In vivo solid phase microextraction for therapeutic monitoring and pharmacometabolomic fingerprinting of lung during in vivo lung perfusion of FOLFOX.

In vivo lung perfusion (IVLP) is a novel isolated lung technique developed to enable the local, in situ administration of high-dose chemotherapy to treat metastatic lung cancer. Combination therapy using folinic acid (FOL), 5-fluorouracil (F), and oxaliplatin (OX) (FOLFOX) is routinely employed to treat several types of solid tumours in various tissues. However, F is characterized by large interpatient variability with respect to plasma concentration, which necessitates close monitoring during treatments using of this compound. Since plasma drug concentrations often do not reflect tissue drug concentrations, it is essential to utilize sample-preparation methods specifically suited to monitoring drug levels in target organs. In this work, in vivo solid-phase microextraction (in vivo SPME) is proposed as an effective tool for quantitative therapeutic drug monitoring of FOLFOX in porcine lungs during pre-clinical IVLP and intravenous (IV) trials. The concomitant extraction of other endogenous and exogenous small molecules from the lung and their detection via liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) enabled an assessment of FOLFOX's impact on the metabolomic profile of the lung and revealed the metabolic pathways associated with the route of administration (IVLP vs. IV) and the therapy itself. This study also shows that the immediate instrumental analysis of metabolomic samples is ideal, as long-term storage at -80 °C results in changes in the metabolite content in the sample extracts.

Microfluidic amperometric immunosensor based on porous nanomaterial towards claudin7 determination for colorectal cancer diagnosis.

In this work, we present a microfluidic amperometric immunosensor for cancer biomarker claudin7 (CLD7) determination in circulating extracellular vesicles (EVs) as well as its validation in colorectal cancer (CC) patients. The device is based on synthetized nanosized MIL-125-NH2 particles, covalently anchored to the central channel of the microfluidic immunosensor. This nanomaterial was employed as efficient platform for anti-CLD7 monoclonal antibodies immobilization for specifically recognize and capture CLD7 in EVs samples. Afterwards, the amount of this trapped CLD7 was quantified by HRP-conjugated anti-CLD7-antibody. HRP reacted with its enzymatic substrate in a redox process which resulted in the appearance of a current whose magnitude was directly proportional to the level of CLD7 in the sample. This immunosensor, under optimum conditions, gave the limit of detection for CLD7 of 0.1 pg mL-1, with a wide linear range from 2 to 1000 pg mL-1. The results reported herein open up the use of porous open framework platforms for sensing applications for biomedicine and diagnosis.

Validación de la Escala de Valoración de Agencia de Autocuidado Revisada en una muestra colombiana / Validation of the Self-Care Agency Rating Scale-Revised in a Colombian Sample / Validação da Self-Care Agency Rating Scale-Revised em uma amostra colombiana

Introducción: la Escala de Valoración de Agencia de Autocuidado Revisada es un instrumento diseñado para valorar el autocuidado de la salud en población clínica; sin embargo, se desconocen estudios sobre sus propiedades psicométricas en población general no-clínica colombiana. Objetivo: validar la Escala de Valoración de Agencia de Autocuidado Revisada en una muestra de 306 participantes voluntarios en Colombia. Método: estudio de diseño metodológico psicométrico realizado entre octubre y diciembre de 2021. Para el análisis de la validez de estructura se aplicaron técnicas de análisis factorial, exploratorio y confirmatorio. De manera complementaria, se evaluó la validez de contenido a través de un panel de expertos. Finalmente, como evidencia de la confiabilidad del instrumento se analizó su consistencia interna mediante el coeficiente Omega de McDonald. Resultados: los hallazgos muestran que el ASA-R muestra una estructura unidimensional que explica el 61,99 % de la varianza total. La valoración de la relevancia, claridad, precisión y comprensión de los ítems tuvo resultados cuyo promedio osciló entre una V de Aiken de 0,79 y 0,99. La consistencia interna mostró un índice satisfactorio para la escala total (ω=0,93). Conclusiones: la versión del ASA-R presentada en este artículo posee propiedades métricas adecuadas y se recomienda su uso para medir agencia de autocuidado de la salud en población sana colombiana.

