Assuntos
Canais de Potássio Éter-A-Go-Go/genética , Esquizofrenia/genética , Animais , Encéfalo/fisiopatologia , Cognição , Canal de Potássio ERG1 , Emoções , Predisposição Genética para Doença , Hipocampo/fisiopatologia , Homeostase , Humanos , Transtornos Mentais/genética , Transtornos Mentais/psicologia , Polimorfismo de Nucleotídeo Único , Primatas , RNA Mensageiro/genética , Roedores , Psicologia do EsquizofrênicoRESUMO
A rare case of EBV encephalitis initially diagnosed as Herpes simplex infection is presented to highlight the importance of EBV specific intrathecal ELISA and liquor PCR based differential diagnosis when Herpes simplex encephalitis specific clinical symptoms, neuroimaging signs and electroencephalographic features are present. The case report also suggests that acyclovir treatment might be beneficial for the long term outcome in adult EBV encephalitis patients.
Assuntos
Encefalite por Herpes Simples/diagnóstico , Encefalite Viral/virologia , Infecções por Vírus Epstein-Barr/diagnóstico , Herpesvirus Humano 4/isolamento & purificação , Aciclovir/uso terapêutico , Adulto , Líquido Cefalorraquidiano/virologia , Diagnóstico Diferencial , Encefalite Viral/tratamento farmacológico , Ensaio de Imunoadsorção Enzimática/métodos , Infecções por Vírus Epstein-Barr/tratamento farmacológico , Feminino , Cabeça/diagnóstico por imagem , Humanos , Reação em Cadeia da Polimerase/métodos , Radiografia , Resultado do TratamentoRESUMO
This study examines the hypothesis that neuronal infectivity and the spreading of the pseudorabies virus (PRV) through the synapses in the central nervous system (CNS) are influenced by the oestrogen levels. The arcuate nucleus (ARC) and the subfornical organ (SFO) were chosen as models for analysis; the neurons in both structures possess oestrogen receptors and are mutually connected. A genetically engineered pseudorabies virus (Ba-DupLac) was used as a transneuronal tract tracer. This virus is taken up preferably by axon terminals, and transported very specifically through the synapses in a retrograde manner. Ba-DupLac was injected into the ARC of rats, followed by monitoring of the PRV-immunoreactivity (PRV-IR) in the SFO 72 h following inoculation. We found no PRV immunolabelling in the SFO of ovariectomized (OVX) rats, or in those OVX animals that received oestrogen shortly (4 h) before PRV infection (OVX + E 4 h). In contrast, in those OVX animals that received oestrogen 12 h before PRV infection (OVX + E 12 h), and also in intact control animals, PRV-IR was demonstrated in the SFO in all cases. Surprisingly, a reverse labelling was observed in the OVX rats; PRV-IR appeared in the pyriform cortex, whereas PRV-IR could not be detected in the control and OVX + E 12 h animals. As far as we are aware, this is the first study to demonstrate that transneuronal PRV labelling depends on the effects of oestrogen on certain CNS structures and connections.