RESUMO
BACKGROUND: Odontogenic tumours are often biphasic, consisting of epithelial and interstitial components, with an origin that is not well understood. Odontogenic fibromas are rich in mesenchymal component, but also have many epithelial nests. AIMS: To investigate the origin of this tumour by immunohistochemistry. METHODS: The expression of several odontogenic and epithelial markers, including amelogenin, was investigated by immunofluorescent studies. RESULTS: Immunohistochemical analysis showed that epithelial nests exhibited E-cadherin expression, but not amelogenin. Amelogenin positive cells were scattered in the fibrous tissue, which did not exhibit epithelial marker expression except for epithelial membrane antigen. In one case that had received a test biopsy before whole resection of tumour, amelogenin positive cells were distributed in the regenerating mucosal epithelium or subepithelial tissue. CONCLUSIONS: Results indicate that amelogenin positive cells of odontogenic fibromas have an epithelial origin and may have the potential for epithelial mesenchymal transition, which has not to date been investigated in benign tumours.
Assuntos
Amelogenina/metabolismo , Biomarcadores Tumorais/metabolismo , Tumores Odontogênicos/metabolismo , Adulto , Caderinas/metabolismo , Feminino , Humanos , Masculino , Neoplasias Mandibulares/metabolismo , Neoplasias Mandibulares/patologia , Neoplasias Maxilares/metabolismo , Neoplasias Maxilares/patologia , Pessoa de Meia-Idade , Proteínas de Neoplasias/metabolismo , Tumores Odontogênicos/patologiaRESUMO
Novel adenosine antagonists, 2-alkynyl-8-aryl-9-methyladenine derivatives, were synthesized as candidate hypoglycemic agents. These analogues were evaluated for inhibitory activity on N-ethylcarboxamidoadenosine (NECA)-induced glucose production in primary cultured rat hepatocytes. In general, aromatic moieties at the 8-position and alkynyl groups at the 2-position had significantly increased activity compared to unsubstituted compounds. The preferred substituents at the 8-position of adenine were the 2-furyl and 3-fluorophenyl groups. In modifying the alkynyl side chain, change of the ring size, cleavage of the ring, and removal of the hydroxyl group were well tolerated. The order of the stimulatory effects of adenosine agonists on rat hepatocytes was NECA > CPA > CGS21680, which is consistent with involvement of the A(2B) receptor. In Chinese hamster ovary cells stably transfected with human A(2B) receptor cDNA, one of the compounds potent in hepatocytes, 15o (IC(50) = 0.42 microM), antagonized NECA-induced stimulation of cyclic AMP production (IC(50) = 0.063 microM). This inhibitory effect was much more potent than those of FK453, KF17837, and L249313 which have been reported to be respectively A(1), A(2A), and A(3) selective antagonists. These findings agree very well with the result that, compared to 15o, these selective antagonists for each receptor subtype showed only marginal effects in rat hepatocytes. These results suggest that adenosine agonist-induced glucose production in rat hepatocytes is mediated through the A(2B) receptor. Furthermore, 15o showed hypoglycemic activity in an animal model of noninsulin-dependent diabetes mellitus, the KK-A(y) mice. It is possible that inhibition of hepatic glucose production via the A(2B) receptor could be at least one of the mechanisms by which 15o exerts its in vivo effects. Further elaboration of this group of compounds may afford novel antidiabetic agents.
Assuntos
Adenina/análogos & derivados , Adenina/síntese química , Alcinos/síntese química , Glucose/biossíntese , Hipoglicemiantes/síntese química , Fígado/metabolismo , Agonistas do Receptor Purinérgico P1 , Antagonistas de Receptores Purinérgicos P1 , Adenina/química , Adenina/farmacologia , Alcinos/química , Alcinos/farmacologia , Animais , Células CHO , Células Cultivadas , Cricetinae , Diabetes Mellitus/tratamento farmacológico , Diabetes Mellitus/genética , Hepatócitos/metabolismo , Humanos , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Espectroscopia de Ressonância Magnética , Masculino , Espectrometria de Massas , Camundongos , Ensaio Radioligante , Ratos , Receptores Purinérgicos P1/metabolismo , Relação Estrutura-Atividade , TransfecçãoRESUMO
The nephroblastoma overexpressed gene, NOV, is a member of the CCN gene family. We investigated the NOV gene expression pattern in the chicken during early stage embryogenesis. Several embryonic structures showed a distinct expression pattern. The initial expression was detected in Hensen's node (Hamburger and Hamilton stage (HH) 5). The expression was noted in the presumptive notochord and floor plate forming cells. The expression on the left side was more elongated posteriorly, a type of left-right asymmetry. Chicken NOV gene expression in the forming notochord and floor plate was observed until HH 18. The expression was also detected in the ventral area of the mesencephalon and isthmus at HH 14-16.
