RESUMO
BACKGROUND: The aim of this work was to simultaneously use multiplex ligation-dependent probe amplification (MLPA) assay and flow cytometric DNA ploidy analysis (FPA) to detect aneuploidy in patients with newly diagnosed acute leukemia. METHODS: MLPA assay and propidium iodide FPA were used to test samples from 53 consecutive patients with newly diagnosed acute leukemia referred to our laboratory for immunophenotyping. Results were compared by nonparametric statistics. RESULTS: The combined use of both methods significantly increased the rate of detection of aneuploidy as compared to that obtained by each method alone. The limitations of one method are somehow countervailed by the other and vice versa. CONCLUSIONS: MPLA and FPA yield different yet complementary information concerning aneuploidy in acute leukemia. The simultaneous use of both methods might be recommended in the clinical setting. © 2017 International Clinical Cytometry Society.
Assuntos
DNA/genética , Leucemia Mieloide Aguda/genética , Aneuploidia , Feminino , Citometria de Fluxo/métodos , Humanos , Imunofenotipagem/métodos , Masculino , Reação em Cadeia da Polimerase Multiplex/métodos , PloidiasRESUMO
Flow cytometric analysis of cell suspensions involves the sequential 'registration' of intrinsic and extrinsic parameters of thousands of cells in list mode files. Thus, it is almost irresistible to describe phenomena in numerical terms or by 'ratios' that have the appearance of 'accuracy' due to the presence of numbers obtained from thousands of cells. The concepts involved in the detection and characterization of B cell lymphoproliferative processes are revisited in this paper by identifying parameters that, when analyzed appropriately, are both necessary and sufficient. The neoplastic process (cluster) can be visualized easily because the parameters that distinguish it form a cluster in multidimensional space that is unique and distinguishable from neighboring clusters that are not of diagnostic interest but serve to provide a background. For B cell neoplasia it is operationally necessary to identify the multidimensional space occupied by a cluster whose kappa:lambda ratio is 100:0 or 0:100. Thus, the concept of kappa:lambda ratio is without meaning and would not detect B cell neoplasia in an unacceptably high number of cases.
Assuntos
Linfócitos B/imunologia , Citometria de Fluxo/métodos , Transtornos Linfoproliferativos/diagnóstico , Transtornos Linfoproliferativos/imunologia , Análise por Conglomerados , Humanos , Cadeias kappa de Imunoglobulina/análise , Cadeias kappa de Imunoglobulina/imunologia , Cadeias lambda de Imunoglobulina/análise , Cadeias lambda de Imunoglobulina/imunologia , Neoplasia Residual/diagnóstico , Neoplasia Residual/imunologiaRESUMO
On May 3 and 4, 2005, the Second Latin American Consensus Conference for the Immunophenotyping of Hematological Malignancies took place in Queretaro, México, with representatives from 10 countries of the region and two external consultants. This document summarizes the major conclusions for which consensus were achieved. Major differences regarding the recommendations from the first conference, which took place 9 years ago, concern the medical indications and the antibody panels for immunophenotyping. The aim of disseminating these guidelines to the international community is based on the potential interest for other countries with similar socioeconomic conditions.
Assuntos
Citometria de Fluxo/métodos , Neoplasias Hematológicas/metabolismo , Imunofenotipagem/métodos , Anticorpos Monoclonais/imunologia , Coleta de Dados , Corantes Fluorescentes/análise , Neoplasias Hematológicas/imunologiaRESUMO
El día 16 de octubre de 1996 se llevó a cabo, en la ciudad de Puebla, México, la primera Conferencia Latinoamericana de Consenso para la Tipificación Inmunológica de Leucemia. En dicha conferencia estuvieron representados 10 países de la región y hubo dos asesores externos: las principales conclusiones a las que se llegaron por acuerdo de los presentes se resumen en esta publicación