[Influenza viruses studied by fluorescent immunoassay with temporary resolution]. / Issledovanie virusov grippa metodom fliuorestsentnogo immunoanaliza s vremennym razresheniem.

Monoclonal antibodies, epidemic strains of influenza A and B viruses, conjugates were studied by fluorescence immunoassay with temporary resolution using equipment of different companies. Differences and specificity of monoclonal antibodies to influenza A and B viruses were demonstrated. The highest sensitivity of the method with the test system used was determined, being 20 ng/ml of viral protein. The method was shown to be useful for influenza virus detection both in solutions containing purified virions and in virus-containing allantoic preparations. It was established that virological studies could be carried out using conjugates and knock-down microplates of the national make alongside with foreign ones.

[Antigenic and biological characteristics of influenza virus A strains isolated in 1985]. / Antigennye i biologicheskie osobennosti shtammov virusa grippa A, izolirovannykh v 1985 g.

The antigenic structure of hemagglutinin of influenza A virus (H3N2) strains isolated in 1985 was studied using a series of monoclonal antibody to A/Dunedin/4/73/A (H3N2) and A/Bangkok/1/79/A (H3N2), and biological and physico-chemical properties of these strains were compared with those of influenza A (H3N2) virus of 1983 and reference A (H3N2) of 1979-1984 (the rate of adsorption on chick erythrocytes and eluting activity, thermostability of hemagglutinin and neuraminidase, sensitivity to nonionic detergents, sensitivity to remantadine, analysis of virion polypeptide composition). A high degree of heterogeneity of the 1985 strain population of influenza A (H3N2) virus was revealed both in the antigenic structure of hemagglutinin and in all the biological and physico-chemical parameters tested. It was suggested that the A/Caen/1/84 strain originated not from A/Philippines/2/82 but directly from A/Texas/1/77.

[Comparative characteristics of epidemic strains of the influenza virus B isolated in 1980-1981]. / Sravnitel'naia kharakteristika épidemicheskikh shtammov virusa grippa B 1980-1981 gg. vydeleniia.

The epidemic situation of 1980-1981 was conditioned by the emergence of new antigenic variants of influenza B virus with an altered composition of hemagglutinin and neuraminidase. The strains isolated in the epidemic season of 1980-1981 vary in their antigenic and biological properties and the degree of relationship with previously circulating influenza B viruses, although they show sufficient stability of molecular weights of major virion polypeptides. Influenza B viruses occurring after 1972 should be classified into a new, 5th group. The epidemic strains which were spread in 1980-1981 belong to this group although they represent already new drift variants.

[Electrophoretic mobility of polypeptides in different strains on human influenza A and B viruses]. / Elektroforetocheskaia podvizhnost' polipeptidov u raznykh shatmmov virusov grippa A i B cheloveka.

The method of electrophoresis in a single polyacrylamide gel plate was used for comparative study on virion polypeptides mobility in human influenza A and B virus strains. Molecular masses of individual polypeptides and their portion in the virion were determined. No variations in the migration speed of nucleoprotein (NP) and membrane protein (MP) were found in strains belonging to the same genus, but there were differences in the migration speed of these proteins in the genus A and genus B. Significant differences in migration and the content of glycoproteins, particularly of the heavy chain of hemagglutinin (HAI) in the genus A strain having different subtypes of hemagglutinin, including strains A/WS/33 (HONI), A/FM/1/47 (HINI), A/Sing/1/57 (H2N2), A/Port Chalmers/73 (H3N2), as well as in the genus B in strains B/Lee/40, B/Yamagata/1/73, and B/Johanesbourg/56. No differences in the mobility of glycoproteins in strains similar in the antigenic specificity of hemagglutinin and neuraminidase were found. On the basis of the comparative analysis of virion polypeptide electrophoregrams, three new strains on influenza A virus isolated in December, 1977, were identified as belonging to the species A (HINI).