RESUMO
Crumb rubber derived from end-of-life tires (ELTs) is frequently used as infill of synthetic turf pitches, promoting circular economy. Although important to reduce the accumulation of waste, the use of recycled ELTs can be a problem to human health and the environment, both by direct contact during pitch use and by the release of these elements to the surroundings, mostly via volatilization and leaching. The present study aimed to evaluate the distribution of metals in ELT-derived crumb rubber collected in artificial turf worldwide and assess possible trends by country, pitch age and type (indoor vs. outdoor). The concentration ranges observed are very wide, especially in outdoor fields and for the most abundant metals, namely Zn (2989-5246 mg/kg), Fe (196-5194 mg/kg), Mg (188-1795 mg/kg) and Al (159-1882 mg/kg). For Zn, the levels were mostly above the safe limits set in European directives for relatable matrices (soils and toy materials), and the same happened for Pb, a much more toxic metal at lower concentrations. A multi-pathway human exposure study was also performed, and the risk assessment shows non-carcinogenic and carcinogenic risks from accidental crumb rubber ingestion (with Cr and Pb as major contributors) above the acceptable values for all the receptors except adult bystanders, with a higher significance to younger individuals. These results bring a different perspective regarding most of the studies reporting low risks related with exposure to metals in crumb rubber.
Assuntos
Metaloides , Metais Pesados , Adulto , Humanos , Chumbo , Reciclagem , Medição de Risco , Borracha/toxicidadeRESUMO
Volatile methylsiloxanes (VMSs) constitute a group of compounds used in a great variety of products, particularly personal care products. Due to their massive use, they are continually discharged into wastewater treatment plants and are increasingly being detected in wastewater and in the environment at low concentrations. The aim of this work was to develop and validate a fast and reliable methodology to screen seven VMSs in water samples, by headspace solid-phase microextraction (HS-SPME) followed by gas chromatography with flame ionization detection (GC-FID). The influence of several factors affecting the extraction efficiency was investigated using a design of experiments approach. The main factors were selected (fiber type, sample volume, ionic strength, extraction and desorption time, extraction and desorption temperature) and optimized, employing a central composite design. The optimal conditions were: 65 µm PDMS/Divinylbenzene fiber, 10 mL sample, 19.5% NaCl, 39 min extraction time, 10 min desorption time, and 33 °C and 240 °C as extraction and desorption temperature, respectively. The methodology was successfully validated, showing low detection limits (up to 24 ng/L), good precision (relative standard deviations below 15%), and accuracy ranging from 62% to 104% in wastewater, tap, and river water samples.
Assuntos
Siloxanas/análise , Microextração em Fase Sólida/métodos , Poluentes Químicos da Água/análise , Ionização de Chama , Água Doce/química , Cromatografia Gasosa-Espectrometria de Massas , Limite de Detecção , Rios/química , Águas Residuárias/químicaRESUMO
A headspace solid-phase microextraction (HS-SPME) method was developed using the metal-organic framework (MOF) CIM-80(Al) as extraction phase and in combination with gas chromatography-mass spectrometry (GC-MS) for the simultaneous determination of 6 methylsiloxanes and 7 musk fragrances in different environmental waters. The chromatographic separation was optimized in different GC instruments equipped with different detectors, allowing the correct separation and identification of the compounds. The HS-SPME method was optimized using a Box-Behnken experimental design, while the validation was carried out together with the most suitable commercial fiber (divinylbenzene/polydimethylsiloxane) for comparison purposes. The MOF-based coating was particularly efficient for the determination of volatile methylsiloxanes, showing moderately lower limits of detection (of 0.2 and 0.5 µg L-1versus 0.6 µg L-1 for cyclic methylsiloxanes) and slightly better precision (relative standard deviation values lower than 17% versus 22%) than the commercial coating, while avoiding the cross-contamination issues associated to the polymeric composition of commercial fibers. The method was applied for the analysis of seawater and wastewater samples, allowing the quantification of several analytes and the assessment of matrix effects. The proposed HS-SPME method using the CIM-80(Al) fiber constitutes a more environmentally friendly, simpler, and efficient strategy in comparison with other sample preparation methods using different extraction techniques, while the use of a MOF as fiber sorbent constitutes a potential alternative to exploit the features of SPME for the challenging environmental monitoring of these compounds.
