Paclobutrazol reduces growth and increases chlorophyll indices and gas exchanges of basil (Ocimum basilicum).

Basil (Ocimum basilicum) is a medicinal, ornamental and aromatic plant, however, its size can be an obstacle to its commercialization as a potted ornamental plant. Paclobutrazol (PBZ) is a substance that can retard plant growth by inhibiting the synthesis of gibberellins. The objective of this work was to evaluate the effect of paclobutrazol on growth regulation and gas exchange of basil (var. Cinnamon). The experiment was carried out in a completely randomized design with five treatments (PBZ doses: 0, 2.5, 5, 7.5 and 10 mg L-1), with eight replicates. Growth (plant height, number of leaves, stem diameter, leaf dry mass, stem dry mass, inflorescence dry mass, and total), growth rates (leaf mass ratio, stem mass ratio, inflorescence mass ratio, and robustness quotient), chlorophyll indices, gas exchange (gs, A, E, Ci, WUE, iWUE and iCE) were evaluated. Paclobutrazol reduced the growth of basil plants and increased the chlorophyll indices, A, gs, and WUE. Paclobutrazol can be used to regulate plant growth of basil plants var. Cinnamon, without altering its physiological and ornamental characteristics.

OPEN RYGB LONG-TERM COMPLICATIONS: VENTRAL HERNIA - REPORT ON A 10-YEAR SINGLE-CENTER EXPERIENCE.

Aim of Study - we hypothesize if there are any apparent risk stratifications to the abdominal wall compromise after open Roux-en-Y gastric bypass (RYGB) surgery and if the outcomes of retromuscular sublay mesh repair using Rives/Stoppa's technique were different from the literature. Description of a 10-year experience of a single center using sublay retromuscular mesh hernia repair in patients with ventral hernia after RYGB, including a total of 189 patients. 149 (78.8%) patients were women. Mean age of 47.3±10.9 years. 171 (90.48%) patients had at least one comorbidity, being systemic arterial hypertension the most prevalent (74.26%). The most frequent location of hernias was in the epigastric region with 125 cases (66.1%), followed by umbilical, inferior and subcostal (28.4%; 3.6%; 1.8%). The mean BMI of patients undergoing bariatric surgery was 50.2±6.6 kg/m². 112 (59.26%) patients within the ventral hernia sample had a BMI higher than 50 kg/m² before the bariatric surgery. The average length-of-stay at the hospital was 2.6 days. There were 17 (9%) cases of hernia recurrence. BMI>50 kg/m² and female sex may be risk factor to incisional ventral hernia in patients after open Roux-en-Y gastric bypass. Retromuscular sublay mesh appliance using Rives/Stoppa's technique had low length-of-stay of hospitalization and hernia recurrence compared to the literature.

Critical view of robotic-assisted transverse abdominal release (r-TAR).

INTRODUCTION: Establishing straightforward and reproducible steps to describe the technique performed with the aid of the robotic system for complex hernia surgery is key for good outcomes. Even using the description of open surgery as a parameter for performing the robotic technique, it is important to stress the particularities of this access. To describe the steps to perform robotic-assisted TAR (r-TAR) in a standardized technique, with a critical and safe view of all the anatomical structures. DESCRIPTION OF THE TECHNIQUE: We defined 8 landmarks for the critical view of safety in r-TAR which include: (1) patient position, trocar and docking; (2) posterior rectus sheath mobilization; (3) transversus abdominis release (TAR)-Top-down technique; (4) transversus abdominis release (TAR)-bottom-up technique and mesh insertion; (5) contralateral trocar insertion and redocking, 6) posterior sheath closure; (7) final mesh positioning; and (8) anterior defect closure and drains. DISCUSSION: Complex hernia surgery using a robotic-assisted posterior component separation requires well-established steps so the procedure can be reproducible and achieve better results.