RESUMO
BACKGROUND AND AIM: Inflammatory cells involved in the pathophysiology of asthma express nicotinic receptor. Therefore 1,1 dimethyl(-4-)phenylpiperazinium (DMPP) in two doses were compared to dexamethasone in asthmatic guinea pigs. MATERIALS AND METHODS: Six groups were included; Normal control and five asthmatic (OVA-sensitized and challenged) groups; which were treated for 10 days as follows: two vehicles, dexamethasone (DEXA, 1 mg/kg) and DMPP (0.4 and 0.8 mg/kg) groups. Pulmonary functions and airway hyper-responsiveness were assessed. Leukocytic count, tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6) and immunoglobulin E (IgE) were measured in both blood and bronchoalveolar lavage fluid (BALF). Histopathological examination of the lung tissues was conducted. RESULTS: Asthmatic untreated animals exhibited significant increase in early and late airway resistance (RxV) and airway hyper-responsiveness, with reduction in tidal volume. Both blood and BALF showed significant increase in total leukocytic count (TLC), eosinophils, lymphocytes, monocytes, TNF-α, IL-6 and IgE with significant decrease in neutrophils. Airway inflammatory cell infiltration and smooth muscle thickness significantly increased. DMPP 0.4 mg/kg significantly decreased late phase RXV, TLC, BALF lymphocytes, TNF-α, smooth muscle thickness and increased neutrophils in BALF over both DEXA and DMPP 0.8 mg/kg. Moreover, DMPP 0.4 mg/kg significantly decreased IL-6 and BALF eosinophils than DMPP 0.8 mg/kg and decreased serum IgE and parenchymal inflammatory infiltration than DEXA. CONCLUSIONS: Low dose DMPP has more anti-inflammatory effect than a high dose in most parameters and sometimes than dexamethasone. Cholinergic anti-inflammatory pathway may therefore represent a potential drug target for allergic asthma. The dose related effect of DMPP and the mechanism underlying this effect require further evaluation.
Assuntos
Anti-Inflamatórios/farmacologia , Asma/induzido quimicamente , Asma/tratamento farmacológico , Dexametasona/farmacologia , Iodeto de Dimetilfenilpiperazina/farmacologia , Ovalbumina/efeitos adversos , Animais , Antiasmáticos/farmacologia , Asma/metabolismo , Líquido da Lavagem Broncoalveolar/química , Modelos Animais de Doenças , Eosinófilos/efeitos dos fármacos , Eosinófilos/metabolismo , Cobaias , Imunoglobulina E/metabolismo , Interleucina-6/metabolismo , Contagem de Leucócitos/métodos , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismo , Masculino , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Músculo Liso/efeitos dos fármacos , Músculo Liso/metabolismo , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
BACKGROUND: Several epidemiologic studies have suggested the association between therapy with proton pump inhibitors (PPIs) and bone fractures. AIM: This study aimed at evaluating the effect of omeprazole on bone in normal and ovariectomized Wistar rats and the possible mechanisms involved. MATERIALS AND METHODS: 56 rats were divided into 3 main groups. Normal group; further subdivided into normal control group and two groups which were treated with omeprazole in two doses (20, 40 mg/kg/day i.p). Sham operated group. Ovariectomized group; further subdivided into ovariectomized control group, and two groups which were treated with omeprazole in two doses (20, 40 mg/kg/day i.p). Rats were treated for the last 4 weeks of the total 8 weeks of the experiment. Urine hydroxyproline, serum osteocalcin, TNF-α and IL-6 and bone mineral content were assessed. Omeprazole effects on the endothelial dependent and independent relaxation were determined. RESULTS: Omeprazole in normal and ovariectomized rats produced significant reduction in bone formation, tibia calcium content and serum TNF-α and IL-6. Omeprazole in ovariectomized rats produced a dose dependent decrease in bone resorption. Isolated aortic rings from ovariectomized/omeprazole treated rats exhibited reversal of the endothelial dysfunction that observed with ovariectomized rats. CONCLUSIONS: PPIs might induce both positive and negative effects on bone remodeling. Although these drugs might have the potential to inhibit bone resorption, through suppression of pro-inflammatory cytokines and improvement of endothelial function, yet these effects are counteracted by their inhibitory effects on the gastric proton pump with reduction in calcium absorption and bone formation.