Differential Effect of 4H-Benzo[d] [1, 3]oxazines on the Proliferation of Breast Cancer Cell Lines.

BACKGROUND: A family of 4H-benzo[d][1,3]oxazines were obtained from a group of N-(2-alkynyl)aryl benzamides precursors via gold(I) catalysed chemoselective 6-exo-dig C-O cyclization. METHOD: The precursors and oxazines obtained were studied in breast cancer cell lines MCF-7, CAMA-1, HCC1954 and SKBR-3 with differential biological activity showing various degrees of inhibition with a notable effect for those that had an aryl substituted at C-2 of the molecules. 4H-benzo[d][1,3]oxazines showed an IC50 rating from 0.30 to 157.4 µM in MCF-7, 0.16 to 139 in CAMA-1, 0.09 to 93.08 in SKBR-3, and 0.51 to 157.2 in HCC1954 cells. RESULTS: We observed that etoposide is similar to benzoxazines while taxol effect is more potent. Four cell lines responded to benzoxazines while SKBR-3 cell line responded to precursors and benzoxazines. Compounds 16, 24, 25 and 26 have the potent effect in cell proliferation inhibition in the 4 cell lines tested and correlated with oxidant activity suggesting a possible mechanism by ROS generation. CONCLUSION: These compounds represent possible drug candidates for the treatment of breast cancer. However, further trials are needed to elucidate its full effect on cellular and molecular features of cancer.

Common bean seeds from plants subjected to severe drought, restricted- and full-irrigation regimes show differential phytochemical fingerprint.

Common bean is rich in phytochemicals like polyphenols, saponins, and steroidal glycosides, but its crop is greatly affected by drought. Thus, it is necessary to identify the irrigation regimes that improves its phytochemical profile without affecting seed yield. The aim of this study was to evaluate the differential effect of severe drought (SD), restricted irrigation (RI), and full irrigation (FI) on the phytochemical fingerprint of bean seeds through liquid chromatography-mass spectrometry and chemometric approach. Partial least square-discriminant analysis (PLS-DA) indicated that seeds from plants grown under RI where differentiated from those grown under FI and SD due to their high content of quercetin 3-O-rhamnoside and luteolin 7-O-glucoside. Regarding seed yield, no significant (p < 0.05) differences were observed between RI and FI, whereas SD decreased (p < 0.05) seed yield as compared to FI (47%). These results suggest that mild hydric stress (RI) can be used to induce phytochemicals without affecting seed yield.