[Effects of Eupolyphaga Sinensis Walker on erythrocyte's CR1 activity and anti-cardiolipin antibody level in rats with stagnation of blood].

AIM: To study the effect of Eupolyphaga Sinensis Walker(ESW) on red blood cell immune adherence (RCIA) and serum anticardiolipin antibody level in rat model of Yin-deficiency Huo-excess with chronic blood stasis. METHODS: The model was made by i.m. injection of dexamethasone (0.5 mg/kg) and adrenaline (0.84 mg/kg). The serum anti-cardiolipin antibodies (ACA) ACA-IgG, ACA-IgA and ACA-IgM and the plasma D-dimmer levels were measured by using enzyme-linked immunosorbent assay (ELISA). The body and organ weight of the rats were measured. RESULTS: For rats of Yin-deficiency Huo-excess, rosette rates of red blood cell C3b receptors (RBC-C3b RR) and red blood cell cancer (RBC-CaR) decreased, the serum ACA-IgG, ACA-IgA, ACA-IgM and the plasma D-dimmer levels markedly increased, body weight and the weight of spleen and thymus all decreased. ESW (5 mg/kg, 10 mg/kg) increased RBC-C3bRR and RBC-CaR, reduced serum ACA-IgG, ACA-IgA, ACA-IgM and plasma D-dimmer levels, and increased spleen weight of the rats. CONCLUSION: ESW can boost the immune function in rats of Yin-deficiency Huo-excess with chronic blood stasis.

Expression of Wild-type and Mutant p16 in H460 Cell Line.

Two p16 mutants, P48L and D74N, were obtained by site-directed mutagenesis using two step PCR method. Mutant p16 cDNA and wild-type p16 cDNA were colned into the mammalian expression vector pcDNA3 to construct p16 expression vector pCMV-p16, pCMV-p16P48L and pCMV-p16D74N, respectively. After the introduction of these expression vectors into human lung cancer cell line H460 in which endogenous p16 gene was homozygously deleted, exogenous p16 expression was detected in G418 resistant cells by Northern blotting and immunocytochemistry staining. The results of immunofluorescence and immunocytochemistry staining showed that the P16 protein was located in the cell cytoplasm. The p16 cDNAs amplificated from the genomic DNA of recombinant H460 cell lines indicated that the plasmid p16 cDNA was integrated into the chromosome of cell lines. That the over expression of wild-type p16 caused G1 arrest suggested the wild-type P16 protein expressed in H460 cell line to be a functional protein.