RESUMO
A case study of the bioremediation of groundwater contaminated with trichloroethene (TCE) was conducted using the biostimulation agent, BD-1. TCE levels were monitored by gas chromatography-mass spectroscopy. Total organic carbon (TOC) and volatile fatty acids (VFAs) were analyzed to investigate the environmental fate of BD-1. The effects of BD-1 on microbial activity were investigated using 16S rRNA gene-based quantitative polymerase chain reaction (qPCR) analysis. The biodegradation of BD-1 was accompanied by a reduction in TCE, and the initially high TOC levels decreased rapidly as BD-1 was transformed into VFAs. qPCR analysis showed that the genus Dehalobacter became progressively dominant through the experiment. These results suggested that BD-1 might dechlorinate TCE by activating dechlorinating bacteria.
Assuntos
Ácidos Graxos Monoinsaturados/farmacologia , Água Subterrânea/química , Halogenação , Tricloroetileno/isolamento & purificação , Poluentes Químicos da Água/isolamento & purificação , Poluição da Água/análise , Bactérias/efeitos dos fármacos , Bactérias/genética , Materiais Biocompatíveis/farmacologia , Biodegradação Ambiental/efeitos dos fármacos , Dióxido de Carbono/análise , Eletroforese em Gel de Gradiente Desnaturante , Ácidos Graxos Monoinsaturados/química , Ácidos Graxos Voláteis/análise , Água Subterrânea/microbiologia , Halogenação/efeitos dos fármacos , Hidrogênio/análise , Japão , Dados de Sequência Molecular , Oxirredução/efeitos dos fármacos , RNA Ribossômico 16S/genética , Óleo de Brassica napusRESUMO
The effect of essential oils, such as raspberry ketone, on androgen (AR) receptor was investigated using a MDA-kb2 human breast cancer cell line for predicting potential AR activity. Among them, eugenol had the highest AR antagonistic activity with its IC(50) value of 19 microM. Raspberry ketone, which has threefold higher anti-obese activity than that of capsaicin, also had AR antagonist activity with its IC(50) value of 252 microM. Based on these findings, a more precise CoMFA model was proposed as follows: pIC(50) [log (1/IC(50))]=3.77+[CoMFA field terms] (n=39, s=0.249, r(2)=0.834, s(cv)=0.507, q(2)=0.311 (three components).
Assuntos
Antagonistas de Androgênios/farmacologia , Antagonistas de Receptores de Andrógenos , Butanonas/farmacologia , Óleos Voláteis/farmacologia , Receptores Androgênicos/metabolismo , Antagonistas de Androgênios/química , Butanonas/química , Linhagem Celular Tumoral , Genes Reporter , Humanos , Concentração Inibidora 50 , Modelos Moleculares , Óleos Voláteis/química , Ligação Proteica , Receptores Androgênicos/químicaRESUMO
The effect of 32 flavonoids on androgen (AR) and glucocorticoid receptors (GR) was investigated using an MDA-kb2 human breast cancer cell line to predict potential AR and GR activities. Among them, 5-hydroxyflavone (7) had the highest AR antagonistic activity with an IC(50) value of 0.3 microM, whereas 6-methoxyflavone (11) had the highest induced luciferase activity with an EC(150) value of 0.7 microM. Genistein (2) and daizein (1) showed a sufficient increase of luciferase activities as their concentrations increased with EC(150) values of 4.4 and 10.1 microM, respectively. These findings provide evidence of a fundamental property of their structure-activity relationship with AR and/or GR.