RESUMO
After brain injury, neuroblasts generated from endogenous neural stem cells migrate toward the injured site using blood vessels as a scaffold, raising the possibility of reconstructing blood vessel network scaffolds as a strategy for promoting endogenous neuronal regeneration. In this study, we designed biomaterials based on the components and morphology of blood vessel scaffolds, and examined their ability to guide the migration of neuroblasts into a brain lesion site in mice. Transplanted porous sponge containing components of the basement membrane (BM) matrix enhanced neuroblast migration into the lesion, and detailed morphological examination suggested that the infiltrating cells used the BM sponge as a migration scaffold. Laminin (LN)-rich porous sponge also enhanced the migration of neuroblasts into the lesion, whereas BM gel and gelatin porous sponge did not. We conclude that the transplantation of LN-rich porous sponge promotes neuroblast migration into cortical lesions. This study highlights the possibility of using artificial blood vessel scaffolds to promote the regeneration of injured cerebral cortex.
Assuntos
Movimento Celular/efeitos dos fármacos , Córtex Cerebral/patologia , Laminina/farmacologia , Neurônios/citologia , Alicerces Teciduais/química , Animais , Astrócitos/citologia , Astrócitos/efeitos dos fármacos , Membrana Basal/química , Camundongos Endogâmicos ICR , Microglia/citologia , Microglia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Poríferos , PorosidadeRESUMO
BACKGROUND: The aim of the present study was to describe the neonatal magnetic resonance imaging (MRI) findings of preterm infants with periventricular leukomalacia and mild neurological disability. METHODS: MRI findings at term equivalent were retrospectively investigated in eight preterm infants with mild disability and periventricular leukomalacia diagnosed on MRI in infancy. RESULTS: Linear, spotted, or macular areas of hyperintensity on T1-weighted imaging and hypointensity on T2-weighted imaging were identified in all subjects in the white matter lateral to the body of the lateral ventricle. No cystic lesions were seen. These findings were more widespread and more clearly visualized on T2-weighted imaging than T1-weighted imaging. CONCLUSIONS: Linear, spotted, or macular lesions that are hyperintense on T1-weighted imaging and hypointense on T2-weighted imaging are possibly compatible with periventricular leukomalacia.