RESUMO
The effect of topically applied transforming growth factor beta1 (TGF-beta1) on the rat gingival wound healing process after flap surgery was evaluated by immunohistochemistry for extracellular matrix molecules (ECM), such as tenascin, heparan sulfate proteoglycan (HSPG) and type IV collagen, and for proliferating cell nuclear antigen (PCNA) in fibroblasts. TGF-beta1 solution was applied to the surgical wound experimental sites. Two microg/microl were applied at the time of the operation, and 1 microg/microl at days 1 and 2 after surgery, with contralateral control sites receiving the vehicle alone. Periodontal tissues were histologically examined at 3 and 7 days post-surgery. Tenascin was found to be more strongly stained in the granulation tissue from experimental sites at 3 days post-surgery. At 7 days postsurgery, HSPG-positive areas in granulation tissue had become smaller and there was a prominent proliferation of PCNA-positive fibroblast-like cells and type IV collagen-positive blood vessels. These results suggest that TGF-beta1 applied to surgical wounds influences early proliferation of gingival fibroblast-like cells, the formation of blood vessels, and ECM remodelling. In conclusion, TGF-beta1 application appears to promote granulation tissue formation in periodontal wound healing.
Assuntos
Gengiva/fisiologia , Fator de Crescimento Transformador beta/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Colágeno/análise , Colágeno/metabolismo , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/fisiologia , Proteínas da Matriz Extracelular/análise , Proteínas da Matriz Extracelular/metabolismo , Gengiva/irrigação sanguínea , Gengiva/efeitos dos fármacos , Gengiva/cirurgia , Tecido de Granulação/metabolismo , Heparitina Sulfato/análise , Heparitina Sulfato/metabolismo , Imuno-Histoquímica , Masculino , Antígeno Nuclear de Célula em Proliferação/análise , Antígeno Nuclear de Célula em Proliferação/metabolismo , Ratos , Ratos Wistar , Estatísticas não Paramétricas , Tenascina/análise , Tenascina/metabolismo , Fator de Crescimento Transformador beta/fisiologiaRESUMO
Exercise-induced change of nasal resistance and forced expiratory volume in 1 second (FEV1.0) were studied in 30 asthmatic children and seven normal children. Exercise-induced asthma (EIA) was diagnosed in 19 (63%) of the 30 asthmatic patients. Unilateral complete nasal blockage after exercise (exercise-induced nasal obstruction [EINO]) was found in nine (30%) of the 30 asthmatic patients. A marked decrease in nasal resistance took place immediately or 4 minutes after exercise in all cases. EIA is most severe 5 minutes after exercise, and EINO took place 14 or 19 minutes after exercise. The seven normal children had neither EIA nor EINO. The pathophysiologic relationship between EIA and EINO is discussed.
Assuntos
Resistência das Vias Respiratórias , Asma Induzida por Exercício/fisiopatologia , Asma/fisiopatologia , Volume Expiratório Forçado , Adolescente , Criança , Feminino , Humanos , Masculino , Nariz/fisiopatologiaRESUMO
A simple radioenzymatic method for the simultaneous determination of norepinephrine, epinephrine and dopamine in human serum was developed. catecholamines were converted to normetanephrine (NMN), metanephrine (MN), 3-methoxytyramine (3-MT) and their 4-O-methyl isomers by catechol O-methyl transferase with S-adenosylmethionine-(3H-methyl) ([3H]SAM). After addition of unlabelled NMN, MN and 3-MT as carriers, the methyl derivatives were isolated from the reaction mixture by passing through a small boric acid gel column and separated from each other by ion-pair liquid chromatography. Interference by tailing of radioactivity of [3H] SAM and its degradation products eluted earlier was minimized by the use of a precolumn and a branched flow path.