Broad-specificity time-resolved fluorescent immunochromatographic strip for simultaneous detection of various organophosphorus pesticides based on indirect probe strategy.

The massive use of organophosphorus pesticides (OPs) poses a great threat to food safety, human health and environmental protection. As there are many kinds of pesticides, their detection is facing a severe challenge. The simultaneous detection of multiple organophosphorus pesticides in one test is a problem to be solved at present. In this paper, a time-resolved fluorescent immunochromatographic (TRFIA) strip is prepared by using broad-specificity antibodies (Abs) of OPs as the recognition element. Abs were connected to europium oxide latex microspheres using sheep anti-mouse antibodies (SaMIgG) to form an indirect probe. This strategy could effectively realize signal amplification, and could save the amount and protect the activity of Abs. After the detection, the color change of the test line (T-line) was observed to make qualitative judgment under UV-light (365 nm). Then, the images of the positive sample were analyzed by using ImageJ to complete the quantitative detection. Under optimal construction and operating conditions, the limit of detection of the strip could reach 0.53 ng g-1. And the TRFIA strip performed well in the additive test of vegetable samples. It is inexpensive to prepare, convenient to carry, and easy to operate. More importantly, it improves the detection efficiency and meets the needs of rapid field testing of a large number of samples.

A novel label-free electrochemiluminescence aptasensor using a tetrahedral DNA nanostructure as a scaffold for ultrasensitive detection of organophosphorus pesticides in a luminol-H2O2 system.

In this work, a new type of Au-tetrahedral DNA nanostructure (Au-TDN) was originally proposed and successfully applied in an electrochemiluminescence aptasensor to detect organophosphorus pesticides (Ops). The aptamers modified with -SH could be covalently bonded with gold nanoparticles (AuNPs) to form a tetrahedron structure, and there were independent probes at each vertex of the tetrahedron, which could increase the probability of specific binding with Ops. The originally designed structure could not only maintain a stable tetrahedral configuration, but also combined with the target to improve the sensitivity of the sensor. Meanwhile, silver nanoparticles (AgNPs) could catalyze the chemical reaction between luminol and H2O2 to generate a variety of intermediates called reactive oxygen species (ROS) for signal enhancement. Factors that had important influences on the aptasensor, such as the concentration of Au-TDN, the incubation time, and the pH value of the buffer, were optimized in this trial. According to the final results, the limit of detection (LOD) of 3 pg mL-1 (S/N = 3) for methyl parathion, the LOD of 0.3 pg mL-1 (S/N = 3) for parathion and the LOD of 0.03 pg mL-1 (S/N = 3) for phoxim were obtained, respectively. Moreover, the novel tetrahedral structure could be replaced by different types of aptamers to expand its application range and lay a foundation for the development of portable rapid detection devices for pesticide residues.