Biofortification of mungbean (Vigna radiata L. (Wilczek)) with boron, zinc and iron alters its grain yield and nutrition.

Mungbean [Vigna radiata L. (Wilczek)] is considered as an extremely nutritious crop possessing a high level of micronutrients, but their low bioavailability in the crop leads to micronutrient malnutrition in humans. Therefore, the present study was conducted to investigate the potential of nutrients viz. boron (B), zinc (Zn) and iron (Fe) biofortification on productivity, nutrient concentration and uptake as well as the economics of mungbean cultivation. In the experiment, the various combinations of RDF with ZnSO4.7H2O (0.5%), FeSO4.7H2O (0.5%) and borax (0.1%) were applied to mungbean variety ML 2056. The combined foliar application of Zn, Fe and B was highly efficient in increasing the yield of grain as well as straw in mungbean exhibiting maximum values i.e. 944 kg ha-1 and 6133 kg ha-1, respectively. Similar results for B, Zn and Fe concentration in grain (27.3 mg kg-1, 35.7 mg kg-1 and 187.1 mg kg-1, respectively) and straw (21.1 mg kg-1, 18.6 mg kg-1 and 376.1 mg kg-1, respectively) of mungbean were observed. Also, uptake of Zn and Fe by grain (31.3 g ha-1 and 164.4 g ha-1, respectively), as well as straw (113.7 g ha-1 and 2295.0 g ha-1, respectively), was maximum for the above treatment. Whereas, the B uptake was found to enhance significantly through the combined application of B, Zn and Fe, where the values 24.0 g ha-1 and 128.7 g ha-1 corresponded to grain and straw, respectively. Thus, combined use of ZnSO4.7H2O (0.5%) + FeSO4.7H2O (0.5%) and borax (0.1%) significantly improved the yield outcomes, the concentration of B, Zn and Fe, uptake and economic returns of mungbean cultivation to alleviate the B, Zn and Fe deficiency.

Biofortification of oil quality, yield, and nutrient uptake in Indian mustard (Brassica juncea L.) by foliar application of boron and nitrogen.

Indian mustard (Brassica juncea L.) is an essential oilseed crop that offers important nutrients to human beings. However, the concurrent micronutrient deficiencies including boron (B), sulfur (S), and nitrogen (N) could pose a significant threat to public health. Therefore, this study was conducted at the Punjab Agricultural University, Ludhiana, with nine treatments, i.e., T1-Control (recommended NPK only), T2- borax (0.5%) at flowering, T3-borax (1.0%) at flowering,T4- borax (0.5%) + urea (1.0%) at flowering,T5-borax (1.0%) + urea (1.0%) at flowering, T6-borax (0.5%) at flowering + capsule formation, T7-borax (1.0%) at flowering + capsule formation, T8-borax (0.5%) + urea (1.0%) at flowering + capsule formation, T9-borax (1.0%) + urea (1.0%) at flowering + Capsule formation, replicated three times in a randomized block design for 2 years (2020-2021 and 2021-2022). The foliar application of borax (1.0%) + urea (1.0%) at the flowering and capsule formation stage (treatment T9) was highly efficient in increasing food quality parameters such as crude fiber, total soluble solids (TSS), and protein content with maximum values of 3.77, 24.9, and 27.53%, respectively. Also, maximum yields of seed as well as stover for treatment T9 were 1.376 and 6.625 kg ha-1, respectively. Similarly, the results for B, S, and N concentrations in seed (27.71 mg kg-1, 17.69 mg kg-1, and 2.35%), as well as stover (25.92 mg kg-1, 17.31 mg kg-1, and 0.33%), were maximum in treatment T9. Also, B, S, and N uptake by seed (38.18 g ha-1, 24.40 g ha-1, and 32.05 Kg ha-1) and stover (172.55 g ha-1, 115.44 g ha-1, and 21.99 Kg ha-1) were maximum for the treatment T9 involving borax (1.0%) + urea (1.0%) at the flowering and capsule formation stage. Whereas, the concentration and uptake decreased in the treatments involving the sole application of borax and urea. Therefore, the application of borax (1.0%) and urea (1.0%) at the flowering and capsule formation stage significantly improved the quality parameters, seed and stover yield, nutrient concentration, and uptake over control and could be used to alleviate the B, S, and N deficiency in Indian mustard.

Interactive Effects of Molybdenum, Zinc and Iron on the Grain Yield, Quality, and Nodulation of Cowpea (Vignaunguiculata (L.) Walp.) in North-Western India.

Micronutrient deficiency is a major constraint for the growth, yield and nutritional quality of cowpea which results in nutritional disorders in humans. Micronutrients including molybdenum (Mo), iron (Fe) and zinc (Zn) play a pivotal role in crop nutrition, and their role in different metabolic processes in crops has been highlighted. In order to increase the nutritional quality of cowpea, a field experiment was conducted for two years in which the effect of Mo along with iron (Fe) and zinc (Zn) on productivity, nitrogen and micronutrient uptake, root length and the number of nodules in cowpea cultivation was investigated. It was found that the foliar application of Fe and Zn and their interaction with Mo application through seed priming as well as soil application displayed increased yield, nutrient concentration, uptake and growth parameters which helped to enhance the nutritional quality of cowpea for consumption by the human population. The results of the above experiments revealed that among all the treatments, the soil application of Mo combined with the foliar application of 0.5% each of FeSO4·7H2O and ZnSO4·7H2O (M2F3 treatment) enhanced the grain and stover yield of cowpea, exhibiting maximum values of 1402 and 6104.7 kg ha-1, respectively. Again, the M2F3 treatment resulted in higher Zn, Fe and Mo concentrations in the grain (17.07, 109.3 and 30.26 mg kg-1, respectively) and stover (17.99, 132.7 and 31.22 mg kg-1, respectively) of cowpea. Uptake of Zn, Fe and Mo by the grain (25.23, 153.3 and 42.46 g ha-1, respectively) as well as the stover (104.2, 809.9 and 190.6 g ha-1, respectively) was found to be maximum for the M2F3 treatment. The root length (30.5 cm), number of nodules per plant (73.0) and N uptake in grain and stover (55.39 and 46.15 kg ha-1) were also higher for this treatment. Overall, soil application of Mo along with the foliar application of FeSO4·7H2O (0.5%) and ZnSO4·7H2O (0.5%) significantly improved yield outcomes, concentration, uptake, root length, nodules plant-1 and N uptake of cowpea to alleviate the micronutrient deficiency.

Cadmium phytoremediation potential of Brassica genotypes grown in Cd spiked Loamy sand soils: Accumulation and tolerance.

Phytoremediation acts as an efficient methodology for management of toxic elements spiked soils. The accumulation and tolerance potential of hyper-accumulator plants for toxic elements act as an index for in-situ removal of toxic elements. Extraction of cadmium (Cd) through its accumulation in harvestable parts of plants has attracted attention as the economic and environment friendly technique. Brassica genotypes have greater potential to accumulate Cd when grown in Cd spiked soils. Therefore, for evaluation of comparative efficiency of three Brassica genotypes (B. juncea, B. campestris and B. napus) in phytoremediation of Cd spiked soils, a pot study was carried out in Cd contaminated soil with 6 levels as 0, 5, 10, 20, 40, and 80 mg kg-1 soil. Results indicated that dry biomass production of Brassica genotypes declined with the enhanced Cd contamination in soil. The reduction in grain and shoot yield varied from 2.87 to 1.85 and 11.85 to 8.00 g pot-1 with increased Cd contamination from 5 to 80 mg kg-1 soil. Similarly, increased levels of Cd contamination resulted in enhanced concentration and accumulation in grains as well as shoots of all Brassica genotypes. Among Brassica genotypes, B. juncea recorded the highest production of dry biomass (12.8 g pot-1), Cd accumulation (736.0 µg pot-1). Also, the bioaccumulation coefficient and tolerance index indicated that B. juncea is the most tolerant genotype to Cd contamination in soil. Therefore, B. juncea could act as the most potential genotypes for decontamination of Cd spiked soils by preventing its entry into food chain.

