Optimizing health risk assessment for soil trace metals under low-precision sampling conditions: A case study of agricultural soil.

Cost limitations often lead to the adoption of lower precision grids for soil sampling in large-scale areas, potentially causing deviations in the observed trace metal (TM) concentrations from their true values. Therefore, in this study, an enhanced Health Risk Assessment (HRA) model was developed by combining Monte Carlo simulation (MCS) and Empirical Bayesian kriging (EBK), aiming to improve the accuracy of health risk assessment under low-precision sampling conditions. The results showed that the increased sampling scale led to an overestimation of the non-carcinogenic risk for children, resulting in potential risks (the maximum Hazard index value was 1.08 and 1.64 at the 500 and 1000 m sampling scales, respectively). EBK model was suitable for predicting soil TM concentrations at large sampling scale, and the predicted concentrations were closer to the actual value. Furthermore, we found that the improved HRA model by combining EBK and MCS effectively reduced the possibility of over- or under-estimation of risk levels due to the increasing sampling size, and enhanced the accuracy and robustness of risk assessment. This study provides an important methodology support for health risk assessment of soil TMs under data limitation.

Human health risks of heavy metal(loid)s mediated through crop ingestion in a coal mining area in Eastern China.

The heavy metal(loid)s (HMs) in soils can be accumulated by crops grown, which is accompanied by crop ingestion into the human body and then causes harm to human health. Hence, the health risks posed by HMs in three crops for different populations were assessed using Health risk assessment (HRA) model coupled with Monte Carlo simulation. Results revealed that Zn had the highest concentration among three crops; while Ni was the main polluting element in maize and soybean, and As in rice. Non-carcinogenic risk for all populations through rice ingestion was at an "unacceptable" level, and teenagers suffered higher risk than adults and children. All populations through ingestion of three crops might suffer Carcinogenic risk, with the similar order of Total carcinogenic risk (TCR): TCRAdults > TCRTeenagers > TCRChildren. As and Ni were identified as priority control HMs in this study area due to their high contribution rates to health risks. According to the HRA results, the human health risk was associated with crop varieties, HM species, and age groups. Our findings suggest that only limiting the Maximum allowable intake rate is not sufficient to prevent health risks caused by crop HMs, thus more risk precautions are needed.

Human activities contributing to the accumulation of high-risk trace metal(loid)s in soils of China's five major urban agglomerations.

Rapid urbanization has accelerated the accumulation of trace metal(loid)s (TMs) in soils, but the relationship between this accumulation and human activities remains largely unknown. Therefore, based on 775 published literatures (2001-2020), this study aimed to identify the influence of human activities on TM accumulation. Results showed that all soil TM concentrations were higher than their corresponding Chinese soil background values. The pollution risk assessment indicated that the soil TMs in the study area were at moderate levels, and the value of Pollution load index was 2.10. According to the assessment of health risks, the non-carcinogenic risks for adults were at the "Negligible risk" level; while the carcinogenic risk was not negligible for all populations, with children being more susceptible than adults. Meanwhile, six high-risk TMs were identified based on the grading of Contaminating factors (CF ≥ 3) and contribution to health risk (≥ 75%), including four high pollution risk TMs (Cd, Hg, Cu, and Pb) and two high health risk TMs (Cr and As) . In addition, in accordance with the results of the Random forest model, the accumulation of soil high-risk TMs was closely related to influencing factors associated with human activities. The accumulation of Hg and Cr among five major urban agglomerations had the same influencing factors (the number of industrial companies and the amount of industrial wastewater discharge for Hg; the amount of pesticide application and highway mileage for Cr). However, there were significant differences in the factors influencing the accumulation of the other four high-risk TMs (including Cd, As, Cu and Pb), due to the different characteristics of each urban agglomeration. Our results provide new insights into the relationship between human activities and soil TM accumulation.

New insights into health risk assessment on soil trace metal(loid)s: Model improvement and parameter optimization.

