RESUMO
Strain LA220T, isolated from seawater of the Eastern Pacific Ocean, was subjected to a polyphasic taxonomic study. Cells of the strain were Gram-stain-negative, aerobic, motile and short rod-shaped. On the basis of 16S rRNA gene sequence analysis, strain LA220T showed high similarity to Henriciella litoralis SD10T (98.5â%), Henriciella marina DSM 19595T (98.3â%) and Henriciellaaquimarina P38T (97.5â%), and exhibited less than 97.0â% 16S rRNA gene sequence similarity with respect to the type strains of other Hyphomonadaceae species. Phylogenetic analyses revealed that strain LA220T fell within the cluster of the genus Henriciella. The average nucleotide identity and in silico DNA-DNA hybridization values between strain LA220T and the type strains of Henriciella species were 74.8-76.8 and 18.4-20.8â%, respectively. The sole respiratory quinone was ubiquinone-10 (Q-10). The principal fatty acids were summed feature 8 (C18â:â1ω7c and/or C18â:â1ω6c) and C16â:â0. The major polar lipids were three unidentified glycolipids. The DNA G+C content was 59.9 mol%. Phylogenetic distinctiveness, chemotaxonomic differences and phenotypic properties revealed that strain LA220T could be differentiated from recognized Henriciella species. Therefore, strain LA220T is considered to represent a novel species of the genus Henriciella, for which the name Henriciella pelagia sp. nov. (type strain LA220T=CGMCC 1.15928T=KCTC 52577T) is proposed.
Assuntos
Alphaproteobacteria/classificação , Filogenia , Água do Mar/microbiologia , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Hibridização de Ácido Nucleico , Oceano Pacífico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
Strain Ery9T, isolated from surface seawater of the Atlantic Ocean, and strain Ery22T, isolated from deep-sea sediment of the Indian Ocean, were subjected to a taxonomic study using a polyphasic approach. Cells of the two strains were Gram-stain-negative, aerobic and rod-shaped. They produced yellow pigments and lacked bacteriochlorophyll a. On the basis of 16S rRNA gene sequence analysis, strain Ery9T was closely related to Croceicoccus naphthovorans PQ-2T (with 16S rRNA gene sequence similarity of 97.7 %), and strain Ery22T was closely related to Croceicoccusmarinus E4A9T (98.3 %). The 16S rRNA gene sequence similarity between strain Ery9T and strain Ery22T was 96.6 %. Phylogenetic analyses revealed that strains Ery9T and Ery22T fell within the cluster of the genus Croceicoccus and represented two independent lineages. The average nucleotide identity (ANI) values and the genome-to-genome distances between strains Ery9T and Ery22T and the type strains of species of the genus Croceicoccus with validly published names were 73.7-78.4 % and 20.1-22.3 %, respectively. The major respiratory quinone of the two isolates was ubiquinone-10 (Q-10). The DNA G+C contents of strains Ery9T and Ery22T were 62.8 and 62.5 mol%, respectively. Differential phylogenetic distinctiveness and chemotaxonomic differences, together with phenotypic properties, revealed that strains Ery9T and Ery22T could be differentiated from their closely related species. Therefore, it is concluded that strains Ery9T and Ery22T represent two novel species of the genus Croceicoccus, for which the names Croceicoccus pelagius sp. nov. (type strain Ery9T=CGMCC 1.15358T=DSM 101479T) and Croceicoccus mobilis sp. nov. (type strain Ery22T=CGMCC 1.15360T=DSM 101481T), are proposed.
Assuntos
Alphaproteobacteria/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar/microbiologia , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Oceano Atlântico , Técnicas de Tipagem Bacteriana , Bacterioclorofila A/química , Composição de Bases , DNA Bacteriano/genética , Oceano Índico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A novel bacterial strain, NH131(T), was isolated from deep-sea sediment of South China Sea. Cells were strictly aerobic, Gram-stain negative, short rod-shaped and motile with a single lateral flagellum. Strain NH131(T) grew optimally at pH 6.5-7.0 and 25-30 °C. 16S rRNA gene sequence analysis revealed that strain NH131(T) belonged to the genus Devosia, sharing the highest sequence similarity with the type strain, Devosia geojensis BD-c194(T) (96.2%). The predominant fatty acids were C(18â:â1)ω7c, 11-methyl C(18â:â1)ω7c, C(18â:â0) and C(16â:â0). Ubiquinone 10 was the predominant ubiquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, three glycolipids and two unknown lipids. The DNA G+C content of strain NH131(T) was 63.0 mol%. On the basis of the results of polyphasic identification, it is suggested that strain NH131(T) represents a novel species of the genus Devosia for which the name Devosia pacifica sp. nov. is proposed. The type strain is NH131(T) (â=âJCM 19305(T)â=âKCTC 32437(T)).
