Platelet-rich plasma (PRP) and tendon healing: comparison between fresh and frozen-thawed PRP.

Platelet-rich plasma (PRP) is increasingly used in the treatment of musculoskeletal diseases. Its preservation by freezing it for the realization of multiple injections in clinical use has never been discussed. Calcaneal tendons of rats were surgically sectioned. Platelet concentration of the PRP was 2.5 x 106/µl with autologous plasma of rats. Frozen-thawed PRP was prepared by performing two cycles of freezing and thawing on PRP aliquots. Both platelet preparations were injected in the lesion. Biomechanical and histological evaluations were carried out after 7, 20 or 40 days post surgery. After 7 and 40 days, no significant difference was observed between the PRP and the frozen-thawed PRP group. There is however a difference 20 days after surgery: the ultimate tensile strength (UTS) was greater in the fresh PRP group. No obvious difference with histological aspect was observed between the two groups. In conclusion, fresh PRP and frozen-thawed PRP injections can lead to similar results in the healing process of section calcaneal tendons of rats. Improvements with fresh PRP are slight. PRP could thus be frozen to be preserved if multiple injections are needed (e.g. osteoarthritis).

Dominance effect on scapula 3-dimensional posture and kinematics in healthy male and female populations.

BACKGROUND: The contralateral shoulder is often used as a reference when evaluating a pathologic shoulder. However, the literature provides contradictory results regarding the symmetry of the scapular pattern in a healthy population. We assume that several factors including gender and type of motion may influence the bilateral symmetry of the scapulae. MATERIALS AND METHODS: The dominant and nondominant shoulders of 2 populations of men and women comprising 11 subjects each were evaluated for 3 distinct motions: flexion in the sagittal plane, abduction in the frontal plane, and glenohumeral internal/external rotation with the arm abducted at 90°. Posture, kinematics, and range of motion were studied separately. RESULTS: Asymmetries are observed for motions performed in the frontal and sagittal plane but not for internal/external rotation with the arm abducted at 90°. For both male and female populations, multiplanar asymmetries are observed and the dominant scapula has a larger upward rotation. The asymmetries mainly originate in the scapula's kinematics and not in its original posture. CONCLUSION: Small but significant asymmetries exist between the dominant and nondominant shoulders in terms of kinematics. One should be aware of these differences when using the contralateral shoulder as a reference. LEVEL OF EVIDENCE: Basic science study, kinematics