The Effects of Caffeine on Heart Rate and Heart Rate Variability at Rest and During Submaximal Cycling Exercise.

The aim of this study was to investigate the effects of caffeine on heart rate and heart rate variability (HRV) at rest and during submaximal exercise. Using a balanced, double-blind, randomized, crossover design, 16 male cyclists (age: 37 ± 9 years; V˙O2max: 4.44 ± 0.67 L·min-1) completed three trials in an air-conditioned laboratory. In Trial 1, cyclists completed two incremental cycling tests to establish the V˙O2-power output relationship and V˙O2max. In trials 2 and 3, cyclists were evaluated for heart rate and HRV at rest, after which they ingested a capsule containing 5 mg·kg-1 of caffeine or placebo. Thirty-five minutes post-supplementation, additional resting heart rate and HRV readings were taken after which cyclists completed a submaximal incremental cycling test (6 min stages) at 40-80% of V˙O2max; with HR and HRV measurements taken in the last 5 min of each increment. HRV was determined from the root mean square of successive differences between R-R intervals. There were significant supplement × exercise intensity interactions on heart rate (p = .019) and HRV (p = .023), with post hoc tests on the latter showing that caffeine increased HRV at 40%, 50%, and 60% of V˙O2max by 3.6 ± 4.9, 2.6 ± 2.8, and 0.6 ± 1.7 ms, respectively. There was a supplement × time interaction effect on resting HRV (p < .001), but not on heart rate (p = .351). The results of this study support the suggestion that caffeine increases the parasympathetic modulation of heart rate.Clinical trial registration number: NCT05521386.

The efficacy of genotype-based dietary or physical activity advice in changing behavior to reduce the risk of cardiovascular disease, type II diabetes mellitus or obesity: a systematic review and meta-analysis.

CONTEXT: Despite clear evidence that adherence to dietary and physical activity advice can reduce the risk of cardiometabolic disease, a significant proportion of the population do not follow recommendations. Personalized advice based on genetic variation has been proposed for motivating behavior change, although research on its benefits to date has been contradictory. OBJECTIVE: To evaluate the efficacy of genotype-based dietary or physical activity advice in changing behavior in the general population and in individuals who are at risk of cardiovascular disease (CVD) or type II diabetes mellitus (T2DM). DATA SOURCES: MEDLINE, EMBASE, PsycInfo, and the Cochrane Central Register of Controlled Trials (CENTRAL) were searched up to January 7, 2022. Randomized controlled trials of a genotype-based dietary and/or physical activity advice intervention that aimed to change dietary and/or physical activity behavior were included. DATA EXTRACTION: Abstracts of 7899 records were screened, and 14 reports from 11 studies met the inclusion criteria. DATA ANALYSIS: Genotype-based dietary or physical activity advice was found to have no effect on dietary behavior in any of the studies (standardized mean difference [SMD] .00 [-.11 to .11], P = .98), even when analyzed by subgroup: "at risk" (SMD .00 [-.16 to .16, P = .99]; general population (SMD .01 [-.14 to .16], P = .87). The physical activity behavior findings were similar for all studies (SMD -.01 [-.10 to .08], P = .88), even when analyzed by subgroup: "at risk" (SMD .07 [-.18 to .31], P = .59); general population (SMD -.02 [-.13 to .10], P = .77). The quality of the evidence for the dietary behavior outcome was low; for the physical activity behavior outcome it was moderate. CONCLUSIONS: Genotype-based advice does not affect dietary or physical activity behavior more than general advice or advice based on lifestyle or phenotypic measures. This was consistent in studies that recruited participants from the general population as well as in studies that had recruited participants from populations at risk of CVD or T2DM. SYSTEMATIC REVIEW REGISTRATION: PROSPERO registration no. CRD42021231147.

Caffeine, exercise physiology, and time-trial performance: no effect of ADORA2A or CYP1A2 genotypes.

The aim of this study was to investigate the influence of ADORA2A and CYP1A2 genotypes on the physiological and ergogenic effects of caffeine. Sixty-six male cyclists were screened for ADORA2A and CYP1A2 genotypes; with 40 taking part subsequently in a randomised, double-blind, placebo-controlled study. Trial 1 was used to establish the oxygen uptake-power output relationship and maximal oxygen uptake. In trials 2 and 3, participants ingested 5 mg·kg-1 of caffeine or placebo 1 h before completing a submaximal incremental cycling test, followed by a time-trial (∼30 min). Relative to placebo, caffeine led to a significant reduction in time to complete the time-trial (caffeine: 29.7 ± 1.8 min; placebo: 30.8 ± 2.3 min); but there was no effect of genotype. During submaximal exercise, caffeine reduced mean heart rate by 2.9 ± 3.7 beats·min-1, with effects dissipating as exercise intensity increased. Caffeine also significantly reduced perceived exertion by 0.5 ± 0.8, and increased blood lactate by 0.29 ± 0.42 mmol·L-1, respiratory exchange ratio by 0.013 ± 0.032, and minute ventilation by 3.1 ± 6.8 L·min-1. Nonetheless, there were no supplement × genotype interactions. In conclusion, caffeine influences physiological responses to submaximal exercise and improves time-trial performance irrespective of ADORA2A or CYP1A2 genotypes. Novelty: Caffeine affects physiological responses at rest and during submaximal exercise independent of ADORA2A or CYP1A2 genotypes. Variability in the effect of caffeine on time-trial performance is not explained by ADORA2A or CYP1A2 genotypes.

