Assuntos
Automação Laboratorial , Vírus BK/genética , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/normas , Infecções por Polyomavirus/diagnóstico , Infecções Tumorais por Vírus/diagnóstico , Automação Laboratorial/métodos , Automação Laboratorial/normas , Transplante de Medula Óssea/efeitos adversos , Transplante de Medula Óssea/normas , DNA Viral/análise , DNA Viral/genética , Humanos , Transplante de Rim/efeitos adversos , Transplante de Rim/normas , Tipagem Molecular/métodos , Tipagem Molecular/normas , Segurança do Paciente/normas , Reação em Cadeia da Polimerase/instrumentação , Infecções por Polyomavirus/transmissão , Infecções por Polyomavirus/virologia , Guias de Prática Clínica como Assunto/normas , Padrões de Referência , Transplantados , Infecções Tumorais por Vírus/transmissão , Infecções Tumorais por Vírus/virologia , Carga Viral , Organização Mundial da SaúdeRESUMO
We here report the case of a 52-year old man who presented hepatitis with hemophagocytic syndrome triggered by herpes simplex 1 (HSV-1) primary infection. A high-level viremia was observed, and HSV-1 DNA remained detectable in blood for a long time after patient's recovery.
Assuntos
DNA Viral/sangue , Hepatite Viral Humana/virologia , Herpes Simples/virologia , Herpesvirus Humano 1/genética , Linfo-Histiocitose Hemofagocítica/etiologia , Aciclovir/uso terapêutico , Etoposídeo/uso terapêutico , Hepatite Viral Humana/sangue , Hepatite Viral Humana/tratamento farmacológico , Herpes Simples/sangue , Herpes Simples/complicações , Herpes Simples/tratamento farmacológico , Herpesvirus Humano 1/efeitos dos fármacos , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Linfo-Histiocitose Hemofagocítica/sangue , Linfo-Histiocitose Hemofagocítica/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Carga Viral/efeitos dos fármacosRESUMO
In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC) set up the third annual series of workshops which brought together practitioners from all member centers and took place in October 2012 in Lille. Here we report our results and recommendations regarding the management of virus respiratory syncytial virus (RSV), human herpes virus 6 (HHV6) or adenovirus allogeneic Stem Cell Transplantation.
Assuntos
Infecções por Adenoviridae/terapia , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Infecções por Vírus Respiratório Sincicial/terapia , Infecções por Roseolovirus/terapia , Ativação Viral/fisiologia , Adenoviridae/fisiologia , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/etiologia , Consenso , Seleção do Doador/normas , Transplante de Células-Tronco Hematopoéticas/normas , Transplante de Células-Tronco Hematopoéticas/estatística & dados numéricos , Herpesvirus Humano 6/fisiologia , Humanos , Terapia de Imunossupressão/normas , Terapia de Imunossupressão/estatística & dados numéricos , Infecções por Vírus Respiratório Sincicial/epidemiologia , Infecções por Vírus Respiratório Sincicial/etiologia , Vírus Sinciciais Respiratórios/fisiologia , Infecções por Roseolovirus/epidemiologia , Infecções por Roseolovirus/etiologia , Transplante HomólogoRESUMO
In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC) set up the third annual series of workshops which brought together practitioners from all member centers and took place in October 2012 in Lille. Here we report our results and recommendations regarding the management of pre-transplant donor's cytomegalovirus, Epstein-Barr virus, Toxoplasma gondii, or syphilis IgM positive serology test.
Assuntos
Infecções por Citomegalovirus/diagnóstico , Seleção do Doador/normas , Infecções por Vírus Epstein-Barr/diagnóstico , Transplante de Células-Tronco Hematopoéticas/normas , Achados Incidentais , Sífilis/diagnóstico , Toxoplasmose/diagnóstico , Doadores de Sangue , Consenso , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/sangue , Infecções por Vírus Epstein-Barr/sangue , França , Conhecimentos, Atitudes e Prática em Saúde , Herpesvirus Humano 4/isolamento & purificação , Humanos , Imunoglobulina M/sangue , Sífilis/sangue , Sífilis/imunologia , Toxoplasma/isolamento & purificação , Toxoplasmose/sangue , Transplante HomólogoRESUMO
In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapy (SFGM-TC) set up the third annual series of workshops which brought together practitioners from all member centers and took place in October 2012 in Lille. Here we report our results and recommendations regarding the management of common issues related to the donor: pre-transplant pregnancy and monoclonal gammopathy.