Introduction: The Revised Self-Care Agency Rating Scale is an instrument designed to assess health self-care in clinical population. However, studies on its psychometric properties in the general non-clinical Colombian population are unknown. Objective: To validate the Revised Self-Care Agency Rating Scale with a sample of 306 volunteer participants in Colombia. Method: Psychometric methodological study carried out between October and December 2021. For the analysis of the validity of the structure, factorial, exploratory and confirmatory analysis techniques were applied. In a complementary way, the content validity was evaluated through a panel of experts. Finally, as evidence of the instrument's reliability, its internal consistency was analyzed using McDonald's Omega coefficient. Results: The findings show that the ASA-R shows a one-dimensional structure that explains 61.99 % of the total variance. The assessment of the relevance, clarity, precision and comprehension of the items had results that had an average that ranged from an Aiken's V between 0.79 and 0.99. The internal consistency showed a satisfactory index for the total scale (ω=0.93). Conclusions: The version of the ASA-R presented in this article has adequate metric properties and its use is recommended to measure health self-care agency in a healthy Colombian population.

Introdução: a Revised Self-Care Agency Rating Scale é um instrumento desenvolvido para avaliar o autocuidado em saúde em uma população clínica; no entanto, estudos sobre suas propriedades psicométricas na população colombiana não clínica geral são desconhecidos. Objetivo: validar a Self-Care Agency Rating Scale-Revised em uma amostra de 306 participantes voluntários na Colômbia. Método: Estudo de desenho metodológico psicométrico realizado entre outubro e dezembro de 2021. Para a análise da validade da estrutura, foram aplicadas as técnicas de análise fatorial, exploratória e confirmatória; de forma complementar, a validade de conteúdo foi avaliada por meio de um painel de especialistas. Por fim, como evidência da confiabilidade do instrumento, sua consistência interna foi analisada por meio do coeficiente Ômega de McDonald's. Resultados: os achados mostram que o ASA-R apresenta uma estrutura unidimensional que explica 61,99 % da variância total. A avaliação da relevância, clareza, precisão e compreensão dos itens teve resultados cuja média variou de um V de Aiken entre 0,79 e 0,99. A consistência interna apresentou índice satisfatório para a escala total (ω=0,93). Conclusões: a versão do ASA-R apresentada neste artigo possui propriedades métricas adequadas e seu uso é recomendado para medir a agência de autocuidado em saúde em uma população colombiana saudável.

Porto-biliary fistula as an uncommon cause of haemobilia: A case report and literature review.

BACKGROUND: Haemobilia is a rare cause of gastrointestinal bleeding. It can be related to iatrogenic injuries, inflammatory diseases, and, more recently, postoperative, or post-procedure complications. Porto-biliary fistula is an uncommon case of haemobilia and has been related to iatrogenic injury or chronic inflammatory processes. To date, less than 30 cases of Porto-biliary fistula have been reported. CASE PRESENTATION: We present a 53 years-old woman with a history of biliary obstruction due to a choledochal cyst that required hepaticojejunostomy with evidence of anastomotic stricture. A percutaneous transhepatic biliary drainage (PTBD) was performed, with 3 failed attempts of percutaneous dilatation. A new hepaticojejunostomy was completed, however, 45 days later the patient presented to the emergency room with haemobilia and secondary hemodynamic instability. An emergency damage control laparotomy was performed, achieving bleeding control. In the second procedure, there is evidence of an ulcerative injury of the biliary tract secondary to a Porto-biliary fistula. CONCLUSION: Porto-biliary fistula is an entity that cannot be ruled out in cases of haemobilia, especially in cases with a history of bile duct surgical or percutaneous procedures. The prognosis is usually good if multidisciplinary management is performed, and the source of the bleeding is identified early.

A Novel, Quick, and Reliable Smartphone-Based Method for Serum PSA Quantification: Original Design of a Portable Microfluidic Immunosensor-Based System.

We describe a versatile, portable, and simple platform that includes a microfluidic electrochemical immunosensor for prostate-specific antigen (PSA) detection. It is based on the covalent immobilization of the anti-PSA monoclonal antibody on magnetic microbeads retained in the central channel of a microfluidic device. Image flow cytometry and scanning electron microscopy were used to characterize the magnetic microbeads. A direct sandwich immunoassay (with horseradish peroxidase-conjugated PSA antibody) served to quantify the cancer biomarker in serum samples. The enzymatic product was detected at -100 mV by amperometry on sputtered thin-film electrodes. Electrochemical reaction produced a current proportional to the PSA level, with a linear range from 10 pg mL-1 to 1500 pg mL-1. The sensitivity was demonstrated by a detection limit of 2 pg mL-1 and the reproducibility by a coefficient of variation of 6.16%. The clinical performance of this platform was tested in serum samples from patients with prostate cancer (PCa), observing high specificity and full correlation with gold standard determinations. In conclusion, this analytical platform is a promising tool for measuring PSA levels in patients with PCa, offering a high sensitivity and reduced variability. The small platform size and low cost of this quantitative methodology support its suitability for the fast and sensitive analysis of PSA and other circulating biomarkers in patients. Further research is warranted to verify these findings and explore its potential application at all healthcare levels.

