Aerobic exercise training mitigates tumor growth and cancer-induced splenomegaly through modulation of non-platelet platelet factor 4 expression.

Exercise training reduces the incidence of several cancers, but the mechanisms underlying these effects are not fully understood. Exercise training can affect the spleen function, which controls the hematopoiesis and immune response. Analyzing different cancer models, we identified that 4T1, LLC, and CT26 tumor-bearing mice displayed enlarged spleen (splenomegaly), and exercise training reduced spleen mass toward control levels in two of these models (LLC and CT26). Exercise training also slowed tumor growth in melanoma B16F10, colon tumor 26 (CT26), and Lewis lung carcinoma (LLC) tumor-bearing mice, with minor effects in mammary carcinoma 4T1, MDA-MB-231, and MMTV-PyMT mice. In silico analyses using transcriptome profiles derived from these models revealed that platelet factor 4 (Pf4) is one of the main upregulated genes associated with splenomegaly during cancer progression. To understand whether exercise training would modulate the expression of these genes in the tumor and spleen, we investigated particularly the CT26 model, which displayed splenomegaly and had a clear response to the exercise training effects. RT-qPCR analysis confirmed that trained CT26 tumor-bearing mice had decreased Pf4 mRNA levels in both the tumor and spleen when compared to untrained CT26 tumor-bearing mice. Furthermore, exercise training specifically decreased Pf4 mRNA levels in the CT26 tumor cells. Aspirin treatment did not change tumor growth, splenomegaly, and tumor Pf4 mRNA levels, confirming that exercise decreased non-platelet Pf4 mRNA levels. Finally, tumor Pf4 mRNA levels are deregulated in The Cancer Genome Atlas Program (TCGA) samples and predict survival in multiple cancer types. This highlights the potential therapeutic value of exercise as a complementary approach to cancer treatment and underscores the importance of understanding the exercise-induced transcriptional changes in the spleen for the development of novel cancer therapies.

Consumo de medicamentos não padronizados na saúde indígena: uso racional? / Consumption of non-standard medication in indigenous health: rational usage?

Resumo O estudo analisou o consumo de medicamentos não padronizados na saúde indígena, enfatizando a racionalidade da farmacoterapia, por meio de um estudo transversal dos dados secundários, de 2018 e 2019, no Distrito Especial Sanitário de Saúde Indígena Minas Gerais/Espírito Santo. Esses medicamentos foram classificados pela Anatomical Therapeutic Chemical Classification. Para a comparação da origem de prescrição e da forma de aquisição, empregaram-se testes não paramétricos, avaliando o acesso a medicamentos. Verificou-se a racionalidade por meio do perfil de consumo e da opção terapêutica na lista de medicamentos padronizados. Foram consumidas 104.928 apresentações farmacêuticas, 66.967 (66%) eram para o trato alimentar e o metabolismo; 17.705 (17%) para o sistema nervoso; 12.961 (12%) para o sistema cardiovascular. Quanto aos medicamentos mais consumidos por regiões, 171 (90%) dos 190 apresentavam opção terapêutica. As prescrições foram mais provenientes do SUS. Encontraram-se diferenças na forma de aquisição dos medicamentos. O estudo apontou importante consumo de medicamentos não padronizados, podendo existir falhas na racionalidade terapêutica. Na saúde indígena, questões etnoculturais e sociais constituem desafios para o acesso aos medicamentos com uso racional.

Abstract The study analyzed the consumption of non-standard medication in the health of indigenous peoples, emphasizing the rationality of pharmacotherapy, by conducting a cross-sectional study of secondary data from 2018 and 2019 in the Minas Gerais/Espírito Santo Special Sanitary Indigenous Health Districts. These medicines were classified by Anatomical Therapeutic Chemical Classification. Non-parametric tests were applied to compare the origin of prescription and the form of acquisition, assessing access to medication. Rationality was verified through the consumption profile and the therapeutic option in the list of standardized medicines. A total of 104,928 pharmaceutical presentations were consumed, 66,967 (66%) for the alimentary tract and metabolism, 17,705 (17%) for the nervous system, and 12,961 (12%) for the cardiovascular system. With respect to medicines consumed per region, 171 (90%) out of 190 had a therapeutic option. Prescriptions were more from the SUS. Differences were found in the way the medicines were acquired. The study pointed to significant consumption of non-standard medicines, and there may be failings in therapeutic rationality. In indigenous health, ethnocultural and social issues are challenges to access to medicines with rational use.

Functional outcomes and satisfaction rates of sacral nerve stimulation in the treatment of one and multiples pelvic floor dysfunctions

Objective: Few studies have addressed the use of sacral nerve stimulation (SNS) in the treatment of patients with multiple pelvic floor dysfunctions (PFD). So, we evaluated the functional outcomes and level of satisfaction with SNS in selected patients with one or multiples PFD. Methods: A prospective database was used to collect information on eligible patients treated for PFD with SNS, and severity of symptoms was assessed with scores and satisfaction rates by visual analogue scale (VAS) at baseline and by the end of follow-up. Results: We recruited 70 patients, 98.6% of whom responded positively during the evaluation period (Global Response Assessment ≥ 50% for at least one type of PFD), resulting in the implantation of a permanent SNS device. Additionally, 49 of the patients (71%) had a single PFD (fecal incontinence [FI] = 38; constipation/obstructed defecation syndrome [C/ODS] = 11), while 20 (29%) had more than one PFD (double incontinence/n = 12; double incontinence + C/ODS/n = 8). All scores improved significantly between baseline (pre-SNS) and the end of follow-up (post-SNS), as did VAS in all groups (single and multiple PFD). The pre-SNS scores were higher in patients with a single PFD, including FI (Cleveland clinic Florida incontinence score [CCF-FI]) and C/ODS (Cleveland clinic constipation score [C-CCF] and the Renzi ODS score). The pre-SNS impact of VAS scores was similar in all groups (single and multiple PFD), but the VAS (post-SNS) was significantly lower (better response) for FI alone compared with multiple PFD. Conclusion: The SNS technique is an effective and safe option for patients with one or more PFD refractory to conservative measures. Response was positive for at least two PFD, based on reduced correspondent scores and satisfaction rate. (AU)

