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1.
Int J Oral Sci ; 7(1): 42-8, 2015 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-25572920

RESUMO

Retention wires permanently bonded to the anterior teeth are used after orthodontic treatment to prevent the teeth from relapsing to pre-treatment positions. A disadvantage of bonded retainers is biofilm accumulation on the wires, which produces a higher incidence of gingival recession, increased pocket depth and bleeding on probing. This study compares in vivo biofilm formation on single-strand and multi-strand retention wires with different oral health-care regimens. Two-centimetre wires were placed in brackets that were bonded to the buccal side of the first molars and second premolars in the upper arches of 22 volunteers. Volunteers used a selected toothpaste with or without the additional use of a mouthrinse containing essential oils. Brushing was performed manually. Regimens were maintained for 1 week, after which the wires were removed and the oral biofilm was collected to quantify the number of organisms and their viability, determine the microbial composition and visualize the bacteria by electron microscopy. A 6-week washout period was employed between regimens. Biofilm formation was reduced on single-strand wires compared with multi-strand wires; bacteria were observed to adhere between the strands. The use of antibacterial toothpastes marginally reduced the amount of biofilm on both wire types, but significantly reduced the viability of the biofilm organisms. Additional use of the mouthrinse did not result in significant changes in biofilm amount or viability. However, major shifts in biofilm composition were induced by combining a stannous fluoride- or triclosan-containing toothpaste with the mouthrinse. These shifts can be tentatively attributed to small changes in bacterial cell surface hydrophobicity after the adsorption of the toothpaste components, which stimulate bacterial adhesion to the hydrophobic oil, as illustrated for a Streptococcus mutans strain.


Assuntos
Biofilmes , Higiene Bucal , Contenções Ortodônticas , Aço Inoxidável , Sequência de Bases , Primers do DNA , Eletroforese em Gel de Poliacrilamida , Humanos , Reação em Cadeia da Polimerase
2.
Clin Oral Investig ; 17(4): 1209-18, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-22855266

RESUMO

OBJECTIVE: Bonded retainers are used in orthodontics to maintain treatment result. Retention wires are prone to biofilm formation and cause gingival recession, bleeding on probing and increased pocket depths near bonded retainers. In this study, we compare in vitro and in vivo biofilm formation on different wires used for bonded retainers and the susceptibility of in vitro biofilms to oral antimicrobials. MATERIALS AND METHODS: Orthodontic wires were exposed to saliva, and in vitro biofilm formation was evaluated using plate counting and live/dead staining, together with effects of exposure to toothpaste slurry alone or followed by antimicrobial mouthrinse application. Wires were also placed intra-orally for 72 h in human volunteers and undisturbed biofilm formation was compared by plate counting and live/dead staining, as well as by denaturing gradient gel electrophoresis for compositional differences in biofilms. RESULTS: Single-strand wires attracted only slightly less biofilm in vitro than multi-strand wires. Biofilms on stainless steel single-strand wires however, were much more susceptible to antimicrobials from toothpaste slurries and mouthrinses than on single-strand gold wires and biofilms on multi-strand wires. Also, in vivo significantly less biofilm was found on single-strand than on multi-strand wires. Microbial composition of biofilms was more dependent on the volunteer involved than on wire type. CONCLUSIONS: Biofilms on single-strand stainless steel wires attract less biofilm in vitro and are more susceptible to antimicrobials than on multi-strand wires. Also in vivo, single-strand wires attract less biofilm than multi-strand ones. CLINICAL SIGNIFICANCE: Use of single-strand wires is preferred over multi-strand wires, not because they attract less biofilm, but because biofilms on single-strand wires are not protected against antimicrobials as in crevices and niches as on multi-strand wires.


Assuntos
Anti-Infecciosos Locais/farmacologia , Biofilmes/efeitos dos fármacos , Ligas Dentárias , Desinfetantes de Equipamento Odontológico/farmacologia , Contenções Ortodônticas , Fios Ortodônticos/microbiologia , Análise de Variância , Anti-Infecciosos Locais/química , Desinfetantes de Equipamento Odontológico/química , Placa Dentária/tratamento farmacológico , Placa Dentária/microbiologia , Combinação de Medicamentos , Eletroforese em Gel Bidimensional , Feminino , Ligas de Ouro , Humanos , Masculino , Antissépticos Bucais/química , Antissépticos Bucais/farmacologia , Contenções Ortodônticas/microbiologia , Salicilatos/farmacologia , Saliva/microbiologia , Dodecilsulfato de Sódio/farmacologia , Fluoreto de Sódio/farmacologia , Aço Inoxidável , Estatísticas não Paramétricas , Terpenos/farmacologia , Cremes Dentais/química , Cremes Dentais/farmacologia
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