Assuntos
Doadores de Sangue , Seleção do Doador/normas , Conhecimentos, Atitudes e Prática em Saúde , Transplante de Células-Tronco Hematopoéticas/normas , Achados Incidentais , Paraproteinemias/diagnóstico , Testes de Gravidez , Consenso , Feminino , Idade Gestacional , Humanos , Paraproteinemias/sangue , Gravidez/sangue , Complicações na Gravidez/sangue , Complicações na Gravidez/prevenção & controleRESUMO
BACKGROUND: High-risk HPV (HR-HPV) are associated with the development of cervical cancer, the most common cancer in women in developing countries. Reliable diagnosis of HR-HPV infection combined with simple procedures to collect and store biological samples, could improve primary screening programs and vaccine strategies in these areas. OBJECTIVE: To evaluate HR-HPV detection in conventional and dried samples. STUDY DESIGN: The presence of HR-HPV in 31 women in Republic of Congo (Central Africa) has been investigated by using standard cervical samples and dried cervical samples collected on filter paper and vaginal tampons. The detection of HPV DNA was performed in the Laboratory of virology in Lille (France) by using Hybrid capture 2 and HPV 16/18/45 Probe Set Test. RESULTS: 22 standard samples were found positive for the detection of HR-HPV (71%). HPV 16/18/45 was displayed in 15 out of 22 samples positive for HR-HPV (68%). The correlations between HPV detection by using standard samples and samples dried on filter paper and dried tampons were 90.3% (kappa = 0.77) and 80.6% (kappa = 0.5) respectively. The sensitivity and the specificity of HPV detection reached 91% and 89%, respectively, with samples dried on filter paper and were 86% and 67%, respectively, for dried tampons compared with standard samples. CONCLUSION: Dried cervical samples and dried vaginal tampons can represent an alternative to conventional sampling to reduce barriers to large screening programs in developing countries and to facilitate storage and transport to reference centers.
Assuntos
Colo do Útero/virologia , Filtração/métodos , Produtos de Higiene Menstrual/virologia , Papillomaviridae/genética , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/diagnóstico , Manejo de Espécimes/métodos , Neoplasias do Colo do Útero/diagnóstico , Congo , DNA Viral/análise , DNA Viral/genética , Dessecação , Feminino , Papillomavirus Humano 16/genética , Papillomavirus Humano 16/isolamento & purificação , Papillomavirus Humano 18/genética , Papillomavirus Humano 18/isolamento & purificação , Humanos , Papillomaviridae/classificação , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/virologia , Risco , Sensibilidade e Especificidade , Neoplasias do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/virologiaRESUMO
Human herpesvirus-6 (HHV-6) has been identified as the causal agent of exanthema subitum in early childhood (also called sixth disease or roseola), a mononucleosis-like disease in adults, and as an opportunistic pathogen in transplant recipients. In the latter setting, most infections are caused by reactivation of the latent virus in the recipient and generally have a paucisymptomatic course. Only limited data on HHV-6 infection are available for liver transplant recipients. Herein we have reported a case of fatal hemophagocytic syndrome related to HHV-6 reactivation 2 weeks after liver transplantation (LT). This case suggests that this virus may be a serious and potentially life-threatening pathogen following LT.