Audiovisual Methods in Research with Children: Interaction with Cameras and Videos / Métodos Audiovisuais em Pesquisas com Crianças: A Relação com Câmeras e Vídeos

ABSTRACT This article aims to analyze the use of audiovisual methods in research with children. Four children participated in this research about the presence of digital technologies in their lives in an urban childhood context. The methodological procedures were conversation circles and the production of videos. Results show the participants' interaction with the camera as an "other" that should be positioned, a transposition of meanings from audiovisual production to the research context, and the influence of the format of YouTubers' videos on the production of a video in a group. In addition to functioning as recording instruments, cameras, and audiovisual products imply specific modes of interaction and positioning among children.

RESUMO Este artigo objetiva refletir sobre a utilização de métodos audiovisuais em pesquisas com crianças. Participaram desta pesquisa quatro crianças sobre a presença das tecnologias digitais em suas vidas em um contexto de infância urbana. Os procedimentos metodológicos foram rodas de conversa e a produção de vídeos. Os resultados evidenciam a relação dos participantes com a câmera como um outro que precisa ser posicionado, a transposição de significados relacionados ao contexto de produções audiovisuais para o momento da pesquisa e a influência do formato dos vídeos de youtubers na produção de um vídeo em grupo. Para além de funcionarem como instrumentos de registro, as câmeras e os produtos audiovisuais suscitam modos específicos de interação e de posicionamento entre as crianças.

Análise de métodos de identificação humana para diferenciação de gêmeos monozigóticos: revisão de literatura / Analysis of human identification methods for differentiating monozygotic twins: a literature review

Devido à dificuldade de diferenciação entre gêmeos monozigóticos (GM) e as limitações existentes nos métodos, decorrentes das influências e similaridades genéticas, ambientais e faciais, a busca por métodos confiáveis que possam distinguir esses indivíduos geneticamente idênticos torna-se de grande importância para o campo forense. O objetivo deste trabalho foi realizar uma revisão da literatura do tipo narrativa a respeito dos métodos que ofereçam segurança para diferenciação entre GM. Constatou-se que a análise de pontos característicos individuais na impressão datiloscópicas e plantares, desenvolvimento dental, impressão labial, rugoscopia palatina, medidas faciais antropométricas, biometria ocular e por impressão vocal, padrões de cristas das unhas e de veias, seio frontal e impressão da língua são métodos eficazes para a identificação de GM. Ainda, superando a análise convencional de DNA, surgiu a análise genética por meio das variações sequenciais do genoma, denominado de Massively Parallel Sequencing que tornou possível distinguir gêmeos monozigóticos. Além dos métodos primários de identificação humana que permitem a diferenciação de GM, como a papiloscopia e odontologia, diversos métodos para diferenciação de GM estão descritos na literatura, e cada qual possui suas vantagens e limitações no sentido de propiciar ao perito a melhor informação no sentido de que GM sejam adequadamente diferenciados

A number of limitations can be found in the methods in identifying monozygotic twins (MT) due to genetic influences, facial and environmental similarities, the search for reliable methods to identify genetically identical individuals has become a great importance for Forensic Science. The objective of this work was to conduct a literature review in search of methods that offer attested identification among MT. It was found that the analysis of individual characteristic points in fingerprint and plantar impressions, dental development, lip impression, palatal rugoscopy, anthropometric facial measurements, ocular and voice impression biometry, patterns of nail ridges and veins, sinus Front and tongue print are effective methods for MT identification. Also, surpassing the conventional analysis of DNA, genetic analysis emerged through the sequential variations of the genome, called Massively Parallel Sequencing, which made it possible to distinguish monozygotic twins. In addition to the primary methods of human identification that allow the differentiation of MT, such as papiloscopy and dentistry, several methods for differentiating MT are described in the literature, and each one has its advantages and limitations in the sense of providing the expert with the best information in terms of that GM are properly differentiated

Reclamações fundamentadas de âmbito odontológico no PROCON do estado da Paraíba / Grounded dental complaints in PROCON of the state of Paraíba

