RESUMO
Potent stem/progenitor cells have been isolated from normal human dental pulps, termed dental pulp stem cells (DPSCs). However, no study has described the presence of stem cell populations in human dental pulp from the third molar with embryonic phenotypes. The dental pulp tissue was cultured in media with the presence of LIF, EGF, and PDGF. In the present study, we describe a new population of pluripotent stem cells that were isolated from dental pulp (DPPSC). These cells are SSEA-4(+), Oct4(+), Nanog(+), FLK-1(+), HNF3beta(+), Nestin(+), Sox2(+), Lin28(+), c-Myc(+), CD13(+), CD105(+), CD3(-), CD45(-), CD90(low), CD29(+), CD73(low), STRO-1(low) and CD146(-). We have investigated by SEM analysis and q-RT-PCR the capacity of DPPSCs to 3D differentiate in vitro using the Cell Carrier 3D glass scaffold into tissues that have similar characteristics to embryonic mesoderm and endoderm layers. These data would support the use of these cells, which are derived from an easily accessible source and can be used in future regeneration protocols for many tissue types that differentiate from the three embryonic layers.
Assuntos
Polpa Dentária/citologia , Células-Tronco Pluripotentes Induzidas/citologia , Dente Serotino , Adolescente , Adulto , Biomarcadores/metabolismo , Diferenciação Celular , Células Cultivadas , Polpa Dentária/metabolismo , Feminino , Humanos , Células-Tronco Pluripotentes Induzidas/metabolismo , Células-Tronco Pluripotentes Induzidas/ultraestrutura , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/ultraestrutura , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Alicerces Teciduais , Adulto JovemAssuntos
Laparoscopia , Doença Mista do Tecido Conjuntivo/complicações , Esplenectomia , Trombocitopenia/etiologia , Adulto , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/uso terapêutico , Feminino , Seguimentos , Humanos , Contagem de Plaquetas , Cuidados Pós-Operatórios , Prednisona/administração & dosagem , Prednisona/uso terapêutico , Trombocitopenia/cirurgia , Trombocitopenia/terapia , Fatores de TempoRESUMO
We present the case of a 33 year old man who consulted because a lumbago; a bone lesion was detected on a lumbosacral radiography, and finally was diagnosed of Multiple Myeloma. The illness, diagnosis and epidemiology are briefly analyzed, rebounding the rarity of its presentation in a young man.