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1.
J Hand Surg Eur Vol ; 42(3): 266-270, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27803378

RESUMO

The purpose of this study was to measure the volar cortical angles of the commercially available volar locking plates and evaluate how well they fit the distal radius. We measured the volar cortical angles of the radial and intermediate columns of eight volar locking plates and compared them with the volar cortical angles of 90 cadaver distal radii. The mean radial and intermediate column volar cortical angles of the wider plates were significantly larger than those of narrower plates. The mean radial and intermediate column volar cortical angles were significantly larger in wider radii than narrower radii. The width of the distal radius did not correlate well with the mean volar cortical angles of the radial and intermediate columns. LEVEL OF EVIDENCE: IV.


Assuntos
Placas Ósseas , Fixação Interna de Fraturas/instrumentação , Placa Palmar/cirurgia , Fraturas do Rádio/cirurgia , Idoso , Idoso de 80 Anos ou mais , Cadáver , Desenho de Equipamento , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
2.
Neurology ; 51(2): 625-7, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9710055

RESUMO

A patient with left thalamo-mesencephalic infarction presented with micrographia in the right hand as the only motor sign. Brain MRI and 99mTc ethyl cysteinate dimer (ECD) perfusion SPECT revealed ischemic lesions in the left midbrain and the anterior thalamus, but not in the basal ganglia, whereas [123I]-IPT SPECT demonstrated decreased activity of the [123I]-IPT in the left striatum. The patient's micrographia may be related to a dysfunctional nigrostriatal dopaminergic system secondary to ischemic damage to the substantia nigra.


Assuntos
Infarto Cerebral/fisiopatologia , Corpo Estriado/fisiopatologia , Dopamina/fisiologia , Escrita Manual , Mesencéfalo/irrigação sanguínea , Tálamo/irrigação sanguínea , Humanos , Masculino , Pessoa de Meia-Idade
3.
J Korean Med Sci ; 13(3): 234-40, 1998 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9681800

RESUMO

Creutzfeldt-Jakob disease (CJD), a relatively uncommon human dementia, is caused by an unconventional slow infectious agent. Several cases of CJD, clinically or histopathologically diagnosed, have been reported in Korea. In order to confirm the diagnosis of CJD and also differential diagnosis of sporadic and familial types of CJD in Korea, we studied two patients who had symptoms of CJD. The histopathological and immunohistochemical studies showed spongiform neurodegeneration and expression of abnormal isoform of prion protein (PrPSc) in astrocytes. Thus, these two patients were diagnosed CJD. To investigate whether these patients were sporadic or familial type of CJD, the molecular analyses of the prion protein gene (PRNP) were done by restriction fragment length polymorphism (RFLP) and DNA sequencing. In the cases of a healthy Korean and two CJD patients, no point mutation was detected in the known hot spots (178, 180, 200, 210, and 232) and they exhibited wild type PRNP sequences. We concluded that both patients have a sporadic type of CJD, but not familial type.


Assuntos
Síndrome de Creutzfeldt-Jakob/genética , Príons/genética , Sequência de Bases , Biópsia , Encéfalo/patologia , Síndrome de Creutzfeldt-Jakob/diagnóstico , Síndrome de Creutzfeldt-Jakob/patologia , DNA/análise , DNA/genética , Humanos , Imuno-Histoquímica , Dados de Sequência Molecular , Fases de Leitura Aberta , Polimorfismo de Fragmento de Restrição
4.
Blood ; 77(2): 387-92, 1991 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-1824679

RESUMO

Metabolic development was examined in erythroid precursor cells, which were isolated from the spleens of mice infected with the anemia-inducing strain of Friend virus (FVA cells). FVA cells undergo differentiation in vitro from the proerythroblast stage through the reticulocyte stage over a 48-hour period in the presence of erythropoietin. Concomitant with marked decreases in cellular size and energy demand, metabolic capacities of both glycolysis and oxygen consumption diminish after 48 hours in culture by 7- and 18-fold, respectively. Because the oxidative capacity decreases more than glycolytic ability does, the metabolic machinery increasingly shifts toward anaerobic metabolism. During the 48-hour period of differentiation, the 2,3-diphosphoglyceric acid (DPG) content per cell and 2,3-DPG mutase activity per cell increased eightfold and threefold, respectively. Freshly harvested FVA cells have adenosine triphosphate (ATP) levels of 7.23 +/- 2.52 mumol/10(10) cells or 3.76 +/- 1.31 mumol/mL cell water which are 12- or 2.3-fold higher, respectively, than the ATP levels of mature red blood cells. In the course of FVA cell differentiation, ATP content per cell decreases by fourfold, but ATP concentration in cell water remains unchanged because of a corresponding decrease in cellular size and water content during differentiation. These studies show that in the face of dramatic decreases in cell size and cellular energy demand, terminally differentiating erythroid cells maintain a constant ATP level by undergoing an involution of their glycolytic machinery as well as by losing their aerobic metabolic capacity.