Introdução: A relação entre pacientes e profissionais, vista como contratação de prestação serviços, em conjunto com o advento do Código de Defesa do Consumidor, traz como consequência o maior conhecimento sobre direitos por parte dos consumidores, os quais passaram, com mais frequência, a reclamar e a buscar ressarcimentos pelos possíveis danos causados. Objetivo: Analisar as reclamações fundamentadas de âmbito odontológico obtidas do PROCON da Paraíba. Material e Métodos: Estudo observacional documental, com universo composto de todos os processos de reclamações fundamentadas oriundos dos relatórios disponibilizados, através do Sistema Nacional de Informações de Defesa do Consumidor, pelo PROCON da Paraíba, sendo a amostra representada pelas reclamações de âmbito odontológico entre 2015 e 2021, as quais foram analisadas de forma descritiva. Resultados: Foram registradas 13.893 reclamações fundamentadas, sendo 66 de âmbito odontológico (0,47%); o ano com maior percentual encontrado foi o de 2020 (0,73%); das reclamações de âmbito odontológico, houve maior frequência de casos relacionados aos planos odontológicos (66,66%), seguidos das clínicas ou consultórios odontológicos (31,81%), restando apenas uma reclamação registrada na categoria cirurgiões-dentistas (1,51%); os principais motivos relatados foram problemas com o Serviço de Atendimento ao Consumidor (SAC), cobrança indevida ou não autorizada e publicidade enganosa; e houve predominância de atendimentos às reivindicações. Conclusão: Não houve constância de crescimento das reclamações de âmbito odontológico obtidas do PROCON/PB entre os anos de 2015 a 2021, os planos odontológicos constituíram seu maior alvo, tendo como principal motivo a reclamação contra o SAC

Introduction: The relationship between patients and professionals, seen as contracting services, together with the advent of the Consumer Protection Code, brings as a consequence greater knowledge about rights on the part of consumers, who have more often claim and seek compensation for possible damages caused. Objective: To analyze substantiated dental complaints obtained from PROCON in Paraíba. Material and Methods: Observational documentary study, with a universe composed of all substantiated complaints processes arising from the reports made available, through the National Consumer Protection Information System, by PROCON of Paraíba, with the sample represented by dental complaints between 2015 and 2021, which were analyzed in a descriptive way. Results: 13,893 substantiated complaints were registered, of which 66 were related to dentistry (0.47%); the year with the highest percentage found was 2020 (0.73%); of dental complaints, there was a higher frequency of cases related to dental plans (66.66%), followed by dental clinics or offices (31.81%), leaving only one complaint registered in the dental surgeons category (1.51% ); the main reasons reported were problems with the Customer Service (SAC), improper or unauthorized billing and misleading advertising; and there was a predominance of requests for assistance. Conclusion: There was no constant growth in dental complaints obtained from PROCON/PB between the years 2015 to 2021, dental plans were its biggest target, with the main reason being the complaint against the SAC

Torus mandibular e palatino predisponentes em um grupo familiar: fatores genéticos e ambientais, relato de uma série de casos / Mandibular and palatine torus predisposing in a family group: genetic and environmental factors, report of a series of cases / Torus mandibular y palatino predisponente en un grupo familiar: factores genéticos y ambientales, informe de una serie de casos

As exostoses mais conhecidas são o Torus palatino e mandibular, que se desenvolvem a partir do crescimento benigno da cortical óssea, localizadas respectivamente na linha média palatina e superfície lingual de caninos e pré-molares, podendo ser unilaterais ou bilaterais. Esse trabalho tem como objetivo o estudo de um grupo familiar que apresentaram o Torus como característica em comum, buscando confirmar a presença e prevalência das características clínicas multifatoriais listadas na literatura, possibilitando o diagnóstico da etiologia e assim traçando um plano de tratamento individual, se necessário. Como metodologia, foi realizada uma triagem com cada membro familiar na clínica Odontológica da Faculdade Sete Lagoas - FACSETE, descartando a participação no estudo os membros que não apresentaram o Torus. Os indivíduos que apresentaram indicação da remoção cirúrgica foram encaminhados para clínica de cirurgia da própria instituição. Concluímos, portanto, com este estudo que fatores genéticos e ambientais colaboram como fator etiológico mais predominantes para surgimento do Torus no grupo familiar estudado... (AU)

The best known exostosis are the palatine and mandibular Torus, which develop from the benign growth of the cortical bone, located respectively in the midpalatal line and lingual surface of canines and premolars, and maybe unilateral or bilateral. This work aims to study a family group that had Torus as a common feature, confirming the presence and prevalence of multifactorial clinical features listed in the literature, enabling the diagnosis of etiology and thus outlining an in dividual treatment plan, if necessary. As a methodology, a screening was performed by each family member at the Dental Clinic of Facul dade Sete Lagoas - FACSETE, discarding the participation in the study for members who did not present Torus. Individuals who indicated surgical removal were referred to the institution's own surgery clinic. Therefore, we conclude with this study that genetic and environmental factors collaborate as the most predominant etiological factor for the emergence of Torus in the studied family group... (AU)

Las exostosis más conocidas son el Torus palatino y el Torus mandibular, las cuales se desarrollan a partir del crecimiento benigno del hueso cortical y están ubicadas respectivamente en la línea medio palatina y en la cara lingual de los caninos y de los premolares que pueden ser unilaterales o bilaterales. Este proyecto tiene como objetivo el estudio de un grupo familiar que presentó en sus características comunes el Torus, buscando confirmar la presencia y el predominio de las características clínicas multifactoriales listadas en la literatura, lo que hizo posible el diagnóstico de la etiología y diseñar un plan de tratamiento individual, caso necesiten. Como la metodología fue hecho una selección con los miembros de la familia en la clínica odontologica de la Faculdade Sete Lagoas - FACSETE, quitando de los estudios las personas de la familia que no presentaron el Torus. Los individuos que presentaron indicación de extirpación quirúrgica fueron enviados a la clínica de cirugía de la escuela. Concluimos con este estudio que los factores genéticos y ambientales colaboraron como los factores etiológicos más predominantes para la aparición del Torus en la familia analizada... (AU)

Identification of early biomarkers in saliva in genetically engineered mouse model C(3)1-TAg of breast cancer.

