Seaweeds as Nutraceutical Elements and Drugs for Diabetes Mellitus: Future Perspectives.

Diabetes mellitus is a chronic metabolic condition marked by high blood glucose levels caused by inadequate insulin synthesis or poor insulin use. This condition affects millions of individuals worldwide and is linked to a variety of consequences, including cardiovascular disease, neuropathy, nephropathy, and retinopathy. Diabetes therapy now focuses on controlling blood glucose levels through lifestyle changes, oral medicines, and insulin injections. However, these therapies have limits and may not successfully prevent or treat diabetic problems. Several marine-derived chemicals have previously demonstrated promising findings as possible antidiabetic medicines in preclinical investigations. Peptides, polyphenols, and polysaccharides extracted from seaweeds, sponges, and other marine species are among them. As a result, marine natural products have the potential to be a rich source of innovative multitargeted medications for diabetes prevention and treatment, as well as associated complications. Future research should focus on the chemical variety of marine creatures as well as the mechanisms of action of marine-derived chemicals in order to find new antidiabetic medicines and maximize their therapeutic potential. Based on preclinical investigations, this review focuses on the next step for seaweed applications as potential multitargeted medicines for diabetes, highlighting the bioactivities of seaweeds in the prevention and treatment of this illness.

From Ocean to Medicine: Harnessing Seaweed's Potential for Drug Development.

Seaweed, a miscellaneous group of marine algae, has long been recognized for its rich nutritional composition and bioactive compounds, being considered nutraceutical ingredient. This revision delves into the promising role of seaweed-derived nutrients as a beneficial resource for drug discovery and innovative product development. Seaweeds are abundant sources of essential vitamins, minerals, polysaccharides, polyphenols, and unique secondary metabolites, which reveal a wide range of biological activities. These bioactive compounds possess potential therapeutic properties, making them intriguing candidates for drug leads in various medical applications and pharmaceutical drug development. It explores their pharmacological properties, including antioxidant, anti-inflammatory, antimicrobial, and anticancer activities, shedding light on their potential as therapeutic agents. Moreover, the manuscript provides insights into the development of formulation strategies and delivery systems to enhance the bioavailability and stability of seaweed-derived compounds. The manuscript also discusses the challenges and opportunities associated with the integration of seaweed-based nutrients into the pharmaceutical and nutraceutical industries. Regulatory considerations, sustainability, and scalability of sustainable seaweed sourcing and cultivation methods are addressed, emphasizing the need for a holistic approach in harnessing seaweed's potential. This revision underscores the immense potential of seaweed-derived compounds as a valuable reservoir for drug leads and product development. By bridging the gap between marine biology, pharmacology, and product formulation, this research contributes to the critical advancement of sustainable and innovative solutions in the pharmaceutical and nutraceutical sectors.

Toxicity of two pesticides in binary mixture on survival and enzymatic response of Cerastoderma edule - The warming influence.

Pesticide application increased by about 1 million tonnes in the last 3 decades. Pesticides' overuse, coupled with the need for several pesticides to control different pests in the same crop, and its application many times per year, results in dangerous chemical cocktails that enter in aquatic systems, with impacts to the ecosystems and its communities. Climatic changes are currently another great concern, is predicted by the end of the 21st century, the earth's surface temperature will increase by about 4 °C. Bivalve species are reported as essential to the ecosystems' balance. However, they are also indicated as the organisms that will suffer the most serious effects of the temperature increase. So, this work intends to: a) verify the harm of the sub-lethal concentrations of two worldwide used pesticides, oxyfluorfen and copper (Cu), when combined, to Cerastoderma edule at 15 °C and 20 °C; b) assess the changes in the antioxidant defence system, the activity of the neurological enzyme acetylcholinesterase and the nutritive value of C. edule, after exposure to sub-lethal concentrations of oxyfluorfen and Cu, single and in the mixture, at 15 °C and 20 °C; c) observe the interaction between Cu and oxyfluorfen, considering the different biomarkers. Bivalves were exposed to oxyfluorfen and Cu, single and combined, for 96 h, at 15 °C and 20 °C. Results showed lethal effects to the organisms exposed at 20 °C when exposed to the highest binary mixture concentrations. Biochemical effects were observed on the organisms exposed to 15 °C, despite not observing any lethal effects. Briefly, there was a reported increase in oxidative stress and a decrease in protein content, regardless of the increase in the activity of antioxidant enzymes. These results suggest the potentially dangerous effects of the chemicals' mixture combined with the temperature, on this species and its consumers, impacting the trophic chain, and consequently, the community structure and function.

Marine Macroalgae Polyphenols as Potential Neuroprotective Antioxidants in Neurodegenerative Diseases.