Characterization and optimization of spray dried iron and zinc nanoencapsules based on potato starch and maltodextrin.

In this study, spray drying mechanism was used to optimize the nanoencapsulation of iron and zinc using potato starch at constant proportion and variable maltodextrin and core material to keep the total solids of spray drier feed solution at 10, 20, 30 and 40% levels. Results exhibited that stable nanoencapsulates of iron and zinc were formed at 30% level with maximum in-vitro bioavailability and encapsulation efficiency of 90.68 and 89.36%, respectively. At this level the particle size and zeta potential of iron and zinc nanoencapsulates were 340.9 and 354.5 nm; 0.372 and 11.40 mV, respectively. Further, FTIR and XRD analysis of Fe 30 and Zn 30 nanoencapsulates exhibited that core material was successfully encapsulated by existence of functional groups and a semi-crystalline structure, respectively. Thus, study suggests the suitability of cheap carriers like potato starch and maltodextrin in successful encapsulation of iron and zinc indicating application potentiality in food fortification.

Effect of cadmium and ethylenediamine tetraacetic acid supplementation on cadmium accumulation by roots of Brassica species in Cd spiked soil.

Cadmium (Cd) metal extraction through efficient plant roots has attracted much attention as this methodology is environment-friendly and cost-effective. Brassica species are well known for their tolerance towards high Cd concentration in contaminated soils. The tolerance ability may vary among species; hence the assessment of this variability is mandatory for selecting Brassica species. For this purpose, a greenhouse pot experiment was carried out using three Brassica species (Brassica juncea L., Brassica campestris L., and Brassica napus L.). To evaluate the effect of chelating agent ethylenediamine tetraacetic acid (EDTA) on Cd uptake, EDTA (0, 1, and 2 g kg-1 soil) was supplemented along with Cd (0, 5, 10, 20, 40, and 80 mg kg-1 soil). Among different species, B. juncea possessed the highest root dry biomass and lowest root Cd concentration in untreated soil. Overall root dry biomass of all tested Brassica species reduced on increasing Cd and EDTA levels. The trend was appeared to be related to an increase in root Cd concentration on the supplementation of EDTA that formed a complex with the target metal contaminate and resulted in vacuolar sequestration. Roots of B. juncea showed maximum Cd accumulation and highest values at Cd and EDTA levels up to 20 mg kg-1 and 1 g kg-1 soil due to the combined effect of root biomass and Cd concentration in roots. Thus, present findings inferred that Cd and EDTA supplementation might prove as a feasible strategy to improve remediation of Cd-polluted soil using B. juncea as an efficient Cd accumulator.

Enrichment of Zinc and Iron Micronutrients in Lentil (Lens culinaris Medik.) through Biofortification.

Biofortification of pulse crops with Zn and Fe is a viable approach to combat their widespread deficiencies in humans. Lentil (Lens culinaris Medik.) is a widely consumed edible crop possessing a high level of Zn and Fe micronutrients. Thus, the present study was conducted to examine the influence of foliar application of Zn and Fe on productivity, concentration, uptake and the economics of lentil cultivation (LL 931). For this, different treatment combinations of ZnSO4·7H2O (0.5%) and FeSO4·7H2O (0.5%), along with the recommended dose of fertilizer (RDF), were applied to the lentil. The results of study reported that the combined foliar application of ZnSO4·7H2O (0.5%) + FeSO4·7H2O (0.5%) at pre-flowering (S1) and pod formation (S2) stages was most effective in enhancing grain and straw yield, Zn and Fe concentration, and uptake. However, the outcome of this treatment was statistically on par with the results obtained under the treatment ZnSO4·7H2O (0.5%) + FeSO4·7H2O (0.5%) at S1 stage. A single spray of ZnSO4·7H2O (0.5%) + FeSO4·7H2O (0.5%) at S1 stage enhanced the grain and straw yield up to 39.6% and 51.8%, respectively. Similarly, Zn and Fe concentrations showed enhancement in grain (10.9% and 20.4%, respectively) and straw (27.5% and 27.6% respectively) of the lentil. The increase in Zn and Fe uptake by grain was 54.8% and 68.0%, respectively, whereas uptake by straw was 93.6% and 93.7%, respectively. Also the benefit:cost was the highest (1.96) with application of ZnSO4·7H2O (0.5%) + FeSO4·7H2O (0.5%) at S1 stage. Conclusively, the combined use of ZnSO4·7H2O (0.5%) + FeSO4·7H2O (0.5%) at S1 stage can contribute significantly towards yield, Zn and Fe concentration, as well as uptake and the economic returns of lentil to remediate the Zn and Fe deficiency.

