Ecotoxicology of Heavy Metal(loid)-Enriched Particulate Matter: Foliar Accumulation by Plants and Health Impacts.

Atmospheric contamination by heavy metal-enriched particulate matter (metal-PM) is highly topical nowadays because of its high persistence and toxic nature. Metal-PMs are emitted to the atmosphere by various natural and anthropogenic activities, the latter being the major source. After being released into the atmosphere, metal-PM can travel over a long distance and can deposit on the buildings, water, soil, and plant canopy. In this way, these metal-PMs can contaminate different parts of the ecosystem. In addition, metal-PMs can be directly inhaled by humans and induce several health effects. Therefore, it is of great importance to understand the fate and behavior of these metal-PMs in the environment. In this review, we highlighted the atmospheric contamination by metal-PMs, possible sources, speciation, transport over a long distance, and deposition on soil, plants, and buildings. This review also describes the foliar deposition and uptake of metal-PMs by plants. Moreover, the inhalation of these metal-PMs by humans and the associated health risks have been critically discussed. Finally, the article proposed some key management strategies and future perspectives along with the summary of the entire review. The abovementioned facts about the biogeochemical behavior of metal-PMs in the ecosystem have been supported with well-summarized tables (total 14) and figures (4), which make this review article highly informative and useful for researchers, scientists, students, policymakers, and the organizations involved in development and management. It is proposed that management strategies should be developed and adapted to cope with atmospheric release and contamination of metal-PM.

Hydrogeochemical investigation of arsenic in drinking water of schools and age dependent risk assessment in Vehari District, Punjab Pakistan: a multivariate analysis.

Globally, a large number of school-aged children is suffering from water-borne diseases, particularly in low-income countries. Arsenic (As) is a hazardous and potentially carcinogenic metal(loid) in drinking water. Nowadays, alarming levels of As have been reported in the groundwater of Vehari District, Punjab Pakistan. In this study, drinking water supplies for high and higher secondary schools were examined in Vehari District. A total of 164 water samples were collected from schools and subjected to heavy metal(loid) analysis (As) and basic water physicochemical parameters. The results were analyzed with respect to sampling area, school type, school education level, sources of sample collection, and the depth of the source. The results revealed that As concentration of water samples in boys' and girls' schools was 12.8 µg/L and 9.2 µg/L, respectively. However, when the As concentration in drinking water was evaluated at the school education level, a notable higher concentration of As was observed in the higher secondary schools than the high schools with an average of 19.5 and 9.7 µg/L, respectively. The risk assessment indices were calculated based on education level and different age groups of the children (primary, elementary, high, and higher secondary). High carcinogenic (cancer risk = 0.001) and non-carcinogenic (hazard quotient = 2.0) risks were noted for the children in higher secondary school. The current findings anticipated that the drinking water of schools in Vehari District did not meet the requirement of the World Health Organization (WHO) drinking water quality guidelines. Safe drinking water is crucial for the development and growth of children. Therefore, it is important for educational authorities to take steps for provision of As free safe drinking water to students and local inhabitants.

Risk assessment and biophysiochemical responses of spinach to foliar application of lead oxide nanoparticles: A multivariate analysis.

Despite extensive research progress in the recent past, the data regarding foliar uptake of heavy metals, associated biophysiochemical changes inside plants and possible health hazards are limited. This study determined the effect of foliar application of lead oxide nanoparticles (PbO-NPs) on lead (Pb) accumulation, physiological and biochemical changes inside spinach plants and associated health risks. A green method was used to prepare PbO-NPs using coconut water. Scanning electron microscopy (SEM) showed the preparation of smooth, unwrinkled, granular and spherical PbO-NPs. Spinach leaves were exposed via foliar application to three concentrations of PbO-NPs (0, 10 and 50 mg/plant). Foliar PbO-NPs application resulted in a significant accumulation of Pb in leaves (42.25 µg g-1), with limited translocation towards root tissues (4.46 µg g-1). This revealed that spinach can accumulate considerable amount of Pb via foliar uptake. Lead accumulation inside spinach caused a significant decrease in pigment contents (38%) and dry weight (67%). After foliar uptake, Pb caused several-fold increase in the activities of catalase and peroxidase. However, foliar PbO-NPs did not induce significant changes in H2O2 production, lipid peroxidation and superoxide dismutase activity. Application of PbO-NPs (50 mg/plant) showed possible health risks (non-carcinogenic) due to ingesting Pb-contaminated leaves of spinach. It is proposed that atmospheric contamination and foliar deposition of metal-PM can seriously affect vegetable growth and can provoke health issues due to ingestion of metal-enriched vegetables. Therefore, atmospheric levels of heavy metals need to be monitored on a regular basis to avoid their food chain contamination and possible human exposure.