Trace metal(loid)s (TMs) in soils may pose potential health risks to humans. Due to model uncertainty and variability of exposure parameters, the traditional health risk assessment (HRA) model may lead to inaccurate risk assessment results. Therefore, this study developed an improved HRA model to assess health risks by combining two-dimensional Monte Carlo simulation (2-D MCS) with a Logistic Chaotic sequence based on published data from 2000 to 2021. The results showed children and adult females were the high-risks populations for Non-carcinogenic risk and Carcinogenic risk, respectively. Meanwhile, children's Ingestion rate (IngR < 160.233 mg/day) and adult females' Skin adherence factor (0.026 mg/(cm2•d) < AF < 0.263 mg/(cm2•d)) were used as recommended exposure to make the health risk within acceptable range. Additionally, when performing risk assessment using actual exposure parameters, priority control TMs were identified, with As being the priority control TM for Southwest China and Inner Mongolia, whereas Cr and Pb for Tibet and Yunnan, respectively. Compared to health risk assessment, improved models increased risk assessment accuracy and provided recommended exposure parameter for high-risk populations. This study will provide new insights for soil-related health risk assessment.

Source apportionment and human health risk of PAHs accumulated in edible marine organisms: A perspective of "source-organism-human".

Most PAHs produced by human activities can be absorbed and accumulated by edible organisms and pose a potential hazard to human health. However, the source apportionment and human health risk of PAHs accumulated in edible organisms remains largely unknown. Therefore, we conducted source analysis and health risk assessment based on the PAH concentrations in ten marine fish from coastal areas of Guangdong, China. Results showed that the pollution of PAHs in fish organisms was at "Minimally polluted" level, and that all marine fish had the ability to accumulate PAHs. Risk assessment indicated Carcinogenic risk of PAHs in four populations was at a "Cautionary risk" level, with urban children suffered the highest risk. Petroleum pollution, Coal and biomass combustion, and Marine transport emissions were identified as the main anthropogenic sources for PAHs in organisms, and Marine transport emissions accounted for the highest Carcinogenic risk. The Acceptable daily intake for all populations were far below their actual daily intake without causing "Cautionary risk". Our findings provide new insights into the source apportionment and health risk of PAHs from a "source-organism-human" perspective, and suggested that joint management of three anthropogenic sources would be an effective way to prevent the health risks of PAHs.

Determination of priority control factors for the management of soil trace metal(loid)s based on source-oriented health risk assessment.

Trace metal(loid)s (TMs) in soils can seriously threaten the ecological environment and human health. With the limitation of resources and costs, determining priority control factor is critical for managing soil TM pollution. To explore the pollution characteristics, source apportionment, and human health risk of TMs, a total of 209 surface soil samples were collected from Anqing City, China. Results showed that all the average values of TM concentration, except for Cr, were higher than their corresponding background value. Using a Positive matrix factorization model coupled with Correlation analysis, four sources (including agricultural sources, atmospheric deposition sources, industrial sources, and natural sources) were identified as the determinants for the accumulation of soil TMs, with the contribution rates of 12.4%, 8.1%, 64.1%, and 15.4%, respectively. The assessment of probabilistic health risks revealed that Non- carcinogenic risks of all populations were acceptable (HI < 1), while Carcinogenic risks were all at a high level (TCR > 10E-04). Agricultural pollution and As were identified as priority control factors, according to the analysis results of the relationship among TMs, pollution sources and health risks. Our findings provide scientific support for decision-makers to formulate target control policies and reduce management costs of soil pollution.

Do trace metal(loid)s in road soils pose health risks to tourists? A case of a highly-visited national park in China.

Nowadays, more people tend to spend their recreational time in large national parks, and trace metal(loid)s in soils have attracted long-term attention due to their possible harm to human health. To investigate the pollution levels, potential sources and health risks of trace metal(loid)s in road soils, a total of eight trace metal(loid)s (including As, Cd, Cr, Cu, Ni, Pb, Zn and Hg) from 47 soil samples along roads were studied in the Huangshan National Park in Southeast China. The results showed that the concentrations of As, Cd, Pb, Zn and Hg appeared different degrees of pollution compared with their corresponding background values. According to the pollution indices, Hg and Cd were recognized as significant pollutants presenting moderate to high ecological risk. Combining principal component analysis and positive matrix factorization model, the results showed that traffic, industrial, agricultural and natural sources were the potential origins of trace metal(loid)s in this area, with contribution rates of 39.93%, 25.92%, 10.53% and 23.62%, respectively. Non-carcinogenic risks were all negligible, while the carcinogenic risk of As was higher than the limit (1 × 10-6). Moreover, children were more susceptible to trace metal(loid)s by ingestion which appeared to be a more important exposure pathway than dermal contact and inhalation. The contribution rates of different sources to non-carcinogenic risks and carcinogenic risks were similar among children and adults, while traffic and industrial sources have a significant impact on health risks. This study will give more insights to control the environmental risks of trace metal(loid)s in national parks.