Assuntos
Herpesvirus Humano 6 , Transplante de Rim/efeitos adversos , Transplante de Fígado/efeitos adversos , Linfo-Histiocitose Hemofagocítica/virologia , Doenças Renais Policísticas/cirurgia , Infecções por Roseolovirus/complicações , Infecções por Citomegalovirus/complicações , Evolução Fatal , Feminino , Humanos , Pessoa de Meia-Idade , RecidivaRESUMO
Hepatitis C virus (HCV) is an enveloped virus and encodes two envelope glycoproteins, E1 and E2. E1 and E2 are transmembrane type I proteins with a N-terminal ectodomain and C-terminal anchor. During their synthesis, E1 and E2 ectodomains are targeted in the endoplasmic reticulum lumen where they are modified by N-linked glycosylation. After their synthesis, E1 and E2 assemble as a non-covalent heterodimer. The N-linked glycosylation is based on the recognition of specific asparagine residue in the context of the consensus sequence Asn-X-Ser/Thr. E1 contains potentially 4 or 5 N-linked glycosylation sites and E2 up to 11. Recent data indicated that some glycans of glycoproteins E1 and E2 play a major role in protein folding and heterodimer formation. Some N-linked glycans of E2 were involved in interactions with CD81, a putative cellular receptor for HCV. It appeared that N-linked glycans of E1 and E2 played an important role of in the viral entry.
Assuntos
Hepacivirus/metabolismo , Polissacarídeos/fisiologia , Proteínas do Envelope Viral/fisiologia , Glicosilação , Hepacivirus/patogenicidadeRESUMO
We report a case of a 15-year-old young man who was admitted for an acute hepatitis. Virological assessment showed both IgM anti-EBV and IgM anti-hepatitis A. IgG anti-EBNA and clinical history allowed to rule out the hypothesis of a recent EBV infection and confirmed the diagnosis of acute hepatitis A infection.
Assuntos
Vírus da Hepatite A/imunologia , Hepatite A/diagnóstico , Herpesvirus Humano 4/imunologia , Imunoglobulina M/análise , Doença Aguda , Adolescente , Emergências , Seguimentos , Hepatite A/imunologia , Humanos , Unidades de Terapia Intensiva , Masculino , Fatores de TempoRESUMO
The potential role of an immune response in HPV-related anogenital disorders had already been anticipated by clinicians. Indeed the lesions efflorescence and the relapsing HPV infection in HIV positive patients as well as the lack of recurrence in patients with spontaneous cure, provided relevant clues for a likely immune mechanism. At present time, the role of the immune system in the development of HPV-related anogenital disorders is well established : HPV induce a humoral and cell mediated immune response. This response is mainly exerted towards infected cells; it is also exerted at the systemic level, through antibodies synthesis, but this pathway remains a secondary one. Due to the limits of the present therapies (either purely destructive and characterized by the rate of recurrences, or antiviral, but difficult to use), it was necessary to find a new treatment type which enhances the local immune response, results in the disappearance of lesions and allows for a decrease in the risk of recurrences. The original mechanism of action of the first cell-mediated immune response modifier: imiquimod, for local use (Aldara 5 % cream) is an answer to this need. The first positive results observed in vitro and in animals were confirmed in patients with HPV anogenital warts in a double blind placebo-controlled study: imiquimod inhibits HPV replication and results in the condyloma regression. Its action is based on the combined activation of the natural local immunity, by stimulating interferon alpha; and of the acquired immunity, by stimulating a T-cell mediated immune response. Thus imiquimod appears to be an original antiviral compound, because it does not act directly on the virus itself.
