Physical quality of seeds of Moquiniastrum polymorphum / Qualidade física de sementes de Moquiniastrum polymorphum

Abstract This study aimed to analyze the physical properties of the seeds of Moquiniastrum polymorphum when processed by mini-SAS, X-ray and seed blower systems. The seeds were purchased from Seeds Caiçara, located in Penápolis-SP. After purchase, the seed lot was characterized by standard tests. In the X-ray test the seeds were classified according to their internal morphology as filled, unfilled or malformed. The seed blower was set at six opening settings, resulting in light and heavy fractions. The evaluations were performed by weighing the seeds that remained in the blower (heavy fraction) and by the amount of filled seeds indicated by the X-ray test. The germination tests were composed of four replicates of 50 seeds. The mini-SAS was used for external evaluation. It was concluded that the combined use of the seed blower and the X-ray test was efficient for the evaluation and determination of the physical quality of the seeds of M. polymorphum. The seed blower at opening settings 5 resulted in the best lot quality. The external parameters were not able to qualitatively evaluate the seeds of M. polymorphum.

Resumo O presente estudo objetivou analisar as propriedades físicas das sementes de Moquiniastrum polymorphum por meio dos equipamentos raios-X, mini-SAS e soprador de sementes. As sementes foram adquiridas da empresa Sementes Caiçara em matrizes localizadas no município de Penápolis-SP. Após adquirido e beneficiado, o lote de sementes foi caracterizado por meio de testes padrões. No teste de raios-X as sementes foram classificadas de acordo com sua morfologia interna como cheias, vazias ou mal formadas. O soprador de sementes foi ajustado em seis aberturas, resultando em frações leves e pesadas. As avaliações foram realizadas pesando-se as sementes que permaneceram no soprador (fração pesada) e pela quantidade de sementes cheias pela análise de raios-X. Posteriormente foram realizados testes de germinação compostos por quatro repetições de 50 sementes. Na avaliação externa das sementes utilizou-se o equipamento mini-SAS. Após análise dos dados, concluiu-se que a utilização em conjunto dos equipamentos soprador de sementes e raios-X foi eficiente para avaliação e determinação da qualidade física das sementes de M. polymorphum. O soprador de sementes na regulagem da abertura 5 resultou na melhor qualidade do lote. Os parâmetros externos não foram capazes de avaliar qualitativamente as sementes de M. polymorphum.

CA15.3, CEA and LDH in dogs with malignant mammary tumors.

BACKGROUND: Presence of tumor markers in serum might be connected to the number of secreting cells and with the stage of the neoplasm. However, there are few studies regarding these markers in veterinary clinical oncology. OBJECTIVES: To determine the serum concentrations of cancer antigen 15.3 (CA 15.3), carcinoembryonic antigen (CEA), and lactate dehydrogenase (LDH) in female dogs with different stages of mammary cancer. ANIMALS: Ninety female dogs, including 30 that were healthy, 40 that had nonmetastatic cancer, 12 with regional metastasis, and 8 with distant lymph node metastasis. METHODS: Prospective case-controlled observational study. Serum samples were collected to measure CA15.3, CEA, and LDH from 60 female dogs with mammary cancer during mastectomy and 30 healthy female dogs during routine check-up. CA15.3 and CEA were determined by chemiluminescent immunoassay and LDH by ultraviolet kinetic method. Western blotting analysis was performed to confirm the specificity and possible cross-reactivity of human CA15.3 and CEA antibodies with canine serum. Group data were compared by ANOVA followed by Student-Newman-Keuls and Tukey's tests. Correlations were investigated by Pearson and Spearman tests. RESULTS: CEA, CA15.3, and LDH were measurable in all groups. Higher serum concentration of CA15.3 and LDH was associated with regional and distant metastases. There was a significantly higher serum CA15.3 concentration in animals with lymph node metastasis when compared with animals without metastasis. There were no significant differences in CEA among groups. Expression of CA15.3 and CEA in canine serum was confirmed by Western blotting. CONCLUSIONS AND CLINICAL IMPORTANCE: Serum CA15.3 can be used to distinguish nonmetastatic from metastatic carcinomas.