Niveles de actividad física en población adulta del municipio Pereira, Colombia / Levels of physical activity in the adult population of the municipality of Pereira, Colombia

Introducción: En la calidad de vida tiene una fuerte influencia los estilos de vida. A su vez, la inactividad física es uno de los estilos de vida más perjudiciales que genera discapacidad y años de vida perdidos, debido a su papel en la génesis y exacerbación de las enfermedades crónicas no transmisibles. Objetivo: Determinar los niveles de actividad física y factores asociados en la población adulta del municipio Pereira. Métodos: Estudio transversal analítico. Se estimó una muestra de 1000 participantes entre las edades de 18 a 64 años. Los niveles de actividad física se midieron con el cuestionario internacional de actividad física versión larga. Se realizaron análisis bivariados. Resultados: El total de participantes fue de 996. La prevalencia global del cumplimiento de recomendaciones en actividad física del estudio fue de un 52,9 por ciento, en la que prevaleció el dominio de actividad física moderada. Los sujetos de mayor nivel socioeconómico obtuvieron menores prevalencias de actividad física. En cuanto a nivel de escolaridad, las categorías de tecnólogo (58 por ciento) y secundaria (57 por ciento) alcanzaron la más alta prevalencia de cumplimiento de recomendaciones mínimas. Conclusiones: Un poco más de la mitad de la población adulta pereirana cumple las recomendaciones mínimas de actividad física predominando la actividad de intensidad moderada, no obstante, la media presenta problemas de sobrepeso y obesidad. Es importante promover la práctica de actividad física de moderada a alta intensidad e involucrar a todos los estratos socioeconómicos(AU)

Introduction: Lifestyles have a strong influence on quality of life. In turn, physical inactivity is one of the most harmful lifestyles that generates disability and lost years of life, due to its role in the genesis and exacerbation of chronic non-communicable diseases. Objective: To determine the levels of physical activity and associated factors in the adult population of Pereira municipality. Methods: Analytical cross-sectional study. It was estimated a sample of 1000 participants in the ages from 18 to 64. Physical activity levels were measured with the long version of the international questionnaire of physical activity. Bivariate analyses were performed. Results: The total number of participants was 996. The overall prevalence of compliance with recommendations in physical activity of the study was 52.9 percent, in which the domain of moderate physical activity prevailed. Subjects of higher socio-economic status obtained lower prevalences of physical activity. In terms of schooling level, the categories of technologist (58 percent) and secondary school level (57 percent) reached the highest prevalence of compliance with minimum recommendations. Conclusions: A little more than half of the adult population of Pereira meets the minimum recommendations for physical activity, predominating moderate intensity activity; however, the average presents problems of overweight and obesity. It is important to promote the practice of moderate to high intensity physical activity and involve all socio-economic strata(AU)

Maxillary reconstruction with subperiosteal implants in a cancer patient: A one-year follow-up.

Introduction: To describe a clinical case on cancer patient with ablative tumor surgery, from treatment planning, surgical resection and subsequent implantological rehabilitation. Case Report: A 61-year-old male, diagnosed with a squamous cell carcinoma in the maxilla, requires the removal of the lesion and corresponding oral rehabilitation. However, two surgeries were necessary to rehabilitate the upper jaw. A custom-made prosthesis was fabricated. It was made from sintered titanium using machined subperiosteal implants with a universal external connection. Finally, a milled cobalt- chrome structure was produced and a feldspar ceramic covering was subsequently applied. Conclusions: Rehabilitation using subperiosteal implants may be an alternative tool for complex surgery involving large atrophies or cancer patients who have undergone highly ablative surgery. Key words:Oral rehabilitation, oral cancer, subperiostal implants.

Central sensitization in burning mouth syndrome: a practical approach using questionnaires.