RESUMO
A Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) methodology followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis was developed to extract thirteen synthetic musk compounds (SMCs: cashmeran, celestolide, phantolide, traseolide, galaxolide, tonalide, musk ambrette, musk xylene, musk ketone, musk tibetene, musk moskene, ethylene brassylate and exaltolide) and six ultraviolet-filters (UVFs: 2-ethylhexyl 4-dimethylaminobenzoate, 3-(4'-methylbenzylidene) camphor, 2-ethylhexyl 4-methoxycinnamate, 2-ethylhexyl 2-cyano-3,3-diphenylacrylate, benzophenone and drometrizole trisiloxane) from tomatoes. The proposed methodology was optimized: 2â¯g of freeze-dried tomato was extracted with 4â¯mL of water and 10â¯mL of ethyl acetate, adding 6â¯g of MgSO4 and 1.5â¯g of NaCl, then a dispersive solid-phase extraction was performed using 3â¯g of MgSO4, 300â¯mg of primary-secondary amino adsorbent (PSA) and 300â¯mg of octadecyl-silica (C18). Validation delivered recoveries between 81 (celestolide) and 119% (musk tibetene), with relative standard deviations <10%. The instrumental limit of detection varied from 0.02 (2-ethylhexyl 4-methoxycinnamate) to 3.00â¯pg (exaltolide and musk xylene). Regarding the method quantification limits, it ranged between 0.4 (celestolide) and 47.9â¯ngâ¯g-1 dw (exaltolide). The method was applied to different varieties of tomatoes (Solanum lycopersicum), revealing UVFs and SMCs between 1 and 210â¯ngâ¯g-1 dw. Higher concentrations were found for benzophenone (29-210â¯ngâ¯g-1 dw) and galaxolide (9-53â¯ngâ¯g-1 dw). The risk associated to the ingestion of contaminated tomatoes has also been estimated, showing that a potential health risk is unlikely.
Assuntos
Ácidos Graxos Monoinsaturados/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Solanum lycopersicum/química , Poluentes Químicos da Água/análise , Benzofenonas/análise , Benzopiranos/análise , Dinitrobenzenos/análise , Indanos/análise , Extração em Fase Sólida , Espectrometria de Massas em Tandem , Tetra-Hidronaftalenos/análise , Xilenos/análiseRESUMO
A methodology based on solid-phase microextraction (SPME) followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the simultaneous analysis of different families of personal care products (PCPs) including fragrance allergens, synthetic musks, phthalates, antioxidants and UV filters in continental waters. The main parameters affecting SPME procedure were optimized by an ANOVA study. The final selected conditions comprised the use of 10â¯mL of sample with 20% (w/v) of sodium chloride (NaCl), polydimtehylsiloxane/divinylbenzene (PDMS/DVB) fiber and 20â¯min of extraction time at 100⯰C in the head-space mode. Good linearity (R2>0.9925), quantitative recoveries (>79%), and precision (RSDâ¯<â¯15%) were achieved for all compounds under the optimal conditions. Limits of quantification (LOQs) at the sub and low ng L-1 were obtained. The validated methodology was successfully applied to the analysis of river water samples from the North Portuguese coast allowing the determination of five different families of PCPs, including a total of 43 compounds in a single chromatographic run within 23â¯min.
Assuntos
Cosméticos/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Microextração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos , Poluentes Químicos da Água/análise , Limite de DetecçãoRESUMO
An analytical method was developed for the simultaneous analysis of 19 emergent compounds in water matrices, six UV-filters (UVFs), five nitro, six polycyclic and two macrocyclic musks. The target compounds were extracted by a dispersive liquid-liquid microextraction (DLLME) approach, using 2-propanol as the dispersive solvent and 1,1,2-trichloroethane as the extractant solvent. The extracts were then analysed by gas chromatography tandem mass-spectrometry (GC-MS/MS). This methodology was successfully validated for the analyses of the target compounds in five types of aqueous samples (tap, river and sea water and influent and effluent wastewater). Recoveries of the analytes based on the surrogate correction ranged from 80 to 120%, with a good repeatability (relative standard deviations less than 10%). The method limit of detection ranges from 0.1 ng L-1 (octocrylene (OC), celestolide (ADBI)) to 20.0 ng L-1 (benzophenone (BZ)). Both UVFs and synthetic musk compounds (SMCs) were detected in all matrices. Higher concentrations were found in wastewaters (total mean concentration in influents of 6248 ng L-1 and 3856 ng L-1 in effluents), followed by river water (159 ng L-1). Only BZ was detected in one of the analysed seawater samples and none of the compounds were detected in tap water. The most detected UVFs among all matrices were BZ and drometrizole (DTS) and tonalide (AHTN) and galaxolide (HHCB) within the SMCs class. Among all matrices, wastewater was the one with higher number of compounds found per sample (both UVFs and SMCs).
