MicroRNA-150-5p inhibits proliferation and invasion of osteosarcoma cells by down-regulating VEGFA.

OBJECTIVE: The aim of this study was to investigate whether microRNA-150-5p was involved in osteosarcoma cell proliferation and invasiveness via modulating vascular endothelial growth factor A (VEGFA) expression. PATIENTS AND METHODS: 10 pairs of osteosarcoma tissues and para-cancerous tissues were collected from patients with osteosarcoma in our center from February 2012 to July 2018. Relative expression levels of microRNA-150-5p and VEGFA in tissues and cell lines were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Luciferase reporter gene assay was conducted to illustrate the binding interplay between microRNA-150-5p and VEGFA. Furthermore, proliferative and invasive potentials in HOS and MG-63 cells regulated by both microRNA-150-5p and VEGFA were determined using Cell Counting Kit-8 (CCK-8), colony formation assay, and transwell assay, respectively. RESULTS: MicroRNA-150-5p was remarkably downregulated, while VEGFA was upregulated in osteosarcoma tissues compared with para-cancerous tissues (p<0.05). Similar results were observed in osteosarcoma cells and normal osteoblasts. Overexpression of microRNA-150-5p significantly inhibited the proliferation and invasion of osteosarcoma cells (p<0.05). Luciferase reporter gene assay demonstrated that microRNA-150-5p could target to VEGFA to negatively modulate its expression. In addition, the knockdown of VEGFA remarkably weakened osteosarcoma cell proliferative and invasive capacities (p<0.05). CONCLUSIONS: MicroRNA-150-5p weakens proliferative and invasive potentials in osteosarcoma cells by downregulating VEGFA level. All our findings suggest that microRNA-150-5p/VEGFA axis is a promising target for osteosarcoma treatment.

Risk factors for bowel resection and outcome in patients with incarcerated groin hernias.

PURPOSE: This retrospective study aimed to evaluate the risk factors for bowel resection and outcome in patients with incarcerated groin hernias. METHODS: The records of all adult patients who had undergone emergency hernia repair for incarcerated groin hernia from January 1999 to June 2009 were analyzed. One hundred and eighty-two patients with incarcerated groin hernias were included in this study. Bowel resection was required in 28 patients (15.4%). RESULTS: One hundred and twenty-six patients (69.2%) were covered by various types of health insurance. Twenty-six patients (14.3%) were hospitalized for mechanical bowel obstruction or had obvious symptoms of simultaneous bowel obstruction. Obvious peritonitis was found in seven patients (3.8%). A logistic regression model identified three independent risk factors for bowel resection: lack of health insurance (odds ratio [OR], 5, P = 0.005), obvious peritonitis (OR, 11.52, P = 0.019), and femoral hernia (OR, 8.31, P < 0.001). Postoperative complications (standardized coefficient [SC] = 0.478, P < 0.001), presentation of bowel obstruction on admission (SC = 0.169, P = 0.017), and having health insurance (SC = 0.153, P = 0.030) were associated with prolonged length of stay. No factors were found to be directly associated with morbidity or mortality. CONCLUSIONS: Risk for intestinal resection in emergency groin hernia repair is higher in patients with femoral hernia, obvious peritonitis, or those with no health insurance. Surgeons should pay more attention to these patients and initiate emergency surgery without delay.