Breast cancer is one of leading causes of death worldwide in the female population. Deaths from breast cancer could be reduced significantly through earlier and more efficient detection of the disease. Saliva, an oral fluid that contains an abundance of protein biomarkers, has been recognized as a promising diagnostic biofluid that is easy to isolate through non-invasive techniques. Assays on saliva can be performed rapidly and are cost-effective. Therefore, our work aimed to identify salivary biomarkers present in the initial stages of breast cancer, where cell alterations are not yet detectable by histopathological analysis. Using state-of-the-art techniques, we employed a transgenic mouse model of mammary cancer to identify molecular changes in precancerous stage breast cancer through protein analysis in saliva. Through corroborative molecular approaches, we established that proteins related to metabolic changes, inflammatory process and cell matrix degradation are detected in saliva at the onset of tumor development. Our work demonstrated that salivary protein profiles can be used to identify cellular changes associated with precancerous stage breast cancer through non-invasive means even prior to biopsy-evident disease.

Pathogenicity and Growth Conditions Modulate Fonsecaea Extracellular Vesicles' Ability to Interact With Macrophages.

Chromoblastomycosis (CBM) is a chronic cutaneous and subcutaneous mycosis caused by black, dimorphic, and filamentous fungi of the Herpothrichiellaceae family, such as species of the genus Fonsecaea. These fungi can switch between the saprophytic forms (conidia and hyphae) and the pathogenic form, the muriform cells (MCs), which is considered an essential mechanism for fungal virulence. Nearly all types of cells can produce membranous structures formed by a lipid bilayer that communicate extracellularly with other cells, known as "extracellular vesicles" (EVs), which may act as virulence factors, as observed for several species of pathogenic fungi. Our findings demonstrated for the first time that F. pedrosoi, F. nubica, and F. erecta produce EVs in response to nutritional conditions. The EVs varied in sterol and protein contents, size, and morphology. Moreover, the EVs induced different cytokine and nitric oxide release patterns by bone marrow-derived macrophages (BMDMs). The EVs activated IL-1ß production, possibly acting as the first signal in inflammasome activation. Unlike the pathogenic species, the EVs isolated from F. erecta did not significantly stimulate TNF and IL-10 production in general. Overall, these results demonstrated that different species of Fonsecaea produce EVs capable of modulating pro- and anti-inflammatory cytokine and nitric oxide production by BMDMs and that growth conditions affected the immunomodulatory capacities of the EVs as well as their size, content, and morphology.

Salivary Proteome, Inflammatory, and NETosis Biomarkers in Older Adult Practitioners and Nonpractitioners of Physical Exercise.

Since aging has been associated with increased production of inflammatory biomarkers, the ability to monitor older adults repeatedly is highly desirable, and saliva is an interesting biofluid for the search of biomarkers, as it is easily accessible in a noninvasive manner. However, given the incipient knowledge of salivary biomarkers in aging and its relationship to physical exercise, the present study is aimed at evaluating the protein expression and the levels of inflammatory and NETosis biomarkers in the saliva of practitioners (PE) and nonpractitioners (NPE) of physical exercise older adults. Six (6) practitioner and 4 nonpractitioner older adults were enrolled in this study. Unstimulated whole saliva was collected for analysis of the proteome by label-free mass spectrometry, as well as of the inflammatory status by evaluation of C-reactive protein (CRP), vascular endothelial growth factor (VEGF), and cytokines (TNF-α, interleukin- (IL-) 1ß, and IL-8), while NETosis was assessed by myeloperoxidase (MPO) and neutrophil elastase. Regarding oral health, the decayed, missing, and filled teeth (DMF-T) index, bleeding on probing, suppuration, and probing depth measurement (mm) were evaluated. In addition, functional capacity was investigated using the General Physical Fitness Index (GPFI). In relation to the proteome analysis, 93 and 143 proteins were found exclusively in the PE and NPE groups, respectively; 224 proteins were common to both groups. Among these proteins, 10 proteins showed statistical difference (p < 0.05) between the groups: alpha-2-macroglobulin, component 3 of the complement, serotransferrin, and protein soluble in brain acid 1 were less expressed, while lactotransferrin, alpha-amylase 1, S100-A8, S100-A9, lactoperoxidase, and galectin-3 binding protein were more expressed in the PE group. No differences between groups were observed in the analysis of inflammatory and NETosis biomarkers. This study shows the potential utility of saliva for detecting protein biomarkers in a noninvasive biological sample of the elderly population.

ß2 Integrin-Mediated Susceptibility to Paracoccidioides brasiliensis Experimental Infection in Mice.

The earliest interaction between macrophages and Paracoccidioides brasiliensis is particularly important in paracoccidioidomycosis (PCM) progression, and surface proteins play a central role in this process. The present study investigated the contribution of ß2 integrin in P. brasiliensis-macrophage interaction and PCM progression. We infected ß2-low expression (CD18low) and wild type (WT) mice with P. brasiliensis 18. Disease progression was evaluated for fungal burden, lung granulomatous lesions, nitrate levels, and serum antibody production. Besides, the in vitro capacity of macrophages to internalize and kill fungal yeasts was investigated. Our results revealed that CD18low mice infected with Pb18 survived during the time analyzed; their lungs showed fewer granulomas, a lower fungal load, lower levels of nitrate, and production of high levels of IgG1 in comparison to WT animals. Our results revealed that in vitro macrophages from CD18low mice slowly internalized yeast cells, showing a lower fungal burden compared to WT cells. The migration capacity of macrophages was compromised and showed a higher intensity in the lysosome signal when compared with WT mice. Our data suggest that ß2 integrins play an important role in fungal survival inside macrophages, and once phagocytosed, the macrophage may serve as a protective environment for P. brasiliensis.