Assuntos
Ácidos Graxos Monoinsaturados/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Microextração em Fase Líquida/métodos , Protetores Solares/análise , Poluentes Químicos da Água/análise , Água Potável/química , Perfumes/análise , Água do Mar/química , Espectrometria de Massas em Tandem , Águas Residuárias/químicaRESUMO
A Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) methodology followed by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) analysis was developed to extract synthetic musk compounds (SMCs) (6 polycyclic, 2 macrocyclic and 5 nitro musks) and ultraviolet-filters (UVFs) (6 compounds) from sludge. This methodology fills a gap in the literature, since the proposed technique does not require specific equipment, nor large amounts of solvents, sorbents and time to extract SMCs and UVFs from sludge. To optimize this new methodology, a design of experiments (DoE) approach was used, applying first a screening design (SD) and then a central composite design (CCD). The best conditions achieved to extract these 19 compounds simultaneously were: 500â¯mg freeze dried sludge, 2.5â¯min of vortex and 15â¯min ultrasound and the use of a QuEChERS for the dispersive solid-phase extraction (d-SPE) containing 500â¯mg MgSO4, 410â¯mg C18 and 315â¯mg PSA. Then, this methodology was successfully validated. Recoveries of the target compounds ranged from 75% (cashmeran, DPMI) to 122% (2ethylhexyl 4methoxycinnamate, EHMC), with good repeatability (relative standard deviationâ¯<â¯10%). The instrumental detection limits (IDLs) and quantification (IQLs) varied from 0.001â¯pg (musk moskene, MM) to 7.5â¯pg (musk xylene, MX) and from 0.003 (MM) to 25â¯pg (MX), respectively. The method detection and quantification limits (MDLs and MQLs) ranged between 0.5 (DPMI) and 1394 (exaltolide, EXA) ng/g dw and 2 and 4648â¯ng/g-dw, respectively. Both SMCs and UVFs were detected in all sludge samples analysed. Higher concentrations were found for octocrylene (OC: maximum value of 115,486â¯ng/g-dw) followed by galaxolide (HHCB: 81,771â¯ng/g-dw). Only the nitro musks ambrette, xylene, moskene and tibetene and macrocyclic musk ethylene brassylate (EB) were not detected in any sample.
RESUMO
A methodology based on solid-phase microextraction (SPME) followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) has been developed for the simultaneous analysis of eleven multiclass ultraviolet (UV) filters in beach sand. To the best of our knowledge, this is the first time that this extraction technique is applied to the analysis of UV filters in sand samples, and in other kind of environmental solid samples. Main extraction parameters such as the fibre coating, the amount of sample, the addition of salt, the volume of water added to the sand, and the temperature were optimized. An experimental design approach was implemented in order to find out the most favourable conditions. The final conditions consisted of adding 1â¯mL of water to 1â¯g of sample followed by the headspace SPME for 20â¯min at 100⯰C, using PDMS/DVB as fibre coating. The SPME-GC-MS/MS method was validated in terms of linearity, accuracy, limits of detection and quantification, and precision. Recovery studies were also performed at three concentration levels in real Atlantic and Mediterranean sand samples. The recoveries were generally above 85% and relative standard deviations below 11%. The limits of detection were in the pg g-1 level. The validated methodology was successfully applied to the analysis of real sand samples collected from Atlantic Ocean beaches in the Northwest coast of Spain and Portugal, Canary Islands (Spain), and from Mediterranean Sea beaches in Mallorca Island (Spain). The most frequently found UV filters were ethylhexyl salicylate (EHS), homosalate (HMS), 4-methylbenzylidene camphor (4MBC), 2-ethylhexyl methoxycinnamate (2EHMC) and octocrylene (OCR), with concentrations up to 670â¯ng g-1.
Assuntos
Monitoramento Ambiental/métodos , Cromatografia Gasosa-Espectrometria de Massas , Sedimentos Geológicos/química , Microextração em Fase Sólida , Protetores Solares/análise , Oceano Atlântico , Monitoramento Ambiental/instrumentação , Mar Mediterrâneo , Portugal , Água do Mar/química , Espanha , Protetores Solares/isolamento & purificação , Poluentes Químicos da Água/análiseRESUMO
UV-filters are a group of compounds which have been massively used in the past years due to the recent concerns with sunburns, premature skin ageing and the risk of developing skin cancer, related to sun exposure. At the moment, these compounds have been identified by the scientific community as emerging pollutants, due to their persistence in the environment, potential to accumulate in biota and potential threat as endocrine disruptors. At some point, the majority of sunscreens will find their way into wastewater (due to bathing and washing activities) and because wastewater treatment plants (WWTPs) are not able to remove and/or degrade them, consequently they find their way into rivers, lakes and ocean, so it is not surprising that UV-filters are found in the environment. Therefore, wastewater treatment plants should be the focus of the scientific community aiming to better understand the fate of the UV-filters and develop new technologies to remove them from wastewater and sludge. This review, aims to provide the current state of the art in the occurrence and fate of UV-filters in wastewater treatment plants and how the technologies that are being used are successfully removing these compounds from both wastewater and sludge.