OBJECTIVES: Some experts have suggested that burning mouth syndrome (BMS) should be included in the family of central sensitivity syndromes, a group of similar medical disorders linked by the central sensitization (CS) mechanism. Our objective is to assess the presence of CS in patients with BMS by performing a clinical examination and administering questionnaires to measure the generalized extent of pain, the presence of associated symptoms, and the number of other concurrent chronic pain conditions. STUDY DESIGN: We conducted a case-control study in 82 subjects (40 patients with BMS and 42 controls). Patients with BMS were diagnosed using The International Classification of Headache Disorders 3rd edition, beta version (ICHD-IIIß) criteria. The Widespread Pain Index (WPI) and Symptom Severity (SS) Score questionnaires were used to determine the degree of central sensitivity. The number of other concurrent chronic pain conditions was determined with the Neblett inventory. RESULTS: Data indicative of CS show a statistically significant association with BMS. Both SS Score and Widespread Pain Index scores higher in patients with BMS. Additionally, patients with BMS reported a significantly higher number of other central sensitivity syndromes. CONCLUSIONS: Patients with BMS could present a CS component as well as other chronic pain conditions. The use of questionnaires may be useful to determine the degree of central sensitivity in patients with BMS.

Closing gaps in histoplasmosis: clinical characteristics and factors associated with probable/histoplasmosis in HIV/AIDS hospitalized patients, a retrospective cross-sectional study in two tertiary centers in Pereira, Colombia.

BACKGROUND: The HIV pandemic continues to cause a high burden of morbidity and mortality due to delayed diagnosis. Histoplasmosis is prevalent in Latin America and Colombia, is difficult to diagnose and has a high mortality. Here we determined the clinical characteristics and risk factors of histoplasmosis in people living with HIV (PLWH) in Pereira, Colombia. MATERIALS AND METHODS: This was a retrospective cross-sectional study (2014-2019) involving two tertiary medical centers in Pereira, Colombia. People hospitalized with HIV were included. Histoplasma antigen detection was performed in urine samples. Probable histoplasmosis was defined according to European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group/National Institute of Allergy and Infectious Diseases Mycoses Study Group criteria. RESULTS: 172 HIV-infected patients were analyzed. Histoplasmosis was confirmed in 29% (n = 50/172) of patients. The logistic regression analysis showed that the risk factors for histoplasmosis were pancytopenia (OR 4.1, 95% CI 1.6-10.3, P = 0.002), < 50 CD4 + cells/µL (OR 3.1, 95% CI 1.3-7.3, P = 0.006) and Aspartate transaminase (AST) levels > 46 IU/L (OR 3.2, 95% CI 1.3-8, P = 0.010). CONCLUSIONS: Histoplasmosis is highly prevalent in hospitalized patients with HIV in Pereira, Colombia. The clinical findings are nonspecific, but there are some clinical abnormalities that can lead to suspicion of the disease, early diagnosis and prompt treatment. Urine antigen detection is useful for diagnosis, but is not widely available. An algorithmic approach is proposed for low-resource clinical settings.

Sociodemographic and academic characteristics of students in the Medical Program at the Universidad Tecnológica de Pereira. Cohorts 1977-2018 / Características sociodemográficas y académicas de los estudiantes del Programa de Medicina de la Universidad Tecnológica de Pereira. Cohortes 1977-2018

Abstract Problem situation: The characterization of students in a Medical Program and its impact on academic performance and successful completion is a relatively unstudied process in medical education. Material and Methods: This observational, descriptive, and cross-sectional study characterized students in the Medical Program at the Universidad Tecnológica de Pereira from 1977-2018. Results: Participants were grouped into applicants (23738), enrolled (3714), graduates (1984), and non-graduates (1730). In the enrolled student subgroup, the male:female ratio varied with a trend favoring females in terms of age when starting the program, age at graduation, graduation percentage, and periods enrolled. Application and enrollment were predominantly composed of students from lower socioeconomic strata, and who had graduated from public schools. There was a progressive participation of women in all groups, completing the program in less time and graduating at a younger age. The applicant/enrollment ratio was 25:1, unmet demand was 96% and the adjusted percentage of non-graduates was 27.4%. Conclusion: This information opens the discussion on the development of educational policies that seek to increase coverage and strengthen student follow-up programs, allowing for a timely and successful graduation.