Anti-oxidant mechanisms of Chlorella pyrenoidosa under acute GenX exposure.

GenX, a substitute for perfluorooctanoic acid, has been widely detected in surface water. Due to its bioaccumulation, toxicity and persistence, GenX can cause adverse effects such as oxidative damage on aquatic organisms. To investigate the toxicity of GenX and the anti-oxidant mechanism of algae under acute exposure, the growth, photosynthetic activity and gene expression of Chlorella pyrenoidosa (C. pyrenoidosa) were tested. Results showed that the growth of C. pyrenoidosa was inhibited under acute GenX exposure. The toxicity of GenX increased with time and concentration but was lower than that of the traditional perfluoroalkyl and polyfluoroalkyl substances (PFASs). Furthermore, with the increase of GenX concentration, the production of reactive oxygen species increased, while the level of the anti-oxidant enzyme first increased and then decreased. Changes in photosynthetic parameters also indicated that the photosynthetic system of C. pyrenoidosa was negatively affected by GenX exposure. Transcription analysis revealed that the up-regulation of genes related to the glutathione-ascorbate cycle and photosynthesis is a positive strategy to cope with the oxidative stress caused by acute GenX exposure. Our findings provide new insights into the interactions between emerging PFASs and aquatic organisms at the molecular level.

Health risk assessment of heavy metal(loid)s in park soils of the largest megacity in China by using Monte Carlo simulation coupled with Positive matrix factorization model.

Urban Parks are important places for residents to engage in outdoor activities, and whether heavy metal(loid)s (HMs) in park soils are harmful to human health has aroused people's concern. A total of 204 topsoil samples containing nine HMs were collected from 78 urban parks of Shanghai in China, and used to assess the health risks caused by HMs in soils. The results revealed that the Hg, Cd and Pb were the main enriched pollutants and posed higher ecological risks than the other HMs. Four HM sources (including natural sources, agricultural activities, industrial production and traffic emissions) were identified by combining the Positive matrix factorization model and Correlation analysis, with the contribution rate of 48.24%, 7.03%, 13.04% and 31.69%, respectively. The assessment of Probabilistic health risks indicated that the Non-carcinogenic risks for all populations were negligible. However, the Total carcinogenic risk cannot be negligible and children were more susceptible than adults. The assessment results of source-oriented health risks showed that industrial production and traffic emissions were estimated to be the most important anthropogenic sources of health risks for all populations. Our results provide scientific support needed for the prevention and control of HM pollution in urban parks.

Risk Assessment and Source Apportionment of Soil Heavy Metals under Different Land Use in a Typical Estuary Alluvial Island.

Understanding the environmental risks of soil heavy metals (HMs) and identifying their main sources are the essential prerequisites for the prevention and management of soil pollution. Based on a detailed survey of soil HMs (Cu, Cr, Ni, Zn, Pb, Cd, As and Hg) from different land use types (including agricultural land, construction land, wetland, and forest land) in an estuary alluvial island, the environmental risk and source apportionment of soil HMs were investigated. Altogether, 117 soil samples were taken in the study area to appraise the soil HMs environmental risk by using the geo-accumulation index (Igeo), potential ecological risk index (RI), and human health risk assessment (HRA) and to identify its main sources by using positive matrix factorization (PMF) model. The average concentrations of soil HMs (except As) surpassed their reference background values in China. There were no significant differenced in the mean concentrations of HMs in different land use types, except that the Hg concentration in the construction land was significantly higher than that in others. The results of Igeo showed that Cd pollution was unpolluted to moderately polluted, and that the others were unpolluted. The potential ecological risk level for Cd and Hg was "moderated potential risk", while for Cu, Cr, Ni, Zn, Pb and As was "low potential risk". Higher contamination was distributed at the west-central area. The results of the HRA indicated that the non-carcinogenic risk and the carcinogenic risk that human beings suffered from HMs in different land uses were insignificant. To more accurately identify the sources of soil HMs, the PMF model coupled with the GIS-spatial analysis was applied. The results showed that agricultural activities, natural source, industrial discharge and river transportation, and atmosphere deposition were the main determining factors for the accumulation of soil HMs in the study area, with the contribution rate of 24.25%, 23.79%, 23.84%, and 28.12%, respectively. The study provides an underlying insight needed to control of the soil HM pollutions for an estuary alluvial island.