The effect of the menstrual cycle on running economy.

BACKGROUND: The aim of this study was to examine the effect of the menstrual cycle on running economy (RE). METHODS: Using a repeated-measures design, ten eumenorrheic, trained female runners (age: 32±6 yrs, V̇O2max: 59.7±4.7 mL·kg-1·min-1) completed four, weekly, identical sub-maximal and maximal incremental step tests on a treadmill to measure physiological responses across a full menstrual cycle. For phase comparison, the results from the trials that fell in the early follicular (low estrogen, low progesterone), late follicular (high estrogen, low progesterone) and mid-luteal (high estrogen, high progesterone) phases were used. RESULTS: There was a significant effect of menstrual cycle phase on RE (P=0.001), with RE in the mid-luteal (ML) phase being worse than that of the early follicular (EF) (+2.33 mL·kg-1·min-1; P=0.026) and late follicular (LF) (+2.17 mL·kg-1·min-1; P=0.011) phases. The ML phase also resulted in elevated core temperature versus the EF (+0.51 ºC; P=0.001) and LF (+0.66 ºC; P=0.037) phases, and elevated minute ventilation versus the EF phase (+3.83 L·min-1; P=0.003). No significant effects of menstrual cycle phase were found on body mass, heart rate, ratings of perceived exertion, time-to-exhaustion, maximal oxygen consumption, or blood lactate concentration. CONCLUSIONS: In the ML phase, which causes increased core temperature and minute ventilation, RE is impaired at exercise intensities that are applicable to training and performance. In physiologically stressful environments, this impairment in RE may have a significant impact on training and performance.

The Effects of Sodium Phosphate Supplementation on Physiological Responses to Submaximal Exercise and 20 km Cycling Time-Trial Performance.

The aim of this study was to examine the effects of sodium phosphate (SP) supplementation on physiological responses to submaximal exercise and 20 km cycling time-trial performance. Using a randomized, double-blind, crossover design, 20 endurance-trained male cyclists (age: 31 ± 6 years; height: 1.82 ± 0.07 m; body mass: 76.3 ± 7.0 kg; maximal oxygen uptake [V̇O2max]: 57.9 ± 5.5 mL·kg-1·min-1) completed two supplementation trials separated by a 14-day washout period. The trials consisted of 10 minutes of cycling at 65% V̇O2max followed by a 20 km time trial. Expired air was monitored throughout each trial for the evaluation of V̇O2, minute ventilation (V̇E), and respiratory exchange ratio (RER). Heart rate was monitored during each trial along with ratings of perceived exertion (RPE) and blood lactate concentration. For four days before each trial, participants ingested 50 mg·kg fat-free mass-1·day-1 of either SP or placebo. There were no effects (p ≥ .05) of supplementation on physiological responses during cycling at 65% V̇O2max. There were also no effects of supplementation on time-trial performance (placebo: 32.8 ± 2.2 min; SP: 32.8 ± 2.3 min). Nevertheless, relative to placebo, SP increased V̇E (mean difference: 3.81 L·min-1; 95% confidence interval: [0.16, 7.46 L·min-1]), RER (mean difference: 0.020; 95% confidence interval: [0.004, 0.036]), and RPE (mean difference: 0.39; 95% confidence interval: [0.04, 0.73]) during time trials, as well as post time-trial blood lactate concentration (mean difference: 1.06 mmol·L-1; 95% confidence interval: [0.31, 1.80 mmol·L-1]). In conclusion, SP supplementation has no significant effects on submaximal physiological responses or 20 km time-trial performance.

Moderate-Intensity Oxygen Uptake Kinetics: Is a Mono-Exponential Function Always Appropriate to Model the Response?