Assuntos
Adjuvantes Imunológicos/farmacologia , Adjuvantes Imunológicos/uso terapêutico , Aminoquinolinas/farmacologia , Aminoquinolinas/uso terapêutico , Antivirais/farmacologia , Antivirais/uso terapêutico , Doenças do Ânus/tratamento farmacológico , Condiloma Acuminado/tratamento farmacológico , Doenças dos Genitais Femininos/tratamento farmacológico , Doenças dos Genitais Masculinos/tratamento farmacológico , Indutores de Interferon/farmacologia , Indutores de Interferon/uso terapêutico , Papillomaviridae/efeitos dos fármacos , Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Infecções Oportunistas Relacionadas com a AIDS/imunologia , Adjuvantes Imunológicos/administração & dosagem , Adulto , Aminoquinolinas/administração & dosagem , Animais , Anticorpos Antivirais/análise , Antivirais/administração & dosagem , Doenças do Ânus/imunologia , Doenças do Ânus/cirurgia , Condiloma Acuminado/imunologia , Condiloma Acuminado/cirurgia , Modelos Animais de Doenças , Feminino , Seguimentos , Doenças dos Genitais Femininos/imunologia , Doenças dos Genitais Femininos/cirurgia , Doenças dos Genitais Masculinos/imunologia , Doenças dos Genitais Masculinos/cirurgia , Soropositividade para HIV , Haplorrinos , Humanos , Imiquimode , Imunidade Celular , Indutores de Interferon/administração & dosagem , Masculino , Camundongos , Pomadas , Papillomaviridae/imunologia , Placebos , Ensaios Clínicos Controlados Aleatórios como Assunto , Ratos , Recidiva , Linfócitos T Citotóxicos/imunologia , Fatores de Tempo , Resultado do TratamentoRESUMO
Cytomegalovirus (CMV) screening during pregnancy has been widely discussed for several years, but still no consensus has been agreed. With a number of live births of 750,000 per year in France, we would expect 7500 infected infants at birth per year (rate of congenital infection of 1%). Among infected infants at birth, the number of severely infected foetuses would be approximately 75, the number of infants with severe sequelae would be 480, 675 approximately would present with hearing loss and the number of asymptomatic infants would be 6270. Five different preventive methods for congenital CMV infection are possible: (1) Routine CMV screening at the beginning of pregnancy for primary prevention. (2) Secondary prevention by antenatal diagnosis of congenital CMV infection complications. (3) Tertiary prevention by serological testing during pregnancy. (4) Tertiary prevention by serological screening at birth. (5) Tertiary prevention: Hearing loss screening at birth. The aims of this review are to define the advantages and disadvantages of these different methods of CMV screening during pregnancy and to determine if the current available information would make systematic testing acceptable.
Assuntos
Infecções por Citomegalovirus/prevenção & controle , Complicações Infecciosas na Gravidez/prevenção & controle , Diagnóstico Pré-Natal , Infecções por Citomegalovirus/congênito , Infecções por Citomegalovirus/epidemiologia , Feminino , França/epidemiologia , Humanos , Recém-Nascido , Programas de Rastreamento , Triagem Neonatal , Gravidez , Complicações Infecciosas na Gravidez/epidemiologiaRESUMO
OBJECTIVE: The aim of the study was to evaluate human papillomavirus (HPV) testing during the follow-up of patients after conization by loop electrosurgical excision for high-grade squamous intraepithelial lesion. METHODS: A prospective study was conducted on 205 patients who underwent conization for high-grade squamous intraepithelial lesion (CIN 2 or 3). Loop electrosurgical excision procedure (LEEP) was used in all cases. High-risk HPV testing was realized by the Hybrid Capture II system before and 3 months after conization. RESULTS: Of the 205 patients, 193 (94.1%) were positive for the HPV test before conization. Seventy-one were HPV positive after conization (34.6%). The margins were positive in 36.1%. Residual disease was observed in 27 cases (13.2%). Four patients (2%) developed a recurrence after a mean follow-up of 18.1 months (+/-12). There was no correlation between pretreatment HPV testing and the residual disease or recurrence. Patients with positive margins were significantly more likely to have residual disease than those with negative margins (P < 0.0001). Residual disease was more likely to occur when the posttreatment HPV test was positive (P < 10(-7)). All recurrences were observed in patients with a positive posttreatment HPV test (P < 0.05). Residual disease and recurrence were correctly predicted with a sensitivity of 81 and 100%, respectively, and a negative predictive value of 96 and 100%. CONCLUSION: Posttreatment HPV testing could be useful in the follow-up of patients after conization. In case of negative posttreatment HPV testing, the frequency of follow-up could be reduced, particularly in those patients with free margins.
