[Localization of gestational age reference table and its application in prenatal screening].

Objective: To establish a fetal biparietal diameter (BPD)-gestational age formula based on the data of pregnant women from Xiaoshan District of Hangzhou, and to evaluate its application in prenatal screening. Methods: Data of 3500 pregnant women with gestational age between 15 weeks and 19 weeks+6 receiving prenatal screening in Xiaoshan Hospital during May 2014 and May 2015 were collected. BPDs were used to establish a localized BPD-gestational age formula. The localized formula was used to evaluate the prenatal screening risks in 1759 pregnant women with irregular menstrual cycles or uncertain last menstrual period (LMP) in Xiaoshan District, and the results were compared with those calculated using formula in LifeCycle 4.0. Results: With localized formula, the total positive rate of Down syndrome, trisomy 18 syndrome and deformity of neural tube was decreased from 6.96% to 5.85% ( P<0.05), in which the positive rate of Down syndrome decreased ( P<0.05), that of deformity of neural tube increased ( P<0.05), and that of trisomy 18 syndrome remained the same ( P>0.05). The median MoMs of free-hCG ß and α-fetoprotein calculated using localized formula were significantly different from those calculated using the formula in LifeCycle 4.0 (all P<0.05), and the former ones were more closer to 1. For women of fetus diagnosed with the above diseases, the positive rate calculated using localized formula was almost the same as that calculated using the formula in LifeCycle 4.0. Conclusion: BPD-gestational age formula should be localized based on the statistical analysis of the local population, which will help to reduce the false positive rate, and make the results more accurate and reliable in prenatal screening.

[Determination of 14 elements in the body fluid and hair of lung cancer patients by microwave digestion with ICP-MS].

BACKGROUND AND OBJECTIVE: The trace element contents in the body fluid and hair are the important monitoring indicators for many diseases. The analysis of the trace element contents in the samples of lung cancer patients is helpful to the early diagnosis and treatment effectiveness evaluation to the patients. The aim of this study is to develop an ICPMS method for the determination of Cr, Fe, Mn, Al, Cd, Cu, Zn, Ni, Se, Pb, Ca, Mg, Sr, P in the body fluid and hair of lung cancer patients. METHODS: Samples of body fluid and hair from lung cancer patients were digested with microwave and 14 trace elements were determined with inductively coupled plasma mass spectrometry RESULTS: GBW09101 standard reference material of human hair was used to validate the accuracy of the established method, and the results indicated that there is no obvious difference between the measured values and the references values. Forty-eight samples from 16 lung cancer patients were analyzed with the established method, and several generalizations were discovered. CONCLUSION: The established method can be used for the multielement simultaneous determination of the samples of lung cancer patients, which are helpful to the diagnosis and treatment of the lung cancer.