Assuntos
Sedimentos Geológicos/microbiologia , Hyphomicrobiaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hyphomicrobiaceae/genética , Hyphomicrobiaceae/isolamento & purificação , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A Gram-negative, motile, rod-shaped bacterial strain, designated S3-22(T), was isolated from a sediment sample collected from a ballast water tank of a commercial ship and subjected to a polyphasic taxonomic characterization. The isolate formed small, light-yellow, semi-translucent and circular colonies on solid complex media. The strain was oxidase- and catalase-positive and metabolized a large number of carbon sources. Chemotaxonomic analysis showed ubiquinone Q-10 as predominant respiratory quinone, phosphatidylglycerol and an unidentified glycolipid as major polar lipids and iso-C(17â:â1)ω9c, iso-C(15â:â0), C(16â:â1)ω7c and/or iso-C(15â:â0) 2-OH, C(16â:â0), iso-C(17â:â0) and C(18â:â1)ω7c as major fatty acids and the hydroxy fatty acids iso-C(17â:â0) 3-OH and C(16â:â0) 3-OH. The genomic DNA G+C content was 54.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate has 96.1â% similarity to the type strain of Kordiimonas gwangyangensis, the sole described species within the order Kordiimonadales, and less than 91.0â% similarity to other recognized species. On the basis of phenotypic and genotypic data, strain S3-22(T) represents a novel species of the genus Kordiimonas, for which the name Kordiimonas lacus sp. nov. is proposed, with the type strain S3-22(T) (=CGMCC 1.9109(T) =JCM 16261(T)). An emended description of the genus Kordiimonas is also presented.
Assuntos
Alphaproteobacteria/classificação , Filogenia , Microbiologia da Água , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Genótipo , Sedimentos Geológicos/microbiologia , Glicolipídeos/análise , Dados de Sequência Molecular , Fenótipo , Fosfatidilgliceróis/análise , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , NaviosRESUMO
A Gram-negative, neutrophilic and rod-shaped bacterium, strain ZH17(T), was isolated from a marine sediment of the East China Sea and subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-7.5â% (w/v) NaCl and at pH 6.5-9.0; optimum growth was observed with 0.5-3.0â% (w/v) NaCl and at pH 7.5. Chemotaxonomic analysis showed ubiquinone-10 as predominant respiratory quinone and C(18â:â1)ω7c, 11-methyl C(18â:â1)ω7c, C(16â:â0), C(12â:â0) 3-OH and C(16â:â0) 2-OH as major fatty acids. The genomic DNA G+C content was 63.5 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Ruegeria. Strain ZH17(T) exhibited the closest phylogenetic affinity to the type strain of Ruegeria pomeroyi, with 97.2â% sequence similarity, and less than 97â% sequence similarity with respect to other described species of the genus Ruegeria. The DNA-DNA reassociation value between strain ZH17(T) and R. pomeroyi DSM 15171(T) was 50.7â%. On the basis of phenotypic and genotypic data, strain ZH17(T) represents a novel species of the genus Ruegeria, for which the name Ruegeria marina sp. nov. (type strain ZH17(T) =CGMCC 1.9108(T) =JCM 16262(T)) is proposed.