Assuntos
Eritroblastos/metabolismo , Células Precursoras Eritroides/metabolismo , Eritropoese , Eritropoetina/farmacologia , 2,3-Difosfoglicerato , Trifosfato de Adenosina/metabolismo , Aerobiose , Anaerobiose , Animais , Bisfosfoglicerato Mutase/metabolismo , Diferenciação Celular/efeitos dos fármacos , Ácidos Difosfoglicéricos/metabolismo , Glucose/metabolismo , Glicólise , Lactatos/metabolismo , Camundongos , Consumo de Oxigênio
5.
Endocrinology ; 126(2): 1183-90, 1990 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2153521

RESUMO

The occurrence and properties of PTH-related peptide (PTH-RP) in milk was investigated. PTH-RP was purified to homogeneity from human and bovine milk using heat and acid to precipitate milk proteins followed by ion exchange chromatography and reverse-phase HPLC. The peak of PTH-RP from HPLC was detected using a sensitive bone cell bioassay. A single band of peptide was detected on silver-stained polyacrylamide gels, which migrated as a 20-21-kDa macromolecule. PTH-RP isolated from either human or bovine milk had similar electrophoretic mobilities on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The partially purified bovine PTH-RP stimulated cAMP production in UMR106-01 and OK cell lines and elicited a concentration-dependent inhibition of sodium-dependent phosphate transport in OK cells. Incubation of milk extracts with an anti-PTH antiserum did not affect their bioactivity, whereas an antihuman PTH-RP 1-34 antiserum markedly reduced the cAMP response of UMR106-01 cells to the immunoabsorbed milk extracts. A PTH antagonist, norleu PTH 3-34, blocked the stimulation of cAMP production in UMR106-01 cells treated with milk extracts. PTH-RP immunoreactivity and bioactivity occurred in milk extracts of diverse animals from both eutherian and metatherian (marsupial) species. Porcine colostrum also had immunoreactive PTH-RP, although the levels were lower than the immunoreactive PTH-RP concentrations observed in milk samples collected at 7 and 14 days of lactation. Thus, a 20-21-KDa PTH-RP is secreted into milk where it could play a role in the development of suckling, newborn animals.


Assuntos
Leite Humano/análise , Leite/análise , Proteínas/isolamento & purificação , Adenilil Ciclases/metabolismo , Animais , Bioensaio , Western Blotting , Bovinos , Cromatografia Líquida de Alta Pressão , Colostro/análise , AMP Cíclico/biossíntese , Ativação Enzimática/efeitos dos fármacos , Feminino , Cabras , Humanos , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Proteína Relacionada ao Hormônio Paratireóideo , Fosfatos/metabolismo , Proteínas/farmacologia , Suínos , Células Tumorais Cultivadas
6.
Am J Physiol ; 256(4 Pt 1): C772-8, 1989 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2539726