Resumen Situación Problemática: La caracterización de los estudiantes de un Programa de Medicina y su impacto en el desempeño académico y egreso exitoso, es un proceso relativamente poco estudiado en la educación médica. Materiales y Métodos: El presente estudio observacional, descriptivo, de corte transversal, caracterizó los estudiantes del programa de Medicina de la Universidad Tecnológica de Pereira en el periodo 1977-2018. Resultados: Los participantes fueron clasificados en aspirantes (23738), matriculados (3714), graduados (1984) y no graduados (1730). En el subgrupo de estudiantes matriculados, la relación hombre: mujer fue variable con una tendencia que favoreció a la mujer en términos de edad de ingreso, edad de graduación, porcentaje de graduación y periodos matriculados. Predominó la inscripción y matrícula de estudiantes de estratos socioeconómicos bajos, provenientes del Eje Cafetero y de colegios púbicos. Se apreció una progresiva participación de las mujeres en todos los grupos que presentaron menor tiempo de duración de la carrera y menor edad en el momento de la graduación. La relación inscrita/matriculado fue de 25:1, la demanda insatisfecha fue del 96% y el porcentaje ajustado de estudiantes no graduados fue del 27.4%. Conclusión: Estos datos permiten la discusión sobre el desarrollo de políticas educativas que procuren un aumento en la cobertura y el fortalecimiento de programas de seguimiento del estudiante que le permitan un egreso oportuno y exitoso.

Dental implants rehabilitation in a patient with head and neck radiotherapy for osteosarcoma in the jaw. A clinical case report.

A 52-year-old female patient with a diagnostic of osteosarcoma in the mandible, in which it was necessary a reconstruction with a microvascularized osteomyocutaneous fibula bone. Coadjuvant chemotherapy was scheduled. Two years later, 4 osseointegrated implants (OII) were placed in the fibula a 2 OII in the right mandible, using a splint guided surgery. The final prosthodontic consisted in a metal ceramic restoration using CAD/ CAM technology. Key words:Oral rehabilitation, oral cancer, head and neck radiotherapy, oral oncology.

Jaw in a day: Osseointegration of the implants in the patient's leg before reconstructive surgery of a maxilla with ameloblastoma. A 4-year follow-up case report.

BACKGROUND: To describe a clinical case of a cancer patient who underwent ablative tumor surgery, including treatment planning, surgical resection and subsequent implant rehabilitation. CASE REPORT: A 61-year-old patient with a diagnosis of multicystic follicular ameloblastoma in the maxilla, in which it was necessary a multidisciplinary approach and two surgical steps. In the first surgical intervention osseointegrated implants (OII) were placed in the fibula, until their osseointegration period of 8 weeks was complete. Afterwards, in the second surgery, the micro-vascularized free fibular flap bearing the implants was transplanted into the oral cavity, in order to perform simultaneous reconstruction and early rehabilitation. The final prosthetic rehabilitation consisted in a hybrid prosthesis fabricated using CAD CAM technology. RESULTS: The latest advances in medical research have improved our understanding of the oral cavity's regenerative capacity after oncological treatment. This, aided by the advances in digital 3D technologies, has allowed meticulous treatment planning prior surgery. CONCLUSIONS: The functional and esthetic reconstructions described in these two case reports were made possible by coordinating multidisciplinary approaches involving dentists and oral and maxillofacial surgeons. Advances in medicine have improved understanding of the regenerative capacity of the oral region following oncologic treatment, facilitating meticulous advance planning, while advances in digital 3D technologies for planning make it possible to reduce the number of surgical sessions and the time taken for the patient to recover both the esthetics and function of the stomatognathic system. Key words:Oral rehabilitation, oral cancer, oral surgery.

Efectos de la fragilidad en los resultados adversos de la cirugía cardiaca en ancianos / Effects of frailty on the adverse outcomes of cardiac surgery in the elderly

Resumen Introducción: la fragilidad es una condición del paciente anciano, caracterizada por un estado de vulnerabilidad para desenlaces adversos en salud, incluidos los causados por la cirugía cardíaca. Objetivo: establecer la prevalencia de fragilidad en el contexto prequirúrgico del anciano sometido a cirugía cardiaca y establecer el riesgo de desenlaces adversos otorgado por esta condición. Metodología: estudio analítico de cohorte que valoró la fragilidad en 66 pacientes ancianos sometidos a cirugía cardiaca mediante el fenotipo físico de fragilidad de Fried. Se realizó un seguimiento a 30 días, durante el cual se estableció la prevalencia de fragilidad y el riesgo otorgado por esta condición a los desenlaces adversos de la cirugía cardiaca. Resultados: la edad de los pacientes osciló entre los 60 y los 83 años; la mediana fue de 70,5 años. La prevalencia de la fragilidad fue del 31,8%. La fragilidad incrementó los siguientes desenlaces adversos postoperatorios: muerte (p 0,001), ventilación prolongada (p 0,001), uso prolongado de vasoactivos (p 0,001) e infección del sitio operatorio (p 0,004). El riesgo de mortalidad otorgado por la fragilidad fue más alto que el de los puntajes usuales de valoración como el EuroSCORE II (HR 5,49; IC95% 1,0-28,5 vs. HR 1,17 IC95% 1,0-1,29). Conclusión: la fragilidad es una condición frecuente en el paciente anciano sometido a cirugía cardiaca e incrementa el riesgo de eventos adversos, en especial el de mortalidad. Se recomienda incluir la medición de la fragilidad en el protocolo prequirúgico del paciente anciano.