Assuntos
Conização/métodos , Eletrocirurgia/métodos , Papillomaviridae/genética , Displasia do Colo do Útero/cirurgia , Displasia do Colo do Útero/virologia , Neoplasias do Colo do Útero/cirurgia , Neoplasias do Colo do Útero/virologia , Adolescente , Adulto , Idoso , DNA Viral/análise , Feminino , Humanos , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/virologia , Neoplasia Residual , Valor Preditivo dos Testes , Estudos Prospectivos , Neoplasias do Colo do Útero/patologia , Displasia do Colo do Útero/patologiaRESUMO
We studied the role of viruses and atypical bacteria in children hospitalized with exacerbated asthma by a prospective study of children with acute asthma admitted to the Department of Pediatrics in Lille, and to 15 hospitals in the Nord-Pas de Calais region, from October 1, 1998-June 30, 1999. We included children aged 2-16 years with active asthma, defined as three or more recurrent episodes of reversible wheezing. The severity of asthma and of asthmatic exacerbations was recorded. Immunofluorescence assays (IFA) on nasopharyngeal secretions (NPS), serological tests, or both, were used for detection of influenza virus, respiratory syncytial virus (RSV), adenovirus, parainfluenza virus, and coronavirus. Polymerase chain reaction (PCR) assays on NPS were used for rhinovirus and enterovirus. Serological tests for Chlamydia pneumoniae and Mycoplasma pneumoniae were performed. A control group of asymptomatic asthmatic outpatients was examined for respiratory viruses (using IFA and PCR). Eighty-two symptomatic children (mean age, 7.9 years) were examined. Viruses were detected in 38% (enterovirus, 15.8%; rhinovirus, 12%; RSV, 7.3%). Serological tests for atypical bacteria were positive in 10% of patients (C. pneumoniae, 5%; M. pneumoniae, 5%). Among the 27 control subjects (mean age, 7.9 years), one PCR was positive for enterovirus. There was no correlation between severity of chronic asthma or asthmatic exacerbations and the diagnosis of infection. Atypical bacterial pathogen infections were linked with prolonged asthmatic symptoms. In conclusion, we confirmed the high incidence of viral infection in acute exacerbations of asthma, especially enteroviruses or rhinoviruses. Persistent clinical features were more frequently associated with atypical bacterial infections, suggesting that these infections should be investigated and treated in cases of persistent asthmatic symptoms.
Assuntos
Asma/microbiologia , Asma/virologia , Chlamydophila pneumoniae/isolamento & purificação , Chlamydophila pneumoniae/fisiologia , Hospitalização , Mycoplasma pneumoniae/isolamento & purificação , Mycoplasma pneumoniae/fisiologia , Vírus de RNA/isolamento & purificação , Vírus de RNA/fisiologia , Adolescente , Fatores Etários , Asma/fisiopatologia , Criança , Pré-Escolar , Feminino , França , Humanos , Masculino , Estudos Prospectivos , Índice de Gravidade de DoençaAssuntos
Transplante de Medula Óssea , Transplante de Células-Tronco Hematopoéticas , Síndromes de Imunodeficiência/complicações , Terapia de Imunossupressão , Transplante de Órgãos , Virologia , Viroses/etiologia , Adulto , Fatores Etários , Criança , Infecções por Citomegalovirus/complicações , Infecções por Citomegalovirus/etiologia , Infecções por Citomegalovirus/imunologia , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/etiologia , Infecções por Vírus Epstein-Barr/imunologia , Feminino , França , Infecções por HIV/complicações , Infecções por HIV/etiologia , Infecções por HIV/imunologia , Hepatite C/complicações , Hepatite C/etiologia , Hepatite C/imunologia , Infecções por Herpesviridae/complicações , Infecções por Herpesviridae/etiologia , Infecções por Herpesviridae/imunologia , Humanos , Transplante de Rim , Transplante de Fígado , Masculino , Pessoa de Meia-Idade , Prognóstico , Recidiva , Fatores de Risco , Fatores de Tempo , Doadores de Tecidos , Viroses/complicações , Viroses/imunologiaRESUMO
A Herpes Consensus allows the simultaneous detection of 6 human herpesviruses: herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), human cytomegalovirus (HCMV), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), and human herpes virus 6 (HHV-6). This technique was used first to examine retrospectively 100 DNA extracts from 95 CSF and 5 aqueous fluids, prepared by treatment by saturated NaCl followed by ethanol precipitation (n = 63) or by simple boiling (n = 37) and stored at -80 degrees C, and secondly to test prospectively 38 CSF samples for which two DNA extracts were prepared with commercially available DNA extraction kits. In all cases, the results were compared with those of an "in-house" PCR. Concordant results between both PCR and the Herpes Consensus techniques were obtained in 61 of 63 DNA extracts prepared by treatment by saturated NaCl (97%) and in only 31 of 37 boiled samples (84%). Both commercially available methods of DNA extraction examined appear to be suitable for Herpes Consensus PCR, although they cannot remove completely PCR inhibitors that must be sought in case of negative results. This preliminary study shows that the Herpes Consensus method should be of value for rapid diagnosis of herpesvirus infections on condition that it is performed on purified DNA extracts.
