RESUMO
Six new dibenzo[b,e]oxepinone metabolites, chaetones A-F (1-6), as well as three known compounds, 1-hydroxy-6-methyl-8-hydroxymethylxanthone (7), citreorosein (8), and emodin (9), were obtained from a freshwater-derived fungal strain Chaetomium sp. YMF 1.02105. Their structures were established on the basis of extensive spectroscopic data analysis and comparison with spectroscopic data reported. Compounds 1-6 are further additions to the small group of dibenzo[b,e]oxepinones represented by arugosins A-H. Compounds 1-7 were tested for their cytotoxic activities against A549, Raji, HepG2, MCF-7, and HL-60 cell lines. The results showed that compound 3 had significant cytotoxicity with IC50 values of 1.2, 1.8, 1.9, 2.3, and 1.6 µg/mL, respectively, against the five cancer cell lines. All compounds showed modest antimicrobial activity against Staphylococcus aureus (ATCC 6538) in standard disk assays.
Assuntos
Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Chaetomium/química , Citotoxinas/isolamento & purificação , Citotoxinas/farmacologia , Antibacterianos/química , Linhagem Celular Tumoral/efeitos dos fármacos , Citotoxinas/química , Dibenzoxepinas/química , Dibenzoxepinas/isolamento & purificação , Dibenzoxepinas/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Água Doce/microbiologia , Humanos , Concentração Inibidora 50 , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Staphylococcus aureus/efeitos dos fármacosRESUMO
Three types with six new carboline alkaloids, trigonostemonines A-F (1- 6), were isolated from the roots and stems of Trigonostemon lii. Their structures were elucidated by spectroscopic analyses. It is the first time that beta-carboline alkaloids have been reported in Trigonostemon species. Trigonostemonine F (6) exhibited moderate cytotoxic activity against HL-60 with an IC (50) value of 16 microM.
Assuntos
Alcaloides/isolamento & purificação , Carbolinas/isolamento & purificação , Euphorbiaceae/química , Alcaloides/química , Carbolinas/química , Ensaios de Seleção de Medicamentos Antitumorais , Células HL-60 , Humanos , Análise Espectral/métodosRESUMO
Hostasinine A (1), a benzylphenethylamine alkaloid with an unprecedented skeleton featuring a C-4-C-6 linkage and a nitrone moiety, was isolated from Hosta plantaginea. Its structure was established on the basis of spectroscopic data, and was further confirmed by single-crystal X-ray diffraction. The alkaloid was postulated biogenetically from haemanthidine via N-oxidation and aza-aldol-type condensation and was synthesized biomimetically. The inhibitory activities of 1 on acetylcholinesterase (AChE) and two tumor cell lines (K562 and A549) were also evaluated.