Abstract Introduction: Frailty is a common condition in the elderly patient, and is noted for its vulnerability for adverse outcomes in health, including those caused by cardiac surgery. Objective: To establish the prevalence of frailty in the pre-surgical context of the elderly subjected to cardiac surgery, as well as to establish the risk of adverse outcomes due to this condition. Method: An analytical cohort study was performed in order to evaluate frailty in 66 elderly patients subjected to cardiac surgery, using the physical phenotype of frailty described by Fried. A follow-up was made at 30 days, during which the frailty prevalence was established, as well the risk of adverse outcomes of the cardiac surgery due to this condition. Results: The age of the patients varied between 60 and 83 years, with a median of 70.5 years. The prevalence of frailty was 31.8%. Frailty increased the following post-surgical adverse outcomes: death (P = .001), longer ventilation time (P = .001), prolonged use of vasoactive drugs (P = .001), and infection of the surgical site (P = .004). The mortality risk due to frailty was higher than that of the usual evaluation scores such as Euro SCORE II (HR 5.49; 95% CI; 1.0-28.5 vs. HR 1.17: 95% CI; 1.0-1.29). Conclusion: Frailty is a common condition in the elderly patient subjected to cardiac surgery, and increases the risk of adverse events, particularly, mortality. It is recommended to use measurements of frailty in the pre-surgical protocol of the elderly patient.

Luminescent Lanthanide Metal Organic Frameworks as Chemosensing Platforms towards Agrochemicals and Cations.

Since the first studies of luminescent sensors based on metal organic frameworks (MOFs) about ten years ago, there has been an increased interest in the development of specific sensors towards cations, anions, explosives, small molecules, solvents, etc. However, the detection of toxic compounds related to agro-industry and nuclear activity is noticeably scarce or even non-existent. In this work, we report the synthesis and characterization of luminescent lanthanide-based MOFs (Ln-MOFs) with diverse crystalline architectures obtained by solvothermal methods. The luminescent properties of the lanthanides, and the hypersensitive transitions of Eu3+ (5D0→7F2) and Tb3+ (5D4→7F5) intrinsically found in the obtained MOFs in particular, were evaluated and employed as chemical sensors for agrochemical and cationic species. The limit of detection (LOD) of Tb-PSA MOFs (PSA = 2-phenylsuccinate) was 2.9 ppm for [UO22+] and 5.6 ppm for [Cu2+]. The variations of the 4f⁻4f spectral lines and the quenching/enhancement effects of the Ln-MOFs in the presence of the analytes were fully analyzed and discussed in terms of a combinatorial "host⁻guest" vibrational and "in-silico" interaction studies.

Factores de riesgo psicosocial de origen ocupacional, estrés y enfermedad coronaria / Psychosocial occupational risk factors, stress and coronary artery disease

Resumen Se hizo una revisión de 47 artículos derivados de investigaciones analíticas ubicados a través de buscadores y bases de datos electrónicas (ISI Web of Knowledge, APA Articles y Science Direct) con el propósito de identificar y analizar la información actualizada sobre condiciones del trabajo entendidas como factores de riesgo psicosocial de origen ocupacional y efectos sobre la salud cardiovascular, específicamente las denominadas cardiopatías coronarias. Se revisaron artículos en full text de estudios longitudinales con seguimiento de cohortes expuestas a los factores de riesgo, estudios de casos y controles y estudios cross sectional; se privilegiaron artículos de importancia en cuanto al tamaño muestral, los métodos usados, la representatividad de las muestras, la contundencia en sus resultados y haber sido publicados en revistas de alto impacto internacional. Se da cuenta de la discusión entre los dos grupos de investigación de mayor relevancia en el mundo: El IPD Consortium (Europa y Asia) y Unhealty Work (USA). El primer grupo reporta hallazgos de asociación entre los factores de riesgo psicosocial y enfermedad coronaria con una razón de disparidad (OD=1.8), y el UW reporta hallazgos con valores de 2.3 como fuerza de la asociación (OD=2.3). El debate sigue abierto, la investigación y la discusión avanzan cada día.