Polyphenols are beneficial natural compounds with antioxidant properties that have recently gain a lot of interest for their potential therapeutic applications. Marine polyphenols derived from marine macroalgae have been discovered to possess interesting antioxidant properties; therefore, these compounds can be included in several areas of drug development. Authors have considered the use of polyphenol extracts from seaweeds as neuroprotective antioxidants in neurodegenerative diseases. Marine polyphenols may slow the progression and limit neuronal cell loss due to their antioxidant activity; therefore, the use of these natural compounds would improve the quality of life for patients affected with neurodegenerative diseases. Marine polyphenols have distinct characteristics and potential. Among seaweeds, brown algae are the main sources of polyphenols, and present the highest antioxidant activity in comparison to red algae and green algae. The present paper collects the most recent in vitro and in vivo evidence from investigations regarding polyphenols extracted from seaweeds that exhibit neuroprotective antioxidant activity. Throughout the review, oxidative stress in neurodegeneration and the mechanism of action of marine polyphenol antioxidant activity are discussed to evidence the potential of algal polyphenols for future use in drug development to delay cell loss in patients with neurodegenerative disorders.

Ecotoxicological and biochemical effects of a binary mixture of pesticides on the marine diatom Thalassiosira weissflogii in a scenario of global warming.

Under the current scenario of global warming, it is ecologically relevant to understand how increased temperature influences the combined toxicity of pesticides to aquatic species. Hence, this work aims to: a) determine the temperature effect (15 °C, 20 °C and 25 °C) on the toxicity of two pesticides (oxyfluorfen and Copper (Cu)), on the growth of Thalassiosira weissflogii; b) assess whether temperature affects the type of toxicity interaction between these chemicals; and c) assess the temperature effect on biochemical responses (fatty acids (FA) and sugar profiles) of the pesticides on T. weissflogii. Temperature increased the tolerance of the diatoms to the pesticides with EC50 values between 3.176 and 9.929 µg L-1 for oxyfluorfen and 42.50-230.75 µg L-1 for Cu, respectively, at 15 °C and 25 °C. The mixtures toxicity was better described by the IA model, but temperature altered the type of deviation from dose ratio (15 °C and 20 °C) to antagonism (25 °C). Temperature, as well as the pesticide concentrations, affected the FA and sugar profiles. Increased temperature increased saturated FA and decreased unsaturated FA; it also affected the sugar profiles with a pronounced minimum at 20 °C. Results highlight effects on the nutritional value of these diatoms, with potential repercussion on food webs.

The Lethal and Sub-Lethal Effects of Fluorinated and Copper-Based Pesticides-A Review.

In recent decades, pollution levels have increased, mainly as a result of the intensive anthropogenic activities such industrial development, intensive agricultural practices, among others. The impact of metals and organic contaminants is, nowadays, a great concern to the scientific and political communities. Copper compounds are the main sold pesticides in Europe, as well as herbicides, including glyphosate. Diphenyl ethers are the second ones most sold. Glyphosate and copper compounds are intensively studied, but the opposite is seen in the case of diphenyl ethers, including fluorinated pesticides (e.g., oxyfluorfen). Some research has been performed to increase the knowledge about these contaminants, daily inputted on the aquatic systems and with dangerous effects at physical and biochemical levels on the organisms. A wide range of biomarkers (e.g., growth, survival, reproductive success, enzymatic activity, lipid metabolism) has been applied to determine the potential effects in many species. This review intends to: (a) perform a compilation of the knowledge in previous research about the action mode of organic (fluorinated-based herbicide) and inorganic (copper-based pesticides) contaminants; (b) carry out an information survey about the lethal and sub-lethal effects of the fluorinated-based pesticides, namely the oxyfluorfen and the copper-based pesticides, on aquatic species from different trophic levels, according to in vitro and in vivo studies; (c) understand the impact of oxyfluorfen and copper-based pesticides, considering their effects reported in in vitro studies and, simultaneously, the authorized concentrations by legal organizations and the effective concentrations of each pollutant found in the environment. The literature analyzed revealed noxious effects of Cu and oxyfluorfen to aquatic organisms, including freshwater and marine species, even when exposed to the reference as well as to environmental concentrations, thus highlighting the importance of more monitoring and ecotoxicological studies, to chemical pollutants and different species from different ecological niches, to sustain and improve the legislation.

Antiviral Activity and Mechanisms of Seaweeds Bioactive Compounds on Enveloped Viruses-A Review.

