RESUMO
Protontherapy is hadrontherapy's fastest-growing modality and a pillar in the battle against cancer. Hadrontherapy's superiority lies in its inverted depth-dose profile, hence tumour-confined irradiation. Protons, however, lack distinct radiobiological advantages over photons or electrons. Higher LET (Linear Energy Transfer) 12C-ions can overcome cancer radioresistance: DNA lesion complexity increases with LET, resulting in efficient cell killing, i.e. higher Relative Biological Effectiveness (RBE). However, economic and radiobiological issues hamper 12C-ion clinical amenability. Thus, enhancing proton RBE is desirable. To this end, we exploited the p + 11B â 3α reaction to generate high-LET alpha particles with a clinical proton beam. To maximize the reaction rate, we used sodium borocaptate (BSH) with natural boron content. Boron-Neutron Capture Therapy (BNCT) uses 10B-enriched BSH for neutron irradiation-triggered alpha particles. We recorded significantly increased cellular lethality and chromosome aberration complexity. A strategy combining protontherapy's ballistic precision with the higher RBE promised by BNCT and 12C-ion therapy is thus demonstrated.
Assuntos
Terapia por Captura de Nêutron de Boro/métodos , Boro/uso terapêutico , Terapia Combinada/métodos , Nêutrons , Neoplasias da Próstata/radioterapia , Terapia com Prótons , Terapia com Prótons/métodos , Partículas alfa/uso terapêutico , Animais , Boroidretos/química , Boro/química , Terapia por Captura de Nêutron de Boro/instrumentação , Isótopos de Carbono/química , Morte Celular/efeitos da radiação , Linhagem Celular Tumoral , Aberrações Cromossômicas/efeitos da radiação , Terapia Combinada/instrumentação , Ciclotrons , Dano ao DNA , DNA de Neoplasias/genética , DNA de Neoplasias/metabolismo , DNA de Neoplasias/efeitos da radiação , Relação Dose-Resposta à Radiação , Corantes Fluorescentes/química , Humanos , Cariotipagem , Transferência Linear de Energia , Masculino , Neoplasias da Próstata/patologia , Terapia com Prótons/instrumentação , Eficiência Biológica Relativa , Compostos de Sulfidrila/químicaRESUMO
Stüve-Wiedemann syndrome (SWS) is an extremely rare congenital skeletal disorder associated with significant newborn mortality and morbidity in survivors. Prenatal diagnosis is reportedly possible, but a precise diagnosis is difficult because SWS is part of a heterogeneous group of bone dysplasias. Molecular analysis remains the gold standard for establishing a specific diagnosis of this kind of disorders and for providing effective prenatal counselling. This article presents a case of SWS suspected at prenatal ultrasound in the second trimester of pregnancy and confirmed by multidisciplinary approach at birth.
Assuntos
Doenças do Desenvolvimento Ósseo/congênito , Doenças do Desenvolvimento Ósseo/diagnóstico por imagem , Ultrassonografia Pré-Natal , Adulto , Feminino , Humanos , Recém-Nascido , Gravidez , SíndromeAssuntos
Hidrocarbonetos Clorados/intoxicação , Acetilcisteína/uso terapêutico , Doença Aguda , Adolescente , Adulto , Idoso , Antídotos/uso terapêutico , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Prognóstico , Propano/análogos & derivados , Propano/intoxicação , Estudos Retrospectivos , Tricloroetileno/intoxicaçãoRESUMO
The Onley-Yip procedure for determining ethylenethiourea (ETU) in milk and crops was modified to reduce interferences by the ethylenebisdithiocarbamates (EBDCs). A 20 g crop-methanol extract is cleaned up by adsorbing the sample onto Gas-Chrom S. desorbing ETU, and eluting ETU from aluminum oxide with chloroform containing ethanol. ETU is converted to the S-butyl derivative for gas-liquid chromatography (GLC) and flame photometric detection (sulfur mode). For liquid chromatography (LC), ETU is cleaned up on another aluminum oxide column and injected directly. LC and GLC results are confirmed by thin layer chromatography. A cooking procedure based on conversion of EBDCs to ETU is included for surveying crops for possible EBDC content. Recoveries from 8 crops and milk fortified at 0.05 ppm ETU ranged from 73 to 100%.