Impacto da escolaridade na violência infantojuvenil na cidade de João Pessoa ­ PB ­ Brasil / Impact of schooling on child and youth violence in João Pessoa -PB -Brazil

Introdução: A violência contra crianças e adolescentes constitui problema de saúde pública. Objetivo: Avaliar o impacto da escolaridade em vítimas de violência infantojuvenil submetidas a exame traumatológico no Núcleo de Medicina e Odontologia Legal (NUMOL) da cidade de João Pessoa/PB ­ Brasil, em 2017. Material e Métodos: Estudo documental, transversal, analítico, com técnica de documentação indireta, a partir da análise dos laudos periciais produzidos em 2017. Variáveis analisadas: idade, ocupação da vítima (G1= não estudante e G2 = estudante), local da agressão, energia e instrumento lesivo, localização e natureza da lesão e relação vítima/agressor. A associação entre as variáveis qualitativas foi avaliada pelos testes de Qui-quadrado e Exato de Fischer (nível de significância de 5,0%). Resultados: Analisaram-se 384 laudos, com tempo médio entre agressão e denúncia de 8,95 dias e via pública (19,5%) como principal local de ataque. A energia mecânica (G1 = 99,0% e G2 = 99,5%) e o instrumento contundente (G1 = 90,5% e G2 = 88,1%) foram os mais utilizados; membros (G1 = 55,0% e G2 = 54,4%) e face (G1 = 34,6% e G2 = 41,5%) as regiões mais afetadas. Prevaleceram lesões leves (G1 = 94,7% e G2 = 90,0%) e agressões por pessoas estranhas à vítima (G1 = 65,4% e G2 = 41,4%). Conclusão: Ausência de diferença significativa entre os grupos (G1 = 49,7% e G2 = (50,3%), maioria com idades entre 13 a 18 anos (80,4%), o estranho o principal agressor e os instrumentos contundentes mais utilizados para provocar lesões leves em membros e face.

ß2-Adrenergic Signaling Modulates Mitochondrial Function and Morphology in Skeletal Muscle in Response to Aerobic Exercise.

The molecular mechanisms underlying skeletal muscle mitochondrial adaptations induced by aerobic exercise (AE) are not fully understood. We have previously shown that AE induces mitochondrial adaptations in cardiac muscle, mediated by sympathetic stimulation. Since direct sympathetic innervation of neuromuscular junctions influences skeletal muscle homeostasis, we tested the hypothesis that ß2-adrenergic receptor (ß2-AR)-mediated sympathetic activation induces mitochondrial adaptations to AE in skeletal muscle. Male FVB mice were subjected to a single bout of AE on a treadmill (80% Vmax, 60 min) under ß2-AR blockade with ICI 118,551 (ICI) or vehicle, and parameters of mitochondrial function and morphology/dynamics were evaluated. An acute bout of AE significantly increased maximal mitochondrial respiration in tibialis anterior (TA) isolated fiber bundles, which was prevented by ß2-AR blockade. This increased mitochondrial function after AE was accompanied by a change in mitochondrial morphology towards fusion, associated with increased Mfn1 protein expression and activity. ß2-AR blockade fully prevented the increase in Mfn1 activity and reduced mitochondrial elongation. To determine the mechanisms involved in mitochondrial modulation by ß2-AR activation in skeletal muscle during AE, we used C2C12 myotubes, treated with the non-selective ß-AR agonist isoproterenol (ISO) in the presence of the specific ß2-AR antagonist ICI or during protein kinase A (PKA) and Gαi protein blockade. Our in vitro data show that ß-AR activation significantly increases mitochondrial respiration in myotubes, and this response was dependent on ß2-AR activation through a Gαs-PKA signaling cascade. In conclusion, we provide evidence for AE-induced ß2-AR activation as a major mechanism leading to alterations in mitochondria function and morphology/dynamics. ß2-AR signaling is thus a key-signaling pathway that contributes to skeletal muscle plasticity in response to exercise.

Skene's Gland Derivatives in the Female Genital Tract and Cervical Adenoid Basal Carcinoma are Consistently Positive With Prostatic Marker NKX3.1.

