Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus.

BACKGROUND: Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. METHODS AND FINDINGS: Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. CONCLUSIONS AND SIGNIFICANCE: Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

Effects of Temephos, Permethrin, and Eucalyptus nitens Essential Oil on Survival and Swimming Behavior of Aedes aegypti and Anopheles pseudopunctipennis (Diptera: Culicidae) Larvae.

An essential strategy to deal with mosquito-borne diseases is the control of larvae in their development sites. The mosquitoes Anopheles pseudopunctipennis (Theobald) (Diptera: Culicidae), a malaria vector, and Aedes aegypti (L.) (Diptera: Culicidae), vector of dengue, Zika, yellow fever, and chikungunya viruses, breed in very different habitats. Insecticide treatments of mosquito larvae focus mainly on their lethal effects. However, insecticide degradation or the poor dosage of larvicides will invariably lead to the sublethal exposure of a target (and nontarget) species, the nonlethal effects of these compounds may have important effects on vital insect activities, and therefore their evaluation is necessary. In this study, we assessed the survival and swimming behavior of larvae of Ae. aegypti and An. pseudopunctipennis exposed to increasing concentrations of three larvicides. We found that Ae. aegypti, was more sensitive to the larvicides than An. pseudopunctipennis, we also observed an overall decrease in the movement of those larvae of both species, which survive the treatments. This decrease might have ecological relevance in their natural habitats, increasing the chance to be predated and decreasing their ability to obtain food. Finally, this information will be valuable to assist authorities to make decisions in the implementation of further control programs.

Estudo do efeito agudo da fadiga na força de membros inferiores durante saltos com contramovimento / Estudio del efecto de la fatiga aguda en la fuerza del miembros inferiores durante saltos con contramovimiento / Acute fatigue effects on ground reaction force of lower limbs during countermovement jumps

Parameters associated with the performance of countermovement jumps were identified from vertical ground reaction force recordings during fatigue and resting conditions. Fourteen variables were defined, dividing the vertical ground reaction force into negative and positive external working times and times in which the vertical ground reaction force values were lower and higher than the participant's body weight. We attempted to explain parameter variations by considering the relationship between the set of contractile and elastic components of the lower limbs. We determined that jumping performance is based on impulsion optimization and not on instantaneous ground reaction force value: the time in which the ground reaction force was lower than the body weight, and negative external work time was lower under fatigue. The results suggest that, during fatigue, there is less contribution from elastic energy and from overall active state. However, the participation of contractile elements could partially compensate for the worsening of jumping performance.

Parâmetros associados com o desempenho do salto contramovimento foram identificados a partir de registros da força vertical em condições de fadiga e não fadiga. Quatorze variáveis foram definidas dividindo a força de reação vertical do solo em valores negativos e positivos de trabalho externo e o tempo em que os valores de força vertical foi menor e maior do que o peso corporal do indivíduo. As variações dos parâmetros foram avaliadas considerando a relação entre conjunto de elementos contráteis e elásticos nos membros inferiores. Determinou-se que o desempenho é baseado na otimização do impulso e não em um valor instantâneo da força; o tempo em que a força de reação do solo foi menor do que o peso corporal e o tempo de trabalho externo negativo são mais baixos sob fadiga. Os resultados sugerem que durante a fadiga há uma menor contribuição da energia elástica e do estado ativo de forma global, mas a participação de elementos contráteis poderia compensar parcialmente a queda de desempenho.

Se identificaron parámetros asociados con el desempeño de saltos con contramovimiento a partir de registros de fuerza vertical en condiciones de fatiga y no fatiga. Catorce variables fueron definidas dividiendo la componente vertical de la curva de fuerza de reacción del suelo en valores negativos y positivos de trabajo externo y tiempo en que los valores de fuerza resultaron menores o mayores que el valor del peso del individuo. Las variaciones de los parámetros fueron evaluadas considerando la relación entre el conjunto de elementos contráctiles y elásticos en los miembros inferiores. Se determinó que el desempeño está basado en la optimización del impulso y no en un valor instantáneo de fuerza; el tiempo en que la fuerza de reacción del suelo fue menor que el peso corporal y el tiempo de trabajo externo negativo resultaron más bajos en fatiga. Los resultados sugieren que durante la fatiga hay una menor contribución de la energía elástica y del estado activo de forma global, pero la participación de elementos contráctiles podría compensar parcialmente la caída en el desempeño.