[Laparoscopic circular stapled gastrointestinal anastomosis using novel device of sealed cap access after total laparoscopic gastrectomy].

Intracorporeal classic gastrointestinal anastomosis using circular stapler in totally laparoscopic gastrectomy (TLG) for gastric cancer requires intracorporeal anvil placement and suitable access for introduction of the circular stapler to the abdominal cavity without gas leak. The novel techniques for anvil placement have been updated, but there is no progress for proper access for circular stapler. In the study, intracorporeal circular-stapled gastrointestinal anastomosis were successfully accomplished using a novel device of sealed cap access with a central hole (WLB-60/70-60/100, Wuhan Widerep Medical Instrument Co.,Ltd, China) customized to the incision protection retractor for the simple and accessible introduction of the circular stapler and anvil under the optimal maintenance of pneumoperitoneum pressure in TLG. In these 3 cases, there was no gas leakage and the pneumoperitoneum was well maintained when performing the gastrointestinal anastomosis, and there was no transition to laparotomy or other anastomosis techniques. The result suggests that the sealed cap access could be a novel choice for introduction of the circular stapler to the abdominal cavity in order to obtain laparoscopic circular-stapled gastroin-testinal anastomosis in TLG.

Decreased expression of miR-1254 is associated with cancer aggressiveness and predicts poor outcome in cervical cancer.

OBJECTIVE: MiR-1254 has been demonstrated to play a critical role in the progression of several types of cancer, but its role in cervical cancer remains largely unclear. The aim of the present study was to examine the expression pattern of miR-1254, and its clinical significance in cervical cancer. PATIENTS AND METHODS: RT-PCR was performed to evaluate miR-1254 levels in 181 pairs of cervical cancer specimens and adjacent noncancerous tissues. The correlation between the miR-1254 levels and the clinicopathological factors of the glioma patients was analyzed. The Kaplan-Meier method was applied to analyze overall survival (OS) and recurrence-free survival (RFS). A Cox proportional hazards regression analysis was used for multivariate analyses of prognostic values. RESULTS: Our results showed that miR-1254 expression was significantly down-regulated in cervical cancer tissues compared with adjacent non-tumor tissues (p<0.01). The level of miR-1254 was observed to be negatively associated with lymphatic invasion (p=0.004), distant metastasis (p=0.009), and FIGO stage (p = 0.002). More importantly, cervical cancer patients with lower miR-1254 expression had significantly poorer RFS (p=0.002) and OS (p=0.004). Moreover, in a multivariate Cox model, we found that miR-1254 expression was an independent prognostic factor for both RFS and OS in cervical cancer (p=0.001 and 0.004, respectively). CONCLUSIONS: The current results demonstrated that low miR-1254 expression was associated with poor outcomes in patients with cervical cancer, indicating miR-1254 could be a potential prognostic marker for cervical cancer patients.

[Analysis of clinical characteristics and treatment outcome of atypical early stage malignant laryngeal tumor].

Objective:To observe the clinical characteristics of 22 cases of malignant laryngeal tumor manifested as smooth mass, and explore the diagnosis, pathological types and treatment of the disease.Method:Manifested as smooth mass, 22 cases diagnosed as malignant or borderline tumor by biopsy were collected to summarize the characteristics of pathology, treatment and prognosis. Result:The sex ratio was 13∶9. Eleven cases were diagnosed as malignant tumors of epithelial or mesenchymal origin, 7 cases of tumors of lymphoid hematopoietic system, 4 cases of borderline tumor.Thirteen patients received surgical treatment, and recurrence was not found in postoperative follow-up.Nine patients received radiotherapy and/or chemotherapy, 7 of them were in remission while 2 cases presented progression and metastasis of cervical lymph nodes. Conclusion:Because of lacking typical clinical manifestations in early stage,smooth mass of larynx often easily lead to misdiagnosis.If found and operated early,the prognosis can be satisfied,but the treatment outcome of lymphoma is poor.

[P28GANK is a novel marker for prognosis and therapeutic target in gastric cancer].

P28/PSMD10, a regulatory complex of the human 26S proteasome, plays a critical role in tumor genesis. This study was designed to clarify the clinical significance of p28GANK in gastric cancer. In order to demonstrate the importance of p28GANK expression for the prognosis of gastric cancer, p28GANK expression in 124 paired cases of gastric cancer and noncancerous regions and immortal gastric epithelial cell GES-1 and 5 human gastric cancer cell lines was analyzed by RT-PCR in real-time. MTT was used to observe the effect of P28GANK on cell growth. P28GANK expression was higher in gastric cancer tissues than in corresponding normal tissues (p = 0.0033), and patients comprising the group with p28GANK high expression had a poorer overall survival rate than those from the low expression group (p = 0.0037). Further, the results of multivariate analysis demonstrated that the high p28GANK expression was an independent prognostic factor of gastric cancer process. P28GANK expression was also up-regulated in five gastric cancer cell lines. As it has been shown by in vitro proliferation assay, p28GANK expression correlated with tumor growth. On the base results of present study one can suggests the p28GANK being useful as a predictive marker for patient prognosis and a novel therapeutic target for gastric cancer.

Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver.

Human hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. In this work, we report on a comprehensive characterization of gene expression profiles of hepatitis B virus-positive HCC through the generation of a large set of 5'-read expressed sequence tag (EST) clusters (11,065 in total) from HCC and noncancerous liver samples, which then were applied to a cDNA microarray system containing 12,393 genes/ESTs and to comparison with a public database. The commercial cDNA microarray, which contains 1,176 known genes related to oncogenesis, was used also for profiling gene expression. Integrated data from the above approaches identified 2,253 genes/ESTs as candidates with differential expression. A number of genes related to oncogenesis and hepatic function/differentiation were selected for further semiquantitative reverse transcriptase-PCR analysis in 29 paired HCC/noncancerous liver samples. Many genes involved in cell cycle regulation such as cyclins, cyclin-dependent kinases, and cell cycle negative regulators were deregulated in most patients with HCC. Aberrant expression of the Wnt-beta-catenin pathway and enzymes for DNA replication also could contribute to the pathogenesis of HCC. The alteration of transcription levels was noted in a large number of genes implicated in metabolism, whereas a profile change of others might represent a status of dedifferentiation of the malignant hepatocytes, both considered as potential markers of diagnostic value. Notably, the altered transcriptome profiles in HCC could be correlated to a number of chromosome regions with amplification or loss of heterozygosity, providing one of the underlying causes of the transcription anomaly of HCC.