RESUMO
The results of studying the effect of the anthocyanin-containing complex from Sorbus aucuparia L. on the main indicators of erythropoiesis in the blood and bone marrow of mice with Lewis lung carcinoma against the background of doxorubicin administration were presented. It was shown that administration of the anthocyanin-containing complex from S. aucuparia L. to animals with the tumor prevented the development of anemic syndrome by promoting regeneration of the erythropoiesis after its depletion caused by single administration of a cytostatic agent.
Assuntos
Carcinoma Pulmonar de Lewis , Citostáticos , Sorbus , Animais , Antocianinas/farmacologia , Carcinoma Pulmonar de Lewis/tratamento farmacológico , Eritropoese , CamundongosRESUMO
Paclitaxel in a single MTD of 40 mg/kg caused chromosome aberrations and genome changes (polyploidy) in the bone marrow cells of mice early and 3 months after the injection. The quantity of early precursors of erythropoiesis in the bone marrow decreased, as did their proliferative potential irrespective of the animal gender. Injection of paclitaxel in the MTD caused the development of bone marrow hypoplasia during the early period of observation (up to 14 days) and 3 months after injection.
Assuntos
Células da Medula Óssea/efeitos dos fármacos , Genoma/efeitos dos fármacos , Hematopoese/efeitos dos fármacos , Paclitaxel/farmacologia , Animais , Antineoplásicos/farmacologia , Células da Medula Óssea/metabolismo , Aberrações Cromossômicas/induzido quimicamente , Aberrações Cromossômicas/efeitos dos fármacos , Análise Citogenética , Eritropoese/efeitos dos fármacos , Eritropoese/genética , Feminino , Instabilidade Genômica/efeitos dos fármacos , Hematopoese/genética , Masculino , Camundongos , Camundongos Endogâmicos CBA , Testes de MutagenicidadeRESUMO
The experiments on C57Bl/6 mice with Lewis lung carcinoma showed that addition of Tussilago farfara L. polysaccharides to conventional cisplatin/paclitaxel polychemotherapy moderated neutropenia caused by antitumor therapy and increased its efficiency. The stimulating effect of polysaccharides on the granulopoietic lineage cells is comparable with that of recombinant CSF Neupogen.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Carcinoma Pulmonar de Lewis/tratamento farmacológico , Cisplatino/farmacologia , Paclitaxel/farmacologia , Polissacarídeos/farmacologia , Tussilago/química , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Carcinoma Pulmonar de Lewis/imunologia , Carcinoma Pulmonar de Lewis/patologia , Feminino , Filgrastim/farmacologia , Granulócitos/efeitos dos fármacos , Granulócitos/imunologia , Granulócitos/patologia , Fármacos Hematológicos/farmacologia , Contagem de Leucócitos , Camundongos , Camundongos Endogâmicos C57BL , Extratos Vegetais/química , Polissacarídeos/isolamento & purificação , Resultado do Tratamento , Carga Tumoral/efeitos dos fármacosRESUMO
We studied toxicity of a new Russian radiopharmaceutical Nanocolloid, (99m)Tc-Al2O3. Tests for acute toxicity showed that this agent belongs to a class of moderate-toxicity substances and does not have cumulative properties. The evaluation of subchronic toxicity after subcutaneous injection of this product to rats (0.04, 0.2, and 0.4 ml/kg) and rabbits (0.02 and 0.2 ml/kg) for 7 days did not reveal changes in the general state, temperature, body weight, indices of the peripheral blood and bone marrow, functions of the heart, liver, kidneys, and nervous system, and morphological characteristics of the internal organs in animals. The drug does not produce a local irritant effect.
Assuntos
Compostos Radiofarmacêuticos/efeitos adversos , Óxido de Alumínio/efeitos adversos , Animais , Temperatura Corporal/efeitos dos fármacos , Feminino , Coração/efeitos dos fármacos , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Masculino , Camundongos , Nanopartículas/efeitos adversos , Sistema Nervoso/efeitos dos fármacos , Coelhos , Ratos , Tecnécio/efeitos adversosRESUMO
We studied the dynamics of erythropoiesis in CBA mice during gestation against the background of treatment with iron-binding drug. The mechanisms of suppression of the bone marrow erythroid stem were evaluated. Administration of deferoxamine in a dose of 1 g/kg induced hypoplasia of the erythroid hemopoietic lineage. Suppression of bone marrow erythropoiesis manifested in a decrease of hemoglobin concentration and counts of reticulocytes, erythrocytes, and erythrokaryocytes. These changes were accompanied by a decrease in functional activity of erythropoietic precursors and secretion of erythropoietically active humoral factors by bone marrow myelokaryocytes. These data indicate that deferoxamine can be used for modeling of iron defi ciency anemia in pregnancy.
Assuntos
Anemia Ferropriva/induzido quimicamente , Medula Óssea/efeitos dos fármacos , Desferroxamina/farmacologia , Eritropoese/efeitos dos fármacos , Eritropoese/fisiologia , Sideróforos/farmacologia , Animais , Contagem de Células Sanguíneas , Medula Óssea/metabolismo , Eritrócitos/citologia , Feminino , Camundongos , Camundongos Endogâmicos CBA , Gravidez , Reticulócitos/citologiaRESUMO
Beginning with the first hours of experiments, cisplatin evoked an increase of chromosomal aberrations in CBA/CaLac bone marrow cells. Significant increase of structural infringements of chromosomes due to chromatid breaks was revealed in metaphase plates of murine bone marrow preparations through 24 h after cisplatin intraperitoneal introduction. In late terms of research (90th day), the high level of aberrations of chromosomes was retained. The most pronounced correction of cisplatin mutagenicity was achieved using a preliminary course of thiophan introduction.