A multivariate analysis of health risk assessment, phytoremediation potential, and biochemical attributes of Spinacia oleracea exposed to cadmium in the presence of organic amendments under hydroponic conditions.

Cadmium (Cd) phytoremediation potential and its accumulation in edible and nonedible plant tissues is the function of various biochemical processes taking place inside plants. This study assessed the impact of organic ligands on Cd phyto uptake and different biophysiochemical processes of Spinacia oleracea L., and associated health hazards. Plants were exposed to Cd alone and chelated with citric acid (CA) and ethylenediaminetetraacetic acid (EDTA). Results revealed that the effect of Cd on lipid peroxidation, H2O2 production and pigment contents varied greatly with its applied level and the type of organic ligand. Moreover, the effect was more prominent in root tissues than leaf tissues and for high concentrations of Cd and organic ligands. Cadmium accumulation increased by 90 and 74% in roots and leaves, respectively, with increasing Cd levels (25-100 µM). Cadmium exposure at high levels caused lipid peroxidation in roots only. Application of both CA and EDTA slightly diminished Cd toxicity with respect to pigment contents, lipid peroxidation and hydrogen peroxide (H2O2) contents. Hazard quotient (HQ) of Cd was <1.00 for all the treatments. Under nonlinear effect of treatments, multivariate analysis can be an effective tool to trace overall effects/trends.

Effect of organic amendments on cadmium stress to pea: A multivariate comparison of germinating vs young seedlings and younger vs older leaves.

Despite significant recent advancement in research, biogeochemical behavior of heavy metals with respect to their applied form is still topical. Moreover, metal toxicity to plants may vary with their stage of development/maturity. Therefore, this study for the first time evaluated the influence of ethylenediaminetetraacetic acid (EDTA) and citric acid (CA) on cadmium (Cd) accumulation and toxicity to germinating and young pea seedlings as well as in younger and older leaves. The experimental setup of current study consisted of two separate studies. The first study was performed on germinating seedlings grown in a Cd-contaminated sand media. Pea seeds were treated with two levels of Cd (Cd-25 and Cd-100) alone and combined with different levels of EDTA and CA. The second study was carried out in hydroponic solution. The influence of organic amendments on Cd accumulation and toxicity to pea plants was evaluated by determining Cd contents in pea seedlings, H2O2 contents, chlorophyll contents and lipid peroxidation in younger and older leaves. Cadmium stress caused overproduction of H2O2 in roots and leaves of pea seedlings. Cadmium-induced overproduction of H2O2 caused a decrease in the pigment contents and increased lipid peroxidation. Application of EDTA at higher levels (81 and 200µM) increased Cd accumulation by pea plants. However, CA did not affect Cd accumulation by pea. Both EDTA and CA increased Cd-induced H2O2 production and lipid peroxidation. Younger pea leaves showed more sensitivity to Cd stress compared to older leaves. Similarly, Cd toxicity was more pronounced in germinating seedlings than young seedlings. Moreover, Pearson correlation and principal component analysis (PCA) showed very interesting correlations between treatments and stress responses of germination and young seedlings as well as younger and older leaves. Based on multivariate analysis, it is proposed that the Cd toxicity to pea plants greatly vary with its growth stage and the maturity of organs (younger or older leaves).