Cervical ectopic prostatic tissue and vaginal tubulosquamous polyp are rare lesions which exhibit variable, and often focal, immunohistochemical expression with traditional prostatic markers [prostate-specific antigen and prostatic acid phosphatase (PSAP)]. These lesions are thought to arise from periurethral Skene's glands, the female equivalent of prostatic glands in the male. Adenoid basal carcinoma is a rare and indolent cervical neoplasm. Expression of the prostatic marker NKX3.1 in ectopic prostatic tissue and tubulosquamous polyp has been reported but no studies have examined immunoreactivity with this marker in adenoid basal carcinoma. We stained 19 cases [adenoid basal carcinoma (n=6), cervical ectopic prostatic tissue (n=11), and vaginal tubulosquamous polyp (n=3); 1 case contained both adenoid basal carcinoma and ectopic prostatic tissue] with NKX3.1. In all cases, the glandular component of these lesions exhibited diffuse nuclear immunoreactivity while normal endocervical glands were negative. Prostate-specific antigen was positive in 4 of 9 and 0 of 3 cases of ectopic prostatic tissue and tubulosquamous polyp, respectively, while PSAP was positive in 3 of 4 and 2 of 2 cases of ectopic prostatic tissue and tubulosquamous polyp respectively; 3 of 5 cases of adenoid basal carcinoma tested were focally positive with PSAP and all 5 were negative with prostate-specific antigen. While the specificity of NKX3.1 should be investigated in future studies, positivity with this marker may be useful in diagnosing these uncommon lesions. NKX3.1 appears a more sensitive marker of ectopic prostatic tissue and tubulosquamous polyp than traditional prostatic markers and positive staining provides further support that these lesions exhibit "prostatic" differentiation and are of Skene's gland origin. NKX3.1 and PSAP positivity in adenoid basal carcinoma raises the possibility of an association with benign glandular lesions exhibiting prostatic differentiation and we critically discuss the possible association.

Mind Health Before and After Total Knee Arthroplasty.

Objective To assess the preoperative and postoperative outcomes of patients diagnosed with severe knee osteoarthritis who underwent a total knee arthroplasty (TKA) using a mobile-bearing implant with a rotating platform and removing the posterior cruciate ligament. The present study focused on the outcomes relative to depression, pain, functional limitations, and fall episodes. Methods The Lequesne questionnaire was used to assess pain and functional limitations before and after TKA. In addition, the geriatric depression scale (GDS) was also used. Episodes of falls before and after the surgery were estimated. Results The mean Lequesne score before the surgery was 15.95, and that after surgery was 6.5. This finding was statistically significant ( p < 0.001). The mean GDS score before the surgery was 7.43, and that after TKA was 2.22 ( p < 0.001). The mean number of fall occurrences before the procedure, over a 1-year period, was 1.22, and that after TKA was 0.27 ( p = 0.004). A direct relationship was found between the Lequesne scores before the surgery and the GDS scores ( p = 0.004). Conclusions Total knee arthroplasty resulted in the improvement of pain and functional limitation, decrease or disappearance of the depressive condition, and decrease of fall rates in the evaluated patients.

Mind Health Before and After Total Knee Arthroplasty / Saúde mental antes e depois da artroplastia total do joelho

Abstract Objective To assess the preoperative and postoperative outcomes of patients diagnosed with severe knee osteoarthritis who underwent a total knee arthroplasty (TKA) using a mobile-bearing implant with a rotating platform and removing the posterior cruciate ligament. The present study focused on the outcomes relative to depression, pain, functional limitations, and fall episodes. Methods The Lequesne questionnaire was used to assess pain and functional limitations before and after TKA. In addition, the geriatric depression scale (GDS) was also used. Episodes of falls before and after the surgery were estimated. Results The mean Lequesne score before the surgery was 15.95, and that after surgery was 6.5. This finding was statistically significant (p < 0.001). The mean GDS score before the surgery was 7.43, and that after TKA was 2.22 (p < 0.001). The mean number of fall occurrences before the procedure, over a 1-year period, was 1.22, and that after TKA was 0.27 (p = 0.004). A direct relationship was found between the Lequesne scores before the surgery and the GDS scores (p = 0.004). Conclusions Total knee arthroplasty resulted in the improvement of pain and functional limitation, decrease or disappearance of the depressive condition, and decrease of fall rates in the evaluated patients.

Resumo Objetivo Avaliar os resultados pré e pós-operatórios em relação à depressão, dor, limitações funcionais e episódios de queda em pacientes diagnosticados com osteoartrite (OA) grave do joelho submetidos a uma artroplastia total do joelho (ATJ), usando um implante móvel com uma plataforma rotativa e removendo o ligamento cruzado posterior. Métodos O questionário de Lequesne foi utilizado para avaliar a dor e as limitações funcionais antes e após a ATJ. Além disso, a escala de depressão geriátrica (EDG) também foi utilizada. Os episódios de quedas antes e após a cirurgia foram estimados. Resultados O escore médio de Lequesne antes da cirurgia foi de 15,95 e após a cirurgia foi de 6,5. Esse resultado foi estatisticamente significativo (p < 0,001). O escore médio da EDG antes da cirurgia foi de 7,43 e após a ATJ foi de 2,22 (p < 0,001). O número médio de ocorrências de queda antes do procedimento, em um período de 1 ano, foi de 1,22 e após a ATJ foi de 0,27 (p = 0,004). Foi encontrada uma relação direta entre os escores de Lequesne antes da cirurgia e os escores da EDG (p = 0,004). Conclusões A ATJ resultou em melhora da dor e limitação funcional, diminuição ou desaparecimento da condição depressiva e diminuição das taxas de queda nos pacientes avaliados.

