Assuntos
Caspase 3/genética , Regulação Neoplásica da Expressão Gênica/genética , Expressão Gênica/genética , Poli(ADP-Ribose) Polimerases/genética , Neoplasias da Próstata/genética , Regulação para Cima/genética , Caspase 3/metabolismo , Linhagem Celular Tumoral , Humanos , Masculino , Poli(ADP-Ribose) Polimerase-1 , Poli(ADP-Ribose) Polimerases/metabolismoRESUMO
AIM: To determine whether normal human amnion-derived mesenchymal stromal cells (hAMSCs) secrete trophic mediators able to inhibit human prostate cancer cell lines growth. METHODS: Human prostate cancer and normal cell lines were used. Mesenchymal stromal cells (MSC) were isolated through mechanical and enzymatic digestion from amniotic membranes and were evaluated for specific mesenchymal stromal cells antigens. Cell proliferation was examined by MTT assay. Staining with propidium iodide (PI) followed by flow cytometry was used to detect cell cycle phase. RESULTS: hAMSC showed proper mesenchymal stem cells phenotype. We found that hAMSC conditioned media (CM) inhibited prostate cancer cells proliferation. Indeed, we demonstrated that hAMSC CM treatment increased percentage of G1 cancer cells and decreased percentage of cancer cells in S and G2M phases, suggesting that the hAMSC factors slow progression of prostate cancer cells through cell cycle inhibition. CONCLUSIONS: Our study provide evidences that hAMSC microenvironment secretes soluble factors able to inhibit prostate cancer cells growth. This may represent a novel strategy to control proliferation of prostate cancer through modulation of the host microenvironment.
Assuntos
Proliferação de Células/efeitos dos fármacos , Células-Tronco Mesenquimais/fisiologia , Próstata/efeitos dos fármacos , Neoplasias da Próstata/tratamento farmacológico , Âmnio/citologia , Técnicas de Cultura de Células , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Meios de Cultivo Condicionados/farmacologia , Humanos , Masculino , Neoplasias da Próstata/patologiaAssuntos
Carcinoma de Células Escamosas/genética , Caspase 3/fisiologia , Expressão Gênica/fisiologia , Neoplasias de Cabeça e Pescoço/genética , Doença de Parkinson/genética , Poli(ADP-Ribose) Polimerases/fisiologia , Apoptose/genética , Carcinoma de Células Escamosas/complicações , Neoplasias de Cabeça e Pescoço/complicações , Humanos , Doença de Parkinson/complicações , Poli(ADP-Ribose) Polimerase-1 , Reação em Cadeia da Polimerase em Tempo RealRESUMO
INTRODUCTION: Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world. Risk factors for this cancer include tobacco and alcohol use, ultraviolet light exposure, and viral infection. Parkinson's disease is one of the most common neurodegenerative disorders, with a prevalence of 3% in persons over the age of 65 years. Apoptosis is a programmed cell death machinery pivotal for normal development, the establishment of highly organized neuronal circuitry, and the elimination of cancer cells. It has been suggested that increased expression of proapoptotic genes is associated with head tumors. One of these genes is the leucine zipper, down-regulated in cancer 1 (LDOC1) gene. CASE REPORT: We report two interesting cases of a 79-year-old man and a 98-year-old woman, both with Parkinson's disease and well-differentiated multiple HNSCC, in whom we evaluated the possible differential expression of LDOC1. RESULTS: We found that LDOC1 gene expression was increased in both patients compared with three male and three female controls. Conclusions. These findings suggest that apoptosis may play a pathogenetic role in HNSCC.
Assuntos
Apoptose , Carcinoma Basocelular/química , Carcinoma de Células Escamosas/química , Neoplasias de Cabeça e Pescoço/química , Leucócitos/química , Proteínas Nucleares/análise , Doença de Parkinson/complicações , Proteínas Supressoras de Tumor/análise , Idoso , Idoso de 80 Anos ou mais , Antiparkinsonianos/uso terapêutico , Carcinoma Basocelular/sangue , Carcinoma Basocelular/complicações , Carcinoma Basocelular/patologia , Carcinoma de Células Escamosas/sangue , Carcinoma de Células Escamosas/complicações , Carcinoma de Células Escamosas/patologia , Eletroforese em Gel de Ágar , Neoplasias Faciais/química , Feminino , Regulação Neoplásica da Expressão Gênica , Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/metabolismo , Neoplasias de Cabeça e Pescoço/sangue , Neoplasias de Cabeça e Pescoço/complicações , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Zíper de Leucina , Levodopa/uso terapêutico , Masculino , Proteínas Nucleares/genética , Doença de Parkinson/tratamento farmacológico , RNA Mensageiro/análise , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Espectrofotometria , Neoplasias da Língua/química , Neoplasias da Língua/metabolismo , Proteínas Supressoras de Tumor/genéticaRESUMO
We report the case of a 74-year-old man with Alzheimer's disease (AD) and an extensive ulcerative lesion on the right ear. AD is a neurodegenerative disease with progressive loss of memory and cognitive deterioration. It has been suggested that apoptotic cell injury and eventually cell death is a major contributor to the AD neurodegenerative process. The ulcerative lesion was surgically excised and the histological analysis reported a well-differentiated squamous cell carcinoma. Caspase-3 (CASP3) plays an important role in neuronal death during nervous system development and under certain pathological conditions. Furthermore, in vitro and in vivo studies reported elevated expression and activation of CASP3 in models of AD. Molecular epidemiological studies suggest that CASP3 may contribute to head and neck squamous cell carcinoma susceptibility and disease progression and that increased CASP3 expression is associated with tumors of the head. Also poly (ADP-ribose) polymerase 1 (PARP1) and the leucine zipper downregulated in cancer 1 (LDOC1) genes play a proapoptotic role. We therefore evaluated the differential expression of LDOC1, PARP1, and CASP3 mRNA in peripheral blood leukocytes of our patient. We found increased expression of all these genes compared with the expression in control subjects.