Bioaccumulation and health risk assessment of heavy metals to bivalve species in Daya Bay (South China Sea): Consumption advisory.

Bivalves are one of the key components of the biogeochemical cycle in the marine system, and respond to heavy metal (HM) sensitively as filter feeders. To determine relationship of HMs in edible bivalve and seawater and HM effects on human health when digesting bivalves, HMs were analyzed in bivalves and seawater. The results showed that the mean HM concentrations in bivalves decreased in the order of Zn > Cu > Cr > Pb > As > Cd > Hg. Generally, all the bioconcentration factor values of bivalves were higher than 100, suggesting that bivalves have a high bioaccumulation ability. Nonmetric multidimensional scaling analysis indicated that all bivalves have a high bioaccumulation capacity for Cu and Zn. It was found that there are health risks associated with consuming bivalves, and children are more vulnerable than adults. Finally, the maximum allowable consumption rates of non-carcinogenic and carcinogenic risk were determined. These results provide the underlying insights needed to guide the consumption of seafood.

Transcriptional and Physiological Responses to Nutrient Loading on Toxin Formation and Photosynthesis in Microcystis Aeruginosa FACHB-905.

An important goal of understanding harmful algae blooms is to determine how environmental factors affect the growth and toxin formation of toxin-producing species. In this study, we investigated the transcriptional responses of toxin formation gene (mcyB) and key photosynthesis genes (psaB, psbD and rbcL) of Microcystis aeruginosa FACHB-905 in different nutrient loading conditions using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Three physio-biochemical parameters (malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH)) were also evaluated to provide insight into the physiological responses of Microcystis cells. We observed an upregulation of mcyB gene in nutrient-deficient conditions, especially in nitrogen (N) limitation condition, and the transcript abundance declined after the nutrient were resupplied. Differently, high transcription levels were seen in phosphorus (P) deficient treatments for key photosynthesis genes throughout the culture period, while those in N-deficient cells varied with time, suggesting an adaptive regulation of Microsystis cells to nutrient stress. Increased contents of antioxidant enzymes (SOD and GSH) were seen in both N and P-deficient conditions, suggesting the presence of excess amount of free radical generation caused by nutrient stress. The amount of SOD and GSH continued to increase even after the nutrient was reintroduced and a strong correlation was seen between the MDA and enzyme activities, indicating the robust effort of rebalancing the redox system in Microcystis cells. Based on these transcriptional and physiological responses of M. aeruginosa to nutrient loading, these results could provide more insight into Microcystis blooms management and toxin formation regulation.

Mechanism and Reaction Pathways for Microcystin-LR Degradation through UV/H2O2 Treatment.

Microcystin-LR (MCLR) is the most common cyanotoxin in contaminated aquatic systems. MCLR inhibits protein phosphatases 1 and 2A, leading to liver damage and tumor formation. MCLR is relatively stable owing to its cyclic structures. The combined UV/H2O2 technology can degrade MCLR efficiently. The second-order rate constant of the reaction between MCLR and hydroxyl radical (·OH) is 2.79(±0.23)×1010 M-1 s-1 based on the competition kinetics model using nitrobenzene as reference compound. The probable degradation pathway was analyzed through liquid chromatography mass spectrometry. Results suggested that the major destruction pathways of MCLR were initiated by ·OH attack on the benzene ring and diene of the Adda side chain. The corresponding aldehyde or ketone peptide residues were formed through further oxidation. Another minor destruction pathway involved ·OH attack on the methoxy group of the Adda side chain, followed by complete removal of the methoxy group. The combined UV/H2O2 system is a promising technology for MCLR removal in contaminated aquatic systems.