Selenium Nanoparticles and Spirulina Alleviate Growth Performance, Hemato-Biochemical, Immune-Related Genes, and Heat Shock Protein in Nile Tilapia (Oreochromis niloticus).

The present investigation aimed to evaluate the influence of selenium nanoparticles (Se-NPs) or/and spirulina (SP) on the growth, immunity, and oxidation resistance of Nile tilapia. Four groups of fish fed diets with Se-NPs or/and SP at 0 g (control), 1 g SP/kg diet (SP), 1 mg Se-NPs/kg diet (Se-NPs), and 1 g SP + 1 mg Se-NPs/kg diet (SP/Se-NPs) for 60 days. Fish fed Se-NPs or/and SP displayed significantly improved weight gain (WG) and decreased feed conversion ratio (P < 0.05). The highest WG has observed in fish fed both Se-NPs and SP, while the specific growth rate was improved by feeding Se-NPs only or both Se-NPs and SP (P < 0.05). Blood albumin was increased significantly with Se-NPs with regard to the control (P < 0.05), while there were no significant differences between fish fed Se-NPs or/and SP. Blood total protein also was improved by feeding Se-NPs only or both Se-NPs and SP (P < 0.05). Further, blood immunoglobulin M was increased by feeding both Se-NPs and SP (P < 0.05), while the differences were insignificantly differing with fish fed only Se-NPs (P > 0.05). The transcription of liver superoxide dismutase (SOD) and tumor necrosis factor-alpha (TNF-α) genes was upregulated significantly by Se-NPs or/and SP (P < 0.05). Interestingly, TNF-α was significantly upregulated by SP when compared to those fed Se-NPs only or both Se-NPs and SP. However, heat shock protein 70 (HSP70) gene transcription was downregulated by Se-NPs or/and SP (P < 0.05). Based on the measured parameters, the mixture of both Se-NPs and SP is highly recommended for the welfare of Nile tilapia.

Production of anticandidal cotton textiles treated with oak gall extract / Producción de tejidos de algodón con actividad anticandidiásica tratados con extracto de agallas de roble

Candida albicans, one of the most dreadful fungal pathogens threatening humans, could not be easily prevented. The anticandidal activity of oak gall extract, Quercus infectoria (QIE), was investigated as a potential natural alternative to synthetic and chemical fungicides. QIE anticandidal potentiality was confirmed using both qualitative and quantitative assays. Cotton textiles were treated with QIE and then evaluated as anticandidal fabrics. QIE-treated textiles had a potent anticandidal activity, which could completely inhibit the inoculated C. albicans cells. The durability of anticandidal activity in QIE-treated textiles almost completely disappeared after the fourth laundering cycle. QIE could be recommended, however, as a potent anticandidal agent for preparing antiseptic solutions and emulsions and as a finishing agent for manufacturing anticandidal disposable diapers and hygienic clothes.

Candida albicans es uno de los patógenos fúngicos más terribles que amenazan la salud humana, y su prevención no resulta sencilla. En este trabajo se investigó la actividad anticandidiásica del extracto de agallas de roble (Quercus infectoria extract; QIE) como una posible alternativa natural a los fungicidas sintéticos y químicos. El potencial anticandidiásico del QIE se confirmó mediante análisis cualitativos y cuantitativos. Se trató tejido de algodón de uso textil con QIE y se lo evaluó como tela anticandidiásica. Se verificó que dichos tejidos exhibían una potente actividad anticandidiásica y que podían inhibir completamente a células de C. albicans inoculadas. La actividad anticandidiásica, sin embargo, desapareció por completo después del cuarto ciclo de lavado. Se concluye que se podría recomendar QIE como un agente anticandidiásico potente para la preparación de soluciones antisépticas y emulsiones, y como un agente de acabado para fabricar pañales desechables y ropa de higiene con propiedades anticandidiásicas.

Potential zoonotic pathways of Salmonella enteritidis in laying farms.

Salmonella Enteritidis is a communicable zoonotic bacterium. The present investigation was done to evaluate the potential occurrence of Salmonella Enteritidis in laying hen farms and its contamination pathways. Samples were collected from 10 laying hen farms located in the Delta of Egypt. Cloacal swabs (n=300), eggshell swabs (n=400), and hand swabs from egg packagers (n=38) were collected. Pools of ovary and oviduct were obtained from 150 hens; all samples were examined for the presence of Salmonella Enteritidis. Results indicated that Salmonella Enteritidis may initially occupy in ovary, oviduct, and cloaca. The risk for eggshell contamination is highly impending from laying hen flocks infected with Salmonella Enteritidis with percentage of >30%. Farms having eggshell contamination percentage of >60% with Salmonella Enteritidis provided a high risk for packagers' hand contamination. Questionnaire of egg packagers specified that seven out of the eight smoker packagers suffered from repeated Salmonellosis. Thus, smoking during egg packing process could be considered as an exposure factor to contract the infection via hand-cigarette-mouth route.