Perfil das Vítimas de Suicídio em Município da Paraíba/Brasil / Profile of Suicide Victims in a Municipality of Paraíba / Brazil

Objetivo: Traçar o perfil dos óbitos das vítimas de suicídio necropsiadas no Núcleo de Medicina e Odontologia Legal do Instituto de Polícia Científica do Estado da Paraíba do Muni-cípio de Guarabira, no período de 2016 a 2017. Metodologia: Pesquisa documental e descritiva com abordagem quantitativa. A amostra foi composta por 47 laudos de óbitos por suicídio. Resultados: Das 47 mortes por suicídio, 76,6% eram do sexo masculino e 23,4% do sexo feminino. A média de idade foi de 44,3 anos com desvio padrão de 19,8, não existindo diferença quanto a idade para o sexo masculino e o feminino (p=0,223). Quanto ao estado civil, 55,3% eram solteiros, seguidos de casados (29,8%), 4,3% eram viúvos, 6,4% não tinham esta informação e 4,3% era ignorado. Do total de suicídios, 68,1% das vítimas foi enforcamento, seguido de 23,4% de envenena-mento, 4,3% arma de fogo, 2,1% precipitação de altura e 2,1% automutilação como meio para realizar o seu intuito. Conclusão: As vítimas mais frequentes pertenciam ao sexo masculino, adultos jovens e solteiros. A atividade laboral que mais se mostrou presente foi a agricultura, com meio mais utilizado o enforcamento, seguido pelo envenenamento. Os meses que existiram mais vítimas foram maio, agosto e setembro, com a segunda-feira e quinta-feira mais prevalentes. Espera-se que as características observadas dessas vítimas possibilitem a tomada de intervenções , com o objetivo de identificar os grupos de risco e estabelecer políticas de prevenção. (AU)

Objective: To outline the profile of deaths of suicide victims necropsied at the Center for Medicine and Forensic Dentistry of the Paraíba State Scientific Police Institute in Guarabira, from 2016 to 2017. Methodology: This is a documentary and descriptive research with a quantitative approach. The sample consisted of 47 reports of deaths from suicide. Results: Results showed that of the 47 suicide deaths, 76.6% were male and 23.4% female. The mean age was 44.34 years with a standard deviation of 19.83 in the total group, and no difference in the age of males and females (p = 0.223). Regarding marital status, 55.3% were single, followed by 29.8% were married, 4.3% were widowed, 6.4% did not have this information and 4.3% were ignored. Regarding the method to carry out the suicide, 68.1% of the victims were hanged, followed by 23.4% were poisoned, 4.3% were by firearm, 2.1% were by hight precipitation and 2.1% self-mutilation. Conclusion: The most frequent victims were male, young and single adults. The labor activity that was most present was agriculture, with hanging being the most used method, followed by poisoning. The months with the most casualties were May, August, and September, with the most prevalent days being Monday and Thursday. It is hoped that the observed characteristics will be able to enable effective in-terventions in order to identify suicide risk groups and establish prevention politics. (AU)

De espectadora a criadora: imaginação, faz de conta e mídia televisiva no desenvolvimento infantil / De espectadora a creadora: imaginación, juego y medio televisivo en el desarrollo infantil / From spectator to creator: imagination, pretend play and television media on child development

Resumo Este artigo objetiva analisar, à luz da perspectiva histórico-cultural, os sentidos produzidos pelas crianças sobre a mídia televisiva em contexto de faz de conta. Para tanto, foi realizado um estudo empírico em uma escola pública do Distrito Federal, em uma turma do primeiro ano do Ensino Fundamental com 26 alunos (entre cinco e sete anos). O desenho metodológico estruturou-se em duas etapas: observação de situações lúdicas espontâneas das crianças e oficinas lúdicas com temas referentes ao universo midiático-televisivo. Ambos os momentos foram registrados em diário de campo e em videogravações; e as informações construídas foram transcritas e submetidas a uma análise microgenética, por meio da qual foi possível a construção de categorias de análise referentes aos diferentes modos que as crianças expressam, em suas atividades criadoras, os sentidos que possuem sobre a mídia televisiva. Para além do lugar de espectadoras, elas atuam como protagonistas em seus processos de desenvolvimento.

Resumen Este artículo tiene como objetivo analizar, a la luz de la perspectiva histórico-cultural, los significados producidos por las/os niñas/os sobre el medio televisivo durante episodios de juego. Para ello, se realizó un estudio empírico en una escuela pública del Distrito Federal, en una clase del primer año de primaria con 26 alumnos (entre cinco y siete años). El diseño metodológico de la investigación se estructuró en dos etapas: observación de situaciones lúdicas espontáneas de niños y talleres lúdicos con temas relacionados con el universo de los medios. Ambos momentos fueron registrados en el diario de campo y en grabaciones de video; y los datos construidos fueron transcritos y sometidos a un análisis microgenético, a través del cual fue posible construir categorías de análisis que se refirieron a las diferentes formas en que los niños expresan, en sus actividades creativas, los significados sobre el medio televisivo. Además de ser espectadores, las/los niñas/os actúan como protagonistas en sus procesos de desarrollo.

Abstract Grounded on the cultural-historical perspective, this article aims to analyze the meanings produced by children about television media during the context of pretend play. For this purpose, an empirical study was carried out in a public school in the Federal District, in a class of elementary school's first year with 26 students (from five to seven years old). The research's methodological design was structured in two phases: observation of the children's spontaneous playful situations and ludic workshops with themes related to the television media universe. Both moments were recorded in a field journal as well as in video format. The constructed data was transcribed and subjected to a micro genetic analysis. The analysis led to the construction of analytic categories regarding the various ways that children express the meanings they produce about television media in their creative activities. More than being spectators, children act as protagonists in their development processes.