Phytoavailability and human risk assessment of heavy metals in soils and food crops around Sutlej river, India.

We assessed the phytoavailability of heavy metals in wheat and rice grown on soils along flood plains of Sutlej river (India) and its effects on human health. Surface soil samples along with wheat (pre-monsoon season) and rice (post-monsoon season) grain samples were collected during the years 2017 and 2018. Soil samples were analysed for total and DTPA-extractable metals and, wheat and rice grains samples for total metals. There were no significant (p < 0.05) changes in total metal concentration during both the years, but the concentration of DTPA-extractable metals was higher during pre-monsoon than post-monsoon season. The concentration of Pb, Cd, and Co in wheat grains, whereas the concentration of Cr, Co, Pb and Cd in rice grains surpassed their safe limits. Both DTPA-extractable metals (water soluble plus exchangeable) and non-exchangeable fractions explained more than 80% of the variations in metal uptake by plants. The bioaccumulation factor for DTPA-extractable metals (BCF) was above one, but Pollution Load Index for these metals (PLI) was less than one. The carcinogenic risks due to ingestion of wheat and rice grains were higher for Cd. The BAF, PLI and health risks were higher along transboundary of the river. These results suggest that exchangeable and non-exchangeable fractions are contributing towards metals uptake by wheat and rice along flood plain soils of the Sutlej river and the present study may act as a model for carrying out similar studies to find out the risks of heavy metals and it effect on human health in future.

Impact assessment of metal contamination in surface water of Sutlej River (India) on human health risks.

The Sutlej river in Indian Punjab is getting contaminated by industrial waste, sewage, and agricultural runoff. Most of the previous studies on metal contamination of water are mainly confined to the small stretch of river. Therefore, a systematic study was carried out to assess the spatial and temporal variability of metal contamination in water from the entry point of Sutlej River in Indian Punjab to its tail end when it leaves the country. The likelihood of cancer risk was also estimated though human health risk assessment. The water samples (between 76 and 91) were collected from Sutlej river during pre- (April) and post-monsoon (September-October) seasons of the years 2017 and 2018. There samples were analyzed for 10 metals (Zn, Cu, Fe, Mn, Ni, Cd, Pb, Co, Cr and As) using Atomic Absorption Spectrophotometer. Results showed that metal concentration in water was higher along the transboundary during both the seasons. The concentration of metals in water was higher in pre-monsoon season than post-monsoon season. Multivariate analysis in different seasons showed that biophysical variables and social drivers (including anthropogenic activities) have great influence on the concentration of metals. Spatial distribution of all the metals in water and heavy metal pollution index showed that metal contamination was higher along the transboundary followed by the area from the confluence of contaminated drain with river to transboundary along with localized spots in the river flowing in Indian Punjab. The likelihood of cancer risk due to ingestion of metals through water was in the order: Cd > Ni > Cr > As and the risk is higher in the areas along the transboundary. These results are useful for formulating the action plan to improve the water quality of Sutlej river and its environs including water-soil-plant continuum which affect human health.