Assuntos
Humor Aquoso/virologia , Líquido Cefalorraquidiano/virologia , DNA Viral/análise , Herpesviridae/isolamento & purificação , Infecções do Sistema Nervoso Central/virologia , Citomegalovirus/genética , Citomegalovirus/isolamento & purificação , Etanol , Infecções Oculares Virais/virologia , Congelamento , Herpesviridae/genética , Infecções por Herpesviridae/virologia , Herpesvirus Humano 1/genética , Herpesvirus Humano 1/isolamento & purificação , Herpesvirus Humano 2/genética , Herpesvirus Humano 2/isolamento & purificação , Herpesvirus Humano 3/genética , Herpesvirus Humano 3/isolamento & purificação , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/isolamento & purificação , Herpesvirus Humano 6/genética , Herpesvirus Humano 6/isolamento & purificação , Humanos , Reação em Cadeia da Polimerase/métodos , Estudos Retrospectivos , Sensibilidade e Especificidade , Cloreto de SódioAssuntos
Vírus de DNA/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Naftalenos/química , Oxigênio/farmacologia , Peróxidos/química , Adenoviridae/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Meios de Cultura , Citomegalovirus/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/crescimento & desenvolvimento , HIV-1/efeitos dos fármacos , Temperatura Alta , Humanos , Hidrólise , Testes de Sensibilidade Microbiana , Oxigênio/química , Oxigênio/toxicidade , Oxigênio SingleteRESUMO
To define the role of enteroviruses and human rhinoviruses as etiological agents in childhood bronchiolitis, clinical aspirates from 84 infants admitted to hospital with symptoms of obstructive bronchiolitis were tested by picornavirus RT-PCR assay, adenovirus PCR assay and classical immunofluorescence antigen detection of common respiratory viral agents. Respiratory syncytial viruses (A&B) were detectable in 45 of 84 (53.6%) nasopharyngeal aspirates from infants with bronchiolitis, whereas coronaviruses, influenza viruses, and parainfluenza viruses were not detectable in the same samples. Adenoviruses were detectable by PCR in 11 of 84 (13.1%) nasopharyngeal swabs. By using a picornavirus RT-PCR assay followed by a differential molecular hybridisation, rhinovirus and enterovirus RNA sequences were detected in 16 of 84 (19%) and in 10 of 84 (11.9%) of the nasopharyngeal swabs tested. Positive human rhinovirus or enterovirus RT-PCR assay, however, was the only evidence of respiratory infection in 8 of 84 (9.5%) and in 7 of 84 (8.33%) of the studied patients. Respiratory syncytial viruses, human rhinoviruses, adenoviruses, and enteroviruses occur in dual infections detected in 18 of 84 (21.4%) respiratory samples tested. The median duration of stay in hospital was not significantly different between the patients demonstrating a single viral infection and those with a dual viral infection (6.22 +/- 2.07 vs. 5. 04 +/- 0.95 days; P > 0.05). In summary, combination of molecular and classical detection assays of common viruses can be used to demonstrate enterovirus and human rhinovirus respiratory infection in childhood bronchiolitis, and provides an improved approach to obtain new insights into concomitant viral respiratory tract infection in infants.