In the last decades, the interest in seaweed has significantly increased. Bioactive compounds from seaweed's currently receive major attention from pharmaceutical companies as they express several interesting biological activities which are beneficial for humans. The structural diversity of seaweed metabolites provides diverse biological activities which are expressed through diverse mechanisms of actions. This review mainly focuses on the antiviral activity of seaweed's extracts, highlighting the mechanisms of actions of some seaweed molecules against infection caused by different types of enveloped viruses: influenza, Lentivirus (HIV-1), Herpes viruses, and coronaviruses. Seaweed metabolites with antiviral properties can act trough different pathways by increasing the host's defense system or through targeting and blocking virus replication before it enters host cells. Several studies have already established the large antiviral spectrum of seaweed's bioactive compounds. Throughout this review, antiviral mechanisms and medical applications of seaweed's bioactive compounds are analyzed, suggesting seaweed's potential source of antiviral compounds for the formulation of novel and natural antiviral drugs.

Call the Eckols: Present and Future Potential Cancer Therapies.

In recent years, an increased interest in marine macroalgae bioactive compounds has been recorded due to their benefits to human health and welfare. Several of their bioactivities have been demonstrated, such as anti-inflammatory, antioxidant, anticarcinogenic, antibacterial and antiviral behavior. However, there still lacks a clear definition regarding how these compounds exert their bioactive properties. Of all the bioactive compounds derived from marine macroalgae, attention has been focused on phenolic compounds, specifically in phlorotannins, due to their potential for biomedical applications. Phlorotannins are a diverse and wide group of phenolic compounds, with several structural variations based on the monomer phloroglucinol. Among the diverse phlorotannin structures, the eckol-family of phlorotannins demonstrates remarkable bioactivity, notably their anti-tumoral properties. However, the molecular mechanisms by which this activity is achieved remain elusive and sparse. This review focuses on the described molecular mechanisms of anti-tumoral effects by the eckol family of compounds and the future prospects of these molecules for potential application in oncology therapies.

Seaweed Phenolics: From Extraction to Applications.

Seaweeds have attracted high interest in recent years due to their chemical and bioactive properties to find new molecules with valuable applications for humankind. Phenolic compounds are the group of metabolites with the most structural variation and the highest content in seaweeds. The most researched seaweed polyphenol class is the phlorotannins, which are specifically synthesized by brown seaweeds, but there are other polyphenolic compounds, such as bromophenols, flavonoids, phenolic terpenoids, and mycosporine-like amino acids. The compounds already discovered and characterized demonstrate a full range of bioactivities and potential future applications in various industrial sectors. This review focuses on the extraction, purification, and future applications of seaweed phenolic compounds based on the bioactive properties described in the literature. It also intends to provide a comprehensive insight into the phenolic compounds in seaweed.

Copper sulphate impact on the antioxidant defence system of the marine bivalves Cerastoderma edule and Scrobicularia plana.

Anthropogenic activities, such as agriculture and industrial activities, are a main source of pollution contributing for the degradation of water quality and thus affecting the living organisms of aquatic systems. Copper is widely used at these practices being often released into the aquatic systems and may cause negative effects in its communities. This study proposes to determine the effects of copper in the antioxidant defence system of two size classes (big and small sizes) of Scrobicularia plana and Cerastoderma edule, two marine bivalve species with commercial interest. It was observed the behaviour activity of the organisms during the exposure to copper sulphate (CS) and was determined the enzymatic activities of glutathione-S-transferases (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) (both selenium-dependent (SeGPx) and total (tGPx)) in the muscle tissue (foot). Lipid peroxidation (LPO) was evaluated through thiobarbituric acid reactive substances (TBARS) measurement in the foot. Changes in the behaviour and enzymatic activity were observed. Lipid peroxidation was observed at C. edule and S. plana big and small size classes, respectively, according to TBARS levels. The foot showed to be a good tissue to be used in biochemical analysis to detect the presence of toxicants.

Toxicity evaluation of three pesticides on non-target aquatic and soil organisms: commercial formulation versus active ingredient.

The Ecological Risk Assessment of pesticides requires data regarding their toxicity to aquatic and terrestrial non-target species. Such requirements concern active ingredient(s), generally not considering the noxious potential of commercial formulations. This work intends to contribute with novel information on the effects of short-term exposures to two herbicides, with different modes of action (Spasor, Stam Novel Flo 480), and an insecticide (Lannate), as well as to corresponding active ingredients (Glyphosate, Propanil and Methomyl, respectively). The microalga Pseudokirchneriella subcapitata (growth inhibition), the cladoceran Daphnia magna (immobilisation), and the earthworm Eisenia andrei (avoidance behaviour) were used as test species. Both herbicides were innocuous to all test organisms at environmentally realistic concentrations, except for Stam and Propanil (highly toxic for Pseudokirchneriella; moderately toxic to Daphnia). Lannate and Methomyl were highly toxic to Daphnia and caused Eisenia to significantly avoid the spiked soil at realistic application rates. The toxicity of formulations either overestimated (e.g. Stam/Propanil for P. subcapitata) or underestimated (e.g. Stam/Propanil for D. magna) that of the active ingredient.