Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Contraception ; 84(6): 642-8, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22078196

RESUMO

BACKGROUND: This study was conducted to observe the in vivo effect of 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) on embryo implantation in rats and its in vitro effect on cell adhesion. STUDY DESIGN: The anti-implantation efficacy of AEBSF in rats was determined by counting the number of visible implanted embryos on day 8 of pregnancy following intrauterine (5 mg and 10 mg AEBSF per horn) or tail vein (10 mg AEBSF per rat) administration on day 3 of pregnancy. The effects of AEBSF on cell adhesion were detected, respectively, by using the mouse blastocysts-endometrial cells or the human umbilical vein endothelial cells (HUVECs)-HeLa cells co-culture model. The alteration in protein secretion pattern of HUVECs and HeLa cells was detected by the proteome analysis. RESULTS: 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride showed an in vivo inhibitory effect on embryo implantation in rat. In vitro, AEBSF could disturb the growth of blastocysts on endometrial cells and inhibit the adhesion of HeLa cells on HUVECs. The treatment of AEBSF could alter the protein secretion pattern of co-cultured HUVEC-HeLa cells. CONCLUSION: 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride might be a potential leading compound for novel contraceptives, and its inhibitory effect on implantation might result from the interference in extracellular matrix remodeling process.


Assuntos
Implantação do Embrião/efeitos dos fármacos , Inibidores de Serina Proteinase/farmacologia , Sulfonas/farmacologia , Administração Intravaginal , Animais , Blastocisto/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Células Cultivadas , Técnicas de Cocultura , Anticoncepcionais Sintéticos Pós-Coito/administração & dosagem , Anticoncepcionais Sintéticos Pós-Coito/farmacologia , Relação Dose-Resposta a Droga , Endométrio/citologia , Endométrio/efeitos dos fármacos , Feminino , Células HeLa , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Humanos , Injeções Intravenosas , Camundongos , Camundongos Endogâmicos ICR , Gravidez , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Inibidores de Serina Proteinase/administração & dosagem , Sulfonas/administração & dosagem
2.
Histochem Cell Biol ; 136(2): 205-15, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21678139

RESUMO

To investigate the biological significance of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) involvement in oocyte maturation, we screened for proteins that bound to UCH-L1 in mouse ovaries, and we found that the prostate tumor overexpressed-1 (PTOV1) protein was able to bind to UCH-L1. PTOV1 is highly expressed in prostate cancers and considered as a potential marker for carcinogenesis and the progress of prostate cancer. It was reported that PTOV1 plays an important role in cell cycle regulation, but its role in mammalian oocyte development and meiosis is still unclear. In this paper, it was found that the expression levels of PTOV1 in mouse ovaries progressively increased from prepubescence to adulthood. And we found by immunohistochemistry that PTOV1 spreaded in both the cytoplasm and nuclei of oocytes during prepuberty, but in normal adult mouse oocytes, it concentrated not only in nuclei but also on the plasma membrane, though in some oocytes with abnormal shapes, PTOV1 did not display the typical distribution patterns. In granulosa cells, however, it was found to locate in the cytoplasm at all the selected ages. In postnatal mouse ovaries (28 days), estradiol treatment induced the adult-specific distribution pattern of PTOV1 in oocytes. In addition, UCH-L1 was shown to be associated with CDK1, which participated in the regulation of cell cycle and oocyte maturation. Therefore, we propose that the distribution changes of PTOV1 are age-dependent, and significant for mouse oocyte development and maturation. Moreover, the discovery that PTOV1 is associated with UCH-L1 in mouse oocytes supports the explanations for that UCH-L1 is involved in oocyte development and maturation, especially under the regulation of estrogen.


Assuntos
Envelhecimento/fisiologia , Biomarcadores Tumorais/metabolismo , Estrogênios/metabolismo , Proteínas de Neoplasias/metabolismo , Oócitos/crescimento & desenvolvimento , Oócitos/metabolismo , Oogênese/fisiologia , Ubiquitina Tiolesterase/metabolismo , Animais , Biomarcadores Tumorais/química , Proteína Quinase CDC2/química , Proteína Quinase CDC2/metabolismo , Membrana Celular/metabolismo , Estradiol/química , Estradiol/metabolismo , Estrogênios/química , Feminino , Humanos , Imuno-Histoquímica , Masculino , Camundongos , Proteínas de Neoplasias/química , Ovário/metabolismo , Distribuição Tecidual , Ubiquitina Tiolesterase/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA