RESUMO
Two new monoterpene esters illigerates F and G (1 and 2) together with 5 know compounds illigerate A (3), illigerate C (4), actinodaphnine (5), N-methylactinodaphnine(6) and N-methyllaurotetanine(7) were isolated from Illigera aromatica S. Z. Huang et S. L. Mo. Their structures were identified by extensive NMR data and by comparing with the known compounds. The anti-inflammatory activity of four monoterpenes (1 - 4) was evaluated by inhibiting nitric oxide (NO) production in lipopolysaccharide-activated murine macrophage RAW 264.7 cells and four monoterpenoids exhibited inhibitory effect with IC50 values of 71.5 ± 7.3, 74.7 ± 5.6, 48.0 ± 7.4 and 65.1 ± 3.7 µM, respectively.
Assuntos
Anti-Inflamatórios/farmacologia , Ésteres/farmacologia , Hernandiaceae/química , Monoterpenos/farmacologia , Caules de Planta/química , Animais , Anti-Inflamatórios/química , Aporfinas/química , Aporfinas/isolamento & purificação , Aporfinas/farmacologia , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Monoterpenos/química , Óxido Nítrico/biossíntese , Células RAW 264.7RESUMO
Nine oxygenated cyclohexanoids, speciosins L-T (1-9) as well as a 5H-furan-2-one metabolite, 5'-O-acetylaporpinone A (10), together with known analogs, speciosins A, B, D, E, F, I and K (11-17), and aporpinone A (18), were isolated from a scale-up cultures of the basidiomycete Hexagonia speciosa. Their structures were elucidated by analysis of spectroscopic data, including 1D and 2D NMR. Speciosin B (12) showed significant cytotoxicity against several tumor cell lines with IC50 values in the range 0.23-3.30 µM.
Assuntos
Antineoplásicos/química , Basidiomycota/química , Cicloexanóis/química , Cicloexanonas/química , Hidrocarbonetos Cíclicos/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Cicloexanóis/isolamento & purificação , Cicloexanonas/isolamento & purificação , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Hidrocarbonetos Cíclicos/isolamento & purificação , Concentração Inibidora 50 , Estrutura Molecular , PrenilaçãoRESUMO
A new isoprenyl phenyl ether riboside, 3-(3-methylbut-2-enyloxy)-4-O-alpha-D-ribofuranose benzoic acid methyl ester (1), was isolated from the culture of basidiomycete Laccaria amethystea. The structure of 1 was elucidated on the basis of extensive spectroscopic analysis.