Assuntos
Disruptores Endócrinos/análise , Protetores Solares/análise , Águas Residuárias/química , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Biota/efeitos dos fármacos , Disruptores Endócrinos/toxicidade , Monitoramento Ambiental , Humanos , Esgotos/análise , Protetores Solares/toxicidade , Poluentes Químicos da Água/toxicidadeRESUMO
A rapid and simple method for the simultaneous determination of twelve synthetic musks in water samples, using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) coupled with gas chromatography-mass spectrometry (GC-MS) was successfully developed. The influence of seven factors (volume of the extraction solvent and disperser solvent, sample volume, extraction time, ionic strength, type of extraction and disperser solvent) affecting the UA-DLLME extraction efficiency was investigated using a screening design. The significant factors were selected and optimised employing a central composite design: 80 µL of chloroform, 880 µL of acetonitrile, 6 mL of sample volume, 3.5% (wt) of NaCl and 2 min of extraction time. Under the optimised conditions, this methodology was successfully validated for the analysis of 12 synthetic musk compounds in different aqueous samples (tap, sea and river water, effluent and influent wastewater). The proposed method showed enrichment factors between 101 and 115 depending on the analyte, limits of detection in the range of 0.004-54 ng L(-1) and good repeatability (most relative standard deviation values below 10%). No significant matrix effects were found, since recoveries ranged between 71% and 118%. Finally, the method was satisfactorily applied to the analysis of five different aqueous samples. Results demonstrated the existence of a larger amount of synthetic musks in wastewaters than in other water samples (average concentrations of 2800 ng L(-1) in influent and 850 ng L(-1) in effluent). Galaxolide, tonalide and exaltolide were the compounds most detected.
Assuntos
Ácidos Graxos Monoinsaturados/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Microextração em Fase Líquida/métodos , Perfumes/análise , Ondas Ultrassônicas , Água/análise , Ácidos Graxos Monoinsaturados/síntese química , Perfumes/síntese química , Poluentes Químicos da Água/análiseRESUMO
Vegetation is a useful matrix for the quantification of atmospheric pollutants such as semi-volatile organic compounds (SVOCs). In particular, pine needles stand out as effective biomonitors due to the excellent uptake properties of their waxy layer. Having previously validated an original and reliable method to analyse pesticides in pine needles, our work team set the objective of this study to determine the levels of 18 pesticides in Pinus pinea needles collected in 12 different sampling sites in Portugal. These compounds were selected among a total of 70 pesticides by previous chemical scoring, developed to assess their probability to occur in the atmosphere. The risk of exposure was evaluated by the binomial chemical score/frequency of occurrence in the analysed samples. Levels and trends of the chemical families and target of the pesticides were obtained regarding the type of land occupation of the selected sites, including the use of advanced statistics (principal component analysis, PCA). Finally, some correlations with several characteristics of the sampling sites (population, energy consumption, meteorology, etc.) were also investigated.
Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Praguicidas/análise , Pinus/química , Folhas de Planta/química , Poluição do Ar/estatística & dados numéricos , Portugal , Análise de Componente Principal , Medição de RiscoRESUMO
Polycyclic aromatic hydrocarbons (PAHs) are considered priority compounds due to their toxic and carcinogenic nature. The concern about water contamination and the consequent human exposure has encouraged the development of new methods for PAHs removal. The purpose of this work was to study the feasibility of a degradation process of benzo(a)pyrene (BaP) in aqueous matrices by oxidation with Fenton reagent. A laboratory unit was designed to optimize the factors which may influence the process: pH (3.5 to 6.0), temperature (30 to 70 degrees C), H(2)O(2) (20 to 150 mg L(-1)), Fe(2+) concentration (2.75 to 5.50 mg L(-1)), and the initial concentration of the pollutant (10 to 100 mug L(-1)). The pH did not influence significantly the results in the range studied. An increase in temperature from 30 to 70 degrees C improved the removal efficiency from 90% to 100%. The same effect was observed for ferrous ion concentrations from 2.75 to 5.50 mg L(-1) (increase from 78% to 100% removal). The H(2)O(2) concentration played a double role during the process: from 20 to 50 mg L(-1) an increase in the removal efficiency was achieved, but for higher concentrations (>50 mg L(-1)) the degradation is lower. This study proved that the degradation of benzo(a)pyrene by Fenton's reagent is a viable process.