Assuntos
Sedimentos Geológicos/microbiologia , Filogenia , Rhodobacteraceae/classificação , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/análise , Dados de Sequência Molecular , Fenótipo , RNA Ribossômico 16S/genética , Rhodobacteraceae/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análiseRESUMO
A Gram-stain-positive, halotolerant, neutrophilic, rod-shaped bacterium, strain MF38(T), was isolated from a saline-alkaline soil in China and subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-15 % (w/v) NaCl and at pH 6.5-8.5; optimum growth was observed with 3.0 % (w/v) NaCl and at pH 7.0. Chemotaxonomic analysis showed menaquinone MK-7 as the predominant respiratory quinone and anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(15 : 0), C(17 : 0) and C(16 : 0) as major fatty acids. The genomic DNA G+C content was 35.3 mol%. 16S rRNA gene sequence similarities of strain MF38(T) with type strains of described Gracilibacillus species ranged from 95.3 to 97.7 %. Strain MF38(T) exhibited the closest phylogenetic affinity to the type strain of Gracilibacillus dipsosauri, with 97.7 % 16S rRNA gene sequence similarity. The DNA-DNA reassociation between strain MF38(T) and G. dipsosauri DSM 11125(T) was 45 %. On the basis of phenotypic and genotypic data, strain MF38(T) represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus ureilyticus sp. nov. (type strain MF38(T) =CGMCC 1.7727(T) =JCM 15711(T)) is proposed.
Assuntos
Bactérias Gram-Positivas Formadoras de Endosporo/isolamento & purificação , Filogenia , Microbiologia do Solo , Composição de Bases , DNA Bacteriano/genética , Bactérias Gram-Positivas Formadoras de Endosporo/classificação , Bactérias Gram-Positivas Formadoras de Endosporo/genética , Bactérias Gram-Positivas Formadoras de Endosporo/crescimento & desenvolvimento , Dados de Sequência Molecular , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Esporos Bacterianos/classificação , Esporos Bacterianos/genéticaRESUMO
A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1g(T), was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-17.0â% (w/v) NaCl and at pH 6.0-9.0; optimum growth was observed in the presence of 3.0-5.0â% (w/v) NaCl and at pH 7.0. Strain A1g(T) was motile, formed cream-coloured colonies, was catalase- and oxidase-positive and was able to hydrolyse aesculin, Tween 40 and Tween 60. Chemotaxonomic analysis revealed menaquinone-7 as the predominant respiratory quinone and anteiso-C15:0, anteiso-C17:0, iso-C16:0 and iso-C15:0 as major fatty acids. The genomic DNA G+C content of strain A1g(T) was 36.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the new isolate belonged to the genus Oceanobacillus and exhibited closest phylogenetic affinity to the type strains of Oceanobacillus oncorhynchi subsp. incaldanensis (97.9â% similarity) and O. oncorhynchi subsp. oncorhynchi (97.5â%), but less than 97â% sequence similarity with respect to the type strains of other recognized Oceanobacillus species. Levels of DNA-DNA relatedness between strain A1g(T) and reference strains O. oncorhynchi subsp. incaldanensis DSM 16557(T), O. oncorhynchi subsp. oncorhynchi JCM 12661(T) and Oceanobacillus iheyensis DSM 14371(T) were 29, 45 and 38â%, respectively. On the basis of phenotypic and genotypic data, strain A1g(T) is considered to represent a novel species of the genus Oceanobacillus, for which the name Oceanobacillus neutriphilus sp. nov. is proposed. The type strain is A1g(T) (=CGMCC 1.7693(T) =JCM 15776(T)).
Assuntos
Bacillaceae/classificação , Bacillaceae/isolamento & purificação , Reatores Biológicos/microbiologia , Esgotos/microbiologia , Bacillaceae/genética , Bacillaceae/fisiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , Catalase/metabolismo , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Esculina/metabolismo , Ácidos Graxos/análise , Concentração de Íons de Hidrogênio , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Oxirredutases/metabolismo , Filogenia , Pigmentos Biológicos/biossíntese , Polissorbatos/metabolismo , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismoRESUMO
Two strains, CN44(T) and CN47(T), isolated from marine sediment of the East China Sea, were characterized by using a polyphasic approach. The isolates were Gram-negative, strictly aerobic, non-spore-forming rods. The chemotaxonomic characteristics of these isolates included the presence of C(18 : 1)omega7c, C(16 : 0), iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c and C(10 : 0) 3-OH as the major cellular fatty acids and Q-8 as the predominant ubiquinone. The DNA G+C contents of strains CN44(T) and CN47(T) were 62.5 and 56.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CN44(T) was related to members of the genus Marinobacterium. The most closely related described organism was the type strain of Marinobacterium rhizophilum (95.3 % sequence similarity). Strain CN47(T) showed the highest sequence similarity to the type strain of Marinobacterium stanieri (97.8 %) and <97 % similarity to other type strains of described Marinobacterium species. The level of DNA-DNA relatedness between strain CN47(T) and M. stanieri DSM 7027(T) was 46 %. On the basis of phenotypic and genotypic properties, strains CN44(T) and CN47(T) represent two novel species within the genus Marinobacterium, for which the names Marinobacterium nitratireducens sp. nov. (type strain, CN44(T) =CGMCC 1.7286(T) =JCM 15523(T)) and Marinobacterium sediminicola sp. nov. (type strain, CN47(T) =CGMCC 1.7287(T) =JCM 15524(T)) are proposed.