PURPOSE: This study investigated the existence of the oxygen uptake ([Formula: see text]) overshoot and the effects of exercise intensity and fitness status on the [Formula: see text] response during moderate-intensity exercise. METHODS: Twelve "high-fitness" (Mage = 26 ± 5 years; Mheight = 184.1 ± 5.4 cm; Mbody mass = 76.6 ± 8.9 kg; mean peak oxygen uptake ([Formula: see text]peak) = 59.0 ± 3.3 mL·kg-1·min·-1) and 11 "moderate-fitness" (Mage = 29 ± 5 years; Mheight = 178.7 ± 7.5 cm; Mbody mass = 81.7 ± 10.9 kg; MV̇O2peak = 45.2 ± 3.1 mL·kg-1·min·-1) participants performed square-wave transitions from unloaded cycling to 3 different intensities (70%, 82.5%, and 95% of the gas exchange threshold). The data were modeled using both a mono-exponential function (Model 1) and a function that included a switch-on component (Model 2). The overshoot was computed by subtracting the steady state from the peak of the modeled response and by calculating the area of the curve that was above steady state. RESULTS: The goodness of fit was affected by model type (p = .002) and exercise intensity (p < .001). High-fitness participants displayed a smaller τ (p < .05) and a larger amplitude (p < .05) and were more likely to overshoot the steady state (p = .035). However, while exercise intensity did affect the amplitude (p < .001), it did not affect τ (p ≥ .05) or the likelihood of an overshoot occurring (p = .389). CONCLUSION: While exercise intensity did not alter the [Formula: see text] response, fitness status affected τ and the likelihood of an overshoot occurring. The overshoot questions the traditional approach to modeling moderate-intensity [Formula: see text] data.

Caffeine and Physiological Responses to Submaximal Exercise: A Meta-Analysis.

The aim of this study was to carry out a systematic review and meta-analysis of the effects of caffeine supplementation on physiological responses to submaximal exercise. A total of 26 studies met the inclusion criteria of adopting double-blind, randomized crossover designs that included a sustained (5-30 min) fixed-intensity bout of submaximal exercise (constrained to 60-85% maximal rate of oxygen consumption) using a standard caffeine dose of 3-6 mg·kg-1 administered 30-90 min prior to exercise. Meta-analyses were completed using a random-effects model, and data are presented as raw mean difference (D) with associated 95% confidence limits (CLs). Relative to placebo, caffeine led to significant increases in submaximal measures of minute ventilation (D = 3.36 L·min-1; 95% CL, 1.63-5.08; P = .0001; n = 73), blood lactate (D = 0.69 mmol·L-1; 95% CL, 0.46-0.93; P < .00001; n = 208), and blood glucose (D = 0.42 mmol·L-1; 95% CL, 0.29-0.55; P < .00001; n = 129). In contrast, caffeine had a suppressive effect on ratings of perceived exertion (D = -0.8; 95% CL, -1.1 to -0.6; P < .00001; n = 147). Caffeine had no effect on measures of heart rate (P = .99; n = 207), respiratory exchange ratio (P = .18; n = 181), or oxygen consumption (P = .92; n = 203). The positive effects of caffeine supplementation on sustained high-intensity exercise performance are widely accepted, although the mechanisms to explain that response are currently unresolved. This meta-analysis has revealed clear effects of caffeine on various physiological responses during submaximal exercise, which may help explain its ergogenic action.

A comparison of methods to estimate anaerobic capacity: Accumulated oxygen deficit and W' during constant and all-out work-rate profiles.

This study investigated (i) whether the accumulated oxygen deficit (AOD) and curvature constant of the power-duration relationship (W') are different during constant work-rate to exhaustion (CWR) and 3-min all-out (3MT) tests and (ii) the relationship between AOD and W' during CWR and 3MT. Twenty-one male cyclists (age: 40 ± 6 years; maximal oxygen uptake [V̇O2max]: 58 ± 7 ml · kg-1 · min-1) completed preliminary tests to determine the V̇O2-power output relationship and V̇O2max. Subsequently, AOD and W' were determined as the difference between oxygen demand and oxygen uptake and work completed above critical power, respectively, in CWR and 3MT. There were no differences between tests for duration, work, or average power output (P ≥ 0.05). AOD was greater in the CWR test (4.18 ± 0.95 vs. 3.68 ± 0.98 L; P = 0.004), whereas W' was greater in 3MT (9.55 ± 4.00 vs. 11.37 ± 3.84 kJ; P = 0.010). AOD and W' were significantly correlated in both CWR (P < 0.001, r = 0.654) and 3MT (P < 0.001, r = 0.654). In conclusion, despite positive correlations between AOD and W' in CWR and 3MT, between-test differences in the magnitude of AOD and W', suggest that both measures have different underpinning mechanisms.

Multiple sprint work : physiological responses, mechanisms of fatigue and the influence of aerobic fitness.

The activity patterns of many sports (e.g. badminton, basketball, soccer and squash) are intermittent in nature, consisting of repeated bouts of brief (