Reconstruction after extended orbital exenteration using a fronto-lateral flap.

Orbital exanteration is usually performed for advanced neoplasms of the eyelids and is associated with significant distress for patients. Its reconstruction should aim for functional and aesthetic results and safety for further oncological treatment. The ideal reconstruction should provide adequate cutaneous covering, short healing time, obliteration and closure of communication with facial sinuses and nasal cavity, resistance to radiotherapy, low morbidity and favorable rehabilitation. In 10 patients, a flap based on the frontal branch of the temporal artery was used for immediate reconstruction after orbital exenteration. Flap demarcation began through the frontal midline from the glabella to 0.5 cm above the hairline. Its elevation was performed in a plane above the periosteum and galea, including skin, subcutaneous tissue and frontal muscle. It was rotated tension-free over the defect. In all patients, adequate closure of the defect and obliteration of the orbital cavity was achieved. There was no flap loss or major complications. The lateral frontal flap is easy and fast to execute, with a reliable blood supply and reproducible technique. Its main applicability is for patients with orbital defects and high surgical risks with contraindications for microsurgical reconstruction.

Transferencia da posicao sentada pra em pe: estudo comparativo entre idosas assintomaticas e idosas com osteoartite e correlacao entre tempo da tarefa e funcao muscular / Sit-to-stand transfer: comparasion between asymptomatic and osteoarthritic elderly women and correlation between movement time and muscle function

Em idosos, a capacidade de se levantar de uma posicao sentada sentada em um menor tempo associa-se a menos comorbidades ec para realizar essa atividade. Forca e potencia de extensores e plexores de joelho sao importantes. ao aumentar a velocidade do levantar

Transgene elimination in genetically modified dry bean and soybean lines

Transgene elimination is a poorly studied phenomenon in plants. We made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. In both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. Due to its potential biotechnological value, this transgenic line was micropropagated by grafting and the vegetative copies were studied for more than two years. More than 300 plants of progeny were obtained during this period, demonstrating that the phenomenon of elimination was consistently repeated and offering an opportunity for detailed study of transgene elimination, including the characterization of the integration sites. Cloning and sequencing of the transgenic loci, reciprocal crosses to untransformed plants, genomic DNA blots, and GUS assays were performed in the transgenic lines. Based on the molecular and genetic characterization, possible mechanisms involved in transgene elimination include intrachromosomal recombination, genetic instability resulting from the tissue culture manipulations, and co-elimination of transgenes, triggered by a process of genome defense.

Relacao isquiotibiais/quadriceps em mulheres idosas utilizando o dinamometro isocinetico / Isokinetic hamstring/quadriceps ratio in elderly women

A relacao entre o torque isocinetico maximo dos isquiotibiais e o torque isocinetico maximo do quadriceps (relacao IQT/QUA) e um parametro utilizado para descrever o equilibrio muscular da articulacao do joelho. Uma alteracao desse equilibrio predispoe a articulacao ou o grupo muscular mais fraco a lesao. A obtencao de valores de referencia da relacao IQT/QUA de idosas e importante, p[ois possibilita comparacoes entre individuos dessa faixa etaria. O objetivo deste estudo foi estabelecer valores normativos para a relacao IQT/QUA de idosas nao-institucionalizadas que vivem na regiao metropolitana de Belo Horizonte. Este foi um estudo transversal de uma amostra composta por 47 idosas voluntarias (69,11+-3,64 anos). Como nao houve diferenca estatisticamente significativa entre os membros, foram descritos apenas a media, o desvio padrao, o coeficiente de variacao (CV) e o erro estimado (EE) da relacao IQT/QUA do membro inferior dominante, nas velocidades angulares de (60s e 180s), utilizando o dinamometro isocinetico Biodex System 3 Pro. Na velocidade de 60s, a media foi de 47,95+-10,99(por cento); o Cv, 22,91; e o EE, 6,85. Na velocidade de 180s a media foi 59,59+-13,40(por cento); o Cv, 22,49; e o EE, 6,43. Assim, o valor da relacao foi maior com o aumento da velocidade. Os resultados aqui encontrados, aliados a outros de diferentes regioes, podem ser considerados referencia para idosas vivendo na comunidade, o que podera direcionar a reabilitacao, tornando-a mais efetiva. Alem disso, esses dados permitirao comparacoes com futuros estudos que envolvam populacoes de idosas comunitarias, institucionalizadas ou com alguma doenca

