RESUMO
BACKGROUND: This meta-analysis aims to systematically evaluate the evidence for mindfulness-based stress reduction (MBSR) in cancer related fatigue (CRF). MATERIAL AND METHODS: In October 2018, PubMed, Embase, Cochrane Library, Clinical Trials, China Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI) and China Science Periodical Database (CSPD) were searched for randomized controlled trials on MBSR in CRF patients. Literature screening and data extraction were conducted by two reviewers. Methodological quality evaluation was assessed by the Cochrane risk of bias tool. Revman 5.3.0 performs data analysis. The trial sequential analysis software estimated the required information size for each outcome indicator. RESULTS: There have been 5 studies included in this research for meta-analysis, 356 cases in the experimental group and 344 cases in the control group. The meta-analysis result indicates that: MBSR can reduce the cancer-related fatigue score of cancer patients, SMD = -0.51,95%CI [-0.81-0.20], P = 0.001, and the difference is statistically significant. The trial sequential analysis indicates that: The RIS required for the indicator to reach the level of significance test should be 1768. The sample size (700 cases) included in the study has not reached the RIS, but it has crossed the traditional threshold and the TSA threshold, indicating that the results tend to be stable. The grading results are shown as low-quality evidence. CONCLUSIONS: This research has used evidence-based medicine to evaluate whether MBSR can alleviate CRF in cancer patients and provide evidence for the comprehensive intervention program for patients with cancer-related fatigue.
Assuntos
Fadiga/terapia , Atenção Plena , Neoplasias/complicações , Estresse Psicológico/terapia , Fadiga/etiologia , Humanos , Neoplasias/psicologiaRESUMO
The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p < 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes - chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor - in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization.
Assuntos
Polpa Dentária/citologia , Células Endoteliais da Veia Umbilical Humana/fisiologia , Peptídeos e Proteínas de Sinalização Intercelular/fisiologia , Neovascularização Fisiológica/fisiologia , Adulto , Análise de Variância , Western Blotting , Ciclo Celular/fisiologia , Ensaios de Migração Celular , Proliferação de Células/fisiologia , Células Cultivadas , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/análise , Masculino , Fator de Crescimento Derivado de Plaquetas/análise , Fator de Crescimento Derivado de Plaquetas/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Receptores CXCR4/análise , Receptores CXCR4/fisiologia , Valores de Referência , Reprodutibilidade dos Testes , Fatores de Tempo , Fator A de Crescimento do Endotélio Vascular/análise , Fator A de Crescimento do Endotélio Vascular/fisiologiaRESUMO
Abstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p < 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes - chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor - in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization.