched pairs of samples of human lung cancer tumor and adjacent tissues without pathology. Significant changes in the expression of PCs have been revealed in tumor tissues: increased FURIN mRNA level (p<0.00005) and decreased mRNA levels of PCSK2 (p<0.007), PCSK5 (p<0.0002), PCSK7 (p<0.002), PCSK9 (p<0.00008), and MBTPS1 (p<0.00004) as well as a tendency to increase in the level of PCSK1 mRNA. Four distinct groups of samples have been identified by cluster analysis of the expression patterns of PC genes in tumor vs. normal tissue. Three of these groups covering 80% of samples feature a strong elevation in the expression of a single gene in cancer: FURIN, PCSK1, or PCSK6. Thus, the changes in the expression of PC genes have a limited number of scenarios, which may reflect different pathways of tumor development and cryptic features of tumors. This finding allows to consider the mRNAs of PC genes as potentially important tumor markers.

Cathepsin D messenger RNA is downregulated in human lung cancer.

OBJECTIVES: Lysosomal proteases cathepsins B and D (CB and CD) play a significant part in cancer progression. For many oncological diseases protein expression levels of CB and CD have been investigated and correlations with tumour characteristics revealed. Meanwhile, there is very little information concerning mRNA expression level. METHODS: In the present work, data about mRNA levels of CB and CD in human lung cancer was obtained using reverse transcription followed by real-time polymerase chain reaction. RESULTS: For the first time CD and CB mRNA in human lung cancer tumours was quantified. It was shown that CB and CD mRNA levels do not correlate with any tumour characteristics. However, in most analysed tumours, expression of CD mRNA was downregulated compared with adjacent normal tissue (p <0.0003). CONCLUSIONS: The data obtained indicate CD mRNA as a potential lung cancer marker.

Detection and species identification of four human herpesviruses using polymerase chain reaction coupled with restriction endonuclease analysis.

A polymerase chain reaction (PCR) based assay for detection and species identification of four human herpesviruses, including herpes simplex virus types 1 and 2, Epstein-Barr virus, and cytomegalovirus was developed. The detection of the herpesviruses was achieved by seminested PCR with three primers targeting well-conserved regions within the DNA-polymerase gene. Virus species were identified by simple restriction enzyme digestion of the amplified products with TaqI or RsaI. In comparison with mono-specific nested PCR assays the tetra-specific assay demonstrated similar specificity and sensitivity with reference and clinical samples. The tetra-specific assay is sensitive, cost effective, and can be used for examination of clinical samples of different origin.