Abstract A review of articles derived from analytic investigations located through search engines and electronic databases was made (ISI Web of Knowledge, APA Articles and Science Direct) in order to identify and analyze up-to-date information on working conditions as psychosocial risk factors of occupational origin and effects on cardiovascular health, specifically the so-called coronary heart disease. Reviewed articles in full text from longitudinal studies with follow-up of cohorts exposed to the risk factors, cases and controls and cross sectional studies, are privileged items of importance in terms of sample size, the methods used, the representativeness of the samples, the forcefulness in their results and published in journals of high international impact. It reports on the argument between the two research groups of greatest relevance in the world: The IPD (Europe and Asia) Consortium and Unhealthy Work (USA). The first group reported findings of association between the risk factors of psychosocial and coronary heart disease with a ratio of average size (OD = 1. 8) gap, and the UW reports fiindings with values around 2.3 as strength of association (OD = 2 3). The debate remains open, the research and the discussion move forward every day.

Glut3 Addiction Is a Druggable Vulnerability for a Molecularly Defined Subpopulation of Glioblastoma.

While molecular subtypes of glioblastoma (GBM) are defined using gene expression and mutation profiles, we identify a unique subpopulation based on addiction to the high-affinity glucose transporter, Glut3. Although Glut3 is a known driver of a cancer stem cell phenotype, direct targeting is complicated by its expression in neurons. Using established GBM lines and patient-derived stem cells, we identify a subset of tumors within the "proneural" and "classical" subtypes that are addicted to aberrant signaling from integrin αvß3, which activates a PAK4-YAP/TAZ signaling axis to enhance Glut3 expression. This defined subpopulation of GBM is highly sensitive to agents that disrupt this pathway, including the integrin antagonist cilengitide, providing a targeted therapeutic strategy for this unique subset of GBM tumors.

Pobreza multidimensional y determinantes sociales de la salud. Línea de base para dos comunidades vulnerables / Multidimensional poverty index and social determinants of health. Baseline for two vulnerable communities

Resumen Introducción. Las políticas y programas del gobierno, orientados a disminuir las inequidades, tienen efectos sociales, económicos e impacto sobre las condiciones de salud; además, suelen carecer de procesos de evaluación que midan el impacto de sus intervenciones. Por lo tanto, este estudio plantea una línea de base del índice de pobreza multidimensional (IPM) en dos comunidades vulnerables, que permitirá evaluar, con posterioridad, el impacto del desarrollo en una zona franca. Materiales y métodos. El diseño del estudio fue observacional, descriptivo y transversal, a fin de establecer la línea de base de determinantes sociales de la salud, contenidos en el IPM y el efecto potencial de la intervención sobre las variables y dimensiones del IPM en dos corregimientos, antes del desarrollo de una zona franca. Resultados. Los hallazgos muestran las condiciones de vida precarias y de inequidad de las dos poblaciones, sobre todo en la dimensión educativa, los derechos de la población infantil y las condiciones de la vivienda. Las variables que mejor explican la pobreza son el analfabetismo, el rezago escolar y los pisos de tierra sobre los que es inadecuado habitar. La prevalencia de pobreza en Caimalito (80.9%) y Puerto Caldas (66.5%) supera la general de Colombia (24.8%) y de la región central (26.1%). Asimismo, la incidencia ajustada de pobreza en Caimalito (20.1%) y Puerto Caldas (18.5%) son mayores que la de Colombia (13%). Conclusión. Es necesario modificar variables del índice que no discriminen hogares pobres. La simulación de intervenciones aisladas tienen poco o ningún efecto en la reducción de la pobreza.