Características Antropométricas do Palato Ósseo de uma Amostra Brasileira de Esqueletos Identificados / Anthropometric Characteristics of the Bony Palate in a Brazilian Sample of Identified Skeletons

Objetivo: Este estudo teve como objetivo descrever as carac-terísticas antropométricas do palato ósseo de uma amostra brasileira de esqueletos identificados Metodologia: Foi desen-volvido um estudo de corte transversal, por meio da análise de 178 crânios secos humanos, oriundos do Arquivo de Ossadas do Departamento de Odontologia Social da Faculdade de Odon-tologia de Piracicaba (FOP/UNICAMP), São Paulo. Utilizando paquímetro digital, foram realizadas medições lineares que incluíam forame incisivo à espinha nasal posterior (FI-ENP), forame palatino maior direito ao forame palatino maior esquer-do (FPMD-FPME), forame incisivo ao forame palatino maior direito (FI-FPMD) e forame incisivo ao forame palatino maior esquerdo (FI-FPME). Os testes t-Student, F(ANOVA), Tukey, LSD, Kruskal Wallis e Pearson, com nível de significância de 5,0%. Resultados: Do total de crânios analisados, 54,5% eram do sexo masculino, com mediana de idade de 56 anos. Todas as medidas analisadas apresentaram dimorfismo sexual, onde homens possuíram maiores dimensões do que o sexo oposto (p<0,001). Do total de palatos estudados, 104 eram pertencen-tes a indivíduos brancos, 49 a miscigenados e 25 a negros. As medidas FI-ENP, FPMD-FPME e FI-FPME apresentaram-se maiores em negros, seguidos dos miscigenados e brancos. Conclusão: Conclui-se que as características do palato ósseo podem ser aplicadas como maior confiabilidade para a estima-tiva do sexo na amostra brasileira estudada. (AU)

Objective: This study aimed to describe the anthropometric characteristics of the bony palate in a Brazilian sample of identified skeletons. Methodology: This was a cross-sectional study of 178 human skulls from the Laboratory of Forensic Physical Anthropology at Piracicaba Dental School, University of Campinas, São Paulo. A digital pachymeter was used to perform linear measurements from the incisive foramen to the posterior nasal spine (IF-PNS); right greater palatine foramen to left greater palatine foramen (RGPF-LGPF); incisive foramen to the right greater palatine foramen (IF-RGPF); and incisive foramen to the left greater palatine foramen (IF-LMPF). The data were submitted to Student's t, F (ANOVA), Tukey, LSD, Kruskal Wallis and Pearson tests, with a significance level of 5.0%. Results: Of the total skulls analyzed, 54.5% were from males, with a median age of 56 years. All the study measures presented sexual dimorphism, in which males had larger dimen-sions than females (p<0.001). Of the total palates studied, 104 were from white individuals, 49 were from mixed-race and 25 from black individuals. The IF-PNS, RGPF-LGPF and IF-LGPF measurements were greater in black individuals, followed by mixed-race and white individuals. Conclusion: In conclusion, the characteristics of the bony palate can be applied with higher reliability to estimate sex in Brazilian sample studied.(AU)

Aplicabilidade do índice forame magno em crânios humanos de indivíduos do nordeste brasileiro / Applicability of the foramen magnum index on human skulls of individuals from northeastern Brazil

Introdução: O Índice Forame Magno (IFM) é um método secundário para o estabelecimento do sexo no processo de identificação humana. Objetivo: Averiguar a aplicabilidade do IFM para a determinação do sexo em uma amostra brasileira. Material e Método: Estudo cego e transversal, com amostra composta por 209 crânios pertencentes ao Centro de Estudos em Antropologia Forense da Faculdade de Odontologia de Pernambuco/Universidade de Pernambuco (CEAF/FOP/UPE). Com o uso de paquímetro digital de precisão, foram realizadas medidas correspondentes à largura e ao comprimento do forame magno, aplicando-as à fórmula IFM = largura/comprimento x 100. O sexo foi classificado de acordo com tabela de referência para o índice supracitado. Para a análise estatística utilizaram-se os testes T-Student e Qui-Quadrado de Pearson, com nível de significância de 5,0%. Resultados: Todas as variáveis quantitativas apresentaram diferenças estatisticamente significantes para os sexos (p<0,05), porém não houve discrepâncias entre os mesmos quanto à utilização do IFM (p=0,965). O acerto do IFM para o sexo masculino (99,1%), mas para o sexo feminino este método não classificou nenhum crânio corretamente. Conclusão: As dimensões do Forame Magno apresentaram dimorfismo sexual, porém o IFM não deve ser utilizado como metodologia para a determinação do sexo da amostra brasileira estudada.

Introduction: The Foramen Magnum Index (IMF) is a secondary method for the establishment of the sex in the process of human identification. Objective: To investigate the applicability of FMI to the determination of sex in a Brazilian sample. Material and Method: Blind and transversal study, with sample composed of 209 skulls belonging to the Forensic Anthropology Center of the Dentistry School of Pernambuco/Universidade de Pernambuco (CEAF/FOP/UPE). With the aid of a digital caliper, measurements were made on the width and length of the Foramen Magnum, applying them to the FMI formula FMI = width/length x 100. The sex was classified according with the reference table to the above mentioned index. For the statistical procedures, were used the testes Student T and Pearson's Chi-Square, with a significance level of 5%. Results: All the quantitative variables presented statistically significant differences for the sexes (p<0,05), but there were no discrepancies between them regarding the use of the FMI (p=0.965). The concordances of the FMI for males (99.1%) were higher than those of females, which did not have any cranial classification correctly. Conclusion: The FMI should not be used as a single methodology for determining the sex of the Brazilian sample studied