RESUMO
Bla g 2 is one of the most potent cockroach allergens. No effective treatment or vaccination strategies are yet available. We evaluated the prophylactic efficacy of Bla g 2 DNA vaccination in a mouse model of allergic airway inflammation. C57/BL6 mice were given Bla g 2 DNA vaccine prior to sensitization with recombinant Bla g 2 (rBla g 2) antigens, followed by nebulized rBla g 2 challenge. Bla g 2 vaccine could express at both transcriptional and translational levels in mammalian cells. Moreover, Bla g 2 vaccine significantly reduced the total inflammatory cell infiltrate and eosinophilia in bronchoalveolar lavage fluid, and markedly decreased allergen-induced inflammatory infiltrates in the lungs and Bla g 2-specific IgE in serum upon challenge with rBla g 2. Importantly, Bla g 2 vaccine could induce the production of antigen-specific IFN-γ and downregulated Th2 pro-inflammatory cytokines IL-4, IL-5, and IL-13. Thus, DNA vaccination showed protective efficacy against a clinically relevant allergen, Bla g 2.
Assuntos
Ácido Aspártico Endopeptidases/imunologia , Baratas/imunologia , Eosinófilos/efeitos dos fármacos , Hipersensibilidade/prevenção & controle , Vacinas de DNA/imunologia , Animais , Ácido Aspártico Endopeptidases/administração & dosagem , Líquido da Lavagem Broncoalveolar/citologia , Líquido da Lavagem Broncoalveolar/imunologia , Linhagem Celular Tumoral , Eosinófilos/imunologia , Eosinófilos/patologia , Feminino , Hipersensibilidade/imunologia , Hipersensibilidade/patologia , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Interferon gama/sangue , Interferon gama/imunologia , Interleucina-13/sangue , Interleucina-13/imunologia , Interleucina-4/sangue , Interleucina-4/imunologia , Interleucina-5/sangue , Interleucina-5/imunologia , Contagem de Leucócitos , Camundongos , Transfecção , Vacinação , Vacinas de DNA/administração & dosagemRESUMO
Premenstrual asthma is quite common, affecting up to 40% of women. Some studies suggest exacerbations of asthma requiring emergency department visits or hospitalizations are more common in the perimenstrual phase. Several drug treatment modalities for premenstrual asthma have been evaluated. Drug therapies that may be beneficial include leukotriene receptor antagonists, long acting inhaled beta2 agonists, progesterone, estradiol, and gonadotropin-releasing hormone (GnRH) analogs. Further double-blind, randomized, placebo-controlled studies are required before the optimal drug therapy for premenstrual asthma is established.