Abstract Introduction: Government policies and programs, aimed at reducing inequities, have a social and economic and impact on health conditions. However, they often lack evaluation processes that measure the impact of their interventions. Therefore, this study sets out a baseline of the Multidimensional Poverty Index (MPI) in two vulnerable communities, which will allow to assess the impact of development on a free zone. Materials and methods: Observational, descriptive and transversal study directed to establish the baseline of social determinants of health contained in the MPI, as well as the potential effect of the intervention on the variables and dimensions of the MPI in two precincts, before a free zone is developed. Results: Findings show the precarious living conditions and inequity in both populations, especially in the educational dimension, the rights of children and housing conditions. The variables that best explain poverty are illiteracy, education lag, and gravel floors which make habitability inappropriate. The prevalence of poverty in Caimalito (80.9%) and Puerto Caldas (66.5%) surpasses general poverty indexes in Colombia (24.8%) and in the central region (26.1%). Likewise, the adjusted incidence of poverty in Caimalito (20.1%) and Puerto Caldas (18.5%) is higher than in Colombia (13%). Conclusion: It is necessary to modify index variables that do not discriminate poor households. Simulation of isolated interventions have little or no effect on poverty reduction.

Cell-Free DNA from Ascites and Pleural Effusions: Molecular Insights into Genomic Aberrations and Disease Biology.

Collection of cell-free DNA (cfDNA) from the blood of individuals with cancer has permitted noninvasive tumor genome analysis. Detection and characterization of cfDNA in ascites and pleural effusions have not yet been reported. Herein, we analyzed cfDNA in the ascites and pleural effusions from six individuals with metastatic cancer. In all cases, cfDNA copy number variations (CNV) were discovered within the effusate. One individual had a relevant alteration with a high copy amplification in EGFR in a never smoker with lung cancer, who showed only MDM2 and CDK4 amplification in a prior tissue biopsy. Another subject with metastatic breast cancer had cytology-positive ascites and an activating PIK3CA mutation identified in the tissue, blood, and ascites collectively. This individual had tumor regression after the administration of the mTOR inhibitor everolimus and had evidence of chromotripsis from chromosomal rearrangements noted in the cell-free ascitic fluid. These results indicate that cfDNA from ascites and pleural effusions may provide additional information not detected with tumor and plasma cell-free DNA molecular characterization, and a context for important insights into tumor biology and clonal dynamic change within primary tumor and metastatic deposits. Mol Cancer Ther; 16(5); 948-55. ©2017 AACR.

El nuevo modelo de Atención Integral en Salud -MIAS- para Colombia. ¿La solución a los problemas del sistema / The new model of Integrated Health Care (IMAS) for Colombia: the solution to the system's problems?

El gobierno colombiano a través de la resolución 429 de 2016 (1), promulgó la política de Atención Integral en Salud, que pretende generar mejores condiciones de salud para la población a través de la regulación de la intervención sectorial e intersectorial, sustentado en lo dispuesto por un gran volumen de normatividad previa que comprende desde la ley 100 de 1993 y sus posteriores modificaciones incluyendo la ley 1438 de 2011 (2) que pretendía fortalecer y la Atención Primaria en Salud, el Plan decenal de Salud Pública, hasta la de más reciente expedición, la Ley estatutaria 1751, que luego de una gran controversia, consagró por fin la salud como un derecho fundamental. No obstante todos estos desarrollos en materia legislativa, no se han podido resolver los graves problemas por los que atraviesa el sector y que han sido reconocidos por el propio gobierno: fragmentación y desintegración de la atención; baja resolutividad; alta carga de enfermedad; fallos del mercado; incentivos negativos entre los agentes y falla regulatoria, lo que ha conllevado a aumentar la inequidad; corrupción; maltrato al usuario; desorden administrativo; modelo de prestación de servicios de salud morbicéntrico y centrado en los actores; deshumanizado; descontextualizado y sin prevalencia de derechos (2-3).

The Colombian government, through Resolution 429 of 2016 (1), enacted the Comprehensive Health Care policy, which aims to generate better health conditions for the population through the regulation of sectoral and intersectoral intervention, This policy is based on the provisions of a large volume of previous legislation ranging from Law 100 of 1993 and its subsequent amendments, including Law 1438 of 2011 (2), which sought to strengthen Primary Health Care and the Ten-Year Public Health Plan, to the more recent Statutory Law 1751, which, after much controversy, finally enshrined health as a fundamental right. Despite all these legislative developments, it has not been possible to resolve the serious problems facing the sector, which have been recognised by the government itself: fragmentation and disintegration of care; low resolvability; high disease burden; market failures; negative incentives among agents and regulatory failure, which has led to increased inequity; corruption; mistreatment of the user; administrative disorder; morbicentric and actor-centred health service delivery model; dehumanised; decontextualised and without prevalence of rights (2-3).