Estudo anatômico e morfométrico do retalho peritoneal da artéria epigástrica inferior profunda / Anatomic and morphometric study of the peritonal branch of the deep inferior epigastric artery

As reconstruções de defeitos de mucosa, nas regiões da cabeça e pescoço e região vaginal, assim como as lesões em mão e membros inferiores secun- dárias à ressecção de tumores, traumas e infecções, entre outros, constituem um desafio para o cirurgião plástico. Em 1990, Mixter descreveu a utilização do retalho composto fascioperitoneal, baseado no ramo perfurante peritoneal da artéria epigástrica inferior profunda (AEIP). Este retalho pode ser dissecado junto com o músculo reto abdominal e/ou associado a uma ilha de pele. O peritônio tem grande capacidade de metaplasia escamosa e é resistente à exposição à secreções gástricas e radiação. Foi realizado um estudo anatômico em 17 cadáveres, totalizando 34 regiões anatômicas, com os objetivos de determinar os parâmetros de dissecção deste retalho e o território vascular anatômico do ramo peritoneal da AEIP. Assim mesmo, foi realizado o estudo morfométrico do vaso. O retalho peritoneal da AEIP possui um pedículo vascular constante, de localização anatômica relativamente fixa, com um bom arco de rotação, e pode ser dissecado em dimensões que permitem a cobertura de defeitos de mediano e grande porte.

The reconstruction of mucosal defects in the regions of head and neck, and vaginal region, and secondary hand and lower limb defects after tumor resection, trauma and infections, among others, represent a challenge for the plastic surgeon. In 1990, Mixter described the use of the composed fascioperitoneal flap, based in the peritoneal branch of the deep inferior epigastric artery (DIEA). This flap can be dissected together with the rectus abdominis muscle and/or associate to a skin island. Peritoneum has great capacity of squamous metaplasia and is resistant to gastric secretions and radiation exposure. An anatomical study in 17 cadavers was carried through, totalizing 34 anatomical regions, with the objective to determine the dissection parameters of this flap and the anatomical vascular territory of the DIEA peritoneal branch. A morphometric study of the vessels was also done. The peritoneal branch of the DIEA has one constant vascular pedicle, with relatively permanent anatomical localization, with a good arc of rotation, and can be dissected in dimensions that allow coverage of medium and great size defects.

[Pulmonary lesion in electric injury: report of a case]. / Comprometimento pulmonar em trauma elétrico: relato de caso.

Major electrical injuries constitute approximately 5% of all admissions to Burn Units. Visceral complications are associated with a high mortality rate. The most common visceral lesions associated to electric burns are cardiac lesions. Pulmonary compromise is rare, if compared to inhalation injuries in termical burns. Although, when the entry or exit ports are the toracic wall, pleural effusion, hemotorax and pneumonitis may occur. A rare case of high-voltage electrical injury with massive pulmonary lesion is presented, regarding to clinical course and roentgenographic patterns.

[Microsurgical reimplantation of scalp: case report]. / Reimplante microcirúrgico de couro cabeludo: relato de caso.