Assuntos
Alteromonadaceae/classificação , Sedimentos Geológicos/microbiologia , Água do Mar/microbiologia , Alteromonadaceae/genética , Alteromonadaceae/isolamento & purificação , Alteromonadaceae/fisiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/análise , DNA Ribossômico/análise , Ácidos Graxos/análise , Genes de RNAr , Genótipo , Dados de Sequência Molecular , Fenótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Three Gram-negative, aerobic, motile, halophilic, rod-shaped strains (CN46(T), CN71 and CN74(T)) were isolated from sediment of the East China Sea and subjected to a polyphasic taxonomic study. Strains CN46(T) and CN71 had identical 16S rRNA gene sequences and phenotypic characteristics. Strain CN46(T) was moderately halophilic. Growth of strain CN46(T) was observed between 0.5 and 10.0 % (w/v) NaCl (optimal growth at 3.0-5.0 %) and between pH 6.5 and 9.0. Strain CN74(T) grew over a wider range of pH (pH 6.0-9.5); the optimum NaCl concentration for growth was 1.0-3.0 %. The major fatty acids of strain CN46(T) were C(16 : 1)omega9c, C(16 : 0) and C(12 : 0), whereas strain CN74(T) contained C(16 : 0), C(16 : 1)omega9c, C(18 : 1)omega9c and C(12 : 0). The DNA G+C contents of the three isolates were between 58.0 and 58.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains CN46(T), CN71 and CN74(T) grouped together within the cluster of Marinobacter species. 16S rRNA gene sequence similarities of the three strains with the type strains of Marinobacter species ranged from 94.0 to 97.1 %. The DNA-DNA hybridization values of strain CN74(T) with strains CN46(T) and CN71 were 35.0 and 36.0 %, respectively. Levels of DNA-DNA relatedness between strains CN46(T) and CN74(T) and Marinobacter pelagius CGMCC 1.6775(T), Marinobacter gudaonensis CGMCC 1.6294(T) and Marinobacter koreensis DSM 17924(T) were 15.3-45.2 %. The results of DNA-DNA hybridizations, fatty acid analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from closely related species. Two novel species are proposed, named Marinobacter mobilis sp. nov. (type strain CN46(T) =CGMCC 1.7059(T) =JCM 15154(T)) and Marinobacter zhejiangensis sp. nov. (type strain CN74(T) =CGMCC 1.7061(T) =JCM 15156(T)).
Assuntos
Sedimentos Geológicos/microbiologia , Marinobacter/classificação , Marinobacter/fisiologia , China , Marinobacter/genética , Dados de Sequência Molecular , Oceanos e Mares , Filogenia , RNA Ribossômico 16S/genética , Especificidade da EspécieRESUMO
A halophilic, Gram-negative bacterial strain, designated AJ261T, which was isolated from a soil sample from a salt lake on the Qinghai-Tibet Plateau, was subjected to a polyphasic taxonomic study. The isolate grew optimally in the presence of 3-5 % NaCl and used various carbohydrates as sole carbon and energy sources. The genomic DNA G+C content was 63.0 mol%. The predominant fatty acids were C18 : 1omega7c, C16 : 0 and C12 : 0. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate had the highest sequence similarity with respect to type strains of Halomonas elongata (98.2 %), Halomonas eurihalina (98.1 %) and Halomonas halmophila (97.2 %). The DNA-DNA relatedness of strain AJ261T with respect to H. elongata NBRC 15536T, H. eurihalina CGMCC 1.2318T and H. halmophila DSM 5349T was 42, 25 and 26 %, respectively. Overall, the phenotypic, genotypic and phylogenetic results demonstrate that strain AJ261T represents a novel species within the genus Halomonas, for which the name Halomonas caseinilytica is proposed. The type strain is AJ261T (=CGMCC 1.6773T =JCM 14802T).