Assuntos
Bronquiolite/virologia , Infecções por Enterovirus/virologia , Enterovirus/isolamento & purificação , Nasofaringe/virologia , Infecções por Picornaviridae/virologia , Rhinovirus/isolamento & purificação , Antígenos Virais/análise , Bronquiolite/diagnóstico , Enterovirus/genética , Enterovirus/imunologia , Feminino , Imunofluorescência , Humanos , Lactente , Masculino , Hibridização de Ácido Nucleico , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rhinovirus/genética , Rhinovirus/imunologia , Análise de Sequência de DNARESUMO
The presence of human papillomavirus (HPV) DNA in 79 cervical specimens obtained from 70 patients was studied by using a molecular hybridization technique performed in tube. The results were compared to those of the cytological and histological studies. The molecular hybridization technique in tube (Hybrid Capture I) detects two groups of HPV types. One group is highly associated with the development of cancer (types 16, 18, 31, 33, 35, 45, 51, 52, 56) whereas the second group (types 6, 11, 42, 43, 44) is not. Among 42 patients with cervical lesions before any treatment, high risk DNA of HPV was found in 50% of those with low grade cytology and 90% with high grade cytology. In total, 32 out of the 42 patients (76%) who presented histological lesions, were actually infected by HPV. Samples were obtained before and after treatment from 9 patients. Seven out of 9 presented high grade cervical intraepithelial neoplasia (CIN) and 2 other patients had low grade CIN. HPV DNA was not detected in any of the patients after treatment. Detection of HPV DNA by molecular hybridization in tube is simple, sensitive, standardized, inexpensive and is well adapted to screening programs. It can be used in complement of the cytological diagnosis, in the surveillance of equivocal cytological abnormalities, and in the follow-up of treated patients.
Assuntos
DNA Viral/análise , Papillomaviridae/classificação , Infecções por Papillomavirus/diagnóstico , Infecções Tumorais por Vírus/diagnóstico , Displasia do Colo do Útero/virologia , Adenocarcinoma/patologia , Adenocarcinoma/terapia , Adenocarcinoma/virologia , Idoso , Condiloma Acuminado/patologia , Condiloma Acuminado/terapia , Condiloma Acuminado/virologia , Feminino , Seguimentos , Doenças dos Genitais Femininos/patologia , Doenças dos Genitais Femininos/terapia , Doenças dos Genitais Femininos/virologia , Humanos , Programas de Rastreamento , Hibridização de Ácido Nucleico , Papillomaviridae/genética , Infecções por Papillomavirus/patologia , Sensibilidade e Especificidade , Infecções Tumorais por Vírus/patologia , Displasia do Colo do Útero/patologia , Displasia do Colo do Útero/terapia , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/terapia , Neoplasias do Colo do Útero/virologiaRESUMO
BACKGROUND: Human polyomavirus JC (JCV) induces human progressive multifocal leukoencephalopathy (PML) in patients with AIDS. Peripheral blood mononuclear cells (PBMC) of HIV-1-positive immunocompetent and immunocompromised patients can harbour JCV genome, but their precise role in JCV latency or reactivation status before the onset of PML remains hypothetical. OBJECTIVES: To assess JCV latency or reactivation status in PBMC of HIV-1-positive immunocompromised patients without PML. DESIGN: A group of 82 HIV-1-positive immunocompromised patients who did not have PML were compared with 10 patients with AIDS and PML and with 69 HIV-1-positive immunocompetent patients without PML. METHODS: DNA and total RNA were extracted from PBMC. The presence of JCV DNA was demonstrated by a semi-nested polymerase chain reaction (PCR). By using primer pairs specific for an early gene,T, and a late gene, VP1, the expression of both early and late gene mRNA in PBMC could be identified using reverse transcriptase (RT) PCR. RESULTS: JCV DNA was detected by PCR in 17.4% of 69 HIV-1-positive immunocompetent patients, in 23.2% of 82 HIV-1-positive immunocompromised patients, and in 60% of 10 patients with AIDS and PML. No correlation could be drawn between the detection of JCV DNA in the PBMC and the clinical or biological status of the HIV-1-positive patients. By using RT-PCR procedures, no expression of JCV early and late mRNA in PBMC was found in any patients. CONCLUSIONS: JCV DNA is detectable in the PBMC of 20.5% of 151 HIV-1-infected patients independently of the CDC (Centers for Disease Control and Prevention) stages of the infection. Moreover, our results suggest that active replication of JCV in PBMC appears to be absent or at least a very rare event in HIV-1-positive immunocompromised patients with and without PML.