RESUMO

Activation of a Cl-dependent K flux by adenosine 3',5'-cyclic monophosphate (cAMP) was characterized in pig red cells, a cell type that lacks both the Ca-activated K channel and the Na-K-Cl cotransport pathway. As in other red cells, both Cl-dependent K efflux and K influx are stimulated on cell swelling. Although pig red cells fail to respond to beta-adrenergic stimuli, it is possible to raise the intracellular cAMP content by preincubating cells in the presence of 1 mM cAMP. The Cl-dependent K flux was compared in cells having a basal cAMP content of approximately 0.29 nmol/g hemoglobin vs. cAMP-loaded cells having approximately 8.4 nmol cAMP/g hemoglobin. Loading with cAMP stimulated both Cl-dependent K efflux and influx of hypotonically swollen cells. In maximally swollen cells whose volume was increased by approximately 17%, the Cl-dependent Rb influx occurs with a maximum velocity (Vmax) of 17.9 +/- 3.2 mumol.g hemoglobin (Hb)-1.h-1 and Km for Rb of 22.9 +/- 4.1 mM. In cAMP-loaded cells, both Vmax and Km were increased to 59.8 +/- 8.5 mumol.g Hb-1.h-1 and 63.1 +/- 8.8 mM, respectively. The Cl-dependent Rb influx is much larger in young cells than in old cells. However, both cell types respond to cAMP activation. Whereas cAMP and its analogues, 8-bromoadenosine 3',5'-cyclic monophosphate and dibutyryl adenosine 3',5'-cyclic monophosphate are stimulatory, AMP and guanosine 3',5'-cyclic monophosphate (cGMP) are not. These findings suggest that, like other ion transport systems, the Cl-dependent K flux of pig red cells is endowed with the capacity to respond to cAMP.


Assuntos
Cloretos/farmacologia , AMP Cíclico/farmacologia , Eritrócitos/metabolismo , Potássio/sangue , Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico , Ácido 4-Acetamido-4'-isotiocianatostilbeno-2,2'-dissulfônico/análogos & derivados , Ácido 4-Acetamido-4'-isotiocianatostilbeno-2,2'-dissulfônico/farmacologia , 8-Bromo Monofosfato de Adenosina Cíclica/farmacologia , Animais , Transporte Biológico/efeitos dos fármacos , Bucladesina/farmacologia , Bumetanida/farmacologia , Cálcio/sangue , Proteínas de Transporte/sangue , AMP Cíclico/sangue , Eritrócitos/efeitos dos fármacos , Etilmaleimida/farmacologia , Furosemida/farmacologia , Cinética , Ouabaína/farmacologia , Rubídio/sangue , Simportadores de Cloreto de Sódio-Potássio , Suínos , Acetato de Tetradecanoilforbol/farmacologia
7.
Prog Clin Biol Res ; 319: 491-502; discussion 503-4, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2622926

RESUMO

1. Metabolic development was investigated in splenic erythroblasts isolated from Friend virus infected mice and maintained in culture with erythropoietin. 2. We found that immature erythroid cells not only possess a highly oxidative metabolism, but also glycolytic activity, both of which decrease drastically in the course of maturation. 3. Most purine and pyrimidine compounds which are found in immature erythroid cells are still present in mature erythrocytes. 4. While total adenine nucleotide levels fall, the 2,3-DPG pool increases during erythroid development.


Assuntos
Envelhecimento Eritrocítico/fisiologia , Células Precursoras Eritroides/metabolismo , Eritropoetina/fisiologia , Animais , Linhagem Celular Transformada , Vírus da Leucemia Murina de Friend , Glicólise/fisiologia , Lactatos/sangue , Camundongos , Camundongos Endogâmicos , Fosfatos/sangue
8.
Biochem Biophys Res Commun ; 151(1): 370-81, 1988 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-3348782

RESUMO

In order to directly compare the structural characteristics of renal glomerular and tubular insulin receptors, the purified isolated nephron subunits were extracted with 1% Triton X-102, fractionated by DEAE-Sephacel ion exchange column chromatography and the fractions containing insulin binding proteins were identified by the precipitation of 125I-insulin-protein complexes with polyethylene glycol (PEG). The fractions containing insulin binding proteins were pooled, incubated with 125I-insulin and covalently cross-linked with disuccinimidyl suberate, followed by chromatography of the cross-linked samples on Sepharose CL-6B. From both glomeruli and tubules, three 125I-insulin-binding complexes with molecular weights of 560 KDa, 220 KDa and 95 KDa were found. SDS-PAGE of these complexes from glomeruli and tubules under both reducing and nonreducing conditions gave similar patterns of 125I-insulin-crosslinked components, with the exception of the polypeptide pattern from the 560 KDa peak fraction which was markedly different between glomeruli and tubules with the former giving major labeled components at 170 and 68 KDa while the latter showed labeled components of 125 KDa and greater than 250 KDa. Glomerular and tubular insulin receptors, therefore, display similar subunit composition under reducing conditions, but differ in the non-reduced state, suggesting that these complexes may differ in the extent and/or nature of disulfide bonding.