With the introduction of modern techniques in plastic surgery, severe injuries concerning the extremities and scalp are now amenable to surgical treatment. Successful reimplantation of the scalp either total or partial have been rarely reported in the literature. We treated a patient with avulsion affecting 2/3 of the scalp, the right ear and a cutaneous flap on right side of the face. We report here the initial care and operative procedure. The avulsed scalp was revascularized through microanastomoses of the temporal artery and vein. The reimplantation was well succeed and cosmetic results were satisfactory.

[The early correction of the hand deformities in Apert's syndrome. A preliminary report]. / Correção precoce das deformidades das mãos na sindrome de Apert. Communicação preliminar.

The correction of cranial and limb malformations of the Apert's syndrome requires efforts of a multidisciplinary team. The esthetic and functional repercussions that this syndrome brings to their carriers are severe and so the treatment should aim tom improve the function of the hands in order to integrate these patients in a more normal social live. In our Service, in the period from 1989 to 1992 five patients carrying this syndrome had their hand correction completed before the age of three years. It was observed that surgery was possible in this early stage with no additional complication rate. Quadrangular flaps for the neocomissure and for the coverage of the fingers were successfully used even with skeletal deviations. There was improvement of the esthetics of the hands, but their function could not be evaluated properly. Anyway, because of complex abnormalities the results will never be perfect. The early treatment of these alterations allows the gain of a better hand function and moreover accelerates the physical and intellectual development of the children.

Tratamento dos angiomas do labio / Treatment of lip angiomas

Foram estudados 25 pacientes portadores de angiomas de labio, sendo 17 maduros (15 venosos, 2 complexos) e oito imaturos (7 tuberosos, 1 strawberry). Existem varios tipos de tratamento propostos pela literatura. Dentre os varios metodos, os autores, apos dez anos de experiencia, optaram preferencialmente por cirurgia, cirurgia mais embolizacao e corticoide sistemico. O uso de corticoide local e de agentes esclerosantes foram utilizados apenas como coadjuvantes da cirurgia. A cirurgia, realizada em uma ou mais etapas, se mostrou alternativa segura e eficiente para angiomas maduros, com excecao dos complexos, imaturos com complicacoes e imaturos que nao incluiram ate os cinco anos de idade.

Tratamento cirurgico da hidradenite supurativa / Surgical treatment of hidradenitis suppurativa

A hidradenite supurativa e uma doenca cutanea de evolucao cronica e localizacao peculiar, intimamente relacionada a presenca de glandulas sudoriparas do tipo apocrino. Resulta de obstrucao dos foliculos glandulares com subsequente rotura e infeccao local. Apresenta progressiva evolucao se nao tratada adequadamente, incorrendo em acentuada morbidade. Devido a escassez de relatos semelhantes, o tratamento da hidradenite permanece assunto controverso, nao havendo ate o momento, normatizacao das condutas a serem empregadas em tais casos. O presente estudo visou a analise da eficacia das medidas terapeuticas empregadas em 32 casos de pacientes que necessitaram de internacao no HCFMUSP, no periodo de 1985 a 1990. Com relacao a localizacao, 75 por cento dos pacientes possuiam lesoes perianais, seguida por incidencia similar de lesoes axilares e inguinoperineais em cerca de 40 por cento dos casos. Utilizando-se os indices de recidiva e evolucao morbida da doenca, pode-se verificar a eficiencia dos metodos terapeuticos. Tendo em vista a necessidade de intervencao com resultados mais imediatos e eficazes, o tratamento cirurgico constitui-se na melhor opcao.

[Treatment of lip angiomas]. / Tratamento dos angiomas do lábio.

The experience with the treatment of 25 patients with angiomas of the lips is presented. Among the several methods of treatment indicated in the literature, the authors have experimented with surgery, surgery and embolization, and systemic corticotherapy. Local application of corticosteroids and sclerosing agents were used just as a complementary therapy to the surgery. Surgery performed in one or more stages when necessary, were considered a safe and reliable method for treatment of mature venous angiomas, as well as for immature ones with no involution after a period of five years.