RESUMO
In this study, we surveyed six species of cockroaches, two synanthropic (i.e. ecologically associated with humans) and four wild, for intestinal trypanosomatid infections. Only the wild cockroach species were found to be infected, with flagellates of the genus Herpetomonas. Two distinct genotypes were documented, one of which was described as a new species, Herpetomonas tarakana sp. n. We also propose a revision of the genus Herpetomonas and creation of a new subfamily, Phytomonadinae, to include Herpetomonas, Phytomonas, and a newly described genus Lafontella n. gen. (type species Lafontella mariadeanei comb. n.), which can be distinguished from others by morphological and molecular traits.
Assuntos
Baratas/parasitologia , Trypanosomatina/classificação , Animais , Biodiversidade , República Tcheca , DNA de Protozoário/genética , Genótipo , Microscopia Eletrônica de Transmissão , Filogenia , Reação em Cadeia da Polimerase/métodos , RNA Ribossômico 18S/genética , Análise de Sequência de DNA , Eslováquia , Trypanosomatina/genética , Trypanosomatina/isolamento & purificação , Trypanosomatina/ultraestruturaRESUMO
The class Kinetoplastea encompasses both free-living and parasitic species from a wide range of hosts. Several representatives of this group are responsible for severe human diseases and for economic losses in agriculture and livestock. While this group encompasses over 30 genera, most of the available information has been derived from the vertebrate pathogenic genera Leishmaniaand Trypanosoma. Recent studies of the previously neglected groups of Kinetoplastea indicated that the actual diversity is much higher than previously thought. This article discusses the known segment of kinetoplastid diversity and how gene-directed Sanger sequencing and next-generation sequencing methods can help to deepen our knowledge of these interesting protists.