The role of egg drop syndrome virus in acute respiratory disease of goslings.

An outbreak of severe acute respiratory disease characterized by tracheitis and bronchitis was observed in young goslings on a large-scale goose farm in Hungary. Histological examination revealed amphophilic intranuclear inclusion bodies in the superficial epithelial cells of the trachea and bronchi. Adenovirus-like particles were detected by electron microscopy, and the virus isolated from the trachea and the lungs was identified as egg drop syndrome (EDS) virus by serological and genomic examination. The clinical and pathological signs were reproduced by intratracheal administration of the virus isolate to 1-day-old goslings free of EDS antibodies. The presence of EDS virus DNA in different organs of the naturally and experimentally infected goslings was detected by polymerase chain reaction. This is the first report on the involvement of EDS virus in severe respiratory disease of geese.

Additional data on Mycoplasma disease of goslings.

Epidemiological, pathomorphological, histological and microbiological studies on 272 gosling from 51 flocks showed that disease occurred generally at 3 to 4 weeks of age with morbidity of 15 to 25% and mortality of 3 to 8%. It was characterized by respiratory and nervous symptoms accompanied by serous-fibrinous inflammation of the mucosal membrane of the respiratory tract, pericardium and cerebral membranes. Mycoplasmas of 1220 type were isolated from trachea and airsac walls of 50% of the samples tested, but no adeno or reoviruses could be detected in homogenates of trachea and airsac wall, and no serological response indicative of Derzsy's disease could be demonstrated. E. coli, Ps. aeruginosa, S. typhimurium and Sir. pyogenes were occasionally cultured from the heart and liver of goslings. Cloacal and oviduct swabs and phallus lympha samples from five parent goose flocks also frequently revealed 1220 type mycoplasmas.

Demonstration of Clostridium septicum infection in a goose flock.

Clostridium septicum infection causing 5.0 to 5.2% mortality is reported for the first time in the literature from six-week-old growing geese in three flocks comprising 5,200, 5,500 and 5,900 geese, respectively. The affected birds exhibited weakness, uncoordinated movement, ataxia and, frequently, oblique position of the head and neck (torticollis) as well as signs indicative of dysequilibrium. The affected birds died within 18-24 h. Gross pathological examination revealed anaemia, hepatitis with map-like necroses of irregular outline (Fig. 1), acute enteritis, pulmonary oedema and cardiac dilatation. Light and electron-microscopic examination showed that the sinusoids of the liver were markedly dilated (Fig. 2) and filled with serous exudate and gas (Figs 2 and 3), and the hepatocytes surrounding them exhibited severe oedema (Fig. 4). Among the hepatocytes, ciliated bacteria 7-10 mu in length and 1-3 mu in width, bounded by a well-defined cell wall and often showing signs of spore formation were observed (Figs 5 and 6). By bacteriological examination the pathogen was isolated, its properties were studied, and the clinical entity of malignant oedema was experimentally reproduced by intramuscular injection of guinea-pigs and rabbits. The applied antibiotic (oxytetracycline) and furazolidone therapy proved effective.