Assuntos
Glomérulos Renais/análise , Túbulos Renais/análise , Receptor de Insulina/isolamento & purificação , Animais , Autorradiografia , Membrana Celular/metabolismo , Cromatografia em Gel , Cromatografia por Troca Iônica , Eletroforese em Gel de Poliacrilamida , Glomérulos Renais/ultraestrutura , Túbulos Renais/ultraestrutura , Peso Molecular , Ratos , Receptor de Insulina/análise
9.
J Mol Cell Cardiol ; 18(2): 157-68, 1986 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-3514924

RESUMO

Insulin binding to isolated cardiac myocytes from normal and streptozotocin-induced diabetic rats was investigated. We found that at high affinity sites, the maximum numbers of insulin binding sites per cell are 33 000 and 22 000 for normal and diabetic myocytes, respectively with no discernible difference in receptor affinity. However, since the yield of myocytes from the diabetic heart was only 1/3 of the normal heart, it is suspected that the insulin function in the diabetic heart may be significantly lower than that in the normal heart. Chloroquine was found to markedly decrease insulin degradation with concomitant increase in net insulin uptake by isolated myocytes. This suggests that insulin degradation may take place within lysosomes after insulin is internalized. To determine whether internalization of insulin in myocytes is an energy dependent process, insulin binding and subsequent degradation were assessed in cells depleted of ATP by treatment with various metabolic inhibitors (2,4-dinitrophenol, NaF and iodoacetic acid). Depletion of the cellular ATP level resulted in a decrease in both insulin uptake and degradation. In diabetic myocytes, the general relationship between cellular ATP level and insulin uptake and degradation was similar to that found in normal myocytes. However, in diabetic myocytes, the cellular ATP level and insulin uptake were lower, but insulin degradation was greater than in normal myocytes. Insulin uptake by normal and ATP depleted cells at 4 degrees C (16 h) was lower than at 37 degrees C (1 h), while the ATP level was almost the same at both temperatures. This suggests that the internalization of insulin is a temperature as well as an ATP dependent process.


Assuntos
Diabetes Mellitus Experimental/metabolismo , Insulina/metabolismo , Miocárdio/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Sítios de Ligação/efeitos dos fármacos , Separação Celular , Cloroquina/farmacologia , Diabetes Mellitus Experimental/patologia , Dinitrofenóis/farmacologia , Glucose/farmacologia , Iodoacetatos/farmacologia , Ácido Iodoacético , Masculino , Miocárdio/patologia , Ratos , Ratos Endogâmicos , Fluoreto de Sódio/farmacologia , Temperatura , Fatores de Tempo
10.
Biochim Biophys Acta ; 601(3): 640-53, 1980 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-7417442

RESUMO

Alanine chloromethylketone and leucine chloromethylketone were synthesized and their effects on amino acid transport in sarcoma 37 mirone ascites tumor (S37) cells were studied. Alanine chloromethylketone preincubation weakly inhibited system A. Leucine chloromethylketone preincubation strongly inhibited both amino acid transport systems L and A. Leucine chloromethylketone was also a competitive inhibitor of leucine transport. Labeled leucine chloromethylketone was concentrated by S37 cells. Leucine chloromethylketone preincubation inhibition was concentration dependent and partial protection of transport was afforded by leucine. Steady-state retention of amino acids was decreased more than the initial velocity of transport by leucine chloromethylketone preincubation. Glutathione was also depleted. Labeled leucine chloromethylketone was incorporated in a plasma membrane protein fraction comigrating on a DEAE-cellulose column (DE52) with gamma-glutamyltranspeptidase activity. There was a modest increase in vital staining after treatment of S37 cells with leucine chloromethylketone, and glucose uptake was also inhibited. Whilst several effects occur during treatment of S37 cells with leucine chloromethylketone, it is suggested than one prominent effect is alkylation of amino acid transport system components.


Assuntos
Clorometilcetonas de Aminoácidos/farmacologia , Aminoácidos/metabolismo , Sarcoma 37/metabolismo , Alanina/metabolismo , Animais , Transporte Biológico/efeitos dos fármacos , Membrana Celular/metabolismo , Glucose/metabolismo , Glutationa/metabolismo , Leucina/metabolismo , Camundongos
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