Emilia sonchifolia leaf extract-mediated green synthesis, characterization, in vitro biological activities, photocatalytic degradation and in vivo Danio rerio embryo toxicity of copper nanoparticles.

The green-mediated synthesis of copper nanoparticles is of great interest in nanotechnology and is regarded as a low-cost and environmentally beneficial method. Herein, Emilia sonchifolia leaf extract was employed as a reducing and capping agent for the green production of copper nanoparticles. In this work, we focused on the in vivo and in vitro biological studies of copper nanoparticles, which were evaluated in zebrafish (Danio rerio) embryos. The biological effects from the in vitro studies of the copper nanoparticles included cytotoxicity (in human cells) and anti-diabetic, anti-inflammatory, and antibacterial activity. Furthermore, the effectiveness of the greenly produced copper nanoparticles for photocatalysis was also evaluated, and then SEM-EDX, FTIR, XRD, TGA and UV-vis spectroscopy were used to characterise the copper nanoparticles. The results of the toxicity test on zebrafish embryos demonstrated that the green-produced copper nanoparticles had a significantly low harmful effect. According to the results, the copper nanoparticles showed dose-dependent cytotoxicity against human keratinocytes (HaCaT) and human breast cancer cells (MCF-7), which was higher than that of the Emilia sonchifolia leaf extract. The green copper nanoparticles also demonstrated more potent anti-inflammatory, antibacterial and anti-diabetic properties. In the photocatalytic experiment, the produced copper nanoparticles successfully degraded the organic methylene blue dye. Thus, it can be concluded that copper nanoparticles can be employed for drug administration in both in vitro and in vivo settings in biomedical applications. Additionally, as catalysts, these copper nanoparticles can be employed for the removal of organic dyes.

Curcumin-Chitosan Nanocomposite Formulation Containing Pongamia pinnata-Mediated Silver Nanoparticles, Wound Pathogen Control, and Anti-Inflammatory Potential.

BACKGROUND: Because of its diverse range of use in several ethics of diagnosis and care of multiple diseases, nanotechnology has seen remarkable growth and has become a key component of medical sciences. In recent years, there has been rapid advancement in medicine and biomaterials. Nanomedicine aids in illness prevention, diagnosis, monitoring, and treatment. AIM: The purpose of this work is to evaluate the antibacterial, anti-inflammatory, and cytotoxic capabilities of green produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) against wound pathogenic as reducing agents. MATERIALS AND METHODS: The plant extract of Pongamia pinnata, silver nanoparticles, and its based curcumin nanoformulations was studied in this study utilizing UV visible spectrophotometer, selected area electron diffraction (SAED), and TEM. Anti-inflammatory, antimicrobial, and cytotoxic tests were performed on silver nanoparticles with the addition of curcumin-assisted chitosan nanocomposite (SCCN). Furthermore, these produced nanocomposites were coated on clinical silk and tested for antibacterial activity. RESULTS: The produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) has significant antibacterial activities against Pseudomonas aeruginosa and staphylococcus aureus. They are as well as possess anti-inflammatory activity and furthermore prove to be biocompatible. CONCLUSION: This advancement in the field of biomaterials, which means nanocomposite, not only helps to reduce the harmful effects of pathogenic organisms while representing an environmentally benign material but it also shows to be a material with zero danger to humans and the environment.